Binding Affinity of Concanavalin A to Native and Acid-Hydrolyzed Phytoglycogen Nanoparticles.

Phytoglycogen (PG) is a polysaccharide produced in the kernels of sweet corn as soft, highly branched, compact nanoparticles. Its tree-like or dendritic architecture, combined with a high-safety profile, makes PG nanoparticles attractive for use in biological applications, many of which rely on the association or binding of small biomolecules. We have developed a methodology to functionalize surface plasmon resonance (SPR) sensor surfaces with PG nanoparticles, and we demonstrate the utility of the PG-functionalized SPR sensor by measuring the binding affinity of the tetrameric concanavalin A (ConA) protein to both native PG nanoparticles and smaller, softer acid-hydrolyzed PG nanoparticles. We measure comparable values of the equilibrium association constant K for native and acid-hydrolyzed PG, with a slightly smaller value for the acid-hydrolyzed particles that we attribute to unfavorable lateral interactions between the tetrameric subunits of ConA due to the increase in surface curvature of the smaller acid-hydrolyzed PG particles. We also use infrared reflection-absorption spectroscopy (IRRAS) to show that ConA maintains a large fraction of its native conformation, and thus its bioactivity, upon binding to PG, representing an important step toward the realization of PG as a novel bioactive delivery vehicle.

Spontaneous Elastocapillary Winding of Thin Elastic Fibers in Contact with Bubbles.

We study the elastocapillary interaction between flexible microfibers in contact with bubbles trapped at the surface of a liquid bath. Microfibers placed on top of bubbles are found to migrate to and wrap into a coil around the perimeter of the bubble for certain bubble-fiber size combinations. The wrapping process is spontaneous: the coil spins atop the bubble, thereby drawing in excess fiber floating on the bath. A two-dimensional microfiber coil emerges which increases the lifetime of the bubbles. A simple model incorporating surface and bending energies captures the spontaneous winding process.

Elastocapillary bending of microfibers around liquid droplets.

We report on the elastocapillary deformation of flexible microfibers in contact with liquid droplets. A fiber is observed to bend more as the size of the contacting droplet is increased. At a critical droplet size, proportional to the bending elastocapillary length, the fiber is seen to spontaneously wind around the droplet. To rationalize these observations, we invoke a minimal model based on elastic beam theory, and find agreement with experimental data. Further energetic considerations provide a consistent prediction for the winding criterion.