Vegetation growth status as an early warning indicator for the spontaneous combustion disaster of coal waste dump after reclamation: An unmanned aerial vehicle remote sensing approach.

Spontaneous combustion of coal waste dumps is a serious threat to the ecological environment and the safety of mining areas. Even after land reclamation and ecological restoration, such spontaneous combustion activities are still active. Achieving early warning of spontaneous combustion is necessary to protect the reclaimed ecosystem and reduce environmental pollution, but it has not yet been well studied. To this end, this study proposed a spatial analysis method to achieve early warning spontaneous combustion of coal waste dump after reclamation by integrating unmanned aerial vehicle (UAV) and vegetation (Medicago sativa/alfalfa) growth status. The experiment was implemented in two slope areas (Areas I and II) of a coal waste dump after reclamation in Shanxi province, China, which were under threat of spontaneous combustion. Three alfalfa growth parameters, aboveground biomass (AGB), plant water content (PWC), and plant height (PH) of the study area, were estimated from UAV imagery features and used to assess the spontaneous combustion risk. Then, soil deep temperature points (25 cm depth) distributed evenly in the study area were collected to determine the underground temperature situation. It was found that the UAV-derived rededge Chlorophyll index (CIrededge), canopy temperature depression (CTD), and canopy height model (CHM) achieved a better estimation of alfalfa AGB (R2 = 0.81, RMSE = 99.2 g/m2, and MAE = 74.9 g/m2), PWC (R2 = 0.68, RMSE = 3.9%, and MAE = 3.2%), and PH (R2 = 0.77, RMSE = 9.79 cm, and MAE = 7.68 cm) of the study area, respectively. We also observed that three alfalfa parameters were highly correlated with the soil deep temperature, but differed in degree (R2 = 0.46-0.81). Furthermore, they were consistent with the soil deep temperature in spatial distribution and could reveal the change direction of underground temperature, which will help us to detect those potential spontaneous combustion areas. These results indicated that vegetation is a prior indicator to the changes in underground temperature of coal waste dump. We believed that UAV can be an effective environmental management tool for the initial assessment of spontaneous combustion risk of coal waste dump after reclamation.

LncRNA TUG1 facilitates proliferation, invasion and stemness of ovarian cancer cell via miR-186-5p/ZEB1 axis.

LncRNA TUG1 has been rarely studied in ovarian cancer (OC), our objective was to explore the role of TUG1 in the regulation of malignant phenotypes of OC. Vectors of sh-TUG1, miR-186-5p and pcDNA-ZEB1 were, respectively, constructed and used to infect OC cells. MTT and transwell assays were applied for representing cell proliferation and invasion, respectively. Sphere formation experiment was used to detect the stemness of OC cells. Western blotting and qRT-PCR were employed for detecting the expression of multiple biomarkers on protein and RNA levels, respectively. The luciferase assay was performed to reveal the interactions between miR-186-5p and TUG1 or ZEB1. The silencing of TUG1 and upregulation of miR-186-5p both suppressed the cell proliferation, invasion and cancer stem cell (CSC) properties. Additionally, luciferase assay verified that miR-186-5p directly binds TUG1 and ZEB1. Moreover, overexpression of ZEB1 rescued the impact on the proliferation, invasion and stemness of TUG1 silencing in OC. TUG1 sponges miR-186-5p to release ZEB1 and promotes the proliferation, invasion and stemness of OC cells, suggesting that TUG1 could be a potential therapeutic target for OC therapy. SIGNIFICANCE OF THE STUDY: LncRNA TUG1 could promote proliferation, invasion and stemness of ovarian cancer cells. Our study first discovered that TUG1 play a tumourigenic role in ovarian cancer by regulating stemness of cancer cells. Mechanism research exhibited the regulation role of TUG1 in ovarian cancer cells was miR-186-5p/ZEB1 axis depended. These results provided a new perspective to understand the pathogenesis and development of ovarian cancer; it will offer new evidence for better diagnosis and treatment therapy of ovarian cancer.

Clinical evaluation of the use of an intracardiac electrocardiogram to guide the tip positioning of peripherally inserted central catheters.

The use of peripherally inserted central catheters (PICCs) provides important central venous accesses for clinical treatments, tests and monitoring. Compared with the traditional methods, intracardiac electrocardiogram (ECG)-guided method has the potential to guide more accurate tip positioning of PICCs. This study aimed to clinically evaluate the effectiveness of an intracardiac ECG to guide the tip positioning by monitoring characteristic P-wave changes. In this study, eligible patients enrolled September 2011 to May 2012 according to the inclusion and exclusion criteria received the catheterization monitored by intracardiac ECG. Then chest radiography was performed to check the catheter position. The results revealed that, with 117 eligible patients, all bar one patient who died (n = 116) completed the study, including 60 males and 56 females aged 51.2 ± 15.1 years. Most (n = 113, > 97%) had characteristic P-wave changes. The intracardiac ECG-guided positioning procedure achieved correct placement for 112 patients (96.56%), demonstrating 99.12% sensitivity and 100% specificity. In conclusion, the intracardiac ECG can be a promising technique to guide tip positioning of PICCs. However, since the sample size in this study is limited, more experience and further study during clinical practice are needed to demonstrate achievement of optimal catheterization outcomes.

Identification of S100A8/A9 involved in thromboangiitis obliterans development using tandem mass tags-labeled quantitative proteomics analysis.

Thromboangiitis obliterans (TAO) is characterized by inflammation and obstruction of small-and medium-sized distal arteries, with limited pharmacotherapies and surgical interventions. The precise pathogenesis of TAO remains elusive. By utilizing the technology of tandem mass tags (TMT) for quantitative proteomics and leveraging bioinformatics tools, a comparative analysis of protein profiles was conducted between normal and TAO rats to identify key proteins driving TAO development. The results unveiled 1385 differentially expressed proteins (DEPs) in the TAO compared with the normal group-comprising 365 proteins with upregulated expression and 1020 proteins with downregulated expression. Function annotation through gene ontology indicated these DEPs mainly involved in cell adhesion, positive regulation of cell migration, and cytosol. The principal signaling pathways involved regulation of the actin cytoskeleton, vascular smooth contraction, and focal adhesion. The roles of these DEPs and associated signaling pathways serve as a fundamental framework for comprehending the mechanisms underpinning the onset and progression of TAO. Furthermore, we conducted a comprehensive evaluation of the effects of S100A8/A9 and its inhibitor, paquinimod, on smooth muscle cells (SMCs) and in TAO rats. We observed that paquinimod reduces SMCs proliferation and migration, promotes phenotype switching and alleviates vascular stenosis in TAO rats. In conclusion, our study revealed that the early activation of S100A8/A9 in the femoral artery is implicated in TAO development, targeting S100A8/A9 signaling may provide a novel approach for TAO prevention and treatment.

Repositioning techniques of malpositioned peripherally inserted central catheters.

AIMS AND OBJECTIVES: To describe potential repositioning techniques of malpositioned peripherally inserted central catheters. BACKGROUND: Various repositioning methods have been applied in clinical practice in managing malpositioned peripherally inserted central catheters, and many of them are proved effective. However, little publication reviewed on those literatures describing repositioning techniques to malpositioned peripherally inserted central catheters. DESIGN: Systematic review. The repositioning techniques were classified and concretely described according to different locations of malpositioned peripherally inserted central catheter tips. METHODS: Literature and relevant guidelines were reviewed, focusing on malpositioned locations and incidence, as well as repositioning skills to peripherally inserted central venous catheters. Six databases were searched, including MEDLINE, web of science, CINAHL, Cochrane library, Wanfang database and Chinese National Knowledge Infrastructure. The articles (n = 21) were analysed using inductive content analysis. RESULTS: The malpositioned locations of postplacement peripherally inserted central catheter tips mainly include right atrium, right ventricular, axillary vein, ipsilateral and contralateral internal jugular vein, subclavian vein, brachiocephalic vein, other small venous branches or catheter looped. Repositioning techniques contained automatic reposition, manual repositioning techniques, re-advancing catheters and catheters' replacement according to different malpositioned patterns. CONCLUSIONS: The most appropriate repositioning techniques should be adopted on the basis of malpositioned locations, direction and length of the malpositioned tip, patients' integrated conditions and available medical equipments to maintain the catheter tip in the best position. RELEVANCE TO CLINICAL PRACTICE: The repositioning techniques described in this review can be applied in clinical practice to ensure the infusion therapy through peripherally inserted central catheter more economical and safe.

Smart Biomimetic Nanozymes for Precise Molecular Imaging: Application and Challenges.

New nanotechnologies for imaging molecules are widely being applied to visualize the expression of specific molecules (e.g., ions, biomarkers) for disease diagnosis. Among various nanoplatforms, nanozymes, which exhibit enzyme-like catalytic activities in vivo, have gained tremendously increasing attention in molecular imaging due to their unique properties such as diverse enzyme-mimicking activities, excellent biocompatibility, ease of surface tenability, and low cost. In addition, by integrating different nanoparticles with superparamagnetic, photoacoustic, fluorescence, and photothermal properties, the nanoenzymes are able to increase the imaging sensitivity and accuracy for better understanding the complexity and the biological process of disease. Moreover, these functions encourage the utilization of nanozymes as therapeutic agents to assist in treatment. In this review, we focus on the applications of nanozymes in molecular imaging and discuss the use of peroxidase (POD), oxidase (OXD), catalase (CAT), and superoxide dismutase (SOD) with different imaging modalities. Further, the applications of nanozymes for cancer treatment, bacterial infection, and inflammation image-guided therapy are discussed. Overall, this review aims to provide a complete reference for research in the interdisciplinary fields of nanotechnology and molecular imaging to promote the advancement and clinical translation of novel biomimetic nanozymes.

A Study of College Teachers' English Teaching Quality Based on Fuzzy Neural Network.

In many universities and colleges, the government is now paying more attention to the quality of teaching assessment, and research on English teaching quality evaluation is becoming increasingly significant. The goal of this paper is to investigate how to use a fuzzy neural network to assess the quality of English education. The research of the model NN and the evaluation of the quality of English teaching is detailed first. A fuzzy NN is an algorithm that examines the quality of English instruction evaluation using fuzzy criteria. In the case results, the data show that the previous evaluation of quality teaching is between the intermediate and the intermediate level, while the research on the evaluation quality of English teaching based on fuzzy NN shows that the evaluation quality is between the intermediate and the advanced level. Therefore, the combination of Fuzzy Rules (FR) and NN methods can effectively improve the quality of college teachers' English teaching evaluation. In college English teaching, FRs can simplify the analysis steps of evaluation of quality teaching, accurately judge the quality of college English teaching evaluation, and provide support for the improvement of evaluation of quality teaching.

Surface water sulfate dynamics in the northern Florida Everglades.

Sulfate contamination has been identified as a serious environmental issue in the Everglades ecosystem. However, it has received less attention compared to P enrichment. Sulfate enters the Arthur R. Marshall Loxahatchee National Wildlife Refuge (Refuge), a remnant of the historic Everglades, in pumped stormwater discharges with a mean concentration of approximately 50 mg L(-1), and marsh interior concentrations at times fall below a detection limit of 0.1 mg L(-1). In this research, we developed a sulfate mass balance model to examine the response of surface water sulfate in the Refuge to changes in sulfate loading and hydrological processes. Meanwhile, sulfate removal resulting from microbial sulfate reduction in the underlying sediments of the marsh was estimated from the apparent settling coefficients incorporated in the model. The model has been calibrated and validated using long-term monitoring data (1995-2006). Statistical analysis indicated that our model is capable of capturing the spatial and temporal variations in surface water sulfate concentrations across the Refuge. This modeling work emphasizes the fact that sulfate from canal discharge is impacting even the interior portions of the Refuge, supporting work by other researchers. In addition, model simulations suggest a condition of sulfate in excess of requirement for microbial sulfate reduction in the Refuge.

Common fixed point theorems for ( T , g ) F -contraction in b-metric-like spaces.

In this paper, we introduce the concept of ( T , g ) F -contraction and investigate some fixed point theorems for such contraction in b-metric-like spaces. Moreover, an example is given to support one of our results, and an application is proposed.

Self-Care Management in Corrections: Perspectives From Persons With an Incarceration Experience.

The purpose of this study was to explore the perceptions and beliefs of what self-care management looks like for a person with an incarceration experience. This is the first phase of a two-phase study. This qualitative study, held in three county jails in Massachusetts, utilized a focus group methodology. The Rediscovery of Self-Care: A Care Intervention for Persons with Incarceration Experience (RSC) model served as the framework for this study. On the basis of a priori constructs from the RSC model, a protocol was established and targeted questions outlined. The results from these focus groups support the constructs of the RSC model. Participants in all focus group interviews reported that self-care was very important and defined self-care, most of the time, in terms of meeting physical needs such as exercising and eating healthy and, more importantly, self-identified mental health and substance use needs such as individual or group counseling. In conclusion, open-ended questions used to identify all instances of potential categories of self-care management supported the central concepts of the RSC model and will inform treatment interventions and modification of an existing self-care management instrument or provide the foundation for the development of a new instrument.