ABSTRACT
Antibiotic-resistant superbug bacteria represent a global health problem with no imminent solutions. Here we demonstrate that the combination (termed AB569) of acidified nitrite (A-NO2-) and Na2-EDTA (disodium ethylenediaminetetraacetic acid) inhibited all Gram-negative and Gram-positive bacteria tested. AB569 was also efficacious at killing the model organism Pseudomonas aeruginosa in biofilms and in a murine chronic lung infection model. AB569 was not toxic to human cell lines at bactericidal concentrations using a basic viability assay. RNA-Seq analyses upon treatment of P. aeruginosa with AB569 revealed a catastrophic loss of the ability to support core pathways encompassing DNA, RNA, protein, ATP biosynthesis, and iron metabolism. Electrochemical analyses elucidated that AB569 produced more stable SNO proteins, potentially explaining one mechanism of bacterial killing. Our data implicate that AB569 is a safe and effective means to kill pathogenic bacteria, suggesting that simple strategies could be applied with highly advantageous therapeutic/toxicity index ratios to pathogens associated with a myriad of periepithelial infections and related disease scenarios.
Subject(s)
Anti-Bacterial Agents/chemistry , Anti-Bacterial Agents/pharmacology , Bacteria/drug effects , Edetic Acid/pharmacology , Sodium Nitrite/pharmacology , Animals , Anti-Bacterial Agents/therapeutic use , Biofilms/drug effects , Disease Models, Animal , Down-Regulation , Drug Resistance, Bacterial/drug effects , Edetic Acid/chemistry , Lung Diseases/drug therapy , Lung Diseases/microbiology , Metabolic Networks and Pathways , Mice , Nitrites/chemistry , Nitrites/pharmacology , Pseudomonas aeruginosa/drug effectsABSTRACT
We present experiments in an in situ fuel cell (FC) inserted in the resonator of the ESR spectrometer that offered the ability to observe separately processes at anode and cathode sides and to monitor the formation of HO and HOO radicals, H and D atoms, and radical fragments derived from the Nafion membrane. The presence of the radicals was determined by spin-trapping electron spin resonance (ESR) with 5,5-dimethylpyrroline N-oxide (DMPO) as a spin trap. The in situ FC was operated at 300 K with a membrane-electrode assembly (MEA) based on Nafion 117 and Pt as catalyst, at closed and open circuit voltage conditions, CCV and OCV, respectively. Experiments with H(2) or D(2) at the anode and O(2) at the cathode were performed. The DMPO/OH adduct was detected only at the cathode for CCV operation, suggesting generation of hydroxyl radicals from H(2)O(2) formed electrochemically via the two-electron reduction of oxygen. The DMPO/OOH adduct, detected in this study for the first time in a FC, appeared at the cathode and anode for OCV operation, and at the cathode after CCV FC operation of >or=2 h. These results were interpreted in terms of electrochemical generation of HOO at the cathode (HO + H(2)O(2) --> H(2)O + HOO) and its chemical generation at the anode from hydrogen atoms and crossover oxygen (H + O(2) --> HOO). DMPO/H and DMPO/D adducts were detected at the anode and cathode sides, for CCV and OCV operation; H and D are aggressive radicals capable of abstracting fluorine from the tertiary carbon in the polymer membrane chain and of leading to chain fragmentation. Carbon-centered radical (CCR) adducts were detected at the cathode after CCV FC operation; weak CCR signals were also detected at the anode. CCRs can originate only from the Nafion membranes, and their presence indicates membrane fragmentation. Taken together, this study has demonstrated that FC operation involves processes such as gas crossover, reactions at the catalyst surface, and possible attack of the membrane by reactive H or D that do not occur in ex situ experiments in the laboratory, thus implying different mechanistic pathways in the two types of experiments.
ABSTRACT
During the last few decades microporous and mesoporous materials have been considered for medical use due to biological properties and stability in biological environment. Zeolites have been investigated as drug carriers, and as adjuvants in anticancer therapy, dietetic supplements or antimicrobial agents. This review gives a brief overview of the major aspects of molecular sieves applications in medicine.
Subject(s)
Drug Carriers/chemistry , Pharmaceutic Aids/chemistry , Zeolites/chemistry , Animals , Antineoplastic Agents/pharmacology , Drug Delivery Systems , Humans , Molecular Mimicry , Polymers , Tissue EngineeringABSTRACT
The ESR spectrum of the chain-end radical RCF2CF2* detected in Nafion perfluorinated membranes exposed to the photo-Fenton reagent was accurately simulated by an automatic fitting procedure, using as input the hyperfine coupling tensors of the two F alpha and two F beta nuclei as well as the corresponding directions of the principal values from density functional theory (DFT) calculations. An accurate fit was obtained only for different orientations of the hyperfine coupling tensors for the two F alpha nuclei, indicating a nonplanar structure about the C alpha radical center. The fitted isotropic hyperfine splittings for the two F beta nuclei in the Nafion radical, 24.9 and 27.5 G, are significantly larger than those for the chain-end radical in Teflon (15 G), implying different radical conformations in the two systems. The excellent fit indicated that the geometry and electronic structure of free radicals can be obtained not only from single-crystal ESR spectroscopy, but also, in certain cases, from powder spectra, by combination with data from DFT calculations. The optimized structures obtained by DFT calculations for the CF3CF2CF2CF2* or CF3OCF2CF2* radicals as models provided additional support for the pyramidal structure determined from the spectral fit. Comparison and analysis of calculated and fitted values for the hyperfine splittings of the two F beta nuclei suggested that the radical detected by ESR in Nafion is ROCF2CF2*, which originates from attack of oxygen radicals on the Nafion side chain. The combination of spectrum fitting and DFT is considered important in terms of understanding the hyperfine splittings from 19F nuclei and the different conformations of fluorinated chain-end-type radicals RCF2CF2* in different systems, and also for elucidating the mechanism of Nafion fragmentation when exposed to oxygen radicals in fuel cell conditions.
ABSTRACT
This review gives a brief overview over the major aspects of application of the nicotine alkaloid and its close derivatives in the therapy of some neurodegenerative disorders and diseases (e.g. Alzheimer's disease, Parkinson's disease, Tourette's syndrome, schizophrenia etc.). The issues concerning methods of nicotine analysis and isolation, and some molecular aspects of nicotine pharmacology are included. The natural and synthetic analogues of nicotine that are considered for medical practice are also mentioned. The molecular properties of two naturally occurring nicotine enantiomers are compared--the less-common but less-toxic (R)-nicotine is suggested as a natural compound that may find its place in pharmaceutical practice.
Subject(s)
Drugs, Investigational/pharmacology , Neurodegenerative Diseases/drug therapy , Nicotiana , Nicotine/pharmacology , Nicotinic Agonists/pharmacology , Animals , Drugs, Investigational/therapeutic use , Humans , Isomerism , Neurodegenerative Diseases/metabolism , Nicotine/analogs & derivatives , Nicotine/isolation & purification , Nicotine/metabolism , Nicotine/therapeutic use , Nicotinic Agonists/isolation & purification , Nicotinic Agonists/metabolism , Nicotinic Agonists/therapeutic use , Receptors, Nicotinic/drug effects , Receptors, Nicotinic/metabolism , Technology, Pharmaceutical/methods , Nicotiana/chemistryABSTRACT
Irradiated samples of deproteinized powdered human bone (femur) have been examined by electron paramagnetic resonance (EPR) spectroscopy in X, Q and W bands. In the bone powder sample only one type of CO2- radical ion is stabilized in the hydroxyapatite structure in contrast to powdered human tooth enamel, a material also containing hydroxyapatite, widely used for EPR dosimetry and in which a few radicals are stable at room temperature. It is suggested that the use of deproteinized bone for EPR dosimetry could improve the accuracy of dose determination.
Subject(s)
Bone and Bones/chemistry , Proteins/isolation & purification , Electron Spin Resonance Spectroscopy , Humans , Microwaves , PowdersABSTRACT
Electron paramagnetic resonance (EPR) spectroscopy was applied to study paramagnetic species stabilized in Na-A zeolite exposed to gaseous methane and gamma-irradiated at 77 K. Two types of EPR spectra were recorded during thermal annealing of zeolite up to room temperature. Owing to the results for the zeolite exposed to (13)CH(4) the multiplet observed at 110 K was assigned to a (.-)CH(3)...Na(+) complex. After decay of the multiplet, the isotropic quartet of methyl radical was recorded in the temperature range of 170-280 K. On the basis of the EPR parameters it is postulated that (.-)CH(3) radicals in this temperature region are able to freely rotate inside the zeolite cage. The structures of the (.-)CH(3)...Na(+) adsorption complex and respective hyperfine coupling constants were calculated by applying DFT quantum chemical methods. Two different models were applied to represent the zeolite framework: the 6T structure of one six-membered ring and the 3T cluster. The hyperfine coupling constants calculated for the (.-)CH(3)...Na(+) adsorption complex for both applied models show very good agreement with those obtained experimentally.
Subject(s)
Gamma Rays , Methane/analogs & derivatives , Sodium/chemistry , Water/chemistry , Zeolites/chemistry , Computer Simulation , Electron Spin Resonance Spectroscopy , Free Radicals/chemistry , Methane/chemistry , Models, Molecular , Molecular ConformationABSTRACT
Electron paramagnetic resonance (EPR) spectroscopy was applied to study the paramagnetic species formed from styrene, 1,1-diphenylethylene, α-methylstyrene, ß-methylstyrene and methylmethacrylate adsorbed on amorphous silica gel after γ-irradiation at 77K. Radicals formed by the hydrogen atom addition at the vinyl group of the monomers were observed in all samples. The hydrogen atoms were shown to originate to a large extent from the adsorbent by using silica gel with deuterated silanol groups. An EPR spectrum assigned to a propagating radical was observed at increased temperature for samples containing methylmethacrylate (MMA). The structures of the adsorption complexes, the respective hyperfine splitting constants and the adsorption energies were calculated by applying DFT quantum chemical methods. The reaction between an MMA molecule and the MMA radical and the structure of the propagating radical was modeled. The calculated hyperfine splitting constants for all radicals confirmed the assignment of the experimental spectra.
Subject(s)
Methylmethacrylate/chemistry , Styrenes/chemistry , Adsorption , Electron Spin Resonance Spectroscopy , Gamma Rays , Models, Molecular , Silicon Dioxide/chemistryABSTRACT
We present in-depth profiling by micro FTIR of cross sections for Nafion 115 membranes in membrane-electrode assemblies (MEAs) degraded during 52 or 180 h at open circuit voltage (OCV) conditions, 90 °C and 30% relative humidity. Analysis of optical images showed highly degraded zones in both MEAs. Corresponding 2D FTIR spectral-spatial maps indicated that C-H and CâO groups are generated during degradation. The highest band intensities for both groups appeared at a depth of 82 µm from the cathode in the MEA degraded for 180 h; the same bands were present but less intense at a depth of 22 µm from the cathode. Degradation at these depths is most likely associated with the location of the Pt band formed from Pt dissolution and migration into the membrane. The two degradation bands, CâO and C-H, appeared at the same depths from the cathode, 82 and 22 µm, suggesting that they are generated by a common mechanism or intermediate. This result is rationalized by a very important first reaction: Abstraction of a fluorine atom from the polymer main chain and side chain by hydrogen atoms, Hâ¢. This step is expected to cause main chain and side chain scission and to generate RF-CF2⢠radicals that can react with H2O2, H2O, and H2 to produce both -COOH and RCF2H groups.