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Biochemistry ; 62(23): 3343-3346, 2023 12 05.
Article in English | MEDLINE | ID: mdl-38009918

ABSTRACT

Family 1 glycosyltransferases (GT1s, UGTs) catalyze the regioselective glycosylation of natural products in a single step. We identified GmUGT88E3 as a particularly promising biocatalyst able to produce a variety of pure, single glycosidic products from polyphenols with high chemical yields. We investigated this particularly desirable duality toward specificity, i.e., promiscuous toward acceptors while regiospecific. Using high-field NMR, kinetic characterization, molecular dynamics simulations, and mutagenesis studies, we uncovered that the main molecular determinant of GmUGT88E3 specificity is a methionine-aromatic bridge, an interaction often present in protein structures but never reported for enzyme-substrate interactions. Here, mutating Met127 led to inactive proteins or 100-fold reduced activity.


Subject(s)
Glycine max , Glycosyltransferases , Glycosyltransferases/metabolism , Glycine max/genetics , Methionine/metabolism , Glycosylation , Glycosides , Racemethionine/metabolism , Substrate Specificity
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