[Automatic determination of urinary mineral phosphate by centrifugation analysis]. / Dosage automatique du phosphate minéral urinaire par analyse centrifuge

Urinary phosphate with acid vanadate and molybdate induces a yellow coloration. The absorbance is proportional to concentration. Kinetics of the reaction including this maximum are measured on Centrifichem; the material and methods, the criteria of validity are presented and discussed.

[Continuous flow determination of plasma uric acid by an enzyme method using a sequential autoanalyzer. Combination with glucose determination in the presence of glucose oxidase]. / Dosage en flux continu de l'acide urique plasmatique par voie enzymatique sur autoanalyseur séquentiel. Association avec le dosage du glucose en présence de glucose oxydase

The simultaneous assays of glucose and uric acid in blood plasma use first a specific oxidase, then a peroxidase and the same chromogenic system. The coupling of the two methods on Autoanalyser SMA 12/60 use five reagents, three of which are identical in the two methods.

[Automatic assay of plasma and urinary calcium, inorganic phosphate, creatinine and uric acid. Comparison between continuous flow analysis on a single microsample and low speed centrifugation]. / Dosage automatique du calcium, du phosphate minéral, de la créatinine et de l'acide urique plasmatiques et urinaires. Comparaison des méthodes en flux continu sur microprélèvement unique et par centrifugation à petite vitesse

A device is described allowing the association on a single microsample of assay by means of continuous flow analysis. The preparation of standards, the evaluation citeria of the method are presented. The preparation of standards, the evaluation criteria of the method are presented. The results are compared to those obtained by low speed centrifugation, i.e. by means of quite different analytical prinicples -- both dynamics and chemicals. These results are discussed : they allow to observe, as a whole, an excellent concordance.

[Automated kinetic assay of plasmatic L-asparaginase activity undergoing therapy for acute lymphoblastic leukemia]. / Dosage automatique en cinétique de l'activité L-asparaginase plasmatique en suivi thérapeutique des leucémies aiguës lymphoblastiques.

The L-asparaginase is a critical drug for the treatment of acute lymphoblastic leukaemia, that achieves blood L-asparagin depletion. However, such a therapy is associated with a high rate of negative side effects, particularly antibody synthesis against L-asparaginase. This therefore decreases therapy efficiency requiring the monitoring of L-asparaginase activity since L-asparagin determination is not easy. We compared here the results obtained with an automated kinetic enzymatic method to those obtained with the most commonly used Nessler reagent method. The correlation coefficient, r = 0,992, obtained was very good, and the allometric regression line was y = 1,038x - 0,37 microkat/L. We also showed that the specificity and the precision were better with the enzymatic method than the Nessler one. Moreover, the enzymatic method was easier and required less time to perform. Finally, the method appears able to perform real time monitoring of the therapy.