ABSTRACT
Lactic acid bacteria are a major component of dairy products, especially species belonging to the genus Lactobacillus. This study reports the whole-genome sequence of Schleiferilactobacillus harbinensis isolated from laban, indigenous fermented milk of Saudi Arabia. The genome sequence is 3,023,618 bp long, has 179 contigs, and has a G+C content of 53.3%.
ABSTRACT
Cronobacter sakazakii is a foodborne pathogen that causes severe illness in neonates and the elderly. Here, we present the genome sequence of C. sakazakii H05 sequence type 156 (ST156), CC21 strain from Kitfo, resulting in a genome size of 4,495,386 bp, with 4,340 coding sequences and a G+C content of 56.85% after assembly and annotation.
ABSTRACT
ABSTRACT: Kitfo is a version of beef tartar widely consumed in the Ethiopian community. It is made from raw minced beef and a blend of powdered spice and butter. Although previous studies have shown that kitfo contains several bacteria that are of public health concern, the status of their antibiotic resistance is not known. In this study, the antibiotic resistance of bacterial isolates from 26 retail kitfo samples obtained from the Washington metropolitan area was analyzed. Characterization and antibiotic sensitivity of the isolates were determined by the Vitek 2 system and pulsed-field gel electrophoresis was used to delineate the intraspecies variations. Of the isolates, 59% were resistant to two or more antibiotics. Acinetobacter calcoaceticus and Pseudomonas luteola were multidrug resistant to the classes of ß-lactam, cephalosporins, and nitrofurantoin. The antibiotic susceptibility profile of the isolates was cefazolin (59%), cefoxitin (50%), ampicillin (32%), and nitrofuran (18%). Most isolates (75%) were Enterobacteriaceae, whereas only 3.8 and 2.6% were Pseudomonadaceae and Moraxellaceae, respectively. Of the Enterobacteriaceae, Enterobacter cloacae, Escherichia coli, and Klebsiella spp. were the most predominant. All isolates except Klebsiella spp. showed high genetic variation (>65%). This study implicates for the first time kitfo as a potential reservoir of antibiotic-resistant bacteria.