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1.
Nature ; 542(7642): 445-449, 2017 02 23.
Article in English | MEDLINE | ID: mdl-28199305

ABSTRACT

A highly protective malaria vaccine would greatly facilitate the prevention and elimination of malaria and containment of drug-resistant parasites. A high level (more than 90%) of protection against malaria in humans has previously been achieved only by immunization with radiation-attenuated Plasmodium falciparum (Pf) sporozoites (PfSPZ) inoculated by mosquitoes; by intravenous injection of aseptic, purified, radiation-attenuated, cryopreserved PfSPZ ('PfSPZ Vaccine'); or by infectious PfSPZ inoculated by mosquitoes to volunteers taking chloroquine or mefloquine (chemoprophylaxis with sporozoites). We assessed immunization by direct venous inoculation of aseptic, purified, cryopreserved, non-irradiated PfSPZ ('PfSPZ Challenge') to malaria-naive, healthy adult volunteers taking chloroquine for antimalarial chemoprophylaxis (vaccine approach denoted as PfSPZ-CVac). Three doses of 5.12 × 104 PfSPZ of PfSPZ Challenge at 28-day intervals were well tolerated and safe, and prevented infection in 9 out of 9 (100%) volunteers who underwent controlled human malaria infection ten weeks after the last dose (group III). Protective efficacy was dependent on dose and regimen. Immunization with 3.2 × 103 (group I) or 1.28 × 104 (group II) PfSPZ protected 3 out of 9 (33%) or 6 out of 9 (67%) volunteers, respectively. Three doses of 5.12 × 104 PfSPZ at five-day intervals protected 5 out of 8 (63%) volunteers. The frequency of Pf-specific polyfunctional CD4 memory T cells was associated with protection. On a 7,455 peptide Pf proteome array, immune sera from at least 5 out of 9 group III vaccinees recognized each of 22 proteins. PfSPZ-CVac is a highly efficacious vaccine candidate; when we are able to optimize the immunization regimen (dose, interval between doses, and drug partner), this vaccine could be used for combination mass drug administration and a mass vaccination program approach to eliminate malaria from geographically defined areas.


Subject(s)
Malaria Vaccines/immunology , Malaria, Falciparum/immunology , Malaria, Falciparum/prevention & control , Plasmodium falciparum/immunology , Vaccines, Attenuated/immunology , Adolescent , Adult , Antibodies, Protozoan/blood , Antibodies, Protozoan/immunology , Chloroquine/therapeutic use , Double-Blind Method , Healthy Volunteers , Humans , Immunologic Memory/immunology , Malaria Vaccines/administration & dosage , Malaria, Falciparum/blood , Malaria, Falciparum/parasitology , Middle Aged , Plasmodium falciparum/classification , Sporozoites/immunology , T-Lymphocytes/immunology , Time Factors , Vaccines, Attenuated/administration & dosage , Young Adult
2.
PLoS One ; 17(8): e0273106, 2022.
Article in English | MEDLINE | ID: mdl-36037183

ABSTRACT

The evaluation of protein antigens as putative serologic biomarkers of infection has increasingly shifted to high-throughput, multiplex approaches such as the protein microarray. In vitro transcription/translation (IVTT) systems-a similarly high-throughput protein expression method-are already widely utilised in the production of protein microarrays, though purified recombinant proteins derived from more traditional whole cell based expression systems also play an important role in biomarker characterisation. Here we have performed a side-by-side comparison of antigen-matched protein targets from an IVTT and purified recombinant system, on the same protein microarray. The magnitude and range of antibody responses to purified recombinants was found to be greater than that of IVTT proteins, and responses between targets from different expression systems did not clearly correlate. However, responses between amino acid sequence-matched targets from each expression system were more closely correlated. Despite the lack of a clear correlation between antigen-matched targets produced in each expression system, our data indicate that protein microarrays produced using either method can be used confidently, in a context dependent manner, though care should be taken when comparing data derived from contrasting approaches.


Subject(s)
Malaria, Falciparum , Plasmodium falciparum , Antibodies, Protozoan , Antibody Formation , Antigens, Protozoan , Biomarkers/metabolism , Humans , Protein Array Analysis , Proteomics , Recombinant Proteins/metabolism
3.
Front Immunol ; 13: 930956, 2022.
Article in English | MEDLINE | ID: mdl-35924245

ABSTRACT

Individuals infected with P. falciparum develop antibody responses to intra-erythrocytic gametocyte proteins and exported gametocyte proteins present on the surface of infected erythrocytes. However, there is currently limited knowledge on the immunogenicity of gametocyte antigens and the specificity of gametocyte-induced antibody responses. In this study, we assessed antibody responses in participants of two controlled human malaria infection (CHMI) studies by ELISA, multiplexed bead-based antibody assays and protein microarray. By comparing antibody responses in participants with and without gametocyte exposure, we aimed to disentangle the antibody response induced by asexual and sexual stage parasites. We showed that after a single malaria infection, a significant anti-sexual stage humoral response is induced in malaria-naïve individuals, even after exposure to relatively low gametocyte densities (up to ~1,600 gametocytes/mL). In contrast to antibody responses to well-characterised asexual blood stage antigens that were detectable by day 21 after infection, responses to sexual stage antigens (including transmission blocking vaccine candidates Pfs48/45 and Pfs230) were only apparent at 51 days after infection. We found antigens previously associated with early gametocyte or anti-gamete immunity were highly represented among responses linked with gametocyte exposure. Our data provide detailed insights on the induction and kinetics of antibody responses to gametocytes and identify novel antigens that elicit antibody responses exclusively in individuals with gametocyte exposure. Our findings provide target identification for serological assays for surveillance of the malaria infectious reservoir, and support vaccine development by describing the antibody response to leading vaccine antigens after primary infection.


Subject(s)
Malaria, Falciparum , Malaria , Antibodies, Protozoan , Humans , Immunity, Humoral , Plasmodium falciparum
4.
Sci Transl Med ; 11(495)2019 06 05.
Article in English | MEDLINE | ID: mdl-31167926

ABSTRACT

The recent decline in global malaria burden has stimulated efforts toward Plasmodium falciparum elimination. Understanding the biology of malaria transmission stages may provide opportunities to reduce or prevent onward transmission to mosquitoes. Immature P. falciparum transmission stages, termed stages I to IV gametocytes, sequester in human bone marrow before release into the circulation as mature stage V gametocytes. This process likely involves interactions between host receptors and potentially immunogenic adhesins on the infected red blood cell (iRBC) surface. Here, we developed a flow cytometry assay to examine immune recognition of live gametocytes of different developmental stages by naturally exposed Malawians. We identified strong antibody recognition of the earliest immature gametocyte-iRBCs (giRBCs) but not mature stage V giRBCs. Candidate surface antigens (n = 30), most of them shared between asexual- and gametocyte-iRBCs, were identified by mass spectrometry and mouse immunizations, as well as correlations between responses by protein microarray and flow cytometry. Naturally acquired responses to a subset of candidate antigens were associated with reduced asexual and gametocyte density, and plasma samples from malaria-infected individuals were able to induce immune clearance of giRBCs in vitro. Infected RBC surface expression of select candidate antigens was validated using specific antibodies, and genetic analysis revealed a subset with minimal variation across strains. Our data demonstrate that humoral immune responses to immature giRBCs and shared iRBC antigens are naturally acquired after malaria exposure. These humoral immune responses may have consequences for malaria transmission potential by clearing developing gametocytes, which could be leveraged for malaria intervention.


Subject(s)
Malaria, Falciparum/immunology , Malaria, Falciparum/parasitology , Plasmodium falciparum/immunology , Animals , Antibodies, Protozoan/immunology , Antibodies, Protozoan/metabolism , Antigens, Protozoan/immunology , Antigens, Protozoan/metabolism , Erythrocytes/parasitology , Flow Cytometry , Humans , Immunoblotting , Malaria/immunology , Malaria/metabolism , Malaria/prevention & control , Malaria, Falciparum/prevention & control , Mice , Microscopy, Fluorescence , Phagocytosis/physiology , Protozoan Proteins/immunology , Protozoan Proteins/metabolism , Tandem Mass Spectrometry
5.
Nat Commun ; 9(1): 558, 2018 02 08.
Article in English | MEDLINE | ID: mdl-29422648

ABSTRACT

Infection with Plasmodium can elicit antibodies that inhibit parasite survival in the mosquito, when they are ingested in an infectious blood meal. Here, we determine the transmission-reducing activity (TRA) of naturally acquired antibodies from 648 malaria-exposed individuals using lab-based mosquito-feeding assays. Transmission inhibition is significantly associated with antibody responses to Pfs48/45, Pfs230, and to 43 novel gametocyte proteins assessed by protein microarray. In field-based mosquito-feeding assays the likelihood and rate of mosquito infection are significantly lower for individuals reactive to Pfs48/45, Pfs230 or to combinations of the novel TRA-associated proteins. We also show that naturally acquired purified antibodies against key transmission-blocking epitopes of Pfs48/45 and Pfs230 are mechanistically involved in TRA, whereas sera depleted of these antibodies retain high-level, complement-independent TRA. Our analysis demonstrates that host antibody responses to gametocyte proteins are associated with reduced malaria transmission efficiency from humans to mosquitoes.


Subject(s)
Malaria, Falciparum/immunology , Malaria, Falciparum/parasitology , Plasmodium falciparum , Adult , Aged , Aged, 80 and over , Burkina Faso/epidemiology , Cameroon/epidemiology , Case-Control Studies , Female , Gambia/epidemiology , Humans , Immunoglobulin G/blood , Malaria, Falciparum/blood , Male , Middle Aged
6.
Nat Commun ; 9(1): 1498, 2018 04 11.
Article in English | MEDLINE | ID: mdl-29643330

ABSTRACT

The original version of this Article contained errors in Fig. 3. In panel a, bars from a chart depicting the percentage of antibody-positive individuals in non-infectious and infectious groups were inadvertently included in place of bars depicting the percentage of infectious individuals, as described in the Article and figure legend. However, the p values reported in the Figure and the resulting conclusions were based on the correct dataset. The corrected Fig. 3a now shows the percentage of infectious individuals in antibody-negative and -positive groups, in both the PDF and HTML versions of the Article. The incorrect and correct versions of Figure 3a are also presented for comparison in the accompanying Publisher Correction as Figure 1.The HTML version of the Article also omitted a link to Supplementary Data 6. The error has now been fixed and Supplementary Data 6 is available to download.

7.
Methods Mol Biol ; 1201: 221-33, 2015.
Article in English | MEDLINE | ID: mdl-25388117

ABSTRACT

The host serological profile to a parasitic infection, such as schistosomiasis, can be used to define potential vaccine and diagnostic targets. Determining the host antibody response using traditional approaches is hindered by the large number of putative antigens in any parasite proteome. Parasite protein microarrays offer the potential for a high-throughput host antibody screen to simplify this task. In order to construct the array, parasite proteins are selected from available genomic sequence and protein databases using bioinformatic tools. Selected open reading frames are PCR amplified, incorporated into a vector for cell-free protein expression, and printed robotically onto glass slides. The protein microarrays can be probed with antisera from infected/immune animals or humans and the antibody reactivity measured with fluorophore labeled antibodies on a confocal laser microarray scanner to identify potential targets for diagnosis or therapeutic or prophylactic intervention.


Subject(s)
Antigens, Protozoan/immunology , Parasites/immunology , Polymerase Chain Reaction/methods , Protein Array Analysis/methods , Animals , Antibody Formation/immunology , Databases, Protein , Genetic Vectors , Open Reading Frames , Parasites/genetics , Protozoan Proteins/genetics , Protozoan Proteins/immunology , RNA, Protozoan/isolation & purification
8.
Am J Trop Med Hyg ; 93(3 Suppl): 16-27, 2015 Sep.
Article in English | MEDLINE | ID: mdl-26259938

ABSTRACT

A blood test that captures cumulative exposure over time and assesses levels of naturally acquired immunity (NAI) would provide a critical tool to monitor the impact of interventions to reduce malaria transmission and broaden our understanding of how NAI develops around the world as a function of age and exposure. This article describes a collaborative effort in multiple International Centers of Excellence in Malaria Research (ICEMRs) to develop such tests using malaria-specific antibody responses as biosignatures of transmission and immunity. The focus is on the use of Plasmodium falciparum and Plasmodium vivax protein microarrays to identify a panel of the most informative antibody responses in diverse malaria-endemic settings representing an unparalleled spectrum of malaria transmission and malaria species mixes before and after interventions to reduce malaria transmission.


Subject(s)
Malaria/transmission , Adaptive Immunity/immunology , Adult , Antibodies, Protozoan/immunology , Antibody Formation/immunology , Antigens, Protozoan/immunology , Biomarkers/blood , Humans , International Cooperation , Malaria/diagnosis , Malaria/immunology , Plasmodium falciparum/immunology , Plasmodium vivax/immunology , Protein Array Analysis
9.
AIDS Res Hum Retroviruses ; 30(1): 37-44, 2014 Jan.
Article in English | MEDLINE | ID: mdl-24134221

ABSTRACT

The role of HIV-1-specific antibody responses in HIV disease progression is complex and would benefit from analysis techniques that examine clusterings of responses. Protein microarray platforms facilitate the simultaneous evaluation of numerous protein-specific antibody responses, though excessive data are cumbersome in analyses. Principal components analysis (PCA) reduces data dimensionality by generating fewer composite variables that maximally account for variance in a dataset. To identify clusters of antibody responses involved in disease control, we investigated the association of HIV-1-specific antibody responses by protein microarray, and assessed their association with disease progression using PCA in a nested cohort design. Associations observed among collections of antibody responses paralleled protein-specific responses. At baseline, greater antibody responses to the transmembrane glycoprotein (TM) and reverse transcriptase (RT) were associated with higher viral loads, while responses to the surface glycoprotein (SU), capsid (CA), matrix (MA), and integrase (IN) proteins were associated with lower viral loads. Over 12 months greater antibody responses were associated with smaller decreases in CD4 count (CA, MA, IN), and reduced likelihood of disease progression (CA, IN). PCA and protein microarray analyses highlighted a collection of HIV-specific antibody responses that together were associated with reduced disease progression, and may not have been identified by examining individual antibody responses. This technique may be useful to explore multifaceted host-disease interactions, such as HIV coinfections.


Subject(s)
Disease Progression , HIV Antibodies/blood , Immunoglobulin G/blood , Principal Component Analysis , Protein Array Analysis , Adult , CD4 Lymphocyte Count , Capsid/immunology , Cross-Sectional Studies , Female , HIV Infections/blood , HIV Infections/immunology , HIV Infections/virology , HIV Integrase/immunology , HIV Reverse Transcriptase/immunology , HIV Seropositivity , HIV-1/immunology , Humans , Kenya , Male , Membrane Glycoproteins/immunology , Molecular Sequence Data , Viral Load/immunology , Viral Matrix Proteins/immunology
10.
Clin Vaccine Immunol ; 18(12): 2148-53, 2011 Dec.
Article in English | MEDLINE | ID: mdl-21976221

ABSTRACT

Two billion people are infected with Mycobacterium tuberculosis, the etiological agent of tuberculosis (TB), worldwide. Ten million to 20 million of the infected individuals develop disease per year. TB is a treatable disease, provided that it is diagnosed in a timely manner. The current TB diagnostic methods are subjective, inefficient, or not cost-effective. Antibody-based blood tests can be used efficiently and cost-effectively for TB diagnosis. A major challenge is that different TB patients generate antibodies against different antigens. Therefore, a multiplex immunoassay approach is needed. We have developed a multiplex panel of 28 M. tuberculosis antigen-coated microbeads. Plasma samples were obtained from over 300 pulmonary TB patients and healthy controls in a country where TB is endemic, Pakistan. Multiplex data were analyzed using computational tools by multivariate statistics, classification algorithms, and cluster analysis. The results of antibody profile-based detection, using 16 selected antigens, closely correlated with those of the sputum-based diagnostic methods (smear microscopy and culture) practiced in countries where TB is endemic. Multiplex microbead immunoassay had a sensitivity and specificity of approximately 90% and 80%, respectively. These antibody profiles could potentially be useful for the diagnosis of nonpulmonary TB, which accounts for approximately 20% of cases of disease. Since an automated, high-throughput version of this multiplex microbead immunoassay could analyze thousands of samples per day, it may be useful for the diagnosis of TB in millions of patients worldwide.


Subject(s)
Antibodies, Bacterial/blood , Biomarkers/blood , Clinical Laboratory Techniques/methods , Mycobacterium tuberculosis/immunology , Tuberculosis/diagnosis , Humans , Immunoassay/methods , Microspheres , Pakistan , Plasma/immunology , Sensitivity and Specificity , Sputum/microbiology
11.
Virology ; 405(1): 31-40, 2010 Sep 15.
Article in English | MEDLINE | ID: mdl-20554302

ABSTRACT

We have developed microarrays with all eight proteins encoded by 13 different human papillomavirus types associated with anogenital cancer (HPV-16, -18, -31, -33, -35, -45, and -53), genital warts (HPV-6 and -11), or skin lesions (HPV-1, -2, -4, and -5). We analyzed the seroprevalence of antibodies in 546 patients, which had either cervical carcinomas, or precursor lesions, or which were asymptomatic. All patient groups contained sera ranging from high reactivity against multiple HPV proteins to low or no reactivity. Computational analyses showed the E7 proteins of carcinogenic HPV types as significantly more reactive in cancer patients compared to asymptomatic individuals and discriminating between cancer and HSIL or LSIL patients. Antibodies against E4 and E5 had the highest seroprevalence but did not exhibit differential reactivity relative to pathology. Our study introduces a new approach to future evaluation of the overall antigenicity of HPV proteins and cross-reaction between homologous proteins.


Subject(s)
Alphapapillomavirus/genetics , Alphapapillomavirus/immunology , Antibodies, Viral/blood , Papillomavirus Infections/immunology , Papillomavirus Infections/virology , Adenocarcinoma/epidemiology , Adenocarcinoma/immunology , Adenocarcinoma/virology , Adult , Alphapapillomavirus/classification , Child, Preschool , Female , Genome, Viral , Humans , Neoplasms, Squamous Cell/epidemiology , Neoplasms, Squamous Cell/immunology , Neoplasms, Squamous Cell/virology , Papillomavirus Infections/epidemiology , Protein Array Analysis , Uterine Cervical Neoplasms/epidemiology , Uterine Cervical Neoplasms/immunology , Uterine Cervical Neoplasms/virology
12.
J Immunol ; 171(10): 4969-73, 2003 Nov 15.
Article in English | MEDLINE | ID: mdl-14607890

ABSTRACT

Memory B cells are a central component of humoral immunity, and yet little is known about their longevity in humans. Immune memory after smallpox vaccination (DryVax) is a valuable benchmark for understanding the longevity of B cell memory in the absence of re-exposure to Ag. In this study, we demonstrate that smallpox vaccine-specific memory B cells last for >50 years in immunized individuals. Virus-specific memory B cells initially declined postimmunization, but then reached a plateau approximately 10-fold lower than peak and were stably maintained for >50 years after vaccination at a frequency of approximately 0.1% of total circulating IgG(+) B cells. These persisting memory B cells were functional and able to mount a robust anamnestic Ab response upon revaccination. Additionally, virus-specific CD4(+) T cells were detected decades after vaccination. These data show that immunological memory to DryVax vaccine is long-lived and may contribute to protection against smallpox.


Subject(s)
B-Lymphocyte Subsets/cytology , B-Lymphocyte Subsets/immunology , Immunologic Memory , Smallpox Vaccine/administration & dosage , Smallpox Vaccine/immunology , Adolescent , Adult , Antibodies, Viral/blood , B-Lymphocyte Subsets/metabolism , Cell Survival/immunology , Cells, Cultured , Child , Female , Humans , Immunization Schedule , Male , Middle Aged , Vaccines, Attenuated/administration & dosage , Vaccines, Attenuated/immunology , Vaccinia virus/immunology
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