Theama japonica sp. nov., an Interstitial Polyclad Flatworm Showing a Wide Distribution along Japanese Coasts.

We establish a new interstitial polyclad species, Theama japonica sp. nov., based on specimens collected from coarse-sandy habitats in three Japanese main islands (Hokkaido, Honshu, and Shikoku) along the coasts of the Pacific Ocean and the Sea of Japan. Theama japonica is characterized by i) two pairs of cerebral eyespots and four to six precerebral eyespots; ii) eosinophilic secretion glands distributed in the distal half of the inner ventral part of the prostatic vesicle; iii) a conical penis papilla, bent up dorsally, with a sclerotized inner wall; iv) the prostatic sheath with an inner angular fold on the dorso-distal side; and v) the external cilia longer dorsally than ventrally. Partial sequences of the cytochrome c oxidase subunit I (COI) gene from 20 specimens collected at eight localities along Japanese coasts represented 19 haplotypes. The uncorrected p-distances among these COI haplotypes fell within intraspecific variations observed in other polyclads. A network analysis based on these COI haplotypes suggested a geographically non-cohesive genetic structure of the species, possibly indicating the species' high dispersibility. Molecular phylogenetic analyses based on a concatenated dataset of 18S and 28S rDNA sequences showed T. japonica formed a clade with other Theama species. The resulting tree also indicates that our new species is more closely related to Theama sp. from Colombia than species from Panama and Croatia.

A new Stygarctus (Arthrotardigrada: Stygarctidae) from Japan, with entangled seminal receptacle ducts.

Stygarctus ayatori sp. nov. (Arthrotardigrada: Stygarctidae), is described from a sandy beach located at Okinoshima, Tateyama Bay, Boso Peninsula, Honshu, Japan. The most prominent characters were observed in the female genital structure, with the seminal receptacle ducts forming a three-dimensional entanglement near the exterior opening, and internal thickening situated peripheral to the gonopore and between the gonopore and anus. The new species is distinguished from the congeners by these characters; excluding S. abornatus McKirdy et al., 1976 for which there is no information on the genital structure. However, S. ayatori sp. nov. and S. abornatus can be differentiated by the presence of dorsal spines on the former species, which are absent from the latter.

Correction to: 'Revisiting Stygocapitella (Annelida, Parergodrilidae) in Japan, with insights into their amphi-Pacific diversification' (2024), by Natsumi Hookabe et al.

[This corrects the article DOI: 10.1098/rsos.231782.].

Revisiting Stygocapitella (Annelida, Parergodrilidae) in Japan, with insights into their amphi-Pacific diversification.

Polychaetes are typically found in marine environments with limited species adapting to semi-terrestrial habitats. The genus Stygocapitella comprises interstitial polychaetes dwelling in sandy beach areas around or above the high-water line. Based on molecular data, previous studies suggested the presence of multiple cryptic species in some different localities in the world lumped together as Stygocapitella subterranea. In Japan, reports on Stygocapitella were scarce, with only one species having been documented 40 years ago at Ishikari Beach in Hokkaido by the name of S. subterranea. We revisited these earlier findings and uncovered the presence of two distinct species in Stygocapitella. One of these species is herein named Stygocapitella itoi sp. nov., while the other corresponds to S. budaevae, originally described from the Russian Far East. Stygocapitella itoi sp. nov. possesses a chaetal pattern similar to that of S. australis, S. furcata and S. pacifica but can be distinguished from the congeners by two characters: a slightly forked pygidium and forked chaetae consisting of two teeth and two outer prongs. Our multi-locus phylogenetic analysis showed close relationships across the Pacific Ocean in two separated lineages in the genus, suggesting ancient dispersal or allopatric speciation after vicariance events.

Neostygarctus lovedeluxe n. sp. from the Miyako Islands, Japan: the first record of Neostygarctidae (Heterotardigrada: Arthrotardigrada) from the Pacific.

A new species of the previously monospecific marine tardigrade family Neostygarctidae is described. Neostygarctus lovedeluxe n. sp. was found from a submarine cave in Miyako Islands, Japan. This is the first record of Neostygarctidae from the Pacific. The new species is easily distinguished from the previously known N. acanthophorus by its number of dorsal spines, as N. lovedeluxe has two spines each on the three dorsal body plates in contrast to one in N. acanthophorus. Furthermore the morphology of the two clawed juvenile is reported for the first time in Neostygarctidae, providing new insights into the common sequence in some ontogenic traits.

Four new species of Ctenodrilus, Raphidrilus, and Raricirrus (Cirratuliformia, Annelida) in Japanese waters, with notes on their phylogenetic position.

Four new species of annelids, Ctenodrilus japonicus sp. nov., Raphidrilus misakiensis sp. nov., Raphidrilus okinawaensis sp. nov., and Raricirrus anubis sp. nov., are described based on specimens collected from Japanese waters. Ctenodrilus japonicus sp. nov. inhabits the interstitial environment and can be distinguished from the other congeners by the following features: (i) total of 16 chaetigers, (ii) chaetigers 1-3 with stout hooks, (iii) minute body (approximately 1 mm in length), (iv) all parapodia with the same number of chaetae (two notochaetae; two neurochaetae), and (v) presence of dorsal and ventral papillae. Raphidrilus misakiensis sp. nov. lives under intertidal stones and can be distinguished from other congeners by having pectinate neurochaetae. Raphidrilus okinawaensis sp. nov. inhabits the interstitial environment and can be distinguished from other congeners by: (i) absence of annulation on the peristomium and achaetous segment and (ii) presence of a heart body in chaetigers 4-5. Raricirrus anubis sp. nov. inhabits whale bones and can be distinguished from other congeners by the following features: (i) presence of a heart body in chaetigers 9-14, (ii) presence of capillary neurochaetae on chaetiger 1, and (iii) presence of simple curved spines. A phylogenetic tree based on partial sequences of cytochrome c oxidase subunit I and 16S rRNA from the new species and other cirratulid worms showed that Raphidrilus is included in Cirratuliformia. This is the first record of Raphidrilus and Raricirrus from Japanese waters.

Black spicules from a new interstitial opheliid polychaete Thoracophelia minuta sp. nov. (Annelida: Opheliidae).

The phylum Annelida exhibits high morphological diversity coupled with its extensive ecological diversity, and the process of its evolution has been an attractive research subject for many researchers. Its representatives are also extensively studied in fields of ecology and developmental biology and important in many other biology related disciplines. The study of biomineralisation is one of them. Some annelid groups are well known to form calcified tubes but other forms of biomineralisation are also known. Herein, we report a new interstitial annelid species with black spicules, Thoracophelia minuta sp. nov., from Yoichi, Hokkaido, Japan. Spicules are minute calcium carbonate inclusions found across the body and in this new species, numerous black rod-like inclusions of calcium-rich composition are distributed in the coelomic cavity. The new species can be distinguished from other known species of the genus by these conspicuous spicules, shape of branchiae and body formula. Further, the new species' body size is apparently smaller than its congeners. Based on our molecular phylogenetic analysis using 18S and 28S sequences, we discuss the evolutionary significance of the new species' spicules and also the species' progenetic origin.

Two new species of Fauveliopsidae (Annelida, Sedentaria) from submarine caves and deep sea, Japan.

Two new species of fauveliopsid annelids, Fauveliopsis antri sp. nov. and Laubieriopsis soyoae sp. nov., are described based on specimens collected from Japanese waters. Fauveliopsis antri sp. nov. inhabits submarine caves and can be discriminated from the other congeners by the following features: i) 32 chaetigers, ii) chaetigers 1-3 with stout hooks, iii) minute body (about 1 mm in length), iv) all parapodia with same number of chaetae (2 notochaetae; 2 neurochaetae), and v) presence of dorsal and ventral papillae. Laubieriopsis soyoae sp. nov. inhabits deep water sediments and can be distinguished from the other congeners by: i) 24 chaetigers, ii) chaetigers 1-3 without annulations, and iii) acicular chaetae with tips entire, without denticles.

Halechiniscidae (Heterotardigrada, Arthrotardigrada) of Oura Bay, Okinawajima, Ryukyu Islands, with descriptions of three new species.

Marine tardigrades of the family Halechiniscidae (Heterotardigrada: Arthrotardigrada) are reported from Oura Bay, Okinawajima, one of the Ryukyu Islands, Japan, including Dipodarctus sp., Florarctuswunai sp. n., Halechiniscuschurakaagii sp. n., Halechiniscusyanakaagii sp. n. and Styraconyx sp. The attributes distinguishing Florarctuswunai sp. n. from its congeners is a combination of two characters, the smooth dorsal cuticle and two small projections of the caudal alae caestus. Halechiniscuschurakaagii sp. n. is differentiated from its congeners by the combination of two characters, the robust cephalic cirrophores and the scapular processes with flat oval tips, while Halechiniscusyanakaagii sp. n. can be identified by the laterally protruded arched double processes with acute tips situated dorsally at the level of leg I. A list of marine tardigrades reported from the Ryukyu Islands is provided.

Quisarctus yasumurai gen. et sp. nov. (Arthrotardigrada: Halechiniscidae) from a submarine cave, off Iejima, Ryukyu Islands, Japan.

Quisarctus yasumurai gen. et sp. nov. (Arthrotardigrada: Halechiniscidae) is described from the submarine cave 'Daidokutsu', off Iejima, Okinawa Islands, Ryukyu Islands, Japan. It is characterised by a cylindrical body, long primary clava and shorter lateral cirrus inserted on a common cirrophore, and simple digits of unequal lengths (without folds, peduncles, proximal pads, pretarsi, or wrinkles) that terminate in a sheathed, small, crescent-shaped claw with a minute calcar. Quisarctinae subfam. nov. is erected for this unique new genus.

Phylogenetic position of Loricifera inferred from nearly complete 18S and 28S rRNA gene sequences.

BACKGROUND: Loricifera is an enigmatic metazoan phylum; its morphology appeared to place it with Priapulida and Kinorhyncha in the group Scalidophora which, along with Nematoida (Nematoda and Nematomorpha), comprised the group Cycloneuralia. Scarce molecular data have suggested an alternative phylogenetic hypothesis, that the phylum Loricifera is a sister taxon to Nematomorpha, although the actual phylogenetic position of the phylum remains unclear. METHODS: Ecdysozoan phylogeny was reconstructed through maximum-likelihood (ML) and Bayesian inference (BI) analyses of nuclear 18S and 28S rRNA gene sequences from 60 species representing all eight ecdysozoan phyla, and including a newly collected loriciferan species. RESULTS: Ecdysozoa comprised two clades with high support values in both the ML and BI trees. One consisted of Priapulida and Kinorhyncha, and the other of Loricifera, Nematoida, and Panarthropoda (Tardigrada, Onychophora, and Arthropoda). The relationships between Loricifera, Nematoida, and Panarthropoda were not well resolved. CONCLUSIONS: Loricifera appears to be closely related to Nematoida and Panarthropoda, rather than grouping with Priapulida and Kinorhyncha, as had been suggested by previous studies. Thus, both Scalidophora and Cycloneuralia are a polyphyletic or paraphyletic groups. In addition, Loricifera and Nematomorpha did not emerge as sister groups.

Two new species in the Echinoderes coulli group (Echinoderidae, Cyclorhagida, Kinorhyncha) from the Ryukyu Islands, Japan.

Two new species belonging to the Echinoderes coulli group are described with their external morphologies and sequences of nuclear 18S rRNA and 28S rRNA genes, and mitochondrial COI gene. The first species, Echinoderes komatsui sp. n., is characterized by absence of acicular spines, and presence of lateroventral tubules on segments 5 and 8, laterodorsal tubules on segment 10, inverted triangle or wide oval shaped large sieve plates, lateral terminal accessory spines in female, and short tips of ventral pectinate fringe on segment 10. The second species, Echinoderes hwiizaa sp. n., is characterized by absence of acicular spines, and presence of lateroventral tubules on segments 5 and 7-9, midlateral tubules on segment 8, laterodorsal tubules on segment 10, large narrow oval shaped sieve plates on segment 9, and thick, short and blunt lateral terminal spines about 10-15% of trunk length. The diagnostic characters and key to species of E. coulli group are provided as well.