ABSTRACT
By integrating the microarray expression data and a global E. faecalis transcriptional network we identified a sub-network activated by zinc and copper. Our analyses indicated that the transcriptional response of the bacterium to copper and zinc exposure involved the activation of two modules, module I that contains genes implicated in zinc homeostasis, including the Zur transcriptional repressor, and module II containing a set of genes associated with general stress response and basal metabolism. Bacterial exposure to zinc and copper led to the repression of the zinc uptake systems of module I. Upon deletion of Zur, exposure to different zinc and copper conditions induced complementary homeostatic mechanisms (ATPase efflux proteins) to control the intracellular concentrations of zinc. The transcriptional activation of zinc homeostasis genes by zinc and copper reveals a functional interplay between these two metals, in which exposure to copper also impacts on the zinc homeostasis. Finally, we present a new zinc homeostasis model in E. faecalis, positioning this bacterium as one of the most complete systems biology model in metals described to date.
Subject(s)
Bacterial Proteins/genetics , Copper/metabolism , Enterococcus faecalis/genetics , Gene Expression Regulation, Bacterial , Repressor Proteins/genetics , Zinc/metabolism , Amino Acid Sequence , Bacterial Proteins/chemistry , Bacterial Proteins/metabolism , Enterococcus faecalis/chemistry , Enterococcus faecalis/metabolism , Gram-Positive Bacterial Infections/microbiology , Homeostasis , Humans , Models, Molecular , Molecular Sequence Data , Repressor Proteins/chemistry , Repressor Proteins/metabolism , Sequence AlignmentABSTRACT
Objetivo: comunicar nuestra experiencia en eldesarrollo de la flebectomía venosa.Materiales y métodos: entre Marzo de 2007 y Abrilde 2009 en el Servicio de Flebología y Linfología delHospital Nacional de Clínicas de la ciudad de Córdoba,se realizó un estudio con el objetivo de describir la técni-ca quirúrgica de la mini-flebectomía venosa, se evalua-ron ade m ás los resu ltad os, las com p lica cion espostoperatorias y la tasa de recidivas. Resultados: Latasa de recidivas fue de 0% para el sector venoso inter-venido. Con respecto a las complicaciones encontra-mos un 1,68% de pigmentación en el sitio de la incisión,hematomas en un 2,52%, parestesias en un 3,36% delos casos, infección local en 0,84% de los pacientes,induración subcutánea en 6,72% de los casos, depre-sión sobre la cicatriz en 0,84% y no se presentaron ca-sos de cicatriz dolorosa. Conclusiones: Nos encontra-mos ante la presencia de un método extensamente pro-bado en su eficacia, seguridad, inocuidad, con una ele-vada tasa de satisfacción para los pacientes, que permi-te una rápida reinserción laboral, con una tasa despre-ciable de complicaciones.
Objective: To re port our e xperience in the development of venous vein flebectomy.Materials and mthoeds: between March 2007 and April 2009 in the Departm ent of Phlebolo gy and Lymphology National Clinical Hospital of the city ofCordoba, a study was conducted in order to describe the surgical technique of mini-phlebectomy vein was alsoassessed findings, postoperative complications and recurrence rate. Results: The recurrence rate was 0%for the venous intervened. With regard to complications we found a 1.68% of pigmentation at the site of the incision,bruising or 2.52%, 3.36% paresthesias cases, local infection in 0.84% of patients, subcutaneous induration6.72% of cases, depression of the scar in 0.84% andthere were no cases of painful scar. Conclusions: We arein the presence of an extensively tested methodeffectiveness, safety, safety, with a high rate of satisfactionfor patients, allowing a quick return to work, with anegligible rate of complications.