ABSTRACT
BACKGROUND: Aim of the study was to compare 4 different methods of sterilizing endodontic files in dental practice. MATERIALS & METHODS: The present study was performed on 100 K-files, 21 mm long and of size 25. Of these, 20 files were taken as control group, and the remaining 80 files were divided into 4 groups of 20 files each and they were tested for the efficacy of sterilization with different methods: Autoclave, glass bead, glutaraldehyde and CO2 laser. RESULTS: The study showed that the files sterilized by autoclave and lasers were completely sterile. Those sterilized by glass bead were 90% sterile and those with glutaraldehyde were 80% sterile. CONCLUSION: The study concluded that autoclave or laser could be used as a method of sterilization in clinical practice and in advanced clinics; laser can be used also as a chair side method of sterilization. How to cite this article: Raju TB, Garapati S, Agrawal R, Reddy S, Razdan A, Kumar SK. Sterilizing Endodontic Files by four different sterilization methods to prevent cross-infection - An In-vitro Study. J Int Oral Health 2013; 5(6):108-12 .