Association of -604G/A and -501A/C Ghrelin and Obestatin Prepropeptide Gene Polymorphisms with Polycystic Ovary Syndrome.

Ghrelin hormone has an important role in a wide range of metabolic and non-metabolic processes. Polymorphisms of ghrelin gene could be associated with a large number of diseases. The aim of this study was to evaluate the association of -604G/A and -501A/C polymorphisms in ghrelin and obestatin prepropeptide gene (GHRL) with polycystic ovary syndrome (PCOS) in a sample of Iranian women. One hundred and fifty-two women with PCOS and 162 age-matched apparently healthy women as control group were enrolled in this study. The study subjects were genotyped for polymorphisms in the ghrelin gene using polymerase chain reaction-restriction fragment length polymorphism-based methods. Biochemical parameters, serum prolactin, luteinizing hormone, follicle stimulating hormone, estradiol, and testosterone were estimated by chemiluminescence assay. Serum lipids and lipoproteins were determined by standard enzymatic methods. The association between the risk of PCOS and ghrelin gene polymorphisms was examined using Multivariate analysis. The frequency of the -604G/A and -501A/C polymorphisms was not statistically different between patients and the control group of women (p = 0.12 and p = 0.21, respectively). A significantly higher level of LDL-C was found in the wild-type AA genotype compared with CC genotype of -501A/C polymorphism (p = 0.02). Our findings indicate that neither -604G/A and nor -501A/C polymorphisms of ghrelin gene are associated with PCOS, but suggest a relation between the presence of polymorphic allele of -501A/C polymorphism and LDL-C level in a sample of Iranian women.

Lack of association between LIPC-514 C/T polymorphism of hepatic lipase and endometriosis in Iranian women.

AIM: Patients with endometriosis may suffer from dyslipidemia. Hepatic lipase (HL) is involved in the metabolism of lipoproteins and has an important role in reverse cholesterol transport. The aim of this study was to investigate the association between the LIPC-514 C/T polymorphism in the HL gene and the risk of endometriosis in a group of Iranian women. METHODS: Ninety-seven patients with endometriosis and 107 women who were negative for endometriosis after diagnostic laparoscopy, as control group, were enrolled in this cross-sectional study. Samples were analyzed for polymorphism of the HL gene using polymerase chain reaction restriction fragment length polymorphism. RESULTS: Multivariate analysis was used to examine the association between the risk of endometriosis and LIPC-514 C/T polymorphism. There was no statistically significant difference in the frequency of the LIPC-514 C/T polymorphism between patients and the controls (60.7% CC, 34.6% CT, 4.7% TT versus 68.4%, 27.4%, 4.2%, respectively, P = 0.52). CONCLUSION: The present study suggested that the LIPC-514 C/T polymorphism of the HL gene has no significant association with the risk of endometriosis in the studied Iranian women.

I405V polymorphism of CETP gene and lipid profile in women with endometriosis.

Genetic factors have an important role in the pathophysiology of endometriosis. In addition, abnormalities in lipid profile and intrinsic inflammatory status are associated with disease progression. The purpose of this study was to evaluate the effect of the I405V polymorphism of cholesteryl ester transfer protein (CETP) gene and lipid profile with the risk of endometriosis in women. Ninety-seven women with laparoscopy-diagnosed endometriosis were recruited for this study, and 107 patients with no evidence of endometriosis confirmed by laparoscopy served as controls. Samples were analyzed for polymorphism of the CETP gene using polymerase chain reaction-restriction fragment length polymorphism-based methods. After adjustment for body mass index, high-density lipoprotein-C and low-density lipoprotein-C, the risk of endometriosis in patients with normal genotype homozygous was more of the rare allele (p < 0.001, odds ratio = 0.21, 95% confidence interval = 0.09-0.45). Our results suggest that I405V polymorphism of CETP gene plays an important role as independent factor in the risk of endometriosis in women.