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1.
Cell ; 165(3): 690-703, 2016 Apr 21.
Article in English | MEDLINE | ID: mdl-27062925

ABSTRACT

Pili are proteinaceous polymers of linked pilins that protrude from the cell surface of many bacteria and often mediate adherence and virulence. We investigated a set of 20 Bacteroidia pilins from the human microbiome whose structures and mechanism of assembly were unknown. Crystal structures and biochemical data revealed a diverse protein superfamily with a common Greek-key ß sandwich fold with two transthyretin-like repeats that polymerize into a pilus through a strand-exchange mechanism. The assembly mechanism of the central, structural pilins involves proteinase-assisted removal of their N-terminal ß strand, creating an extended hydrophobic groove that binds the C-terminal donor strands of the incoming pilin. Accessory pilins at the tip and base have unique structural features specific to their location, allowing initiation or termination of the assembly. The Bacteroidia pilus, therefore, has a biogenesis mechanism that is distinct from other known pili and likely represents a different type of bacterial pilus.


Subject(s)
Fimbriae Proteins/chemistry , Fimbriae, Bacterial , Gastrointestinal Microbiome , Amino Acid Sequence , Crystallography, X-Ray , Fimbriae Proteins/genetics , Fimbriae Proteins/metabolism , Humans , Lipoproteins/chemistry , Lipoproteins/metabolism , Models, Molecular , Molecular Sequence Data , Sequence Alignment
2.
Proc Natl Acad Sci U S A ; 120(18): e2216342120, 2023 05 02.
Article in English | MEDLINE | ID: mdl-37098070

ABSTRACT

NKG2D (natural-killer group 2, member D) is a homodimeric transmembrane receptor that plays an important role in NK, γδ+, and CD8+ T cell-mediated immune responses to environmental stressors such as viral or bacterial infections and oxidative stress. However, aberrant NKG2D signaling has also been associated with chronic inflammatory and autoimmune diseases, and as such NKG2D is thought to be an attractive target for immune intervention. Here, we describe a comprehensive small-molecule hit identification strategy and two distinct series of protein-protein interaction inhibitors of NKG2D. Although the hits are chemically distinct, they share a unique allosteric mechanism of disrupting ligand binding by accessing a cryptic pocket and causing the two monomers of the NKG2D dimer to open apart and twist relative to one another. Leveraging a suite of biochemical and cell-based assays coupled with structure-based drug design, we established tractable structure-activity relationships with one of the chemical series and successfully improved both the potency and physicochemical properties. Together, we demonstrate that it is possible, albeit challenging, to disrupt the interaction between NKG2D and multiple protein ligands with a single molecule through allosteric modulation of the NKG2D receptor dimer/ligand interface.


Subject(s)
Killer Cells, Natural , NK Cell Lectin-Like Receptor Subfamily K , Ligands , CD8-Positive T-Lymphocytes , Protein Binding
3.
Bioorg Med Chem Lett ; 96: 129492, 2023 11 15.
Article in English | MEDLINE | ID: mdl-37778428

ABSTRACT

Natural killer group 2D (NKG2D) is a homodimeric activating immunoreceptor whose function is to detect and eliminate compromised cells upon binding to the NKG2D ligands (NKG2DL) major histocompatibility complex (MHC) molecules class I-related chain A (MICA) and B (MICB) and UL16 binding proteins (ULBP1-6). While typically present at low levels in healthy cells and tissue, NKG2DL expression can be induced by viral infection, cellular stress or transformation. Aberrant activity along the NKG2D/NKG2DL axis has been associated with autoimmune diseases due to the increased expression of NKG2D ligands in human disease tissue, making NKG2D inhibitors an attractive target for immunomodulation. Herein we describe the discovery and optimization of small molecule PPI (protein-protein interaction) inhibitors of NKG2D/NKG2DL. Rapid SAR was guided by structure-based drug design and accomplished by iterative singleton and parallel medicinal chemistry synthesis. These efforts resulted in the identification of several potent analogs (14, 21, 30, 45) with functional activity and improved LLE.


Subject(s)
Carrier Proteins , NK Cell Lectin-Like Receptor Subfamily K , Humans , NK Cell Lectin-Like Receptor Subfamily K/metabolism , Carrier Proteins/metabolism , Histocompatibility Antigens Class I/metabolism , Protein Binding , Killer Cells, Natural/metabolism , Ligands
4.
J Biol Chem ; 288(23): 16789-16799, 2013 Jun 07.
Article in English | MEDLINE | ID: mdl-23572527

ABSTRACT

DUF2233, a domain of unknown function (DUF), is present in many bacterial and several viral proteins and was also identified in the mammalian transmembrane glycoprotein N-acetylglucosamine-1-phosphodiester α-N-acetylglucosaminidase ("uncovering enzyme" (UCE)). We report the crystal structure of BACOVA_00430, a 315-residue protein from the human gut bacterium Bacteroides ovatus that is the first structural representative of the DUF2233 protein family. A notable feature of this structure is the presence of a surface cavity that is populated by residues that are highly conserved across the entire family. The crystal structure was used to model the luminal portion of human UCE (hUCE), which is involved in targeting of lysosomal enzymes. Mutational analysis of several residues in a highly conserved surface cavity of hUCE revealed that they are essential for function. The bacterial enzyme (BACOVA_00430) has ∼1% of the catalytic activity of hUCE toward the substrate GlcNAc-P-mannose, the precursor of the Man-6-P lysosomal targeting signal. GlcNAc-1-P is a poor substrate for both enzymes. We conclude that, for at least a subset of proteins in this family, DUF2233 functions as a phosphodiester glycosidase.


Subject(s)
Bacterial Proteins/chemistry , Bacteroides/enzymology , Phosphoric Diester Hydrolases/chemistry , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Catalytic Domain , Crystallography, X-Ray , Humans , Mutagenesis , Phosphoric Diester Hydrolases/genetics , Phosphoric Diester Hydrolases/metabolism , Structural Homology, Protein
5.
Proteins ; 82(6): 1086-92, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24174223

ABSTRACT

Pseudomonas aeruginosa is an opportunistic pathogen commonly found in humans and other organisms and is an important cause of infection especially in patients with compromised immune defense mechanisms. The PA3611 gene of P. aeruginosa PAO1 encodes a secreted protein of unknown function, which has been recently classified into a small Pseudomonas-specific protein family called DUF4146. As part of our effort to extend structural coverage of novel protein space and provide a structure-based functional insight into new protein families, we report the crystal structure of PA3611, the first structural representative of the DUF4146 protein family.


Subject(s)
Bacterial Proteins/chemistry , Pseudomonas aeruginosa , Amino Acid Sequence , Conserved Sequence , Crystallography, X-Ray , Models, Molecular , Protein Structure, Secondary , Quorum Sensing
6.
Acta Crystallogr D Biol Crystallogr ; 70(Pt 10): 2640-51, 2014 Oct.
Article in English | MEDLINE | ID: mdl-25286848

ABSTRACT

The crystal structure of arabinose-5-phosphate isomerase (API) from Bacteroides fragilis (bfAPI) was determined at 1.7 Šresolution and was found to be a tetramer of a single-domain sugar isomerase (SIS) with an endogenous ligand, CMP-Kdo (cytidine 5'-monophosphate-3-deoxy-D-manno-oct-2-ulosonate), bound at the active site. API catalyzes the reversible isomerization of D-ribulose 5-phosphate to D-arabinose 5-phosphate in the first step of the Kdo biosynthetic pathway. Interestingly, the bound CMP-Kdo is neither the substrate nor the product of the reaction catalyzed by API, but corresponds to the end product in the Kdo biosynthetic pathway and presumably acts as a feedback inhibitor for bfAPI. The active site of each monomer is located in a surface cleft at the tetramer interface between three monomers and consists of His79 and His186 from two different adjacent monomers and a Ser/Thr-rich region, all of which are highly conserved across APIs. Structure and sequence analyses indicate that His79 and His186 may play important catalytic roles in the isomerization reaction. CMP-Kdo mimetics could therefore serve as potent and specific inhibitors of API and provide broad protection against many different bacterial infections.


Subject(s)
Aldose-Ketose Isomerases/chemistry , Aldose-Ketose Isomerases/metabolism , Bacteroides fragilis/chemistry , Aldose-Ketose Isomerases/genetics , Amino Acid Sequence , Bacterial Proteins/chemistry , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Catalytic Domain , Crystallography, X-Ray , Cytidine Monophosphate/analogs & derivatives , Cytidine Monophosphate/chemistry , Histidine/chemistry , Models, Molecular , Molecular Sequence Data , Protein Conformation , Sequence Homology, Amino Acid , Sugar Acids/chemistry
7.
J Bacteriol ; 195(24): 5555-66, 2013 Dec.
Article in English | MEDLINE | ID: mdl-24123814

ABSTRACT

Approximately 50% of cell wall peptidoglycan in Gram-negative bacteria is recycled with each generation. The primary substrates used for peptidoglycan biosynthesis and recycling in the cytoplasm are GlcNAc-MurNAc(anhydro)-tetrapeptide and its degradation product, the free tetrapeptide. This complex process involves ∼15 proteins, among which the cytoplasmic enzyme ld-carboxypeptidase A (LdcA) catabolizes the bond between the last two l- and d-amino acid residues in the tetrapeptide to form the tripeptide, which is then utilized as a substrate by murein peptide ligase (Mpl). LdcA has been proposed as an antibacterial target. The crystal structure of Novosphingobium aromaticivorans DSM 12444 LdcA (NaLdcA) was determined at 1.89-Šresolution. The enzyme was biochemically characterized and its interactions with the substrate modeled, identifying residues potentially involved in substrate binding. Unaccounted electron density at the dimer interface in the crystal suggested a potential site for disrupting protein-protein interactions should a dimer be required to perform its function in bacteria. Our analysis extends the identification of functional residues to several other homologs, which include enzymes from bacteria that are involved in hydrocarbon degradation and destruction of coral reefs. The NaLdcA crystal structure provides an alternate system for investigating the structure-function relationships of LdcA and increases the structural coverage of the protagonists in bacterial cell wall recycling.


Subject(s)
Carboxypeptidases/chemistry , Carboxypeptidases/metabolism , Peptidoglycan/metabolism , Sphingomonadaceae/enzymology , Amino Acid Sequence , Binding Sites , Crystallography, X-Ray , Models, Molecular , Molecular Sequence Data , Protein Binding , Protein Conformation , Protein Multimerization
8.
J Biol Chem ; 284(37): 25268-79, 2009 Sep 11.
Article in English | MEDLINE | ID: mdl-19567872

ABSTRACT

SsgA-like proteins (SALPs) are a family of homologous cell division-related proteins that occur exclusively in morphologically complex actinomycetes. We show that SsgB, a subfamily of SALPs, is the archetypal SALP that is functionally conserved in all sporulating actinomycetes. Sporulation-specific cell division of Streptomyces coelicolor ssgB mutants is restored by introduction of distant ssgB orthologues from other actinomycetes. Interestingly, the number of septa (and spores) of the complemented null mutants is dictated by the specific ssgB orthologue that is expressed. The crystal structure of the SsgB from Thermobifida fusca was determined at 2.6 A resolution and represents the first structure for this family. The structure revealed similarities to a class of eukaryotic "whirly" single-stranded DNA/RNA-binding proteins. However, the electro-negative surface of the SALPs suggests that neither SsgB nor any of the other SALPs are likely to interact with nucleotide substrates. Instead, we show that a conserved hydrophobic surface is likely to be important for SALP function and suggest that proteins are the likely binding partners.


Subject(s)
Actinobacteria/metabolism , Bacterial Proteins/chemistry , Bacterial Proteins/physiology , Amino Acid Sequence , Binding Sites , Cell Division , Cryoelectron Microscopy , Crystallography, X-Ray/methods , Escherichia coli/metabolism , Genetic Complementation Test , Microscopy, Fluorescence/methods , Microscopy, Phase-Contrast/methods , Molecular Sequence Data , Mutation , Sequence Homology, Amino Acid , Spores, Bacterial
9.
Acta Crystallogr Sect F Struct Biol Cryst Commun ; 66(Pt 10): 1153-9, 2010 Oct 01.
Article in English | MEDLINE | ID: mdl-20944205

ABSTRACT

The first structural representative of the domain of unknown function DUF2006 family, also known as Pfam family PF09410, comprises a lipocalin-like fold with domain duplication. The finding of the calycin signature in the N-terminal domain, combined with remote sequence similarity to two other protein families (PF07143 and PF08622) implicated in isoprenoid metabolism and the oxidative stress response, support an involvement in lipid metabolism. Clusters of conserved residues that interact with ligand mimetics suggest that the binding and regulation sites map to the N-terminal domain and to the interdomain interface, respectively.


Subject(s)
Bacterial Proteins/chemistry , Databases, Genetic , Lipid Metabolism , Nitrosomonas europaea/chemistry , Amino Acid Sequence , Crystallography, X-Ray , Models, Molecular , Molecular Sequence Data , Nitrosomonas europaea/metabolism , Oxidative Stress , Protein Structure, Tertiary , Sequence Alignment , Sequence Homology, Amino Acid
10.
Acta Crystallogr Sect F Struct Biol Cryst Commun ; 66(Pt 10): 1160-6, 2010 Oct 01.
Article in English | MEDLINE | ID: mdl-20944206

ABSTRACT

SSO2064 is the first structural representative of PF01796 (DUF35), a large prokaryotic family with a wide phylogenetic distribution. The structure reveals a novel two-domain architecture comprising an N-terminal, rubredoxin-like, zinc ribbon and a C-terminal, oligonucleotide/oligosaccharide-binding (OB) fold domain. Additional N-terminal helical segments may be involved in protein-protein interactions. Domain architectures, genomic context analysis and functional evidence from certain bacterial representatives of this family suggest that these proteins form a novel fatty-acid-binding component that is involved in the biosynthesis of lipids and polyketide antibiotics and that they possibly function as acyl-CoA-binding proteins. This structure has led to a re-evaluation of the DUF35 family, which has now been split into two entries in the latest Pfam release (v.24.0).


Subject(s)
Acyl Coenzyme A/chemistry , Archaeal Proteins/chemistry , Protein Folding , Sulfolobus solfataricus/chemistry , Zinc/chemistry , Amino Acid Sequence , Archaeal Proteins/genetics , Archaeal Proteins/metabolism , Crystallography, X-Ray , Genome, Archaeal , Models, Molecular , Molecular Sequence Data , Protein Binding , Protein Structure, Tertiary , Sulfolobus solfataricus/genetics , Sulfolobus solfataricus/metabolism
11.
Acta Crystallogr Sect F Struct Biol Cryst Commun ; 66(Pt 10): 1167-73, 2010 Oct 01.
Article in English | MEDLINE | ID: mdl-20944207

ABSTRACT

The crystal structure of Dhaf4260 from Desulfitobacterium hafniense DCB-2 was determined by single-wavelength anomalous diffraction (SAD) to a resolution of 2.01 Šusing the semi-automated high-throughput pipeline of the Joint Center for Structural Genomics (JCSG) as part of the NIGMS Protein Structure Initiative (PSI). This protein structure is the first representative of the PF04016 (DUF364) Pfam family and reveals a novel combination of two well known domains (an enolase N-terminal-like fold followed by a Rossmann-like domain). Structural and bioinformatic analyses reveal partial similarities to Rossmann-like methyltransferases, with residues from the enolase-like fold combining to form a unique active site that is likely to be involved in the condensation or hydrolysis of molecules implicated in the synthesis of flavins, pterins or other siderophores. The genome context of Dhaf4260 and homologs additionally supports a role in heavy-metal chelation.


Subject(s)
Bacterial Proteins/chemistry , Desulfitobacterium/chemistry , Metals, Heavy/chemistry , Phosphopyruvate Hydratase/chemistry , Protein Folding , Amino Acid Sequence , Bacterial Proteins/metabolism , Catalytic Domain , Crystallography, X-Ray , Desulfitobacterium/metabolism , Metals, Heavy/metabolism , Models, Molecular , Molecular Sequence Data , Protein Binding , Protein Structure, Tertiary
12.
Acta Crystallogr Sect F Struct Biol Cryst Commun ; 66(Pt 10): 1174-81, 2010 Oct 01.
Article in English | MEDLINE | ID: mdl-20944208

ABSTRACT

Proteins with the DUF2063 domain constitute a new Pfam family, PF09836. The crystal structure of a member of this family, NGO1945 from Neisseria gonorrhoeae, has been determined and reveals that the N-terminal DUF2063 domain is likely to be a DNA-binding domain. In conjunction with the rest of the protein, NGO1945 is likely to be involved in transcriptional regulation, which is consistent with genomic neighborhood analysis. Of the 216 currently known proteins that contain a DUF2063 domain, the most significant sequence homologs of NGO1945 (∼40-99% sequence identity) are from various Neisseria and Haemophilus species. As these are important human pathogens, NGO1945 represents an interesting candidate for further exploration via biochemical studies and possible therapeutic intervention.


Subject(s)
Bacterial Proteins/chemistry , Gene Expression Regulation , Neisseria gonorrhoeae/chemistry , Transcription, Genetic , Amino Acid Sequence , Bacterial Proteins/genetics , Crystallography, X-Ray , Genome, Bacterial , Models, Molecular , Molecular Sequence Data , Neisseria gonorrhoeae/genetics , Protein Structure, Quaternary , Protein Structure, Tertiary , Structural Homology, Protein
13.
Acta Crystallogr Sect F Struct Biol Cryst Commun ; 66(Pt 10): 1182-9, 2010 Oct 01.
Article in English | MEDLINE | ID: mdl-20944209

ABSTRACT

The crystal structures of BB2672 and SPO0826 were determined to resolutions of 1.7 and 2.1 Šby single-wavelength anomalous dispersion and multiple-wavelength anomalous dispersion, respectively, using the semi-automated high-throughput pipeline of the Joint Center for Structural Genomics (JCSG) as part of the NIGMS Protein Structure Initiative (PSI). These proteins are the first structural representatives of the PF06684 (DUF1185) Pfam family. Structural analysis revealed that both structures adopt a variant of the Bacillus chorismate mutase fold (BCM). The biological unit of both proteins is a hexamer and analysis of homologs indicates that the oligomer interface residues are highly conserved. The conformation of the critical regions for oligomerization appears to be dependent on pH or salt concentration, suggesting that this protein might be subject to environmental regulation. Structural similarities to BCM and genome-context analysis suggest a function in amino-acid synthesis.


Subject(s)
Amino Acids/metabolism , Bordetella bronchiseptica/enzymology , Chorismate Mutase/chemistry , Protein Folding , Rhodobacteraceae/enzymology , Amino Acid Sequence , Bacillus/enzymology , Chorismate Mutase/metabolism , Crystallography, X-Ray , Models, Molecular , Molecular Sequence Data , Protein Structure, Quaternary , Protein Structure, Tertiary , Structural Homology, Protein
14.
Acta Crystallogr Sect F Struct Biol Cryst Commun ; 66(Pt 10): 1198-204, 2010 Oct 01.
Article in English | MEDLINE | ID: mdl-20944211

ABSTRACT

The crystal structure of Jann_2411 from Jannaschia sp. strain CCS1, a member of the Pfam PF07336 family classified as a domain of unknown function (DUF1470), was solved to a resolution of 1.45 Šby multiple-wavelength anomalous dispersion (MAD). This protein is the first structural representative of the DUF1470 Pfam family. Structural analysis revealed a two-domain organization, with the N-terminal domain presenting a new fold called the ABATE domain that may bind an as yet unknown ligand. The C-terminal domain forms a treble-clef zinc finger that is likely to be involved in DNA binding. Analysis of the Jann_2411 protein and the broader ABATE-domain family suggests a role as stress-induced transcriptional regulators.


Subject(s)
Bacterial Proteins/chemistry , Rhodobacteraceae/chemistry , Amino Acid Sequence , Crystallography, X-Ray , Models, Molecular , Molecular Sequence Data , Protein Structure, Quaternary , Protein Structure, Tertiary , Sequence Alignment , Zinc Fingers
15.
Acta Crystallogr Sect F Struct Biol Cryst Commun ; 66(Pt 10): 1205-10, 2010 Oct 01.
Article in English | MEDLINE | ID: mdl-20944212

ABSTRACT

The structure of LP2179, a member of the PF08866 (DUF1831) family, suggests a novel α+ß fold comprising two ß-sheets packed against a single helix. A remote structural similarity to two other uncharacterized protein families specific to the Bacillus genus (PF08868 and PF08968), as well as to prokaryotic S-adenosylmethionine decarboxylases, is consistent with a role in amino-acid metabolism. Genomic neighborhood analysis of LP2179 supports this functional assignment, which might also then be extended to PF08868 and PF08968.


Subject(s)
Amino Acids/metabolism , Bacterial Proteins/chemistry , Lactobacillus plantarum/chemistry , Protein Folding , Amino Acid Sequence , Bacterial Proteins/metabolism , Crystallography, X-Ray , Lactobacillus plantarum/metabolism , Models, Molecular , Molecular Sequence Data , Protein Structure, Tertiary , Sequence Alignment , Structural Homology, Protein
16.
Acta Crystallogr Sect F Struct Biol Cryst Commun ; 66(Pt 10): 1211-7, 2010 Oct 01.
Article in English | MEDLINE | ID: mdl-20944213

ABSTRACT

The crystal structure of PA1994 from Pseudomonas aeruginosa, a member of the Pfam PF06475 family classified as a domain of unknown function (DUF1089), reveals a novel fold comprising a 15-stranded ß-sheet wrapped around a single α-helix that assembles into a tight dimeric arrangement. The remote structural similarity to lipoprotein localization factors, in addition to the presence of an acidic pocket that is conserved in DUF1089 homologs, phospholipid-binding and sugar-binding proteins, indicate a role for PA1994 and the DUF1089 family in glycolipid metabolism. Genome-context analysis lends further support to the involvement of this family of proteins in glycolipid metabolism and indicates possible activation of DUF1089 homologs under conditions of bacterial cell-wall stress or host-pathogen interactions.


Subject(s)
Bacterial Proteins/chemistry , Glycolipids/metabolism , Protein Folding , Pseudomonas aeruginosa/chemistry , Amino Acid Sequence , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Crystallography, X-Ray , Genome, Bacterial , Models, Molecular , Molecular Sequence Data , Protein Structure, Quaternary , Protein Structure, Tertiary , Pseudomonas aeruginosa/genetics , Pseudomonas aeruginosa/metabolism
17.
Acta Crystallogr Sect F Struct Biol Cryst Commun ; 66(Pt 10): 1218-25, 2010 Oct 01.
Article in English | MEDLINE | ID: mdl-20944214

ABSTRACT

The crystal structures of SPO0140 and Sbal_2486 were determined using the semiautomated high-throughput pipeline of the Joint Center for Structural Genomics (JCSG) as part of the NIGMS Protein Structure Initiative (PSI). The structures revealed a conserved core with domain duplication and a superficial similarity of the C-terminal domain to pleckstrin homology-like folds. The conservation of the domain interface indicates a potential binding site that is likely to involve a nucleotide-based ligand, with genome-context and gene-fusion analyses additionally supporting a role for this family in signal transduction, possibly during oxidative stress.


Subject(s)
Bacterial Proteins/chemistry , Protein Folding , Rhodobacteraceae/chemistry , Shewanella/chemistry , Signal Transduction , Amino Acid Sequence , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Crystallography, X-Ray , Genome, Bacterial , Models, Molecular , Molecular Sequence Data , Protein Structure, Secondary , Protein Structure, Tertiary , Rhodobacteraceae/genetics , Rhodobacteraceae/metabolism , Shewanella/genetics , Shewanella/metabolism , Structural Homology, Protein
18.
Acta Crystallogr Sect F Struct Biol Cryst Commun ; 66(Pt 10): 1237-44, 2010 Oct 01.
Article in English | MEDLINE | ID: mdl-20944217

ABSTRACT

The crystal structure of a putative NTPase, YP_001813558.1 from Exiguobacterium sibiricum 255-15 (PF09934, DUF2166) was determined to 1.78 Šresolution. YP_001813558.1 and its homologs (dimeric dUTPases, MazG proteins and HisE-encoded phosphoribosyl ATP pyrophosphohydrolases) form a superfamily of all-α-helical NTP pyrophosphatases. In dimeric dUTPase-like proteins, a central four-helix bundle forms the active site. However, in YP_001813558.1, an unexpected intertwined swapping of two of the helices that compose the conserved helix bundle results in a `linked dimer' that has not previously been observed for this family. Interestingly, despite this novel mode of dimerization, the metal-binding site for divalent cations, such as magnesium, that are essential for NTPase activity is still conserved. Furthermore, the active-site residues that are involved in sugar binding of the NTPs are also conserved when compared with other α-helical NTPases, but those that recognize the nucleotide bases are not conserved, suggesting a different substrate specificity.


Subject(s)
Bacillales/enzymology , Pyrophosphatases/chemistry , Amino Acid Sequence , Crystallography, X-Ray , Models, Molecular , Molecular Sequence Data , Protein Multimerization , Protein Structure, Quaternary , Protein Structure, Tertiary , Structural Homology, Protein
19.
Acta Crystallogr Sect F Struct Biol Cryst Commun ; 66(Pt 10): 1245-53, 2010 Oct 01.
Article in English | MEDLINE | ID: mdl-20944218

ABSTRACT

The crystal structures of the proteins encoded by the YP_749275.1 and YP_001095227.1 genes from Shewanella frigidimarina and S. loihica, respectively, have been determined at 1.8 and 2.25 Šresolution, respectively. These proteins are members of a novel family of bacterial proteins that adopt the α/ß SpoIIAA-like fold found in STAS and CRAL-TRIO domains. Despite sharing 54% sequence identity, these two proteins adopt distinct conformations arising from different dispositions of their α2 and α3 helices. In the `open' conformation (YP_001095227.1), these helices are 15 Šapart, leading to the creation of a deep nonpolar cavity. In the `closed' structure (YP_749275.1), the helices partially unfold and rearrange, occluding the cavity and decreasing the solvent-exposed hydrophobic surface. These two complementary structures are reminiscent of the conformational switch in CRAL-TRIO carriers of hydrophobic compounds. It is suggested that both proteins may associate with the lipid bilayer in their `open' monomeric state by inserting their amphiphilic helices, α2 and α3, into the lipid bilayer. These bacterial proteins may function as carriers of nonpolar substances or as interfacially activated enzymes.


Subject(s)
Bacterial Proteins/chemistry , Cell Membrane/chemistry , Shewanella/chemistry , Amino Acid Sequence , Bacterial Proteins/metabolism , Cell Membrane/metabolism , Crystallography, X-Ray , Ligands , Models, Molecular , Molecular Sequence Data , Protein Binding , Protein Structure, Quaternary , Protein Structure, Tertiary , Sequence Alignment , Sequence Homology, Amino Acid , Shewanella/metabolism , Structural Homology, Protein
20.
Acta Crystallogr Sect F Struct Biol Cryst Commun ; 66(Pt 10): 1254-60, 2010 Oct 01.
Article in English | MEDLINE | ID: mdl-20944219

ABSTRACT

KPN03535 (gi|152972051) is a putative lipoprotein of unknown function that is secreted by Klebsiella pneumoniae MGH 78578. The crystal structure reveals that despite a lack of any detectable sequence similarity to known structures, it is a novel variant of the OB-fold and structurally similar to the bacterial Cpx-pathway protein NlpE, single-stranded DNA-binding (SSB) proteins and toxins. K. pneumoniae MGH 78578 forms part of the normal human skin, mouth and gut flora and is an opportunistic pathogen that is linked to about 8% of all hospital-acquired infections in the USA. This structure provides the foundation for further investigations into this divergent member of the OB-fold family.


Subject(s)
Bacterial Proteins/chemistry , Klebsiella pneumoniae/chemistry , Lipoproteins/chemistry , Amino Acid Sequence , Crystallography, X-Ray , Models, Molecular , Molecular Sequence Data , Protein Folding , Protein Structure, Tertiary
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