Retrospective cohort evaluation of non-HIV Castleman disease from a single academic center in Beijing, China.

The purpose is to ascertain the clinical impact of Castleman disease (CD) by reassessment of the real-world data from Peking University First Hospital (PKUFH). The results will contribute to the standardization of diagnosis and treatment on CDs. Based on the last 15-year retrospective real-world data from Peking University First Hospital (PKUFH), we reclassified and re-evaluated the clinical and pathological information of patients with pathologically suspected diagnosis of CD. A total of 203 patients were included in our study, in which the diagnosis of CD was confirmed in 189 cases, including 118 patients with unicentric CD (UCD, n = 118, 62.4%) and 71 patients with multicentric CD (MCD, n = 71, 37.6%). A total of 44.1% (n = 52) of UCDs in our cohort were complicated with paraneoplastic pemphigus (PNP). The treatment of UCD is primarily surgical, with a 5-year overall survival (OS) of 88.1%. Patients with PNP had a poorer prognosis than those without PNP (82.9% (95% CI 123-178) vs 92.8% (95% CI 168-196), log-rank p = 0.041). The rate of concurrent systemic symptoms was 74.6% (n = 53), and renal involvement occurred in 49.3% (n = 35) MCD patients. The MCD treatments were mainly chemotherapy regimens, with a 5-year OS of 77.6% (95% CI, 143-213). Patients with UCD demonstrate a better overall prognosis than patients with MCD. But the prognosis of those complicated with PNP was poor. The differential diagnosis of MCD is extensive. MCD treatment in China is heterogeneous. The inaccessibility of anti-IL-6-targeted drugs in China may contribute to the poor prognosis for patients with MCD.A preprint has previously been published (Guo et al. 34).

Comparison of SB-SDS and other decellularization methods for the acellular nerve graft: Biological evaluation and nerve repair in vitro and in vivo.

We aimed to compare the performance of acellular nerves prepared by different decellularization methods, screening out the optimal decellularization protocol, repairing the sciatic nerve defects in rats by the allogeneic transplantation, and evaluating the effect of regenerative nerve on the function reconstruction. The Sondell, SB-SDS, TnBP, and the high/low permeation methods were used to decellularize donor nerves. Nerves without any treatment were as the control group. The histological results were evaluated by HE staining and toluidine blue (TB) staining. The proliferation activity of L929 cells was detected by CCK-8 assay. The adhesion of Schwann cells was observed and quantified by SEM. Balb/c mice were used to evaluate the cellular and humoral immunogenicity of the nerve scaffolds. The rat sciatic nerve defect model was applied to observe the repair effect of acellular nerve scaffold in vivo. To SB-SDS group, it remained the original state of the nerves, with no observed nucleus and axons, the neurotoxicity grade detected by CCK-8 being almost 0, and it kept the largest number of Schwann cells adhered to the acellular nerve and the better morphology. Further, it showed that the selected SB-SDS rats acellular nerve scaffold could promote the nerve repair of the rats by HE staining and TB staining. We could conclude that the acellular nerve matrix prepared by the SB-SDS method effectively removes the cellular components in the nerve tissue and retains the main components of the extracellular matrix of the nerve tissue, whose rats decellularized nerve scaffold could promote the sciatic nerve repair better.

Study on Key Technologies of Geosteering for Shale Gas Horizontal Wells in the Complex Structural Area of Block H in Western Hubei-Eastern Chongqing.

Block H, located in western Hubei-eastern Chongqing, remains at a low exploration degree. Characterized by its complex structural attributes, the area presents adverse conditions such as a thin thickness of high-quality shale reservoir, rapid lateral formation occurrence, and poor stratigraphic correlation, challenging conventional geosteering methods. The primary shale gas reservoir in Block H corresponds to the Upper Permian Wujiaping Formation. To ensure that the shale gas horizontal wells in this block effectively penetrate high-quality gas reservoirs, this study delves into the geological characteristics of this stratigraphic unit, identifies principal challenges faced by current geosteering techniques, and introduces a tailored technical solution. This solution encompasses the application of real-time 3D geological modeling to track while drilling, identification of steering marker layers, optimization of steerable tools, and optimization of the steering trajectory while drilling. In the technology of optimization of the steering trajectory while drilling, a trajectory control calculation model based on the average angle technique was established for the first time. Additionally, a sectional control chart for marker layers and well inclination under different deflecting constraints was established. These methods have solved the problems of large error in target prediction and poor trajectory control effects by using the equal thickness method alone. The findings from this study can significantly enhance target prediction and trajectory control accuracy in complex structural areas, offering pivotal insights for the proficient development of analogous shale gas reservoirs in the future.

Refracturing shale gas wells in China: Doubling water consumption for enhanced gas recovery.

Shale gas extraction in China often faces inadequate reservoir stimulation after initial fracturing of the wells, leading to production challenges despite abundant residual gas. Refracturing is an effective approach to enhance gas recovery; however, its impact on water consumption remains understudied. This study analyzes two refracturing techniques employed in China's largest shale production field, Fuling: temporary plugging and diverting refracturing (TPD) and wellbore reconstruction refracturing (WR), focusing on fracturing efficiency and water consumption. The results demonstrate that WR refracturing exhibits superior fracturing performance but consumes 1.3 times more water than initial fracturing. Considering 315 wells that required refracturing from 2013 to 2017, this study reveals, for the first time, that the lifecycle water consumption for shale gas production with refracturing is more than twice that without refracturing. The estimated total water consumption for the Fuling shale gas field over the next decade, incorporating refracturing, is approximately 7594.53 × 104 m3. By including the water consumption of refracturing, this study provides a more comprehensive evaluation of water usage throughout the entire lifecycle of shale gas development. The findings offer new insights for assessing water consumption in global shale gas development and highlight the importance of considering refracturing when evaluating the environmental impacts and resource management strategies associated with shale gas extraction.

Efficacy of daratumumab on multiple myeloma patients with renal insufficiency: a systematic review and meta-analysis.

BACKGROUND: Renal insufficiency (RI) is a key factor affecting the prognosis of multiple myeloma (MM) patients. Because the benefit of daratumumab for treating MM patients with RI remains unclear, our objective was to evaluate the efficacy of daratumumab on MM patients with RI. METHODS: We conducted a systematic search of the PubMed, EMBASE, and Cochrane Library databases as of October 24, 2023. Two independent reviewers screened the article titles, abstracts, and full text to identify the randomized controlled trials (RCTs) meeting the inclusion and exclusion criteria. Meta-analyses were performed using RevMan version 5.4. Outcomes of interest were progression-free survival (PFS), overall survival (OS), complete response or better (≥CR), and minimal residual disease (MRD) negativity, all calculated as hazard ratios (HRs) or risk ratios (RRs) with 95% confidence intervals (CIs). RESULTS: A total of 10 RCTs with 5003 patients were included. Add-on daratumumab improved PFS and OS among newly diagnosed MM (NDMM) patients with RI (HR 0.48 [95% CI: 0.36, 0.64, I2 = 65%] and HR 0.63 [95% CI: 0.48, 0.82, I2 = 0%]) as well as relapsed/refractory MM (RRMM)-RI patients, compared with the control group (HR 0.46 [95% CI: 0.37, 0.58, I2 = 0%] and HR 0.68 [95% CI: 0.51, 0.92, I2 = 0%]). In terms of the renal status, the efficacy of add-on daratumumab for MMRI patients was similar to that for MM patients with normal renal function. A prolonged PFS benefit for add-on daratumumab treatment versus the control was evident across all RRMM-RI subgroups, and the benefits tended to increase with the follow-up time. CONCLUSIONS: Our results indicate that MM patients with RI could benefit from a daratumumab-added regimen regardless of MM status. Additional high-quality RCTs are still warranted to confirm our findings.

Changes in antioxidant system and sucrose metabolism in maize varieties exposed to Cd.

Different maize varieties respond differentially to cadmium (Cd) stress. However, the physiological mechanisms that determine the response are not well defined. Antioxidant systems and sucrose metabolism help plants to cope with abiotic stresses, including Cd stress. The relationship of these two systems in the response to Cd stress is unclear. Seed is sensitive to Cd stress during germination. In this study, we investigated changes in the antioxidant system, sucrose metabolism, and abscisic acid and gibberellin concentrations in two maize varieties with low (FY9) or high (SY33) sensitivities to Cd under exposure to CdCl2 (20 mg L-1) at different stages of germination (3, 6, and 9 days).The seed germination and seedling growth were inhibited under Cd stress. The superoxide, malondialdehyde, and proline concentrations, and the superoxide dismutase, peroxidase, catalase, and lipoxygenase activities increased compared with those of the control (CK; without Cd). The expression levels of three genes (ZmOPR2, ZmOPR5, and ZmPP2C6) responsive to oxidative stress increased differentially in the two varieties under Cd stress. The activity of the antioxidant system and the transcript levels of oxidative stress-responsive genes were higher in the Cd-tolerant variety, FY9, than in the sensitive variety, SY33. Sucrose metabolism was increased under Cd stress compared with that of the CK and was more active in the Cd-sensitive variety, SY33. These results suggest that the antioxidant system is the first response to Cd stress in maize, and that sucrose metabolism is cooperative and complementary under exposure to Cd.

Corrigendum: Case report: Primary aldosteronism and subclinical cushing syndrome in a 49-year-old woman with hypertension plus hypokalaemia.

[This corrects the article DOI: 10.3389/fcvm.2022.911333.].

Case Report: Primary Aldosteronism and Subclinical Cushing Syndrome in a 49-Year-Old Woman With Hypertension Plus Hypokalaemia.

Background: Coexisting primary aldosteronism (PA) and subclinical Cushing's syndrome (SCS) caused by bilateral adrenocortical adenomas have occasionally been reported. Precise diagnosis and treatment of the disease pose a challenge to clinicians due to its atypical clinical manifestations and laboratory findings. Case Summary: A 49-year-old woman was admitted to our hospital due to fatigue, increased nocturia and refractory hypertension. The patient had a history of severe left hydronephrosis 6 months prior. Laboratory examinations showed hypokalaemia (2.58 mmol/L) and high urine potassium (71 mmol/24 h). Adrenal computed tomography (CT) showed bilateral adrenal masses. Undetectable ACTH and unsuppressed plasma cortisol levels by dexamethasone indicated ACTH-independent Cushing's syndrome. Although the upright aldosterone-to-renin ratio (ARR) was 3.06 which did not exceed 3.7, elevated plasma aldosterone concentrations (PAC) with unsuppressed PAC after the captopril test still suggested PA. Adrenal venous sampling (AVS) without adrenocorticotropic hormone further revealed hypersecretion of aldosterone from the right side and no dominant side of cortisol secretion. A laparoscopic right adrenal tumor resection was performed. The pathological diagnosis was adrenocortical adenoma. After the operation, the supine and standing PAC were normalized; while the plasma cortisol levels postoperatively were still high and plasma renin was activated. The patient's postoperative serum potassium and 24-h urine potassium returned to normal without any pharmacological treatment. In addition, the patient's blood pressure was controlled normally with irbesartan alone. Conclusion: Patients with refractory hypertension should be screened for the cause of secondary hypertension. AVS should be performed in patients in which PA is highly suspected to determine whether there is the option of surgical treatment. Moreover, patients with PA should be screened for hypercortisolism, which can contribute to a proper understanding of the AVS result.

2-[2-(2-Carb-oxy-phen-yl)hydrazinyl-idene]-3-oxo-N-phenyl-butyramide.

In the title compound, C(17)H(15)N(3)O(4), the mol-ecule is in the keto-hydrazone form. Intra-molecular N-H⋯O hydrogen bonds ensure that the mol-ecule is nearly planar (r.m.s. deviation of non-H atoms is 0.098 Å), with the two benzene rings forming a dihedral angle of 10.04 (2)°. In the crystal, inversion dimers are formed via pairs of O-H⋯O hydrogen bonds involving the -CO(2)H groups.

Effect of water motion and microhabitat preferences on spatio-temporal variation of epiphytic communities: a case study in an artificial rocky reef system, Laoshan Bay, China.

Epiphytic fauna community structure is mainly determined by macro-scale environmental characteristics (e.g., water motion), whereas the influences of, and interaction with, micro-scale habitat-forming species are not well known. In order to explore the effects of water motion and functional macroalgal group on the community structure of epiphytic faunas, macroalgae and epiphytic faunas from four isolated artificial rocky reefs with different levels of hydrodynamic conditions were investigated in Laoshan Bay of China. A total of 32 macroalgal species and 22 faunal species were recorded on the surveys. The biomass and abundance of macroalgae and epiphytic faunas showed significant variations among stations and months. The biomass and abundance of epiphytic faunas were highest at outer Huangshankou station (H1) with medium hydrodynamic force. The lowest biomass of epiphytic faunas occurred at inner Huangshankou station (H3) with highest level of water motion, while the lowest abundance of epiphytic faunas occurred at Liepo station (L) with lowest level of water motion. Results showed that medium water motion level contributed to increasing biodiversity of epiphytic faunas. Epiphytic communities generally presented higher biomass and abundance in summer than in spring or autumn, which indicated temperature could be the ecological driver for temporal variation of epiphytic communities. The epiphytic faunas showed distinct preferences to structural morphology of macroalgae which forms suitable habitat for these organisms. This study suggests that artificial rocky reefs are of great significance to restore and maintain coastal biodiversity, and appropriate physical disturbance promote community reestablishment.

Conversion from long-term cultivated wheat field to Jerusalem artichoke plantation changed soil fungal communities.

Understanding soil microbial communities in agroecosystems has the potential to contribute to the improvement of agricultural productivity and sustainability. Effects of conversion from long-term wheat plantation to Jerusalem artichoke (JA) plantation on soil fungal communities were determined by amplicon sequencing of total fungal ITS regions. Quantitative PCR and PCR-denaturing gradient gel electrophoresis were also used to analyze total fungal and Trichoderma spp. ITS regions and Fusarium spp. Ef1α genes. Results showed that soil organic carbon was higher in the first cropping of JA and Olsen P was lower in the third cropping of JA. Plantation conversion changed soil total fungal and Fusarium but not Trichoderma spp. community structures and compositions. The third cropping of JA had the lowest total fungal community diversity and Fusarium spp. community abundance, but had the highest total fungal and Trichoderma spp. community abundances. The relative abundances of potential fungal pathogens of wheat were higher in the wheat field. Fungal taxa with plant growth promoting, plant pathogen or insect antagonistic potentials were enriched in the first and second cropping of JA. Overall, short-term conversion from wheat to JA plantation changed soil fungal communities, which is related to changes in soil organic carbon and Olsen P contents.