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1.
Cell ; 164(1-2): 219-232, 2016 Jan 14.
Article in English | MEDLINE | ID: mdl-26771493

ABSTRACT

Although a number of repair strategies have been shown to promote axon outgrowth following neuronal injury in the mammalian CNS, it remains unclear whether regenerated axons establish functional synapses and support behavior. Here, in both juvenile and adult mice, we show that either PTEN and SOCS3 co-deletion, or co-overexpression of osteopontin (OPN)/insulin-like growth factor 1 (IGF1)/ciliary neurotrophic factor (CNTF), induces regrowth of retinal axons and formation of functional synapses in the superior colliculus (SC) but not significant recovery of visual function. Further analyses suggest that regenerated axons fail to conduct action potentials from the eye to the SC due to lack of myelination. Consistent with this idea, administration of voltage-gated potassium channel blockers restores conduction and results in increased visual acuity. Thus, enhancing both regeneration and conduction effectively improves function after retinal axon injury.


Subject(s)
Axons/physiology , Superior Colliculi/physiology , 4-Aminopyridine/pharmacology , Animals , Axons/drug effects , Ciliary Neurotrophic Factor/metabolism , Electrophysiological Phenomena , Eye/metabolism , Insulin-Like Growth Factor I/metabolism , Mice , Myelin Sheath/metabolism , Optic Nerve , Osteopontin/metabolism , PTEN Phosphohydrolase/metabolism , Potassium Channel Blockers/pharmacology , Regeneration/drug effects , Suppressor of Cytokine Signaling 3 Protein , Suppressor of Cytokine Signaling Proteins/metabolism , Synapses
2.
Cell ; 156(1-2): 17-9, 2014 Jan 16.
Article in English | MEDLINE | ID: mdl-24439367

ABSTRACT

Experience shapes brain function throughout life to varying degrees. In a recent issue of Nature, Donato et al. identify reversible shifts in focal parvalbumin cell state during adult learning, placing it on a mechanistic continuum with developmental critical periods. A disinhibitory microcircuit controls the plasticity switch to modulate memory formation.


Subject(s)
Hippocampus/cytology , Hippocampus/physiology , Interneurons/metabolism , Learning/physiology , Neuronal Plasticity/physiology , Parvalbumins/metabolism , Animals , Male
3.
Proc Natl Acad Sci U S A ; 120(2): e2123182120, 2023 01 10.
Article in English | MEDLINE | ID: mdl-36598942

ABSTRACT

Early-life experience enduringly sculpts thalamocortical (TC) axons and sensory processing. Here, we identify the very first synaptic targets that initiate critical period plasticity, heralded by altered cortical oscillations. Monocular deprivation (MD) acutely induced a transient (<3 h) peak in EEG γ-power (~40 Hz) specifically within the visual cortex, but only when the critical period was open (juvenile mice or adults after dark-rearing, Lynx1-deletion, or diazepam-rescued GAD65-deficiency). Rapid TC input loss onto parvalbumin-expressing (PV) inhibitory interneurons (but not onto nearby pyramidal cells) was observed within hours of MD in a TC slice preserving the visual pathway - again once critical periods opened. Computational TC modeling of the emergent γ-rhythm in response to MD delineated a cortical interneuronal gamma (ING) rhythm in networks of PV-cells bearing gap junctions at the start of the critical period. The ING rhythm effectively dissociated thalamic input from cortical spiking, leading to rapid loss of previously strong TC-to-PV connections through standard spike-timing-dependent plasticity rules. As a consequence, previously silent TC-to-PV connections could strengthen on a slower timescale, capturing the gradually increasing γ-frequency and eventual fade-out over time. Thus, ING enables cortical dynamics to transition from being dominated by the strongest TC input to one that senses the statistics of population TC input after MD. Taken together, our findings reveal the initial synaptic events underlying critical period plasticity and suggest that the fleeting ING accompanying a brief sensory perturbation may serve as a robust readout of TC network state with which to probe developmental trajectories.


Subject(s)
Gamma Rhythm , Interneurons , Mice , Animals , Gamma Rhythm/physiology , Interneurons/physiology , Pyramidal Cells/physiology , Gap Junctions , Parvalbumins , Neuronal Plasticity/physiology
4.
Cereb Cortex ; 33(7): 4070-4084, 2023 03 21.
Article in English | MEDLINE | ID: mdl-36130098

ABSTRACT

Traumatic brain injury (TBI) increases cerebral reactive oxygen species production, which leads to continuing secondary neuronal injury after the initial insult. Cortical parvalbumin-positive interneurons (PVIs; neurons responsible for maintaining cortical inhibitory tone) are particularly vulnerable to oxidative stress and are thus disproportionately affected by TBI. Systemic N-acetylcysteine (NAC) treatment may restore cerebral glutathione equilibrium, thus preventing post-traumatic cortical PVI loss. We therefore tested whether weeks-long post-traumatic NAC treatment mitigates cortical oxidative stress, and whether such treatment preserves PVI counts and related markers of PVI integrity and prevents pathologic electroencephalographic (EEG) changes, 3 and 6 weeks after fluid percussion injury in rats. We find that moderate TBI results in persistent oxidative stress for at least 6 weeks after injury and leads to the loss of PVIs and the perineuronal net (PNN) that surrounds them as well as of per-cell parvalbumin expression. Prolonged post-TBI NAC treatment normalizes the cortical redox state, mitigates PVI and PNN loss, and - in surviving PVIs - increases per-cell parvalbumin expression. NAC treatment also preserves normal spectral EEG measures after TBI. We cautiously conclude that weeks-long NAC treatment after TBI may be a practical and well-tolerated treatment strategy to preserve cortical inhibitory tone post-TBI.


Subject(s)
Acetylcysteine , Brain Injuries, Traumatic , Rats , Animals , Acetylcysteine/pharmacology , Acetylcysteine/metabolism , Parvalbumins/metabolism , Brain Injuries, Traumatic/metabolism , Oxidative Stress/physiology , Interneurons/metabolism
5.
Neural Comput ; : 1-33, 2023 Jun 20.
Article in English | MEDLINE | ID: mdl-37432864

ABSTRACT

We examine the efficiency of information processing in a balanced excitatory and inhibitory (E-I) network during the developmental critical period, when network plasticity is heightened. A multimodule network composed of E-I neurons was defined, and its dynamics were examined by regulating the balance between their activities. When adjusting E-I activity, both transitive chaotic synchronization with a high Lyapunov dimension and conventional chaos with a low Lyapunov dimension were found. In between, the edge of high-dimensional chaos was observed. To quantify the efficiency of information processing, we applied a short-term memory task in reservoir computing to the dynamics of our network. We found that memory capacity was maximized when optimal E-I balance was realized, underscoring both its vital role and vulnerability during critical periods of brain development.

6.
Cell ; 134(3): 508-20, 2008 Aug 08.
Article in English | MEDLINE | ID: mdl-18692473

ABSTRACT

Neural circuits are shaped by experience in early postnatal life. Distinct GABAergic connections within visual cortex determine the timing of the critical period for rewiring ocular dominance to establish visual acuity. We find that maturation of the parvalbumin (PV)-cell network that controls plasticity onset is regulated by a selective re-expression of the embryonic Otx2 homeoprotein. Visual experience promoted the accumulation of non-cell-autonomous Otx2 in PV-cells, and cortical infusion of exogenous Otx2 accelerated both PV-cell development and critical period timing. Conversely, conditional removal of Otx2 from non-PV cells or from the visual pathway abolished plasticity. Thus, the experience-dependent transfer of a homeoprotein may establish the physiological milieu for postnatal plasticity of a neural circuit.


Subject(s)
Neuronal Plasticity , Otx Transcription Factors/metabolism , Visual Cortex/physiology , Animals , Humans , Interneurons/physiology , Mice , Otx Transcription Factors/genetics , Parvalbumins/metabolism , Recombinant Proteins/genetics , Recombinant Proteins/metabolism , Sensory Deprivation , Visual Pathways
7.
Proc Natl Acad Sci U S A ; 117(38): 23298-23303, 2020 09 22.
Article in English | MEDLINE | ID: mdl-31332003

ABSTRACT

Neurodevelopmental spectrum disorders like autism (ASD) are diagnosed, on average, beyond age 4 y, after multiple critical periods of brain development close and behavioral intervention becomes less effective. This raises the urgent need for quantitative, noninvasive, and translational biomarkers for their early detection and tracking. We found that both idiopathic (BTBR) and genetic (CDKL5- and MeCP2-deficient) mouse models of ASD display an early, impaired cholinergic neuromodulation as reflected in altered spontaneous pupil fluctuations. Abnormalities were already present before the onset of symptoms and were rescued by the selective expression of MeCP2 in cholinergic circuits. Hence, we trained a neural network (ConvNetACh) to recognize, with 97% accuracy, patterns of these arousal fluctuations in mice with enhanced cholinergic sensitivity (LYNX1-deficient). ConvNetACh then successfully detected impairments in all ASD mouse models tested except in MeCP2-rescued mice. By retraining only the last layers of ConvNetACh with heart rate variation data (a similar proxy of arousal) directly from Rett syndrome patients, we generated ConvNetPatients, a neural network capable of distinguishing them from typically developing subjects. Even with small cohorts of rare patients, our approach exhibited significant accuracy before (80% in the first and second year of life) and into regression (88% in stage III patients). Thus, transfer learning across species and modalities establishes spontaneous arousal fluctuations combined with deep learning as a robust noninvasive, quantitative, and sensitive translational biomarker for the rapid and early detection of neurodevelopmental disorders before major symptom onset.


Subject(s)
Acetylcholine/metabolism , Arousal , Autistic Disorder/psychology , Deep Learning , Animals , Autistic Disorder/genetics , Autistic Disorder/metabolism , Autistic Disorder/physiopathology , Cohort Studies , Disease Models, Animal , Female , Humans , Male , Methyl-CpG-Binding Protein 2/genetics , Methyl-CpG-Binding Protein 2/metabolism , Mice , Protein Serine-Threonine Kinases/genetics , Protein Serine-Threonine Kinases/metabolism , Pupil/physiology , Rett Syndrome/genetics , Rett Syndrome/metabolism , Rett Syndrome/physiopathology , Rett Syndrome/psychology
8.
Proc Natl Acad Sci U S A ; 117(21): 11744-11752, 2020 05 26.
Article in English | MEDLINE | ID: mdl-32404418

ABSTRACT

Auditory experience drives neural circuit refinement during windows of heightened brain plasticity, but little is known about the genetic regulation of this developmental process. The primary auditory cortex (A1) of mice exhibits a critical period for thalamocortical connectivity between postnatal days P12 and P15, during which tone exposure alters the tonotopic topography of A1. We hypothesized that a coordinated, multicellular transcriptional program governs this window for patterning of the auditory cortex. To generate a robust multicellular map of gene expression, we performed droplet-based, single-nucleus RNA sequencing (snRNA-seq) of A1 across three developmental time points (P10, P15, and P20) spanning the tonotopic critical period. We also tone-reared mice (7 kHz pips) during the 3-d critical period and collected A1 at P15 and P20. We identified and profiled both neuronal (glutamatergic and GABAergic) and nonneuronal (oligodendrocytes, microglia, astrocytes, and endothelial) cell types. By comparing normal- and tone-reared mice, we found hundreds of genes across cell types showing altered expression as a result of sensory manipulation during the critical period. Functional voltage-sensitive dye imaging confirmed GABA circuit function determines critical period onset, while Nogo receptor signaling is required for its closure. We further uncovered previously unknown effects of developmental tone exposure on trajectories of gene expression in interneurons, as well as candidate genes that might execute tonotopic plasticity. Our single-nucleus transcriptomic resource of developing auditory cortex is thus a powerful discovery platform with which to identify mediators of tonotopic plasticity.


Subject(s)
Auditory Cortex , Cell Nucleus/metabolism , RNA , Single-Cell Analysis/methods , Transcriptome/genetics , Animals , Auditory Cortex/growth & development , Auditory Cortex/metabolism , Gene Expression Regulation, Developmental/genetics , Glutamate Decarboxylase/genetics , Glutamate Decarboxylase/metabolism , Mice , Nogo Receptors/genetics , Nogo Receptors/metabolism , RNA/analysis , RNA/genetics , RNA/metabolism , Sequence Analysis, RNA/methods
9.
Proc Natl Acad Sci U S A ; 117(38): 23242-23251, 2020 09 22.
Article in English | MEDLINE | ID: mdl-32503914

ABSTRACT

Brain plasticity is dynamically regulated across the life span, peaking during windows of early life. Typically assessed in the physiological range of milliseconds (real time), these trajectories are also influenced on the longer timescales of developmental time (nurture) and evolutionary time (nature), which shape neural architectures that support plasticity. Properly sequenced critical periods of circuit refinement build up complex cognitive functions, such as language, from more primary modalities. Here, we consider recent progress in the biological basis of critical periods as a unifying rubric for understanding plasticity across multiple timescales. Notably, the maturation of parvalbumin-positive (PV) inhibitory neurons is pivotal. These fast-spiking cells generate gamma oscillations associated with critical period plasticity, are sensitive to circadian gene manipulation, emerge at different rates across brain regions, acquire perineuronal nets with age, and may be influenced by epigenetic factors over generations. These features provide further novel insight into the impact of early adversity and neurodevelopmental risk factors for mental disorders.


Subject(s)
Brain/physiology , Neuronal Plasticity , Animals , Brain/growth & development , Circadian Clocks , Humans , Neurons/physiology , Parvalbumins/genetics , Parvalbumins/metabolism , Time Factors
10.
Proc Natl Acad Sci U S A ; 117(18): 10089-10096, 2020 05 05.
Article in English | MEDLINE | ID: mdl-32321833

ABSTRACT

Synesthesia is a neurologic trait in which specific inducers, such as sounds, automatically elicit additional idiosyncratic percepts, such as color (thus "colored hearing"). One explanation for this trait-and the one tested here-is that synesthesia results from unusually weak pruning of cortical synaptic hyperconnectivity during early perceptual development. We tested the prediction from this hypothesis that synesthetes would be superior at making discriminations from nonnative categories that are normally weakened by experience-dependent pruning during a critical period early in development-namely, discrimination among nonnative phonemes (Hindi retroflex /d̪a/ and dental /ɖa/), among chimpanzee faces, and among inverted human faces. Like the superiority of 6-mo-old infants over older infants, the synesthetic groups were significantly better than control groups at making all the nonnative discriminations across five samples and three testing sites. The consistent superiority of the synesthetic groups in making discriminations that are normally eliminated during infancy suggests that residual cortical connectivity in synesthesia supports changes in perception that extend beyond the specific synesthetic percepts, consistent with the incomplete pruning hypothesis.


Subject(s)
Cognition/physiology , Neuroimaging , Pattern Recognition, Visual/physiology , Synesthesia/diagnostic imaging , Adult , Face/diagnostic imaging , Face/physiology , Female , Humans , Male , Photic Stimulation , Synesthesia/physiopathology
11.
Proc Natl Acad Sci U S A ; 116(14): 7071-7076, 2019 04 02.
Article in English | MEDLINE | ID: mdl-30890637

ABSTRACT

Parvalbumin-positive (PV+) interneurons play a pivotal role in orchestrating windows of experience-dependent brain plasticity during development. Critical period closure is marked by the condensation of a perineuronal net (PNN) tightly enwrapping subsets of PV+ neurons, both acting as a molecular brake on plasticity and maintaining mature PV+ cell signaling. As much of the molecular organization of PNNs exists at length scales near or below the diffraction limit of light microscopy, we developed a superresolution imaging and analysis platform to visualize the structural organization of PNNs and the synaptic inputs perforating them in primary visual cortex. We identified a structural trajectory of PNN maturation featuring a range of net structures, which was accompanied by an increase in Synaptotagmin-2 (Syt2) signals on PV+ cells suggestive of increased inhibitory input between PV+ neurons. The same structural trajectory was followed by PNNs both during normal development and under conditions of critical period delay by total sensory deprivation or critical period acceleration by deletion of MeCP2, the causative gene for Rett syndrome, despite shifted maturation levels under these perturbations. Notably, superresolution imaging further revealed a decrease in Syt2 signals alongside an increase in vesicular glutamate transporter-2 signals on PV+ cells in MeCP2-deficient animals, suggesting weaker recurrent inhibitory input between PV+ neurons and stronger thalamocortical excitatory inputs onto PV+ cells. These results imply a latent imbalanced circuit signature that might promote cortical silencing in Rett syndrome before the functional regression of vision.


Subject(s)
Methyl-CpG-Binding Protein 2/metabolism , Nerve Net/metabolism , Neuronal Plasticity , Rett Syndrome/metabolism , Synapses/metabolism , Synaptotagmin II/metabolism , Visual Cortex/metabolism , Animals , Male , Methyl-CpG-Binding Protein 2/genetics , Mice , Nerve Net/diagnostic imaging , Rett Syndrome/diagnostic imaging , Rett Syndrome/genetics , Synapses/genetics , Synaptotagmin II/genetics , Visual Cortex/diagnostic imaging
12.
Cereb Cortex ; 30(12): 6108-6120, 2020 11 03.
Article in English | MEDLINE | ID: mdl-32676666

ABSTRACT

Chronic symptoms indicating excess cortical excitability follow mild traumatic brain injury, particularly repetitive mild traumatic brain injury (rmTBI). Yet mechanisms underlying post-traumatic excitation/inhibition (E/I) ratio abnormalities may differ between the early and late post-traumatic phases. We therefore measured seizure threshold and cortical gamma-aminobutyric acid (GABA) and glutamate (Glu) concentrations, 1 and 6 weeks after rmTBI in mice. We also analyzed the structure of parvalbumin-positive interneurons (PVIs), their perineuronal nets (PNNs), and their electroencephalography (EEG) signature (gamma frequency band power). For mechanistic insight, we measured cortical oxidative stress, reflected in the reduced/oxidized glutathione (GSH/GSSG) ratio. We found that seizure susceptibility increased both early and late after rmTBI. However, whereas increased Glu dominated the E/I 1 week after rmTBI, Glu concentration normalized and the E/I was instead characterized by depressed GABA, reduced per-PVI parvalbumin expression, and reduced gamma EEG power at the 6-week post-rmTBI time point. Oxidative stress was increased early after rmTBI, where transient PNN degradation was noted, and progressed throughout the monitoring period. We conclude that GSH depletion, perhaps triggered by early Glu-mediated excitotoxicity, leads to late post-rmTBI loss of PVI-dependent cortical inhibitory tone. We thus propose dampening of Glu signaling, maintenance of redox state, and preservation of PVI inhibitory capacity as therapeutic targets for post-rmTBI treatment.


Subject(s)
Brain Concussion/complications , Brain/physiopathology , Glutamic Acid/metabolism , Interneurons/physiology , Oxidative Stress , Seizures/physiopathology , gamma-Aminobutyric Acid/metabolism , Animals , Brain/metabolism , Gamma Rhythm , Male , Mice, Inbred C57BL , Parvalbumins/analysis , Seizures/etiology , Seizures/metabolism
13.
J Neurosci ; 39(25): 4847-4863, 2019 06 19.
Article in English | MEDLINE | ID: mdl-30926746

ABSTRACT

The glutamate transporter GLT-1 is highly expressed in astrocytes but also in neurons, primarily in axon terminals. We generated a conditional neuronal GLT-1 KO using synapsin 1-Cre (synGLT-1 KO) to elucidate the metabolic functions of GLT-1 expressed in neurons, here focusing on the cerebral cortex. Both synaptosomal uptake studies and electron microscopic immunocytochemistry demonstrated knockdown of GLT-1 in the cerebral cortex in the synGLT-1 KO mice. Aspartate content was significantly reduced in cerebral cortical extracts as well as synaptosomes from cerebral cortex of synGLT-1 KO compared with control littermates. 13C-Labeling of tricarboxylic acid cycle intermediates originating from metabolism of [U-13C]-glutamate was significantly reduced in synGLT-1 KO synaptosomes. The decreased aspartate content was due to diminished entry of glutamate into the tricarboxylic acid cycle. Pyruvate recycling, a pathway necessary for full glutamate oxidation, was also decreased. ATP production was significantly increased, despite unaltered oxygen consumption, in isolated mitochondria from the synGLT-1 KO. The density of mitochondria in axon terminals and perisynaptic astrocytes was increased in the synGLT-1 KO. Intramitochondrial cristae density of synGLT-1 KO mice was increased, suggesting increased mitochondrial efficiency, perhaps in compensation for reduced access to glutamate. SynGLT-1 KO synaptosomes exhibited an elevated oxygen consumption rate when stimulated with veratridine, despite a lower baseline oxygen consumption rate in the presence of glucose. GLT-1 expressed in neurons appears to be required to provide glutamate to synaptic mitochondria and is linked to neuronal energy metabolism and mitochondrial function.SIGNIFICANCE STATEMENT All synaptic transmitters need to be cleared from the extracellular space after release, and transporters are used to clear glutamate released from excitatory synapses. GLT-1 is the major glutamate transporter, and most GLT-1 is expressed in astrocytes. Only 5%-10% is expressed in neurons, primarily in axon terminals. The function of GLT-1 in axon terminals remains unknown. Here, we used a conditional KO approach to investigate the significance of the expression of GLT-1 in neurons. We found multiple abnormalities of mitochondrial function, suggesting impairment of glutamate utilization by synaptic mitochondria in the neuronal GLT-1 KO. These data suggest that GLT-1 expressed in axon terminals may be important in maintaining energy metabolism and biosynthetic activities mediated by presynaptic mitochondria.


Subject(s)
Excitatory Amino Acid Transporter 2/metabolism , Glutamic Acid/metabolism , Homeostasis/physiology , Mitochondria/metabolism , Neurons/metabolism , Synapses/metabolism , Animals , Aspartic Acid/metabolism , Cerebral Cortex/metabolism , Excitatory Amino Acid Transporter 2/genetics , Mice , Mice, Knockout , Mitochondria/genetics , Oxygen Consumption/physiology , Presynaptic Terminals/metabolism , Synapses/genetics , Synaptosomes/metabolism
14.
Neurobiol Dis ; 141: 104954, 2020 07.
Article in English | MEDLINE | ID: mdl-32445790

ABSTRACT

Dravet syndrome is a severe infantile-onset epileptic encephalopathy which begins with febrile seizures and is caused by heterozygous loss-of-function mutations of the voltage-gated sodium channel gene SCN1A. We designed a CRISPR-based gene therapy for Scn1a-haplodeficient mice using multiple guide RNAs (gRNAs) in the promoter regions together with the nuclease-deficient Cas9 fused to transcription activators (dCas9-VPR) to trigger the transcription of SCN1A or Scn1a in vitro. We tested the effect of this strategy in vivo using an adeno-associated virus (AAV) mediated system targeting inhibitory neurons and investigating febrile seizures and behavioral parameters. In both the human and mouse genes multiple guide RNAs (gRNAs) in the upstream, rather than downstream, promoter region showed high and synergistic activities to increase the transcription of SCN1A or Scn1a in cultured cells. Intravenous injections of AAV particles containing the optimal combination of 4 gRNAs into transgenic mice with Scn1a-haplodeficiency and inhibitory neuron-specific expression of dCas9-VPR at four weeks of age increased Nav1.1 expression in parvalbumin-positive GABAergic neurons, ameliorated their febrile seizures and improved their behavioral impairments. Although the usage of transgenic mice and rather modest improvements in seizures and abnormal behaviors hamper direct clinical application, our results indicate that the upregulation of Scn1a expression in the inhibitory neurons can significantly improve the phenotypes, even when applied after the juvenile stages. Our findings also suggest that the decrease in Nav1.1 is directly involved in the symptoms seen in adults with Dravet syndrome and open a way to improve this condition.


Subject(s)
Epilepsies, Myoclonic/genetics , Epilepsies, Myoclonic/physiopathology , Epilepsy/genetics , Epilepsy/physiopathology , NAV1.1 Voltage-Gated Sodium Channel/genetics , NAV1.1 Voltage-Gated Sodium Channel/physiology , Neurons/physiology , Animals , Behavior, Animal , CRISPR-Cas Systems , Disease Models, Animal , Epilepsies, Myoclonic/prevention & control , Epilepsy/prevention & control , Female , GABAergic Neurons/physiology , Genetic Therapy/methods , HEK293 Cells , Humans , Male , Mice, Inbred C57BL , Mice, Transgenic , Phenotype
15.
Bioinformatics ; 35(18): 3544-3546, 2019 09 15.
Article in English | MEDLINE | ID: mdl-30715234

ABSTRACT

SUMMARY: This note describes nTracer, an ImageJ plug-in for user-guided, semi-automated tracing of multispectral fluorescent tissue samples. This approach allows for rapid and accurate reconstruction of whole cell morphology of large neuronal populations in densely labeled brains. AVAILABILITY AND IMPLEMENTATION: nTracer was written as a plug-in for the open source image processing software ImageJ. The software, instructional documentation, tutorial videos, sample image and sample tracing results are available at https://www.cai-lab.org/ntracer-tutorial. SUPPLEMENTARY INFORMATION: Supplementary data are available at Bioinformatics online.


Subject(s)
Software , Animals , Brain , Documentation , Image Processing, Computer-Assisted , Mice , Neurons
16.
Mol Psychiatry ; 24(6): 828-838, 2019 06.
Article in English | MEDLINE | ID: mdl-30696941

ABSTRACT

Ketamine has emerged as a widespread treatment for a variety of psychiatric disorders when used at sub-anesthetic doses, but the neural mechanisms underlying its acute action remain unclear. Here, we identified NMDA receptors containing the 2A subunit (GluN2A) on parvalbumin (PV)-expressing inhibitory interneurons as a pivotal target of low-dose ketamine. Genetically deleting GluN2A receptors globally or selectively from PV interneurons abolished the rapid enhancement of visual cortical responses and gamma-band oscillations by ketamine. Moreover, during the follicular phase of the estrous cycle in female mice, the ketamine response was transiently attenuated along with a concomitant decrease of grin2A mRNA expression within PV interneurons. Thus, GluN2A receptors on PV interneurons mediate the immediate actions of low-dose ketamine treatment, and fluctuations in receptor expression across the estrous cycle may underlie sex-differences in drug efficacy.


Subject(s)
Ketamine/metabolism , Ketamine/pharmacology , Receptors, N-Methyl-D-Aspartate/physiology , Animals , Estrous Cycle/drug effects , Female , Interneurons/metabolism , Interneurons/physiology , Male , Mice , Mice, Inbred C57BL , N-Methylaspartate/metabolism , Parvalbumins/metabolism , Prefrontal Cortex/metabolism , Receptors, GABA-A/metabolism , Receptors, N-Methyl-D-Aspartate/metabolism , Sex Factors
17.
Cereb Cortex ; 29(11): 4506-4518, 2019 12 17.
Article in English | MEDLINE | ID: mdl-30590449

ABSTRACT

Traumatic brain injury (TBI) results in a decrease in glutamate transporter-1 (GLT-1) expression, the major mechanism for glutamate removal from synapses. Coupled with an increase in glutamate release from dead and dying neurons, this causes an increase in extracellular glutamate. The ensuing glutamate excitotoxicity disproportionately damages vulnerable GABAergic parvalbumin-positive inhibitory interneurons, resulting in a progressively worsening cortical excitatory:inhibitory imbalance due to a loss of GABAergic inhibitory tone, as evidenced by chronic post-traumatic symptoms such as epilepsy, and supported by neuropathologic findings. This loss of intracortical inhibition can be measured and followed noninvasively using long-interval paired-pulse transcranial magnetic stimulation with mechanomyography (LI-ppTMS-MMG). Ceftriaxone, a ß-lactam antibiotic, is a potent stimulator of the expression of rodent GLT-1 and would presumably decrease excitotoxic damage to GABAergic interneurons. It may thus be a viable antiepileptogenic intervention. Using a rat fluid percussion injury TBI model, we utilized LI-ppTMS-MMG, quantitative PCR, and immunohistochemistry to test whether ceftriaxone treatment preserves intracortical inhibition and cortical parvalbumin-positive inhibitory interneuron function after TBI in rat motor cortex. We show that neocortical GLT-1 gene and protein expression are significantly reduced 1 week after TBI, and this transient loss is mitigated by ceftriaxone. Importantly, whereas intracortical inhibition declines progressively after TBI, 1 week of post-TBI ceftriaxone treatment attenuates the loss of inhibition compared to saline-treated controls. This finding is accompanied by significantly higher parvalbumin gene and protein expression in ceftriaxone-treated injured rats. Our results highlight prospects for ceftriaxone as an intervention after TBI to prevent cortical inhibitory interneuron dysfunction, partly by preserving GLT-1 expression.


Subject(s)
Anti-Bacterial Agents/administration & dosage , Brain Injuries, Traumatic/metabolism , Ceftriaxone/administration & dosage , Excitatory Amino Acid Transporter 2/metabolism , GABAergic Neurons/metabolism , Interneurons/metabolism , Animals , Disease Models, Animal , Gene Expression , Male , Motor Cortex/physiopathology , Parvalbumins/metabolism , Rats, Sprague-Dawley
18.
J Neurosci ; 37(45): 10783-10791, 2017 11 08.
Article in English | MEDLINE | ID: mdl-29118206

ABSTRACT

Adversity in early childhood exerts an enduring impact on mental and physical health, academic achievement, lifetime productivity, and the probability of interfacing with the criminal justice system. More science is needed to understand how the brain is affected by early life stress (ELS), which produces excessive activation of stress response systems broadly throughout the child's body (toxic stress). Our research examines the importance of sex, timing and type of stress exposure, and critical periods for intervention in various brain systems across species. Neglect (the absence of sensitive and responsive caregiving) or disrupted interaction with offspring induces robust, lasting consequences in mice, monkeys, and humans. Complementary assessment of internalizing disorders and brain imaging in children suggests that early adversity can interfere with white matter development in key brain regions, which may increase risk for emotional difficulties in the long term. Neural circuits that are most plastic during ELS exposure in monkeys sustain the greatest change in gene expression, offering a mechanism whereby stress timing might lead to markedly different long-term behaviors. Rodent models reveal that disrupted maternal-infant interactions yield metabolic and behavioral outcomes often differing by sex. Moreover, ELS may further accelerate or delay critical periods of development, which reflect GABA circuit maturation, BDNF, and circadian Clock genes. Such factors are associated with several mental disorders and may contribute to a premature closure of plastic windows for intervention following ELS. Together, complementary cross-species studies are elucidating principles of adaptation to adversity in early childhood with molecular, cellular, and whole organism resolution.


Subject(s)
Child Abuse/psychology , Developmental Disabilities/etiology , Social Environment , Adult , Child , Child, Preschool , Developmental Disabilities/psychology , Foster Home Care/psychology , Humans , Stress, Psychological/psychology
19.
Vis Neurosci ; 35: E014, 2018 01.
Article in English | MEDLINE | ID: mdl-29905116

ABSTRACT

The shift in ocular dominance (OD) of binocular neurons induced by monocular deprivation is the canonical model of synaptic plasticity confined to a postnatal critical period. Developmental constraints on this plasticity not only lend stability to the mature visual cortical circuitry but also impede the ability to recover from amblyopia beyond an early window. Advances with mouse models utilizing the power of molecular, genetic, and imaging tools are beginning to unravel the circuit, cellular, and molecular mechanisms controlling the onset and closure of the critical periods of plasticity in the primary visual cortex (V1). Emerging evidence suggests that mechanisms enabling plasticity in juveniles are not simply lost with age but rather that plasticity is actively constrained by the developmental up-regulation of molecular 'brakes'. Lifting these brakes enhances plasticity in the adult visual cortex, and can be harnessed to promote recovery from amblyopia. The reactivation of plasticity by experimental manipulations has revised the idea that robust OD plasticity is limited to early postnatal development. Here, we discuss recent insights into the neurobiology of the initiation and termination of critical periods and how our increasingly mechanistic understanding of these processes can be leveraged toward improved clinical treatment of adult amblyopia.


Subject(s)
Amblyopia/physiopathology , Critical Period, Psychological , Neuronal Plasticity/physiology , Adult , Child , Dominance, Ocular/physiology , Humans , Visual Cortex/physiology
20.
Cereb Cortex ; 27(12): 5509-5524, 2017 12 01.
Article in English | MEDLINE | ID: mdl-27909008

ABSTRACT

Many neuropsychiatric symptoms that follow traumatic brain injury (TBI), including mood disorders, sleep disturbance, chronic pain, and posttraumatic epilepsy (PTE) are attributable to compromised cortical inhibition. However, the temporal trajectory of cortical inhibition loss and its underlying mechanisms are not known. Using paired-pulse transcranial magnetic stimulation (ppTMS) and immunohistochemistry, we tracked functional and cellular changes of cortical inhibitory network elements after fluid-percussion injury (FPI) in rats. ppTMS revealed a progressive loss of cortical inhibition as early as 2 weeks after FPI. This profile paralleled the increasing levels of cortical oxidative stress, which was accompanied by a gradual loss of parvalbumin (PV) immunoreactivity in perilesional cortex. Preceding the PV loss, we identified a degradation of the perineuronal net (PNN)-a specialized extracellular structure enwrapping cortical PV-positive (PV+) inhibitory interneurons which binds the PV+ cell maintenance factor, Otx2. The trajectory of these impairments underlies the reduced inhibitory tone, which can contribute to posttraumatic neurological conditions, such as PTE. Taken together, our results highlight the use of ppTMS as a biomarker to track the course of cortical inhibitory dysfunction post-TBI. Moreover, the neuroprotective role of PNNs on PV+ cell function suggests antioxidant treatment or Otx2 enhancement as a promising prophylaxis for post-TBI symptoms.


Subject(s)
Brain Injuries, Traumatic/physiopathology , Cerebral Cortex/physiopathology , Interneurons/metabolism , Neural Inhibition/physiology , Parvalbumins/metabolism , Animals , Brain Injuries, Traumatic/pathology , Cerebral Cortex/pathology , Disease Models, Animal , Disease Progression , Extracellular Matrix/metabolism , Extracellular Matrix/pathology , Functional Laterality , Interneurons/pathology , Male , Otx Transcription Factors/metabolism , Oxidative Stress/physiology , Rats, Long-Evans , Transcranial Magnetic Stimulation
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