ABSTRACT
Fecal samples (n = 531) submitted to a regional clinical laboratory during a 6-month period were tested for the presence of Shiga toxin using both a Vero cell cytotoxicity assay and the Shiga Toxin Quik Chek test (STQC), a rapid membrane immunoassay. Testing the samples directly (without culture), 9 positives were identified by the Vero cell assay, all of which were also detected by the STQC. The correlation between the two assays was 100%. Not all of the identified positive samples were detected when fecal broth cultures were tested. By testing broth cultures of characterized isolates representing all described Shiga toxin subtypes, the STQC detected all subtypes. Levels of induction of toxin production by ciprofloxacin differed among the strains tested, with more toxin induction seen in strains harboring Stx2 phages than in those harboring Stx1 phages.
Subject(s)
Ciprofloxacin/pharmacology , Escherichia coli Infections/diagnosis , Escherichia coli O157/isolation & purification , Feces/microbiology , Shiga Toxin 1/biosynthesis , Shiga Toxin 2/biosynthesis , Animals , Cell Line , Chlorocebus aethiops , Escherichia coli Infections/microbiology , Humans , Immunoassay/methods , Vero CellsABSTRACT
A novel fecal antigen detection assay for fresh and frozen human samples that detects but does not differentiate Giardia spp, Cryptosporidium spp, and Entamoeba histolytica, the Tri-Combo parasite screen, was compared to three established enzyme-linked immunosorbent assays (ELISAs) at three international sites. It exhibited 97.9% sensitivity and 97.0% specificity, with positive and negative predictive values of 93.4% and 99.1%, respectively. The Tri-Combo test proved a reliable means to limit the use of individual parasite ELISAs to positive samples.
Subject(s)
Antigens, Protozoan/analysis , Clinical Laboratory Techniques/methods , Cryptosporidium/isolation & purification , Entamoeba histolytica/isolation & purification , Feces/parasitology , Giardia/isolation & purification , Parasitology/methods , Adolescent , Adult , Aged , Aged, 80 and over , Child , Child, Preschool , Enzyme-Linked Immunosorbent Assay/methods , Female , Humans , Infant , Male , Middle Aged , Sensitivity and Specificity , Young AdultABSTRACT
BACKGROUND: An evaluation of currently available in-clinic diagnostic tests for Giardia duodenalis infection of dogs and cats has not been performed. In addition, there is discordance among published diagnostic comparisons. The absence of a true gold standard for detecting Giardia duodenalis also complicates diagnostic evaluations. OBJECTIVES: To evaluate diagnostic tests commercially available in the United States for detecting Giardia duodenalis in dogs and cats, in comparison to a widely used reference test, the direct immunofluorescent assay (IFA), and also to compare the results of 2 methods of analysis: comparison of diagnostic tests to a reference test (IFA) and Bayesian analysis. ANIMALS: Fecal samples from a convenience sample of 388 cats and dogs located in Colorado, Oklahoma, and Virginia. METHODS: Fecal samples were tested for Giardia duodenalis by zinc sulfate centrifugal fecal flotation and 4 different commercial diagnostic immunoassays. Results were analyzed via Bayesian analysis and by comparison to the IFA as the reference test. RESULTS: Sensitivity and specificity by comparison to IFA was ≥82% and ≥90%, respectively, for all diagnostic tests in dogs and cats. When analyzed via Bayesian analysis, sensitivity and specificity were ≥83% and ≥95%, respectively. When ZnSO4 centrifugal fecal flotation results were combined with immunoassay results, there was no longer a significant difference between the sensitivities of the commercial in-clinic immunoassays. CONCLUSION AND CLINICAL RELEVANCE: The Bayesian analysis validates using IFA as the reference test. Differences in commercial in-clinic immunoassay sensitivities can be mitigated when the results are combined with ZnSO4 centrifugal fecal flotation results.
Subject(s)
Cat Diseases/parasitology , Dog Diseases/parasitology , Feces/parasitology , Fluorescent Antibody Technique, Direct/veterinary , Giardiasis/veterinary , Animals , Cat Diseases/diagnosis , Cats , Centrifugation/methods , Centrifugation/veterinary , Dog Diseases/diagnosis , Dogs , Fluorescent Antibody Technique, Direct/methods , Giardiasis/diagnosis , Sensitivity and Specificity , United StatesABSTRACT
Several commercial Giardia immunoassays were evaluated in baboons for sensitivity and specificity as well as ease of use in a large specific pathogen-free (SPF) colony. An additional objective was to identify the assemblage(s) of Giardia duodenalis present in this baboon colony. A direct immunofluorescent antibody test (IFAT) was used as the reference test. Tests evaluated were a patient-side rapid test for dogs and cats, a human rapid test, and a well-plate ELISA designed for use with humans. Test sensitivities and specificities were compared using the McNemar paired t-test and were further evaluated for agreement using an unweighted Cohen kappa statistic. When compared to the IFAT reference, both human tests were more sensitive than the veterinary test. Based on PCR and sequencing of the G. duodenalis small-subunit ribosomal RNA and glutamate dehydrogenase loci, assemblage AI was present in this baboon colony. We found that 10 of the 110 (9%) baboons in this SPF colony were infected with a zoonotic strain of G. duodenalis.
Subject(s)
Giardia lamblia/isolation & purification , Giardiasis/veterinary , Immunoassay/veterinary , Monkey Diseases/diagnosis , Papio anubis , Animals , Animals, Laboratory , Enzyme-Linked Immunosorbent Assay/veterinary , Fluorescent Antibody Technique, Indirect/veterinary , Giardiasis/diagnosis , Giardiasis/epidemiology , Giardiasis/parasitology , Immunoassay/methods , Monkey Diseases/epidemiology , Monkey Diseases/parasitology , Sensitivity and Specificity , Specific Pathogen-Free OrganismsABSTRACT
Amebiasis is a major cause of morbidity and mortality in the developing world. A reliable point-of-care test would help to improve diagnosis and early treatment. We evaluated a novel rapid fecal antigen detection test for E. histolytica (E. HISTOLYTICA QUIK CHEK; TechLab, Inc., Blacksburg, VA), in a cohort of children in Bangladesh where amebiasis is endemic. This point-of-care test had a sensitivity of 100% and a specificity of 100% when compared with enzyme-linked immunosorbent assay antigen detection.