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1.
PLoS Biol ; 20(3): e3001589, 2022 03.
Article in English | MEDLINE | ID: mdl-35324891

ABSTRACT

Clostridioides difficile secretes Toxin B (TcdB) as one of its major virulence factors, which binds to intestinal epithelial and subepithelial receptors, including frizzled proteins and chondroitin sulfate proteoglycan 4 (CSPG4). Here, we present cryo-EM structures of full-length TcdB in complex with the CSPG4 domain 1 fragment (D1401-560) at cytosolic pH and the cysteine-rich domain of frizzled-2 (CRD2) at both cytosolic and acidic pHs. CSPG4 specifically binds to the autoprocessing and delivery domains of TcdB via networks of salt bridges, hydrophobic and aromatic/proline interactions, which are disrupted upon acidification eventually leading to CSPG4 drastically dissociating from TcdB. In contrast, FZD2 moderately dissociates from TcdB under acidic pH, most likely due to its partial unfolding. These results reveal structural dynamics of TcdB during its preentry step upon endosomal acidification, which provide a basis for developing therapeutics against C. difficile infections.


Subject(s)
Bacterial Toxins , Clostridioides difficile , Bacterial Proteins/metabolism , Bacterial Toxins/metabolism , Protein Domains , Virulence Factors/metabolism
3.
PLoS Biol ; 17(6): e3000311, 2019 06.
Article in English | MEDLINE | ID: mdl-31233493

ABSTRACT

Clostridium difficile infection (CDI) is a major nosocomial disease associated with significant morbidity and mortality. The pathology of CDI stems primarily from the 2 C. difficile-secreted exotoxins-toxin A (TcdA) and toxin B (TcdB)-that disrupt the tight junctions between epithelial cells leading to the loss of colonic epithelial barrier function. Here, we report the engineering of a series of monomeric and dimeric designed ankyrin repeat proteins (DARPins) for the neutralization of TcdB. The best dimeric DARPin, DLD-4, inhibited TcdB with a half maximal effective concentration (EC50) of 4 pM in vitro, representing an approximately 330-fold higher potency than the Food and Drug Administration (FDA)-approved anti-TcdB monoclonal antibody bezlotoxumab in the same assay. DLD-4 also protected mice from a toxin challenge in vivo. Cryo-electron microscopy (cryo-EM) studies revealed that the 2 constituent DARPins of DLD-4-1.4E and U3-bind the central and C-terminal regions of the delivery domain of TcdB. Competitive enzyme-linked immunosorbent assay (ELISA) studies showed that the DARPins 1.4E and U3 interfere with the interaction between TcdB and its receptors chondroitin sulfate proteoglycan 4 (CSPG4) and frizzled class receptor 2 (FZD2), respectively. Our cryo-EM studies revealed a new conformation of TcdB (both apo- and DARPin-bound at pH 7.4) in which the combined repetitive oligopeptides (CROPS) domain points away from the delivery domain. This conformation of the CROPS domain is in stark contrast to that seen in the negative-stain electron microscopy (EM) structure of TcdA and TcdB at the same pH, in which the CROPS domain bends toward and "kisses" the delivery domain. The ultrapotent anti-TcdB molecules from this study serve as candidate starting points for CDI drug development and provide new biological tools for studying the pathogenicity of C. difficile. The structural insights regarding both the "native" conformation of TcdB and the putative sites of TcdB interaction with the FZD2 receptor, in particular, should help accelerate the development of next-generation anti-C. difficile toxin therapeutics.


Subject(s)
Bacterial Proteins/antagonists & inhibitors , Bacterial Proteins/physiology , Bacterial Toxins/antagonists & inhibitors , Clostridium Infections/metabolism , Animals , Ankyrin Repeat/genetics , Antibodies, Monoclonal , Bacterial Proteins/genetics , Bacterial Proteins/metabolism , Bacterial Toxins/genetics , Bacterial Toxins/metabolism , Broadly Neutralizing Antibodies , Caco-2 Cells , Clostridioides difficile/metabolism , Clostridioides difficile/pathogenicity , Cryoelectron Microscopy , Enterotoxins/metabolism , Humans , Mice , Protein Engineering/methods
4.
Traffic ; 19(6): 421-435, 2018 06.
Article in English | MEDLINE | ID: mdl-29582528

ABSTRACT

Various densely charged polycationic species, whether of biological or synthetic origin, can penetrate human cells, albeit with variable efficiencies. The molecular underpinnings involved in such transport remain unclear. Herein, we assemble 1, 2 or 3 copies of the HIV peptide TAT on a synthetic scaffold to generate branched cell-permeable prototypes with increasing charge density. We establish that increasing TAT copies dramatically increases the cell penetration efficiency of the peptides while simultaneously enabling the efficient cytosolic delivery of macromolecular cargos. Cellular entry involves the leaky fusion of late endosomal membranes enriched with the anionic lipid BMP. Derivatives with multiple TAT branches induce the leakage of BMP-containing lipid bilayers, liposomal flocculation, fusion and an increase in lamellarity. In contrast, while the monomeric counterpart 1TAT binds to the same extent and causes liposomal flocculation, 1TAT does not cause leakage, induce fusion or a significant increase in lamellarity. Overall, these results indicate that an increase in charge density of these branched structures leads to the emergence of lipid specific membrane-disrupting and cell-penetrating activities.


Subject(s)
Endosomes/metabolism , Lipids/chemistry , Peptides/metabolism , Cell Line, Tumor , Cytosol/metabolism , HeLa Cells , Humans , Intracellular Membranes/metabolism , Lipid Bilayers/metabolism , Liposomes/metabolism
5.
Proc Natl Acad Sci U S A ; 114(15): 4011-4016, 2017 04 11.
Article in English | MEDLINE | ID: mdl-28348234

ABSTRACT

MicroRNA (miRNA) is processed from primary transcripts with hairpin structures (pri-miRNAs) by microprocessors in the nucleus. How cytoplasmic-borne microprocessor components are transported into the nucleus to fulfill their functions remains poorly understood. Here, we report KETCH1 (karyopherin enabling the transport of the cytoplasmic HYL1) as a partner of hyponastic leaves 1 (HYL1) protein, a core component of microprocessor in Arabidopsis and functional counterpart of DGCR8/Pasha in animals. Null mutation of ketch1 is embryonic-lethal, whereas knockdown mutation of ketch1 caused morphological defects, reminiscent of mutants in the miRNA pathway. ketch1 knockdown mutation also substantially reduced miRNA accumulation, but did not alter nuclear-cytoplasmic shuttling of miRNAs. Rather, the mutation significantly reduced nuclear portion of HYL1 protein and correspondingly compromised the pri-miRNA processing in the nucleus. We propose that KETCH1 transports HYL1 from the cytoplasm to the nucleus to constitute functional microprocessor in Arabidopsis This study provides insight into the largely unknown nuclear-cytoplasmic trafficking process of miRNA biogenesis components through eukaryotes.


Subject(s)
Arabidopsis Proteins/metabolism , Arabidopsis/genetics , Cell Nucleus/metabolism , MicroRNAs/metabolism , RNA-Binding Proteins/metabolism , Arabidopsis/metabolism , Arabidopsis Proteins/genetics , Cell Nucleus/genetics , Gene Expression Regulation, Plant , Karyopherins , MicroRNAs/genetics , Mutation , Plants, Genetically Modified , Protein Transport , RNA Processing, Post-Transcriptional , RNA-Binding Proteins/genetics , Nicotiana/genetics , Nicotiana/metabolism
6.
Bioengineering (Basel) ; 9(10)2022 Sep 27.
Article in English | MEDLINE | ID: mdl-36290479

ABSTRACT

Shiga toxin-producing E. coli (STEC) is a common cause of bloody diarrhea. The pathology of STEC infection derives from two exotoxins-Shiga toxin 1 (Stx1) and Shiga toxin 2 (Stx2)-that are secreted by STEC in the gut, from where they are systemically absorbed, causing severe kidney damage leading to hemolytic uremic syndrome (HUS). Currently, there is no effective treatment for HUS, and only supportive care is recommended. We report the engineering of a panel of designed ankyrin repeat proteins (DARPin) with potent neutralization activity against Stx2a, the major subtype associated with HUS. The best dimeric DARPin, SD5, created via a combination of directed evolution and rational design, neutralizes Stx2a with a half maximal effective concentration (EC50) of 0.61 nM in vitro. The two monomeric DARPin constituents of SD5 exhibit complementary functions-SHT targets the enzymatic A subunit of Stx2a and inhibits the toxin's catalytic activity, while DARPin #3 binds the B subunit, based on the cryo-EM study, and induces a novel conformational change in the B subunit that distorts its five-fold symmetry and presumably interferes with toxin attachment to target cells. SD5 was fused to an albumin-binding DARPin, and the resulting trimeric DARPin DA1-SD5 efficiently protects mice in a toxin challenge model, pointing to a high potential of this DARPin as a therapeutic for STEC infection. Finally, the unprecedented toxin conformational change induced by DARPin #3 represents a novel mode of action for neutralizing Stx2 toxicity and reveals new targets for future drug development.

7.
Cell Chem Biol ; 27(10): 1296-1307.e5, 2020 10 15.
Article in English | MEDLINE | ID: mdl-32783962

ABSTRACT

Many cellular delivery reagents enter the cytosolic space of cells by escaping the lumen of endocytic organelles and, more specifically, late endosomes. The mechanisms involved in endosomal membrane permeation remain largely unresolved, which impedes the improvement of delivery agents. Here, we investigate how 3TAT, a branched analog of the cell-penetrating peptide (CPP) TAT, achieves the permeabilization of bilayers containing bis(monoacylglycero)phosphate (BMP), a lipid found in late endosomes. We establish that the peptide does not induce the leakage of individual lipid bilayers. Instead, leakage requires contact between membranes. Peptide-driven bilayer contacts lead to fusion, lipid mixing, and, critically, peptide encapsulation within proximal bilayers. Notably, this encapsulation is a distinctive property of BMP that explains the specificity of CPP's membrane leakage activity. These results therefore support a model of cell penetration that requires both BMP and the vicinity between bilayers, two features unique to BMP-rich and multivesicular late endosomes.


Subject(s)
Cell-Penetrating Peptides/metabolism , Endosomes/metabolism , Lysophospholipids/metabolism , Monoglycerides/metabolism , Animals , Cell Line , Cell-Penetrating Peptides/chemistry , Cricetulus , Endosomes/chemistry , Humans , Lipid Bilayers/chemistry , Lipid Bilayers/metabolism , Lysophospholipids/chemistry , Monoglycerides/chemistry
8.
Nat Commun ; 10(1): 3130, 2019 07 16.
Article in English | MEDLINE | ID: mdl-31311931

ABSTRACT

Single-stranded RNA bacteriophages (ssRNA phages) infect Gram-negative bacteria via a single maturation protein (Mat), which attaches to a retractile pilus of the host. Here we present structures of the ssRNA phage MS2 in complex with the Escherichia coli F-pilus, showing a network of hydrophobic and electrostatic interactions at the Mat-pilus interface. Moreover, binding of the pilus induces slight orientational variations of the Mat relative to the rest of the phage capsid, priming the Mat-connected genomic RNA (gRNA) for its release from the virions. The exposed tip of the attached Mat points opposite to the direction of the pilus retraction, which may facilitate the translocation of the gRNA from the capsid into the host cytosol. In addition, our structures determine the orientation of the assembled F-pilin subunits relative to the cell envelope, providing insights into the F-like type IV secretion systems.


Subject(s)
Escherichia coli/virology , Levivirus/ultrastructure , Cell Wall/metabolism , Cell Wall/ultrastructure , Cell Wall/virology , Cryoelectron Microscopy , Escherichia coli/ultrastructure , Escherichia coli Proteins/metabolism , Escherichia coli Proteins/ultrastructure , Fimbriae Proteins/metabolism , Fimbriae Proteins/ultrastructure , Fimbriae, Bacterial/metabolism , Fimbriae, Bacterial/ultrastructure , Fimbriae, Bacterial/virology , Levivirus/genetics , RNA, Guide, Kinetoplastida/metabolism , RNA, Viral/metabolism , Viral Proteins/ultrastructure
9.
Nat Commun ; 8: 15934, 2017 06 30.
Article in English | MEDLINE | ID: mdl-28665408

ABSTRACT

Many essential proteins cannot fold without help from chaperonins, like the GroELS system of Escherichia coli. How chaperonins accelerate protein folding remains controversial. Here we test key predictions of both passive and active models of GroELS-stimulated folding, using the endogenous E. coli metalloprotease PepQ. While GroELS increases the folding rate of PepQ by over 15-fold, we demonstrate that slow spontaneous folding of PepQ is not caused by aggregation. Fluorescence measurements suggest that, when folding inside the GroEL-GroES cavity, PepQ populates conformations not observed during spontaneous folding in free solution. Using cryo-electron microscopy, we show that the GroEL C-termini make physical contact with the PepQ folding intermediate and help retain it deep within the GroEL cavity, resulting in reduced compactness of the PepQ monomer. Our findings strongly support an active model of chaperonin-mediated protein folding, where partial unfolding of misfolded intermediates plays a key role.


Subject(s)
Dipeptidases/chemistry , Escherichia coli Proteins/metabolism , Escherichia coli/enzymology , Heat-Shock Proteins/metabolism , Dipeptidases/genetics , Dipeptidases/metabolism , Escherichia coli/chemistry , Escherichia coli/genetics , Escherichia coli/metabolism , Escherichia coli Proteins/genetics , Heat-Shock Proteins/genetics , Protein Conformation , Protein Folding
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