ABSTRACT
The influence of low-energy millimeter electromagnetic waves on aqueous saline solution of DNA from the liver of healthy rats and rats with sarcoma 45 has been investigated. The characteristic parameters of irradiated and unirradiated DNA, melting temperature, and the range of melting were obtained from melting curves. The duration of exposure did not practically affect the range of melting, while the thermostability of DNA increased; as irradiation duration increased to 90 min, the melting temperature of tumor increased by approximately 1.5 degrees C. It was assumped that the increase in the thermostability of DNA is due to a more effective stabilization of the DNA double helix caused by the dehydration of Na(+)- ions present in the solution.
Subject(s)
DNA/radiation effects , Nucleic Acid Conformation/radiation effects , Transition Temperature/radiation effects , Animals , DNA/chemistry , DNA/isolation & purification , Electromagnetic Phenomena , Liver/chemistry , Liver/radiation effects , Liver Neoplasms , Rats , Sarcoma , Sodium/chemistry , Solutions/chemistryABSTRACT
In conditions of acute experiment on white rats anaesthetized by Nembutal (40 mg/kg, intraperitoneally), the registration and analysis of background impulse activity of the supraoptic nucleus neurons of rats' hypothalamus in norm and after electromagnetic irradiation of millimeter wavelength on organism, were carried out. Distributions of neurons by the degree of rhythm regularity, the character of types of dynamics of the following impulse flows, the modality of histograms of interspike intervals, the average discharge frequency, the coefficient of interspike intervals variation, were found out. Changes of the background impulse activity which were related mainly to the changes of the inner structure of registered impulse flows, were revealed. Significant shifts were generally observed in the character of dynamics of neuronal current flows and degree of regularity of the impulse activity. Statistically significant changes of the average frequency of discharges of different frequency range neurons' populations were revealed.