Antimicrobial Resistance Profiles and Molecular Characteristics of Salmonella enterica Serovar Agona Isolated from Food-Producing Animals During 2010-2020 in South Korea.

Multidrug-resistant (MDR) Salmonella enterica serovar Agona infections affect public health globally. This investigation aimed to ascertain the antimicrobial resistance profiles and molecular characteristics of Salmonella Agona isolates obtained from food-producing animals. A total of 209 Salmonella Agona isolates were recovered from mostly chickens (139 isolates), pigs (56 isolates), cattle (11 isolates), and ducks (3 isolates) between 2010 and 2020 in South Korea. In addition, these Salmonella Agona isolates were obtained from 25 slaughterhouses nationwide. Furthermore, this serotype suddenly increased in chickens in 2020. Salmonella Agona from chickens showed high resistance (69-83%) to ampicillin, streptomycin, tetracycline, trimethoprim/sulfamethoxazole, and chloramphenicol. Moreover, chicken/duck isolates (83.1%) showed significantly higher levels of MDR than cattle/pig isolates (1.5%). For molecular analysis by pulsed-field gel electrophoresis, infrared spectroscopy biotyping, and multilocus sequence typing in combination, a total of 23 types were observed. Especially two major types, P1-III-2-13 and P1-IV-2-13, comprised 59.3% of the total isolates spreading in most farms. Moreover, Salmonella Agona sequence type (ST)13 was predominant (96.7%) among three different STs (ST13, ST11, and ST292) widely detected in chickens (94.3%) in most farms located nationwide. Taken together, MDR Salmonella Agona in chickens might pose a potential risk to public health through direct contact or the food chain.

Sub-10 fs pulse generation by post-compression for peak-power enhancement of a 100-TW Ti:Sapphire laser.

We demonstrated sub-10 fs pulse generation by the post-compression of a 100 TW Ti:Sapphire laser to enhance the peak-power. In the post-compression, the laser spectrum was widely broadened by self-phase modulation in thin fused silica plate(s), and the induced spectral phase was compensated with a set of chirped mirrors. A spatial filter stage, consisting of two cylindrical lenses and a spherical lens, was employed to reduce the intensity modulation existing in the laser beam, which effectively suppressed intensity spikes induced by self-focusing. The laser beam was post-compressed from 23 fs to 9.7 fs after propagating through a 1.5 mm fused silica plate, resulting in the peak-power enhancement by a factor of 2.1.

Wavefront-corrected post-compression of a 100-TW Ti:sapphire laser.

We analyzed and corrected the wavefront distortion induced during the post-compression of a 100-TW Ti:Sapphire laser and achieved the intensity enhancement. In the post-compression, the spectral broadening of the laser was obtained by propagating through three 0.5 mm-thick fused silica plates and the laser pulse duration was post-compressed from 24 fs to 11 fs using a set of chirped mirrors. We measured the wavefront aberrations due to the intensity-dependent nonlinear process during the post-compression of femtosecond high-power laser pulses. By compensating for the wavefront aberrations with an adaptive optics system, the Strehl ratio of the post-compressed beam was improved from 0.37 to 0.52 and the focused intensity of the post-compressed beam could be enhanced by a factor of 1.5, while the enhancement without the wavefront correction was only a factor of 1.1 in spite of the peak-power enhancement by a factor of 1.8.

Single-shot spatiotemporal characterization of a multi-PW laser using a multispectral wavefront sensing method.

The single-shot spatiotemporal characterization of an ultrahigh intensity laser pulse was performed using a multispectral wavefront sensor. For the measurement of the spatio-spectral electric field, a femtosecond laser pulse was spectrally modulated and separated by a Fabry-Perot etalon coupled with a grating pair, and its spatio-spectral electric field was measured with a wavefront sensor. The spatiotemporal electric field was reconstructed from the measured spatio-spectral electric field of a multi-PW laser pulse. We found that the spatiotemporal distortion could reduce the focused laser intensity by 15%, compared to the case of a diffraction-limited and transform-limited laser pulse.

Analysis of angular dispersion induced by wavefront rotation in nanosecond optical parametric chirped pulse amplification.

Angular dispersion observed in a nanosecond optical parametric chirped-pulse amplification (ns-OPCPA) amplifier adopted in the frontend of a multi-PW laser was analyzed. The theory on the angular dispersion, extended by including the wavefront rotation and the pulse front tilt of a strongly chirped laser pulse, revealed that the wavefront rotation is a major contributor to the angular dispersion, as compared to the pulse front tilt, in a ns-OPCPA amplifier. It was also shown that the wavefront rotation could be introduced by the phase mismatch and the noncollinear propagation angle in the noncollinear ns-OPCPA amplifier. The theoretical prediction was experimentally verified by measuring the angular dispersion of the ns-OPCPA frontend installed in the 20-fs, 4-PW Ti:Sapphire laser. We emphasize the importance of the proper characterization and control of the angular dispersion in the ns-OPCPA amplifier since the focus intensity of an ultrahigh power laser could be significantly reduced due to the spatiotemporal effect even for small induced angular dispersion.

IoT-Based Resource Control for In-Vehicle Infotainment Services: Design and Experimentation.

A variety of in-vehicle infotainment (IVI) devices and services have been developed by many vehicle vendors and software companies, which include navigation systems, cameras, speakers, headrest displays, and heating seat. However, there has not been enough research on how to effectively control and manage numerous IVI resources (devices and contents), so as to provide users with more enhanced services. This paper proposes a framework of resource control for IVI services so as to efficiently manage the IVI resources within an automobile. Differently from conventional IVI systems, in the proposed scheme, the IVI-Master is newly introduced for overall control of IVI resources, and IVI users are divided into owner and users. In addition, the IVI resources are classified as personal resources and shared resources, which are managed by the IVI-Master using the Lightweight Machine-to-Machine (LWM2M) standard. The proposed IoT-based IVI resource control scheme was implemented and tested. The experimental results showed that the proposed scheme can be used to effectively manage IVI resources for users. Additionally, the proposed resource control scheme shows lower bandwidth usage than the existing scheme.

Health Care Experiences of Korean Women Divers (Jeju Haenyeos).

Jeju haenyeos who enter the sea without equipment to collect seafood while holding their breath have a unique culture; however, studies of their health are limited. This study, using an ethnographic approach, explores how Jeju haenyeos have managed and maintained their health. Snowball sampling yielded 15 participants between July and October 2016. We collected data through participant observation and in-depth interviews and conducted Spradley's research sequence. The main theme of the health-management approach of these women was "a life of listening to the body and mind, controlling greed, and adjusting work for safe diving." However, Jeju haenyeos used preventive drugs and excessive doses of other drugs and lacked an understanding of the ways diverse drugs potentially interact. These findings suggest that community nurses should be cautious and should attempt to educate these patients.

Tracking motor impairments in the progression of Huntington's disease.

The Unified Huntington's Disease Rating Scale is used to characterize motor impairments and establish motor diagnosis. Little is known about the timing of diagnostic confidence level categories and the trajectory of motor impairments during the prodromal phase. Goals of this study were to estimate the timing of categories, model the prodromal trajectory of motor impairments, estimate the rate of motor impairment change by category, and provide required sample size estimates for a test of efficacy in clinical trials. In total, 1010 gene-expanded participants from the Neurobiological Predictors of Huntington's Disease (PREDICT-HD) trial were analyzed. Accelerated failure time models were used to predict the timing of categories. Linear mixed effects regression was used to model the longitudinal motor trajectories. Age and length of gene expansion were incorporated into all models. The timing of categories varied significantly by gene expansion, with faster progression associated with greater expansion. For the median expansion, the third diagnostic confidence level category was estimated to have a first occurrence 1.5 years before diagnosis, and the second and first categories were estimated to occur 6.75 years and 19.75 years before diagnosis, respectively. Motor impairments displayed a nonlinear prodromal course. The motor impairment rate of change increased as the diagnostic confidence level increased, with added acceleration for higher progression scores. Motor items can detect changes in motor impairments before diagnosis. Given a sufficiently high progression score, there is evidence that the diagnostic confidence level can be used for prodromal staging. Implications for Huntington's disease research and the planning of clinical trials of efficacy are discussed.

The association between migraine and Parkinson's disease: a nationwide cohort study.

OBJECTIVES: Clinical studies have suggested an association between migraine and the occurrence of Parkinson's disease (PD). However, it is unknown whether migraine affects PD risk. We aimed to investigate the incidence of PD in patients with migraine and to determine the risk factors affecting the association between migraine and PD incidence. METHODS: Using the Korean National Health Insurance System database (2002-2019), we enrolled all Koreans aged ≥40 years who participated in the national health screening program in 2009. International Classification of Diseases (10th revision) diagnostic codes and Rare Incurable Diseases System diagnostic codes were used to define patients with migraine (within 12 months of enrollment) and newly diagnosed PD. RESULTS: We included 214,193 patients with migraine and 5,879,711 individuals without migraine. During 9.1 years of follow-up (55,435,626 person-years), 1,973 (0.92%) and 30,664 (0.52%) individuals with and without migraine, respectively, were newly diagnosed with PD. Following covariate adjustment, patients with migraine showed a 1.35-fold higher PD risk than individuals without migraine. The incidence of PD was not significantly different between patients with migraine with aura and those without aura. In males with migraine, underlying dyslipidemia increased the risk of PD (p=0.012). In contrast, among females with migraine, younger age (<65 years) increased the risk of PD (p=0.038). CONCLUSIONS: Patients with migraine were more likely to develop PD than individuals without migraine. Preventive management of underlying comorbidities and chronic migraine may affect the incidence of PD in these patients. Future prospective randomized clinical trials are warranted to clarify this association.

3D Printing of CNT- and YSZ-Added Dental Resin-Based Composites by Digital Light Processing and Their Mechanical Properties.

This study demonstrates the successful 3D printing of dental resin-based composites (DRCs) containing ceramic particles using the digital light processing (DLP) technique. The mechanical properties and oral rinsing stability of the printed composites were evaluated. DRCs have been extensively studied for restorative and prosthetic dentistry due to their clinical performance and aesthetic quality. They are often subjected to periodic environmental stress, and thus can easily undergo undesirable premature failure. Here, we investigated the effects of two different high-strength and biocompatible ceramic additives, carbon nanotube (CNT) and yttria-stabilized zirconia (YSZ), on the mechanical properties and oral rinsing stabilities of DRCs. Dental resin matrices containing different wt.% of CNT or YSZ were printed using the DLP technique after analyzing the rheological behavior of slurries. Mechanical properties such as Rockwell hardness and flexural strength, as well as the oral rinsing stability of the 3D-printed composites, were systematically investigated. The results indicated that a DRC with 0.5 wt.% YSZ exhibits the highest hardness of 19.8 ± 0.6 HRB and a flexural strength flexural strength of 50.6 ± 6 MPa, as well as reasonable oral rinsing steadiness. This study provides a fundamental perspective for designing advanced dental materials containing biocompatible ceramic particles.

Arthroscopic Repair of Large Subscapularis Tear Over the First Facet.

BACKGROUND: Long-term studies on arthroscopic repair of large subscapularis (SSC) tears over the first facet with or without supraspinatus (SSP) tear are limited. PURPOSE: To assess the structural and clinical outcomes of arthroscopic repair of large SSC tears using magnetic resonance imaging (MRI) and identify the factors related to SSC retear and poor outcomes. STUDY DESIGN: Case series; Level of evidence, 4. METHODS: This study involved 109 patients (84.0 ± 36.2 months of follow-up) who underwent arthroscopic repair of large SSC tears (Yoo and Rhee classification type III [n = 81] or IV [n = 28]) between 2011 and 2019. All patients underwent MRI at 6.1 ± 0.4 months after surgery, and 79 of 109 patients (72.5%) were followed up over 7 years. Clinical outcomes (active range of motion, functional scores, and belly press strength) and final poor clinical outcomes (reoperation, osteoarthritic change, and poor clinical outcome) were recorded. SSP tear size, rotator cuff atrophy and fatty infiltration (Goutallier classification), SSC tendon integrity (intact, intermediate, definite tear), and SSP tendon integrity (Sugaya classification) were evaluated by MRI. RESULTS: The overall SSC retear rate was 8.3% (9/109) (type III, 2/81 [2.5%]; type IV, 7/28 [25.0%]; P < .001). SSC Goutallier grade 4 showed a higher retear rate than grade 3 (7/33 [21.2%] vs 2/33 [6.1%]; P = .149). A large SSP tear (in millimeters) (odds ratio [OR], 1.5; P = .003), SSC Goutallier grade 4 (OR, 10.8; P = .047), and SSP Goutallier grade 3 or 4 (OR, 0.02; P = .013) were independent factors for SSC retear. Clinical outcomes, except for external rotation, were poorer in patients with SSC retear than in those without retear. Final poor clinical outcomes were observed in 27 of 79 patients (34.2%); female sex (OR, 7.7; P = .007), SSC retear (OR, 8.2; P = .025), and SSP retear (OR, 4.7; P = .031) were independent factors. CONCLUSION: Arthroscopic repair of large SSC tears has shown promising structural outcomes for type III tears but not type IV tears. SSC retear was affected by SSC atrophy, as well as SSP tear size and atrophy. Approximately one-third of the final poor clinical outcomes could be predicted, and SSC retear, SSP retear, and female sex were associated with long-term poor clinical outcomes, underscoring the importance of carefully selecting patients for arthroscopic repair of large SSC tears.

Antimicrobial Resistance in Escherichia coli Isolated from Healthy Dogs and Cats in South Korea, 2020-2022.

The occurrence of antimicrobial-resistant bacteria in companion animals poses public health hazards globally. This study aimed to evaluate the antimicrobial resistance profiles and patterns of commensal E. coli strains obtained from fecal samples of healthy dogs and cats in South Korea between 2020 and 2022. In total, 843 E. coli isolates (dogs, n = 637, and cats, n = 206) were assessed for susceptibility to 20 antimicrobials. The resistance rates of the most tested antimicrobials were significantly higher in dog than in cat isolates. Cefalexin (68.9%) demonstrated the highest resistance rates, followed by ampicillin (38.3%), tetracycline (23.1%), and cefazolin (18.7%). However, no or very low resistance (0-0.6%) to amikacin, imipenem, piperacillin, and colistin was found in both dog and cat isolates. Overall, 42.3% of the isolates exhibited multidrug resistance (MDR). MDR in isolates from dogs (34.9%) was significantly higher than in those from cats (20.9%). The main components of the resistance patterns were cefalexin and ampicillin in both dog and cat isolates. Additionally, MDR patterns in isolates from dogs (29.2%) and cats (16%) were shown to encompass five or more antimicrobials. Multidrug-resistant commensal E. coli could potentially be spread to humans or other animals through clonal or zoonotic transmission. Therefore, the incidence of antimicrobial resistance in companion animals highlights the urgent need to restrict antimicrobial resistance and ensure the prudent use of antimicrobials in Korea.

Relationship between Preventive Health Behavior, Optimistic Bias, Hypochondria, and Mass Psychology in Relation to the Coronavirus Pandemic among Young Adults in Korea.

The great challenge to global public health caused by the coronavirus pandemic has lasted for two years in Korea. However, Korean young adults seem less compliant with preventive health behaviors than older adults. This study aims to explore the relationship between risk perception variables of optimistic bias, hypochondriasis, and mass psychology, and preventive health behavior in relation to the coronavirus pandemic through a cross-sectional online survey. The participants are 91 Korean young adults aged 19-30. The results show that mass psychology has a positive relationship with preventive health behavior, whereas optimistic bias and hypochondriasis do not. In detail, people with high or middle levels of mass psychology displayed higher preventive health behavior compared with those who had low levels of mass psychology, and the highest compliance was for wearing a mask, followed by COVID-19 vaccination, whereas the lowest compliance was for influenza vaccination. These findings could be explained by the Korean culture of strong collectivism and the characteristics of COVID-19, which evoked extreme fear globally. The results of this study can be useful for policy establishment in the ongoing prevention of COVID-19 and suggest that mass psychology should be used effectively in planning preventive communication campaigns.

Site-specific ecological risk assessment of metal-contaminated soils based on the TRIAD approach.

Ecological risk assessment based on scientific data is crucial for understanding causal relationships between chemical pollution and environmental risks. Simultaneously, a balance is required between socioeconomic factors and scientific evidence. The TRIAD approach, which incorporates three lines of evidence (LoE)-chemical (Chem-LoE), ecotoxicological (Ecotox-LoE), and ecological (Eco-LoE)-was applied in five sites of an abandoned mine for site-specific soil ecological risk assessment (SERA). In combination, the three LoEs showed that two sites had extremely high risks, one site had moderate risk, and the other site had low risk. At all sites, Chem-LoE exhibited high-integrated risk values. In Ecotox-LoE and Eco-LoE, some species were not affected despite high metal concentrations in the soil samples collected from the sites, indicating that the bioavailability of metals differed according to the physiochemical properties of the soil medium. This study is significant as multiple analyses were performed considering ecosystem structure to reduce uncertainty in SERA. The results provide information to support effective decision-making risk management to protect the soil ecosystem. Moreover, these findings will be useful in establishing policies and priorities for soil risk management.

Evaluation of residual level and distribution characteristics of organochlorine pesticides in agricultural soils in South Korea.

In this study, we evaluated the residual levels and distribution characteristics of 10 organochlorine pesticides (OCPs) in the soil of agricultural lands (paddy field, vegetable field, and orchard, n = 150) nationwide. As a result of the investigation, 3-10 types of OCPs were detected in combination in 144 locations (96%) of the total 150 locations. Among those OCPs, dichlorodiphenyltrichloroethane (DDT) and endosulfan were detected in high amounts. The geometric mean concentrations of DDT and endosulfan were ∑DDT 0.31 ng g-1 (ND-2187.18 ng g-1) and ∑endosulfan 0.33 ng g-1 (ND-312.68 ng g-1), respectively. OCP concentrations were generally higher in orchards than in other agricultural fields due to a large amount of pesticides used. DDT in two orchards was detected at 2187.18 ng g-1 and 1061.78 ng g-1, exceeding the levels stated in the Canadian DDT Soil Quality Guidance (SQG, 700 ng g-1). The result of applying the isomer ratio to evaluate the recent or past use of DDT, endosulfan, and hexachlorocyclohexane (HCH), which had a high detection rate, showed that it could be attributable to historical usage. DDT and HCH showed statistically similar residual distribution patterns; however, endosulfan did not show a clear correlation with DDT and HCH due to the effect of its recent use. Thus, the results of evaluation of OCP residual levels and distribution characteristics can be used for the soil management policy of OCPs in agricultural lands in Korea.

Inhibition of glucose transport synergizes with chemical or genetic disruption of mitochondrial metabolism and suppresses TCA cycle-deficient tumors.

Efforts to target glucose metabolism in cancer have been limited by the poor potency and specificity of existing anti-glycolytic agents and a poor understanding of the glucose dependence of cancer subtypes in vivo. Here, we present an extensively characterized series of potent, orally bioavailable inhibitors of the class I glucose transporters (GLUTs). The representative compound KL-11743 specifically blocks glucose metabolism, triggering an acute collapse in NADH pools and a striking accumulation of aspartate, indicating a dramatic shift toward oxidative phosphorylation in the mitochondria. Disrupting mitochondrial metabolism via chemical inhibition of electron transport, deletion of the malate-aspartate shuttle component GOT1, or endogenous mutations in tricarboxylic acid cycle enzymes, causes synthetic lethality with KL-11743. Patient-derived xenograft models of succinate dehydrogenase A (SDHA)-deficient cancers are specifically sensitive to KL-11743, providing direct evidence that TCA cycle-mutant tumors are vulnerable to GLUT inhibitors in vivo.

Characteristic distribution patterns of perfluoroalkyl substances in soils according to land-use types.

In this study, a nationwide monitoring of perfluoroalkyl substances (PFAS) in soils was conducted for various land-use types around South Korea, such as industrial complexes, landfills, farmlands, mountains, and woodlands. The ∑15PFAS concentrations in soil samples ranged from 0.175 to 11.7 ng/g dry weight (dw) (median 2.39 ng/g dw). The soil samples from industrial complexes (0.346-11.7 ng/g dw; median 3.25 ng/g dw) and landfills (0.504-10.4 ng/g dw; median 2.13 ng/g dw) had higher median ∑15PFAS concentrations than other regions (0.175-3.01 ng/g dw; median 1.15 ng/g dw). In the soil samples from industrial areas, the ∑15PFAS concentrations ranged from 3.11 to 11.7 ng/g dw (median 6.32 ng/g dw) from the textile industries, 3.26-8.74 ng/g dw (median 6.20 ng/g dw) from the metal industries, 1.53-5.71 ng/g dw (median 3.38 ng/g) from the chemical industries, and 0.346-3.48 ng/g dw (median 2.39 ng/g dw) from the electronics industries. Perfluorooctane sulfonate (PFOS), perfluorooctanoic acid (PFOA), and long chain perfluorinated carboxylic acids (PFCAs) between C9 and C12 were generally dominant in these soils. In particular, PFOS, perfluorohexane sulfonate (PFHxS), and PFCAs with ≤ C6 were more frequently found in soils from industrial complexes than from other land-use types. On the other hand, PFCAs between C7 and C12 had relatively high detection frequencies regardless of the soil type.

Identification of Metal Contamination Sources and Evaluation of the Anthropogenic Effects in Soils near Traffic-Related Facilities.

Traffic-related facilities typically have much lower metal emissions than other sources; however, they can be numerous and widespread as well. Subdividing pollution sources is necessary to assess soil contamination characteristics and identify sources according to the contamination cause. Anthropogenic contamination by metals was quantitatively determined using contamination factor (Cf) and evaluated using multivariate analysis. More than half of the concentrations for Zn, Pb, and Cu in soils were higher than that in the natural background (NB). Cf of metals was, in decreasing order, Zn > Pb = Cu > Ni = As. Zn, Pb, and Cu were identified as anthropogenic contaminants in correlation analysis. Principal component analysis showed that the two main contamination causes were coarse particles from the maintenance or crushing activities of vehicles and nonexhaust/exhaust emissions. Clusters were classified according to those two anthropogenic and lithogenic causes and included Group I (Zn, Pb, and Cu in garages, auto repair shops, and auto salvage yards), Group II (Zn, Pb, and Cu in parking lots, driving schools, and roadsides), and Group III (As and Ni with high lithogenic properties). Anthropogenic input and sources of soil contamination by metals in traffic-related facilities were appropriately estimated through the combination of Cf and multivariate analysis.

Bayesian spatial modeling of disease risk in relation to multivariate environmental risk fields.

The relationship between exposure to environmental chemicals during pregnancy and early childhood development is an important issue that has a spatial risk component. In this context, we have examined mental retardation and developmental delay (MRDD) outcome measures for children in a Medicaid population in South Carolina and sampled measures of soil chemistry (e.g. As, Hg, etc.) on a network of sites that are misaligned to the outcome residential addresses during pregnancy. The true chemical concentration at the residential addresses is not observed directly and must be interpolated from soil samples. In this study, we have developed a Bayesian joint model that interpolates soil chemical fields and estimates the associated MRDD risk simultaneously. Having multiple spatial fields to interpolate, we have considered a low-rank Kriging method for the interpolation that requires less computation than the Bayesian Kriging. We performed a sensitivity analysis for a bivariate smoothing, changing the number of knots and the smoothing parameter. These analyses show that a low-rank Kriging method can be used as an alternative to a full-rank Kriging, reducing the computational burden. However, the number of knots for the low-rank Kriging model needs to be selected with caution as a bivariate surface estimation can be sensitive to the choice of the number of knots.

Benzimidazole- and indole-substituted 1,3'-bipyrrolidine benzamides as histamine H3 receptor antagonists.

Using a focused screen of biogenic amine compounds we identified a novel series of H(3)R antagonists. A preliminary SAR study led to reduction of MW while increasing binding affinity and potency. Optimization of the physical properties of the series led to (S)-6n, with improved brain to plasma exposure and efficacy in both water intake and novel object recognition models.