ABSTRACT
Human pluripotent stem cells (hPSCs) are thought to be a promising cell-source solution for regenerative medicine due to their indefinite proliferative potential and ability to differentiate to functional somatic cells. However, issues remain with regard to achieving reproducible differentiation of cells with the required functionality for realizing human transplantation therapies and with regard to reducing the potential for bacterial or fungal contamination. To meet these needs, we have developed a closed-channel culture device and corresponding control system. Uniformly-sized spheroidal hPSCs aggregates were formed inside wells within a closed-channel and maintained continuously throughout the culture process. Functional islet-like endocrine cell aggregates were reproducibly induced following a 30-day differentiation protocol. Our system shows an easily scalable, novel method for inducing PSC differentiation with both purity and functionality.
Subject(s)
Batch Cell Culture Techniques/instrumentation , Cell Differentiation/physiology , Cell Separation/instrumentation , Islets of Langerhans/cytology , Lab-On-A-Chip Devices , Pluripotent Stem Cells/cytology , Batch Cell Culture Techniques/methods , Cell Separation/methods , Cells, Cultured , Cellular Reprogramming Techniques/instrumentation , Cellular Reprogramming Techniques/methods , Equipment Design , Equipment Failure Analysis , HumansABSTRACT
We have achieved a high compression ratio by stimulated Brillouin scattering (SBS) consisting of two long cells. A 13-ns Nd:YAG laser pulse was temporally compressed to about 160-ps phase-conjugated pulse in heavy fluorocarbon FC-40 liquid at a 1064 nm wavelength. The maximum reflectivity of SBS process was over 80 % without an optical damage. The compressed pulse brightness was about 65-fold higher than that of the incident pulse.