RTA dh404 Induces Cell Cycle Arrest, Apoptosis, and Autophagy in Glioblastoma Cells.

RTA dh404 is a novel synthetic oleanolic acid derivative that has been reported to possess anti-allergic, neuroprotective, antioxidative, and anti-inflammatory properties, and exerts therapeutic effects on various cancers. Although CDDO and its derivatives have anticancer effects, the actual anticancer mechanism has not been fully explored. Therefore, in this study, glioblastoma cell lines were exposed to different concentrations of RTA dh404 (0, 2, 4, and 8 µM). Cell viability was evaluated using the PrestoBlue™ reagent assay. The role of RTA dh404 in cell cycle progression, apoptosis, and autophagy was analyzed using flow cytometry and Western blotting. The expression of cell cycle-, apoptosis-, and autophagy-related genes was detected by next-generation sequencing. RTA dh404 reduces GBM8401 and U87MG glioma cell viability. RTA dh404 treated cells had a significant increase in the percentage of apoptotic cells and caspase-3 activity. In addition, the results of the cell cycle analysis showed that RTA dh404 arrested GBM8401 and U87MG glioma cells at the G2/M phase. Autophagy was observed in RTA dh404-treated cells. Subsequently, we found that RTA dh404-induced cell cycle arrest, apoptosis, and autophagy were related to the regulation of associated genes using next-generation sequencing. Our data indicated that RTA dh404 causes G2/M cell cycle arrest and induces apoptosis and autophagy by regulating the expression of cell cycle-, apoptosis-, and autophagy-related genes in human glioblastoma cells, suggesting that RTA dh404 is a potential drug candidate for the treatment of glioblastoma.

The effects of a magic-based intervention on self-esteem, depressive symptoms, and quality of life among community-dwelling older adults: a randomised controlled trial.

BACKGROUND: Magic-based programs have been utilised to enhance well-being across various health aspects. However, there is a lack of studies on whether performing magic tricks can provide mental health benefits for older adults living in the community. Therefore, this study aims to investigate the effects of a magic-based intervention program on self-esteem, depressive symptoms, and quality of life (QOL), and to examine the relationship between these factors in older adults. METHODS: Thirty-eight participants, aged 60-90 years, were randomly assigned to either a magic intervention group or a control group. The magic intervention program, tailored for older adults, was conducted for 90 min, twice weekly, over 6 weeks. The Rosenberg Self-Esteem Scale (RSE), the 15-item Geriatric Depression Scale (GDS-15), and the World Health Organization Quality of Life-BREF scores were measured and analyzed in both groups before and after the intervention. RESULTS: The magic-based intervention significantly increased self-esteem and reduced depressive symptoms in older adults, with large effect sizes. However, no significant impact on QOL was observed. Additionally, no significant correlation was found between the improvement in self-esteem and the reduction in depressive levels. Despite this, a moderate but significant negative correlation was detected between the post-intervention scores of RSE and GDS-15 in the magic intervention group. CONCLUSIONS: The study demonstrated that the magic intervention program was beneficial in promoting mental health in community-dwelling older adults. Implementing magic programs in communities appears to be an effective approach to enhance self-esteem and alleviate depressive symptoms in the older population.

Dual pretreatment of mixing H2O2 followed by torrefaction to upgrade spent coffee grounds for fuel production and upgrade level identification of H2O2 pretreatment.

Biochar is a carbon-neutral solid fuel and has emerged as a potential candidate to replace coal. Meanwhile, spent coffee grounds (SCGs) are an abundant and promising biomass waste that could be used for biochar production. This study develops a biochar valorization strategy by mixing SCGs with hydrogen peroxide (H2O2) at a weight ratio of 1:0.75 to upgrade SCG biochar. In this dual pretreatment method, the H2O2 oxidative ability at a pretreatment temperature of 105 °C contributes to an increase in the higher heating value (HHV) and carbon content of the SCG biochars. The HHV and carbon content of biochar increase by about 6.5% and 7.8%, respectively, when compared to the unpretreated one under the same conditions. Maximized biochar's HHV derived via the Taguchi method is 30.33 MJkg-1, a 46.9% increase compared to the raw SCG, and a 6.5% increase compared to the unpretreated SCG biochar. The H2O2 concentration is 18% for the maximized HHV. A quantitative identification index of intensity of difference (IOD) is adopted to evaluate the contributive level of H2O2 pretreatment in terms of the HHV and carbon content. IOD increases with increasing H2O2 pretreatment temperature. Before torrefaction, SCGs' IOD pretreated at 50 °C is 1.94%, while that pretreated at 105 °C is 8.06%. This is because, before torrefaction, H2O2 pretreatment sufficiently weakens SCGs' molecular structure, resulting in a higher IOD value. The IOD value of torrefied SCGs (TSCG) pretreated at 105 °C is 10.71%, accounting for a 4.59% increase compared to that pretreated at 50 °C. This implies that TSCG pretreated by H2O2 at 105 °C has better thermal stability. For every 1% increase in IOD of TSCG, the carbon content of the biochar increases 0.726%, and the HHV increases 0.529%. Overall, it is demonstrated that H2O2 is a green and promising pretreatment additive for upgrading SCG biochar's calorific value, and torrefied SCGs can be used as a potential solid fuel to approach carbon neutrality.

Impact of a magic recreation program on older adults with minor depressive symptoms in a long-term care facility: A pilot randomized controlled trial.

OBJECTIVES: To evaluate a magic recreation program to reduce depressive symptoms in institutionalized older adults. METHODS: We conducted a pilot randomized controlled trial in which participants were assigned to either a magic group (n = 6) or a control group with usual activities (n = 6). The magic group received a 6-week magic recreation program. The data were analyzed by generalized estimating equations in terms of intention-to-treat analysis. A sensitivity analysis was conducted by examining the complete case analysis. RESULTS: The magic recreation program significantly improved the scores of Patient Health Questionnaire-9 in the magic group (Wald χ 2 = 8.816, p = 0.004, Cohen's  d  = 1.51, power = 0.9968). The results of the sensitivity analysis were consistent with the results of primary analysis. CONCLUSIONS: The 6-week magic recreation program reduced depressive symptoms among institutionalized older adults with minor depressive symptoms.

In-Situ Template Formation Strategy for the Preparation of Nitrogen Doped Carbon Nanocage with Graphitic Shell as Electrode Material for Supercapacitor.

Nitrogen doped carbon nanocage with graphitic shell (NGCS) was fabricated through in-situ solid reaction between calcium acetate and dicyandiamide in an inert atmosphere followed by acid etching. The role played by the calcium acetate (Ca(Ac)2) and dicyandiamide (DCD) during the synthesis process is one-stone-two-birds. Calcium acetate plays multiple functions: template agent, graphitization catalyst, and carbon source. Dicyandiamide can be considered as the nitrogen sources and the chemical reaction agent that can be reacted with calcium acetate to form it into CaCN2. The NGCS obtained at 800 °C has a specific surface area of 420 m2/g and nitrogen content of 8.87 at%. The excellent electrochemical performance can be attributed to the combination effects of porous structure, nitrogen doping and graphitized nanocage shell of NGCS electrode. The hollow structure serves as the reservoir for fast electrolyte ion supplement. Nitrogen groups not only improve the wettability of interfaces between carbon surface and electrolyte, but also generate extra pseudocapacitance through redox reaction. The graphitic carbon nanocage shell can enhance the conductivity and facilitates the fast charge transfer. At a current density of 0.5 A/g, the specific capacitance of the NGCS-800 electrode is 215 F/g. Furthermore, the NGCS-800 electrode exhibits excellent rate capability (80% capacitance retention at 10 A/g) and outstanding cycling stability (96.89% capacitance retention after 5000 cycles). These intriguing results demonstrate that nitrogen doped carbon with graphitic shell will be highly promising as electrode materials for supercapacitors and other energy storage and conversation applications.

Assessing Pain Intensity Using Photoplethysmography Signals in Chronic Myofascial Pain Syndrome.

BACKGROUND: Efficacy of pain assessment is the basis for effective therapy. Clinically, assessing pain is by subjective scale, but these methods have some shortcomings. Therefore, studies have been conducted on assessment of pain using physiological signals. Photoplethysmography (PPG) signals provide much information about the cardiovascular system. PPG-derived parameters (PPG parameters) reflect nociceptive stimulation, and obtain an approximation of the R-R interval from the PPG period. The aim of this study was to evaluate PPG signals for assessment of pain intensity in chronic myofascial pain syndrome (MPS) patients. METHODS: This study recruited 37 patients with chronic MPS; all of them were treated with electrotherapy and thermotherapy. The difference between pre- and post-therapy PPG parameters, and the correlation between pulse rate variability (PRV) and heart rate variability (HRV) were determined. We also obtained patients' pain intensity scores by visual analog scale, visual rating scale, and Wong-Banker face pain rating scale. RESULTS: Photoplethysmography and PRV/HRV parameters showed significant differences between pre- and post-treatment. The variation trend of PRV was similar with HRV in heart rate, R-R interval, low frequency, high frequency, and LF/HF; in addition, a high correlation between the parameters was observed either in pre- or post-therapy. PPG parameters indicated increased sympathetic tone. CONCLUSION: The results of the study indicated that PRV substituted for HRV in assessment of pain intensity in chronic MPS reflected parasympathetic nervous tone increase, and PPG parameters might reflect stress stimulation on skin.

Use of Heart Rate Variability and Photoplethysmograph-Derived Parameters as Assessment Signals of Radiofrequency Therapy Efficacy for Chronic Pain.

BACKGROUND: Radiofrequency therapy (RFT) generates molecular motion and produces heat and electromagnetic effects on tissues, which attenuate pain sensation and thereby relieve pain. This study was to observe the altering trend of physiological parameters after RFT for chronic cervical or lumbar pain. METHODS: This study recruited 66 patients with chronic cervical or lumbar pain and recorded their physiological parameters before and after RFT using heart rate variability (HRV) and photoplethysmography (PPG) to explore the feasibility of RFT efficacy assessment. RESULTS: The patients' visual analog scale scores significantly decreased after RFT and the HRV parameters that represented parasympathetic activity significantly changed (HR decreased, and R-R interval and low- and high-frequency power increased significantly). Meanwhile, the PPG parameters that represented sympathetic activity also increased (PPG amplitude and autonomic nervous system state significantly decreased). CONCLUSIONS: This study showed significant efficacy of RFT in patients with chronic cervical or lumbar pain. The changes of HRV and PPG parameters may explain part of the mechanisms of RFT.

SnFe2 O4 Nanocrystals as Highly Efficient Catalysts for Hydrogen-Peroxide Sensing.

SnFe2 O4 nanocrystals (NC), prepared with a simple one-step carrier-solvent-assisted interfacial reaction process, were developed as highly efficient catalysts for hydrogen peroxide sensing. These NCs, with a size of around 7 nm, served as the sensing catalyst and were decorated onto the pore surfaces of a porous fluorine-doped tin oxide (PFTO) host electrode, prepared from commercial FTO glass with a simple anodic treatment, to form the sensing electrode for hydrogen peroxide. The SnFe2 O4 NCs-loaded PFTO electrode exhibited an ultra-high sensitivity of 1027 mA m(-1) cm(-2) toward hydrogen peroxide, outperforming Pt NCs-loaded PFTO electrodes. The SnFe2 O4 NCs-loaded PFTO electrode proved a promising relatively low cost, high performance sensing electrode for hydrogen peroxide.

Photoplethysmography variability as an alternative approach to obtain heart rate variability information in chronic pain patient.

Heart rate variability (HRV) is a well-known method for the assessment of autonomic nervous function of the heart. Previous study suggested that pulse rate variability (PRV) determined by photoplethysmography could be used instead of HRV to more simply assess autonomic nervous function. However, most research studies included healthy subjects. Thus, the aim of this study was to investigate the feasibility for PRV as a surrogate index for patients with chronic pain. This study investigated the correlation coefficient (by Pearson correlation) and agreement (by Bland-Altman analysis) between PRV and HRV in chronic pain patients in the clinical setting. The results showed high significant correlations (p < 0.001, r > 0.86) between all the HRV and PRV parameters and good agreements (ratio < 0.1) between the parameters in terms of HR, mean RR, VLF, LF, nLF, nHF, and SD1/SD2. Our study suggests that HRV can also be reliably estimated using the photoplethysmography-based PP interval in elderly patients with chronic pain.

On practicing magicine, from wonder to care: A systematic review of studies that apply magic in healthcare.

RATIONALE: Magic, traditionally perceived as entertainment, has been increasingly employed in healthcare to enhance health and well-being. Despite its potential benefits across various dimensions of health, including biological, psychological, and social, a comprehensive review highlighting its broad applications in healthcare remains unexplored. OBJECTIVE: This study aims to explore the diverse uses of magic within healthcare, progressing from entertainment to integral medical care, termed "magicine." METHODS: This systematic review adopted a narrative synthesis approach, and an extensive database search was conducted including Embase Classic & Embase, MEDLINE (Ovid), Scopus, the Cochrane Collaboration Central Register of Controlled Clinical Trials, Cochrane Systematic Reviews, and CINAHL (EBSCOhost), from the earliest records to 22 June 2023. Potential applications of magic in healthcare were explored with an unrestricted search strategy. A quality assessment was conducted using the Mixed Methods Appraisal Tool. (Registration: PROSPERO number CRD42023417122.) RESULTS: This review identified 82 journal articles, including 11 randomized controlled trials, four quasi-experimental designs, 10 pre-experimental designs, five qualitative studies, three mixed methods studies, two observational studies, five review articles, and 42 commentaries. The review resulted in the conception of "magicine ennead" - nine diverse areas where magic can be applied in healthcare including physical rehabilitation, cognitive training, psychotherapy, humor therapy, distraction therapy, social skills, health education, doctor-patient relationships, and surgical techniques. These applications demonstrate the potential of magic to enhance health outcomes for the general population and improve the clinical practice of healthcare professionals. CONCLUSIONS: Magic in healthcare shows potential for varied applications, and a deeper understanding of these applications could lead to optimized and cost-efficient intervention programs. Given the heterogeneity and varied methodological quality of the current research, future studies necessitate the adoption of rigorous designs with active controls.

JSI-124 Induces Cell Cycle Arrest and Regulates the Apoptosis in Glioblastoma Cells.

Cucurbitacin I (JSI-124), derived from Cucurbitaceae, has shown the potential to induce apoptosis and cell cycle arrest in some cancer cells. However, the effect of JSI-124 on glioblastoma multiforme (GBM) cell cycle and apoptosis is still unclear. Our investigation revealed that JSI-124 effectively reduced cell viability in GBM cells, leading to apoptosis and increased caspase-3 activity. Intriguingly, JSI-124 caused the accumulation of G2/M phase to regulate cell cycle, confirmed by MPM-2 staining and increased protein synthesis during mitosis by mitotic index analysis. Western blot analysis found that JSI-124 affected the progression of G2/M arrest by downregulating the CDK1 and upregulating the cyclinB1, suggesting that JSI-124 disrupted the formation and function of the cyclin B1/CDK1 complex in GBM8401 and U87MG cells. However, we found the JSI-124-regulated cell cycle G2/M and apoptosis-relative gene in GBM8401 and U87MG cells by NGS data analysis. Notably, we found that the GBM8401 and U87MG cells observed regulation of apoptosis and cell-cycle-related signaling pathways. Taken together, JSI-124 exhibited the ability to induce G2/M arrest, effectively arresting the cell cycle at critical stages. This arrest is accompanied by the initiation of apoptosis, highlighting the dual mechanism of action of JSI-124. Collectively, our findings emphasize that JSI-124 holds potential as a therapeutic agent for GBM by impeding cell cycle progression, inhibiting cell proliferation, and promoting apoptosis. As demonstrated by our in vitro experiments, these effects are mediated through modulation of key molecular targets.

Sulforaphane-Induced Cell Mitotic Delay and Inhibited Cell Proliferation via Regulating CDK5R1 Upregulation in Breast Cancer Cell Lines.

Our research has revealed that sulforaphane (SFN) has chemopreventive properties and could be used in chemotherapy treatments. Further investigation is needed to understand the mechanisms behind sulforaphane's (SFN) antitumor activity in breast adenocarcinoma, as observed in our studies. This research looked into the effects of SFN on mitosis delay and cell cycle progression in MDA-MB-231 and ZR-75-1 cells, two types of triple-negative breast cancer adenocarcinoma.The proliferation of the cancer cells after SFN exposure was evaluated using MTT assay, DNA content and cell cycle arrest induction by flow cytometry, and expressions of cdc25c, CDK1, cyclin B1 and CDK5R1 were assessed through qRT-PCR and Western blot analysis. SFN was found to inhibit the growth of cancer cells. The accumulation of G2/M-phase cells in SFN-treated cells was attributed to CDK5R1. The disruption of the CDC2/cyclin B1 complex suggested that SFN may have antitumor effects on established breast adenocarcinoma cells. Our findings suggest that, in addition to its chemopreventive properties, SFN could be used as an anticancer agent for breast cancer, as it was found to inhibit growth and induce apoptosis of cancer cells.

Spent coffee ground torrefaction for waste remediation and valorization.

Spent coffee grounds (SCGs) are a noticeable waste that may cause environmental pollution problems if not treated appropriately. Torrefaction is a promising low-temperature carbonization technique to achieve waste remediation, recovery, and circular bioeconomy efficiently. This study aims to maximize lipids retained in thermally degraded SCGs, thereby upgrading their fuel quality to implement resource sustainability and availability. This work also analyzes the lipid contribution to biochar's calorific value under various carbonization temperatures and times. Torrefaction can retain 11-15 wt% lipids from SCG, but the lipid content decreases when the pyrolysis temperature is higher than 300 °C. Extracted lipid content consisting of fatty acids echoed the results of diesel adsorption capacity. The lipid content in the biochar from SCG torrefied at 300 °C for 30 min is 11.00 wt%, and its HHV is 28.16 MJ kg-1. In this biochar, lipids contribute about 14.84% of the calorific value, and the other carbonized solid contributes 85.16%. On account of the higher lipid content in the biochar, it has the highest diesel adsorption amount per unit mass, with a value of 1.66 g g-1. This value accounts for a 22.1% improvement compared to its untorrefied SCG. Accordingly, torrefaction can sufficiently remediate SCG-derived environmental pollution. The produced biochar can become a spilled oil adsorbent. Furthermore, oil-adsorbed biochar (oilchar) is a potential solid fuel. In summary, SCG torrefaction can simultaneously achieve pollution remediation, waste valorization, resource sustainability, and circular bioeconomy.

Construction and demolition waste as a high-efficiency advanced process for organic pollutant degradation in Fenton-like reaction to approach circular economy.

The Fenton-like reaction is a promising organic wastewater treatment reaction among advanced oxidation processes (AOP), which has emerged to replace the conventional Fenton reaction. Recycled construction and demolition waste (CDW), which is porous and rich in iron, manganese, and magnesium, can be reused as a Fenton-like catalyst. This study proposes an AOP wastewater treatment strategy using recycled porous CDW mixed with hydrogen peroxide (H2O2) to decompose methylene blue (MB) wastewater. According to the apparent first-order rate (Kapp) of 10 ppm MB adsorption, CDW-3, having the highest specific surface area, also has the highest Kapp of 0.23 min-1 g-1. The optimized conditions recommended by the Taguchi method include a 0.3 g mL-1 CDW-3 concentration, a 0.254 g mL-1 H2O2 concentration, and 10 ppm MB, resulting in an about 2.01 min-1Kapp value. In addition, MB concentration is observed as the most influential factor for Kapp, which decreases with increasing MB concentration and is about 0.62 min-1 at 1000 ppm MB. Repeating the Fenton-like reaction five times at 100 p.m. MB using the same CDW-3, the Kapp is about 0.64 min-1, which is 86% of the initial run. The synergistic effect index (ξ) is defined to quantify the level of interaction between CDW and H2O2, which produces free radicals during the Fenton-like process. The ξ of CDW-3 is about 2.16. Overall, it is demonstrated that CDW is a promising catalyst for Fenton-like reactions, and the synergistic effect index (ξ) can be used as a reference index to evaluate the catalytic generation of free radicals between the catalyst and H2O2.

Multi-objective operation optimization of spent coffee ground torrefaction for carbon-neutral biochar production.

Many energy-intensive processes are employed to enhance biomass fuel properties to overcome the difficulties in utilizing biomass as fuel. Therefore, energy conservation during these processes is crucial for realizing a circular bioeconomy. This study develops a newly devised method to evaluate SCG biochars' higher heating value (HHV) and predict moisture content from power consumption. It is found that the increasing rates of HHV immediately follow decreases in power consumption, which could be used to determine the pretreatment time for energy conservation. The non-dominated sorting genetic algorithm II (NSGA-II) maximizes SCG biochar's HHV while minimizing energy consumption. The results show that producing SCG biochar with 23.98 MJ∙kg-1 HHV requires 20.042 MJ∙kg-1, using a torrefaction temperature of 244 °C and torrefaction time of 27 min and 43 sec. Every kilogram of biochar with an energy yield of 85.93 % is estimated to cost NT$ 12.21.

A Novel Synthetic Oleanolic Acid Derivative Inhibits Glioma Cell Proliferation by Regulating Cell Cycle G2/M Arrest.

2-Cyano-3,12-dioxooleana-1,9(11)-dien-28-oic acid-9,11-dihydro-trifluoroethyl amide (CDDO-dhTFEA) has antioxidant and anti-inflammatory activities; however, whether CDDO-dhTFEA has anticancer effects is unclear. The objective of this research was to investigate the possibility of CDDO-dhTFEA as a potential cancer-fighting treatment in glioblastoma cells. Our experiments were performed on U87MG and GBM8401 cells, and we found that CDDO-dhTFEA was effective in reducing cell proliferation in both cell lines, in a manner that was dependent on both time and concentration. Additionally, we observed that CDDO-dhTFEA had a significant impact on the regulation of cell proliferation, which was evident in the increase in DNA synthesis that was observed in both cell types. CDDO-dhTFEA induced G2/M cell cycle arrest and mitotic delay, which may be associated with the inhibition of proliferation. Treatment with CDDO-dhTFEA led to cell cycle G2/M arrest and inhibited proliferation of U87MG and GBM8401 cells by regulating G2/M cell cycle proteins and gene expression in GBM cells in vitro.

An overview of commercialization and marketization of thermoelectric generators for low-temperature waste heat recovery.

According to statistics, low-temperature waste heat below 300°C accounts for more than 89% of industrial waste heat. If the waste heat is not recycled, a large amount of low-temperature waste heat will be released into the atmosphere, thereby exacerbating global warming and posing a significant threat to human survival. Although the power generation efficiency of solid-state thermoelectric generation technology is lower than the organic Rankine cycle, it only requires a smaller construction area, which increases its market acceptance, applicability, and penetration. Especially in the pursuit of net-zero emissions by global companies, the importance of low-temperature waste heat recovery and power generation is even more prominent. The current thermoelectric conversion efficiency of commercial thermoelectric chips is about 5%. Power generation cost, thermoelectric conversion efficiency, and energy use efficiency are highly correlated with the commercialization of solid-state thermoelectric technology. This research shares five practical waste heat power generation cases commercialized by recycling three heat sources. It also points out the three significant challenges facing the commercialization of power generation from low-temperature waste heat recovery. This study analyzes 2,365 TEG patents submitted by 28 companies worldwide to determine the basic technology for realizing waste heat recovery through TEG and explore the potential commercialization of related waste heat recovery products. The future challenge for the large-scale commercialization of solid-state thermoelectric technology is not technological development but financial incentives related to changes in international energy prices and subsidies that promote zero carbon emissions.

The Effects of a Magic Intervention Program on Cognitive Function and Neurocognitive Performance in Elderly Individuals With Mild Cognitive Impairment.

Objectives: Cognitive training is one of the management options for elderly individuals who suffer from mild cognitive impairment (MCI) and an effective way to improve executive function. This study aimed to evaluate the effectiveness of a magic intervention program as a method of cognitive training in improving cognitive function and neurocognitive performance in this group. Methods: Twenty-four participants aged 60-80 years with MCI were recruited and randomly assigned to a magic intervention group or a control group. The magic intervention group received a 6-week magic intervention program. The primary endpoints were the scores for the cognitive assessment tests [e.g., Mini-Mental State Examination (MMSE) and Montreal Cognitive Assessment (MoCA)] for general cognitive function. The secondary endpoints were the behavioral [e.g., accuracy and reaction times] and the electroencephalographic [e.g., event-related potential (ERP) P3 amplitudes] performance during the Flanker task to assess attention and inhibitory control. All variables were measured before and after the magic intervention. Results: The results showed that the 6-week magic intervention significantly improved the MoCA scores in the cognitive assessment tests although no significant pre-post intervention difference was observed in the MMSE scores. In terms of neurocognitive performance, the magic intervention had significantly positive effects on the accuracy, reaction times, and P3 amplitudes when performing the Flanker task. Conclusion: The results of the present study showed that the 6-week magic intervention had beneficial effects on the cognitive and electrophysiological performance in the elderly subjects with MCI. For such a group, lifestyle intervention programs that encourage participation such as the magic practice and performance may be a viable suggestion to prevent the progression of MCI to Alzheimer's disease.

Corrigendum: The Effects of a Magic Intervention Program on Cognitive Function and Neurocognitive Performance in Elderly Individuals With Mild Cognitive Impairment.

[This corrects the article DOI: 10.3389/fnagi.2022.854984.].

Radix Glycyrrhizae Preparata Induces Cell Cycle Arrest and Induced Caspase-Dependent Apoptosis in Glioblastoma Multiforme.

Glioblastoma multiforme (GBM) is a highly aggressive and devastating brain tumor characterized by poor prognosis and high rates of recurrence. Despite advances in multidisciplinary treatment, GBM constinues to have a poor overall survival. The Radix Glycyrrhizae Preparata (RGP) has been reported to possess anti-allergic, neuroprotective, antioxidative, and anti-inflammatory activities. However, it not clear what effect it may have on tumorigenesis in GBM. This study demonstrated that RGP reduced glioma cell viability and attenuated glioma cell locomotion in GBM8401 and U87MG cells. RGP treated cells had significant increase in the percentage of apoptotic cells and rise in the percentage of caspase-3 activity. In addition, the results of study's cell cycle analysis also showed that RGP arrested glioma cells at G2/M phase and Cell failure pass the G2 checkpoint by RGP treatment in GBM8401 Cells. Based on the above results, it seems to imply that RGP activated DNA damage checkpoint system and cell cycle regulators and induce apoptosis in established GBM cells. In conclusion, RGP can inhibit proliferation, cell locomotion, cell cycle progression and induce apoptosis in GBM cells in vitro.