Examining Coping Strategies and Mental Health during the COVID-19 Pandemic: Evidence for the Protective Role of Problem-Focused Coping.

Early in the COVID-19 pandemic, studies documented a marked decline in mental health and well-being when compared with prepandemic levels. This study examined how different coping styles were associated with anxiety, depression, and sleep problems among U.S. adults March-April 2020 during the COVID-19 pandemic. Data from 535 U.S. adults across three time points were analyzed using longitudinal multilevel logistic and linear regression modeling. Avoidant-emotional coping was associated with greater odds of experiencing anxiety (OR = 1.65, p < .001). Both avoidant- and active-emotional coping were associated with greater odds of experiencing depression (OR = 1.67, p < .001, and OR = 1.09, p = .022, respectively) and sleep problems (b = 0.05, p < .001, and b = 0.01, p = .005, respectively). Alternatively, problem-focused coping was associated with lower odds of depression (OR = 0.86, p < .001). Results converge with previous evidence suggesting the perniciousness of avoidant-emotional coping during the pandemic, but also underscore that problem-focused coping strategies may represent one important source of resilience for adults to adapt despite such challenges.

Implementing a Text Messaging Intervention to Engage Fathers in Home Visiting.

PURPOSE: This "From the Field" article reports on the Text4Dad text messaging intervention designed to engage fathers in home visiting. We introduce implementation process components from our pilot study across three Healthy Start home visitation sites. DESCRIPTION: Three Fatherhood Community Health Workers (F-CHWs) and three fathers from one Text4Dad site were interviewed. Using content analysis, we examined the experiences of F-CHWs who implemented Text4Dad and program participants who used Text4Dad. ASSESSMENT: Results highlighted five implementation process components related to: (1) F-CHWs' use of Text4Dad and enrolling fathers; (2) F-CHWs' interactions with fathers, perceptions of Text4Dad content, and integration of Text4Dad into home visits with fathers; (3) training and technical assistance for F-CHWs; (4) father program participants' acceptability and usability of Text4Dad; and (5) fathers' barriers to interactive use of Text4Dad. CONCLUSION: The F-CHWs were able to successfully enroll fathers into Text4Dad. F-CHWs and fathers found Text4Dad content acceptable to their circumstances. Text4Dad technology was viewed as usable, with some limitations. F-CHWs experienced challenges accessing the Text4Dad platform while on home visits. Results suggested that F-CHWs did not use Text4Dad to facilitate interaction, and accordingly, fathers had a lower than anticipated response rate to texts sent by their F-CHWs. We conclude with future directions for improving the implementation of text messaging programs in community-based fatherhood programs.

Childhood maltreatment history and trauma-specific predictors of parenting stress in new fathers.

For new fathers, parenting stress is a risk factor for impaired early parenting and child maltreatment perpetration. Predictors of parenting stress, including fathers' own experiences of trauma, could be useful intervention targets to support new fathers. We aim to examine associations between new fathers' own histories of child maltreatment, and their perinatal mental health, relationships, and parenting stress. We recruited 298 first-time fathers for a survey that measured child maltreatment history, trauma sequelae including posttraumatic stress disorder (PTSD), major depressive disorder (MDD), interpersonal reactivity, substance use, anger expression, coparenting quality, and parenting stress. On the Parenting Stress Index (PSI) (from 36 to 180), bivariate analysis demonstrated that new fathers who experienced child maltreatment (n = 94) had significantly higher parenting stress (x̅ = 85.3, σ = 18.7) than those who did not (n = 204; x̅ = 76.0, σ = 16.6; P < .000). Hierarchical linear regression modeling indicated that a child maltreatment history, PTSD, and MDD were significantly associated with parenting stress. The strongest predictors of parenting stress were coparenting quality and complex trauma sequelae-interpersonal reactivity and anger expression. Interventions to reduce fathers' parenting stress by targeting known mental health and relationship sequelae of maltreatment are promising avenues to breaking intergenerational transmission of child maltreatment and psychiatric vulnerability.

Para nuevos papás, el estrés de crianza es un factor de riesgo para la deficiente crianza temprana y para cometer maltrato infantil. Los factores de predicción del estrés de crianza, incluyendo las propias experiencias de trauma de los papás, pueden ser útiles metas de intervención para apoyar a los nuevos papás. Nos propusimos examinar las asociaciones entre las propias historias de maltrato de los nuevos papás, y su salud mental perinatal, relaciones y estrés de crianza. Reclutamos 298 papás primerizos para una encuesta que medía la historia de maltrato infantil, la secuela de trauma incluyendo el trastorno de estrés postraumático (PTSD), el trastorno depresivo serio (MDD), la reactividad interpersonal, el uso de sustancias, la expresión de ira, la calidad de la crianza compartida, así como el estrés de crianza. En el Índice de Estrés de Crianza (de 36-180), los análisis bivariantes demostraron que los nuevos papás que habían experimentado maltrato infantil (N = 94) tenían significativamente un mayor estrés de crianza (x̅ = 85.3, σ = 18.7) que aquellos que no habían tenido tal experiencia (N = 204; x̅ = 76.0, σ = 16.6; P<.000). El modelo de regresión lineal jerárquica indicó que una historia de maltrato infantil, PTSD y MDD estaban significativamente asociados con el estrés de crianza. Los más fuertes factores de predicción del estrés de crianza fueron la calidad de la crianza compartida y la compleja secuela de trauma-la reactividad interpersonal y la expresión de la ira. Las intervenciones para reducir el estrés de crianza de los papás por medio del enfoque en la salud mental conocida y las secuelas en la relación del maltrato son una vía prometedora para romper la transmisión intergeneracional del maltrato infantil y la vulnerabilidad siquiátrica.

Pour les nouveaux pères le stress de parentage est un facteur de risque pour le parentage précoce compromis et la perpétration de maltraitance de l'enfant. Les prédicteurs de stress de parentage, y compris les propres expériences de trauma des pères, pourraient être des cicles d'intervention utiles afin de soutenir les nouveaux pères. Nous nous sommes donné pour but d'examiner les liens entre le propre passé de maltraitance de l'enfant des nouveaux pères et leur santé mentale périnatale, leurs relations et le stress de parentage. Nous avons recruté 298 nouveaux pères (pères pour la première fois) pour un sondage mesurant l'histoire de la maltraitance de l'enfant, les séquelles de trauma y compris les troubles de stress post-traumatique (TSPT), les troubles dépressifs majeurs (MDD en anglais), la réactivité interpersonnelle, la toxicomanie, l'expression de colère et la qualité du co-parentage ainsi que le stress parental. Pour l'Index de Stress de Parentage (de 36-180), une analyse bivariée a montré que les nouveaux pères qui avaient fait l'expérience de maltraitance de l'enfance (N = 94) avaient un stress de parentage bien plus élevé (x̅ = 85,3, σ = 18,7) que ceux n'en ayant pas fait l'expérience (N = 204; x̅ = 76,0, σ = 16,6; P<,000). Un modèle de régression linéaire hiérarchique a indiqué qu'un passé de maltraitance de l'enfant, le TSPT et le MDD étaient fortement liés au stress de parentage. Les facteurs de prédiction les plus forts de stress de parentage étaient la qualité du co-parentage et les séquelles de trauma complexes - réactivité interpersonnelle et l'expression de la colère. Les interventions pour réduire le stress de parentage des pères en ciblant la santé mentale connue et les séquelles de maltraitance sont un chemin prometteur pour casser la transmission intergénérationnelle de la maltraitance de l'enfant et la vulnérabilité psychiatrique.

Technology-Facilitated Abuse Prevalence and Associations Among a Nationally Representative Sample of Young Men.

PURPOSE: We undertook a study to determine the prevalence and associations of technology-facilitated abuse (TFA)-insults, harassment, coercion, or threats carried out using digital tools such as smartphones and computers-among a US nationally representative sample of young men. METHODS: Analyses were based on 1,079 men aged 18 to 35 years who completed questionnaires during August and September of 2014 and reported ever having been in a romantic relationship. We used validated measures to assess demographics, health service use, mental health and substance use, and TFA delivered to and received from partners in the past year. We calculated survey-weighted descriptive statistics and conducted multinomial logistic regression analysis. RESULTS: Overall, 4.1% of men reported delivering TFA only, 8.0% receiving TFA only, and 25.6% both delivering and receiving TFA. Men were more likely to report only delivering TFA if they identified as Hispanic (adjusted odds ratio [AOR] = 2.72; 95% CI, 1.13 to 6.57), used marijuana (AOR = 1.31; 95% CI, 1.02 to 1.68), and used prescription opioids for nonmedical reasons (AOR 2.86; 95% CI, 1.48 to 5.54). Men were more likely to report only receiving TFA if they identified as Hispanic (AOR = 2.55; 95% CI, 1.01 to 6.43) and used prescription opioids for nonmedical reasons (AOR = 2.43; 95% CI, 1.34 to 4.39), whereas a primary care connection appeared protective (AOR = 0.43; 95% CI, 0.22 to 0.86). Men were more likely to report both delivering and receiving TFA if they identified as non-Hispanic Black (AOR = 2.83; 95% CI, 1.44 to 5.58), owned a smartphone (AOR = 1.80; 95% CI, 1.05 to 3.09), had ever had mental health care visits (AOR = 1.86; 95% CI, 1.16 to 2.98), misused alcohol (AOR = 1.10; 95% CI, 1.04 to 1.17), and used prescription opioids for nonmedical reasons (AOR = 1.79; 95% CI, 1.04 to 3.08). CONCLUSIONS: We found that TFA was prevalent among young men, with 1 in 25 reporting delivery only, 1 in 12 reporting receipt only, and 1 in 4 reporting both. Primary care physicians can consider assessing TFA among male patients and developing interventions to mitigate this behavior.VISUAL ABSTRACT.

Associations of food insecurity and material social support with parent and child mental health during COVID-19.

The rise of mental health problems during COVID-19 has been called a national crisis. Parents and caregivers reported parenting stress, anxiety, and depression, which may be exacerbated by economic insecurity. This study used longitudinal data to examine the association of food insecurity and material social support to subsequent parent and child mental health outcomes in the early weeks of COVID-19. Data were collected from a national convenience sample of U.S. parents (N = 359) at two time points: April 14, 2020 (T1) and April 30, 2020 (T2). Data were analyzed using multivariate linear and logistic regression analyses. Most of the sample were mothers (67.5%) and identified as White (69.6%). Among parents for whom services were applicable, over half (51.4%) were unable to receive free and reduced-cost school-based lunch. Food insecurity at T1 was significantly associated with higher odds of parental anxiety (OR = 1.52, p <.001) and depression (OR = 1.63, p <.001), as well as increased parenting stress (ß = 0.16, p =.008) and parental report of child anxiety (ß = 0.15, p =.014). Conversely, material social support was significantly associated with lower odds of parental anxiety (OR = 0.90, p =.014) and depression (OR = 0.85, p <.001), as well as lower levels of parenting stress (ß = -0.20, p =.001) and parental report of child anxiety (ß = -0.13, p =.028). Results suggest that household food insecurity may place parents and children at greater risk for mental health problems during COVID-19. However, access to tangible resources that offer material or financial support may be protective for both parent and child mental health. Study results suggest that policy interventions are needed to support the economic wellbeing of families during COVID-19.

Adverse Childhood Experiences and Spanking Have Similar Associations with Early Behavior Problems.

OBJECTIVES: To examine whether adverse childhood experiences (ACEs) and physical punishment (ie, spanking) are unique risk factors for behavior problems in early childhood, and whether ACEs moderate the associations of spanking with child behavior problems. STUDY DESIGN: We conducted prospective, longitudinal analyses on 2380 families in the Fragile Families and Child Wellbeing Study. Mothers reported outcomes of externalizing and internalizing behavior problems at age 5 years; and the main predictors, ACEs and spanking, at age 3 years. ACEs included 9 items: physical abuse, emotional abuse, physical neglect, emotional neglect, mother's exposure to intimate partner violence, parental mental health problem, parental substance use, parental incarceration, and parental death. Multilevel models examined the associations between ACEs, spanking, and behavior problems, and the moderating effect of ACEs in the associations of spanking with behavior problems. Analyses were adjusted for preexisting behavior problems, demographics, and neighborhood conditions. RESULTS: ACEs (ß = 0.028; P < .001) and spanking (ß = 0.041; P < .001) at 3 years were unique risk factors for increased externalizing behavior problems at 5 years, after controlling for covariates. The magnitude of the associations of ACEs and spanking with externalizing behavior were statistically indistinguishable. ACEs did not moderate the association between spanking and externalizing behavior. CONCLUSIONS: ACEs and spanking have similar associations in predicting child externalizing behavior. Results support calls to consider physical punishment as a form of ACE. Our findings also underscore the importance of assessing exposure to ACEs and physical punishment among young children and providing appropriate intervention to children at risk.

Parenting activities and the transition to home-based education during the COVID-19 pandemic.

This study reports on parent-child dynamics during initial COVID-19 related school closures, based on cross-sectional analyses of a survey that utilized a convenience sampling approach. Data were collected in April 2020, approximately five weeks after the World Health Organization declared that the Coronavirus was a pandemic. Participants (N = 405) were adults recruited throughout the U.S. This study examines data from parents (69% mothers and 31% fathers) with at least one child 0-12 years of age. The majority were White (71%) and 41% had at least a bachelor's degree. The majority of parents (78%) were educating their child at home due to COVID-19. Most (77.1%) reported use of online tools for at-home education, including educational apps, social media, and school-provided electronic resources. More than one-third (34.7%) of parents said their child's behavior had changed since the pandemic, including being sad, depressed, and lonely. Most parents were spending more time involved in daily caregiving of their children since COVID-19. Two out of every five parents met the PHQ-8 criteria for major depression or severe major depression (40.0%) and the GAD-7 criteria for moderate or severe anxiety (39.9%). Multivariate analyses indicated that, compared to non-depressed parents, parents who met criteria for probable major or severe depression (B = -0.16, 95% CI = [-0.29, -0.02], p = .021) and parenting stress (B = -0.37, 95% CI = [-0.47, -0.27], p < .001) were negatively associated with parents' perceived preparation to educate at home. Compared to parents with minimal or mild anxiety, parents with moderate or severe anxiety reported higher child anxiety scores (B = 0.17, 95% CI = [0.06, 0.28], p = .002). Parenting stress was also positively associated with higher child anxiety scores (B = 0.40, 95% CI = [0.32, 0.48], p < .001). Content analyses of open-ended questions indicated that school closures were a significant disruption, followed by lack of physical activity, and social isolation. Overall, study results suggested that parents' mental health may be an important factor linked to at-home education and child wellbeing during the pandemic.

Prevalence of Intimate Partner Violence and Beliefs About Partner Violence Screening Among Young Men.

PURPOSE: Few clinical guidelines focus on how physicians can identify intimate partner violence (IPV) perpetration or victimization among male patients, and little is known of men's experiences and beliefs regarding screening in health care settings. Our objective was to determine prevalence of men's experiences with IPV screening in health care settings and associations with men's beliefs regarding health care clinician identification of IPV. METHODS: Using a cross-sectional online survey of a nationally representative sample of 916 men aged 18-35 years, we conducted survey-weighted descriptive analyses to determine IPV prevalence, screening experiences and beliefs, and multivariate logistic regression to examine associations of demographics, IPV perpetration, and IPV victimization with men's screening experiences and beliefs. RESULTS: Of 916 men surveyed, 19% reported perpetration and 27% reported victimization in relationship with current or previous spouse/partner, 90% believed health care clinicians should ask about perpetration, 92% believed health care clinicians should ask about victimization, but only 11% had been asked about perpetration and 13% about victimization. Beliefs regarding IPV were associated with African American non-Hispanic race, IPV perpetration, and IPV victimization. Experiences being asked about IPV were associated with educational attainment and IPV perpetration. CONCLUSIONS: Among young US men, 9 in 10 support IPV identification by health care clinicians, nearly 1 in 5 report using IPV, but only about 1 in 10 report health care clinicians asking about IPV. These represent missed opportunities for health care IPV identification. Beliefs and experiences regarding health care IPV identification vary by race, education, and men's IPV perpetration and victimization. These disparities can inform tailored health care identification approaches.

Does community violence exposure moderate the associations between maternal spanking and early child behavior problems?

A robust research literature links parental spanking with negative behavioral outcomes for children, however, it remains unclear whether conditions in the community may moderate the associations between spanking and behavior problems in early childhood. In the current study, we examined whether community violence exposure moderated the associations of maternal spanking with externalizing and internalizing behavior problems of young children. The sample used in this study was urban families and their children ages 3-5 (n = 2,472). We used fixed effects regression models, which yield stronger statistical control for baseline behavior problems, selection bias, and omitted variables bias. Mother's spanking was associated with elevated levels of both externalizing (ß = .037, p < .001) and internalizing (ß = .016, p < .001) behavior problems. Community violence exposure also predicted higher levels of externalizing (ß = .071, p < .01) and internalizing (ß = .043, p < .05) behavior problems. Community violence exposure did not moderate the associations between maternal spanking and behavior problems. Professionals working with families should promote the use of nonphysical disciplinary practices, regardless of the level of violence and crime in the community in which the family resides.

Prescription Opioid Misuse and Intimate Partner Violence Perpetration among a Nationally Representative Sample of Young Men.

BACKGROUND: Drug use is associated with intimate partner violence (IPV) perpetration among men, but few studies have examined the relation between prescription opioid misuse and IPV perpetration. Objectives: The purpose of this study is to examine the relation between prescription opioid misuse and IPV perpetration while controlling for demographic, depression, alcohol, and illicit drug use risk factors among a non-clinical, nationally representative sample of young men aged 18-35. Methods: Cross-sectional survey in August 2014 of 1,053 partnered men aged 18-35 in a nationally representative sample of the adult U.S. population. The survey assessed physical IPV perpetration, depressive symptoms, alcohol misuse, marijuana use, illegal drug use, prescription opioid misuse, and demographic characteristics. We calculated descriptive statistics and conducted weighted bivariate and multivariate logistic regression to assess associations of IPV perpetration with prescription opioid misuse and other known IPV risk factors. Results: Weighted analyses show 19.4% of men reported IPV perpetration in the current or most recent relationship, and 7.3% reported prescription opioid misuse in the past year. After controlling for marijuana use, illegal drug use, depressive symptoms, and demographic characteristics, prescription opioid misuse in the past year (A.O.R. = 1.94, 95% CI = 1.33-2.84) was associated with increased odds of young men's physical IPV perpetration in the current or most recent relationship. Conclusions/importance: Prescription opioid misuse is associated with IPV perpetration at a population-level among young men and is not unique to clinical samples. Prevention and intervention strategies should be developed to simultaneously target prescription opioid misuse and IPV perpetration.

Mothers' and Fathers' Parenting Stress, Responsiveness, and Child Wellbeing Among Low-Income Families.

Robust research shows that parenting stress is associated with reduced parental sensitivity toward their children (i.e., parental responsiveness), thus negatively influencing child outcomes. While there is strong research supporting these associations, most studies utilize self-report measures of responsiveness and exclude fathers. This study examines whether observed parental responsiveness mediates the relationship between parenting stress and child cognitive development, prosocial behavior, and behavior problems in a large sample of diverse low-income families. Data were obtained from the Building Strong Families Project (N=1,173). Dyadic bootstrapped mediation models were estimated in Mplus. For mothers and fathers, parenting stress was negatively associated with responsiveness (B = -.08, 95% CI = [-.14, -.02], p = .012), and responsiveness was positively associated with child cognitive development (B = .15, 95% CI = [.11, .19], p < .001) and child prosocial behavior (B = .12, 95% CI = [.08, .15], p < .001). Mothers' responsiveness was negatively associated with child behavior problems (B = -.07, 95% CI = [-.13, -.01], p = .020), but fathers' responsiveness was not (B = -.01, 95% CI = [-.06, .05], p = .814). For mothers and fathers, parenting stress was indirectly related to child cognitive development and prosocial behavior via responsiveness. Indirect effects were not found for mothers or fathers when predicting child behavior problems. To improve children's wellbeing, interventions may consider strengthening responsiveness and reducing parental stress among both mothers and fathers.

Exploring the optimal allostatic load scoring method in women of reproductive age.

AIMS: The aim of this study was to determine the optimal allostatic load scoring method. DESIGN: This is a secondary analysis of data on women of reproductive age from the 2001-2006 National Health and Nutrition Examination Survey. METHODS: We created allostatic load summary scores using five scoring methods including the count-based, Z-Score, logistic regression, factor analysis and grade of membership methods. Then, we examined the predictive performance of each allostatic load summary measure in relation to three outcomes: general health status, diabetes and hypertension. RESULTS: We found that the allostatic load summary measure by the logistic regression method had the highest predictive validity with respect to the three outcomes. The logistic regression method performed significantly better than the count-based and grade of membership methods for predicting diabetes as well as performed significantly better for predicting hypertension than all of the other methods. But the five scoring methods performed similarly for predicting poor health status. CONCLUSION: We recommended the logistic regression method when the outcome information is available, otherwise the frequently used simpler count-based method may be a good alternative. IMPACT: The study compared different scoring methods and made recommendations for the optimal scoring approach. We found that allostatic load summary measure by the logistic regression method had the strongest predictive validity with respect to general health status, diabetes and hypertension. The study may provide empirical evidence for future research to use the recommended scoring approach to score allostatic load. The allostatic load index may serve as an 'early warning' indicator for health risk.

Father Early Engagement Behaviors and Infant Low Birth Weight.

Objective To examine the association of father early engagement behaviors and infant low birth weight (LBW) among unmarried, urban couples. Methods Participants were from the Fragile Families and Child Wellbeing Study, a birth-cohort study of urban families. We conducted cross-sectional analyses of data from interviews with unmarried mothers and fathers (N = 2726) that took place at the time of their child's birth. Early engagement behaviors were based on fathers' self-report of whether during the pregnancy they gave mothers money to buy things for the baby, helped in other ways like providing transportation to prenatal clinics, and attended the birth. Results Most (68.9%) fathers engaged in all three early engagement behaviors; 22% engaged in 2 behaviors; and 9.1% engaged in 1 or 0 early engagement behaviors. LBW more than doubled when comparing infants of fathers who engaged in all three early engagement behaviors (9.6% predicted probability of LBW) to those fathers who engaged in no early engagement behaviors (over 22% predicted probability of LBW). Conclusion Infant and maternal health may benefit from intervention to encourage positive father engagement during pregnancy.

The Association of Fathers' Parental Warmth and Parenting Stress to Child Behavior Problems.

This study examines whether fathers' parental warmth and parenting stress were associated with behavior problems when children were approximately 36-months of age, beyond the influence of maternal behaviors. Study participants were 3,342 low-income fathers and mothers who participated in the Building Strong Families (BSF) study. Cross-sectional regression analyses indicated that for unmarried nonresidential families, fathers' parental warmth and parenting stress were associated with child internalizing behavior problems; and fathers' parenting stress only was marginally associated with child externalizing behavior problems. For consistently cohabiting residential fathers, only fathers' parenting stress was marginally associated with child internalizing behavior problems. No associations of fathers' parental warmth and parenting stress on either internalizing or externalizing behavior problems were observed in married families. Overall, study results suggest that fathers' parental warmth and parenting stress may have a modest positive association on the development of child internalizing behavior problems particularly in vulnerable families (e.g., families in which fathers were nonresidential).

Predictors of Parental Use of Corporal Punishment in Ukraine.

Despite a great deal of evidence that corporal punishment is harmful, corporal punishment is still very prevalent worldwide. We examine predictors of different types of corporal punishment among Ukrainian mothers in 12 communities across Ukraine. Findings suggest that maternal spirituality, maternal coping styles, family communication, and some demographic characteristics are predictive of mothers' use of corporal punishment.

Wait until your father gets home? Mother's and fathers' spanking and development of child aggression.

This study examined whether fathers' and mothers' spanking contributed to development of child aggression in the first 5 years of life. We selected parents (N =1,298) who were married or cohabiting across all waves of data collection. Cross-lagged path models examined fathers', mothers', and both parents' within-time and longitudinal associations between spanking and child aggression when the child was 1, 3, and 5 years of age. Results indicated that mothers spanked more than fathers. When examining fathers only, fathers' spanking was not associated with subsequent child aggression. When examining both parents concurrently, only mothers' spanking was predictive of subsequent child aggression. We found no evidence of multiplicative effects when testing interactions examining whether frequent spanking by either fathers or mothers was predictive of increases in children's aggression. This study suggests that the processes linking spanking to child aggression differ for mothers and fathers.

Identification of genes expressed by immune cells of the colon that are regulated by colorectal cancer-associated variants.

A locus on human chromosome 11q23 tagged by marker rs3802842 was associated with colorectal cancer (CRC) in a genome-wide association study; this finding has been replicated in case-control studies worldwide. In order to identify biologic factors at this locus that are related to the etiopathology of CRC, we used microarray-based target selection methods, coupled to next-generation sequencing, to study 103 kb at the 11q23 locus. We genotyped 369 putative variants from 1,030 patients with CRC (cases) and 1,061 individuals without CRC (controls) from the Ontario Familial Colorectal Cancer Registry. Two previously uncharacterized genes, COLCA1 and COLCA2, were found to be co-regulated genes that are transcribed from opposite strands. Expression levels of COLCA1 and COLCA2 transcripts correlate with rs3802842 genotypes. In colon tissues, COLCA1 co-localizes with crystalloid granules of eosinophils and granular organelles of mast cells, neutrophils, macrophages, dendritic cells and differentiated myeloid-derived cell lines. COLCA2 is present in the cytoplasm of normal epithelial, immune and other cell lineages, as well as tumor cells. Tissue microarray analysis demonstrates the association of rs3802842 with lymphocyte density in the lamina propria (p = 0.014) and levels of COLCA1 in the lamina propria (p = 0.00016) and COLCA2 (tumor cells, p = 0.0041 and lamina propria, p = 6 × 10(-5)). In conclusion, genetic, expression and immunohistochemical data implicate COLCA1 and COLCA2 in the pathogenesis of colon cancer. Histologic analyses indicate the involvement of immune pathways.

Implementing Next-Generation Sequencing Process Changes to Increase Capacity and Improve Timeliness of Molecular Biomarker Profiling for Lung Cancer Patients.

BACKGROUND: Faced with expansion of molecular tumor biomarker profiling, the molecular genetics laboratory at Kingston Health Science Centre experienced significant pressures to maintain the provincially mandated 2-week turnaround time (TAT) for lung cancer (LC) patients. We used quality improvement methodology to identify opportunities for improved efficiencies and report the impact of the initiative. METHODS: We set a target of reducing average TAT from accessioning to clinical molecular lab report for LC patients. Process measures included percentage of cases reaching TAT within target and number of cases. We developed a value stream map and used lean methodology to identify baseline inefficiencies. Plan-Do-Study-Act cycles were implemented to streamline, standardize, and automate laboratory workflows. Statistical process control (SPC) charts assessed for significance by special cause variation. RESULTS: A total of 257 LC cases were included (39 baseline January-May 2021; 218 post-expansion of testing June 2021). The average time for baseline TAT was 12.8 days, peaking at 23.4 days after expansion of testing, and improved to 13.9 days following improvement interventions, demonstrating statistical significance by special cause variation (nonrandom variation) on SPC charts. CONCLUSIONS: The implementation of standardized manual and automated laboratory processes improved timeliness of biomarker reporting despite the increasing volume of testing at our center.

Parental Spanking and Subsequent Risk for Child Aggression in Father-Involved Families of Young Children.

This study examined separate and combined maternal and paternal use of spanking with children at age 3 and children's subsequent aggressive behavior at age 5. The sample was derived from a birth cohort study and included families (n = 923) in which both parents lived with the child at age 3. In this sample, 44% of 3-year-olds were spanked 2 times or more in the past month by either parent or both parents. In separate analyses, being spanked more than twice in the prior month at age 3, by either mother or father, was associated with increased child aggression at 5 years. In combined analyses, there was a dose-response association; the greatest risk for child aggression was reported when both parents spanked more than twice in the prior month (adjusted odds ratio: 2.01; [confidence interval: 1.03-3.94]). Violence prevention initiatives should target and engage mothers and fathers in anticipatory guidance efforts aimed at increasing the use of effective and non-aggressive child discipline techniques and reducing the use of spanking.