Family social capital mediates the effect of poverty on children's anxiety and depression.

This study aims to examine how family social capital mediates the relationship between poverty and children's anxiety and depression in mainland China. Data were collected with multistage cluster random sampling method in Xiushui City in mainland China. The sample comprised 1,314 school-aged children (Grades 4-9). Data included children's basic demographics, internalizing symptoms, and parenting process. Structural equation modeling was used to test the hypothesized model. The results demonstrated a good fit for the sample. The overall model explained 12.7% and 16.1% of the variance for children's anxiety and depression, respectively. Poverty had a significantly direct effect on children's anxiety and depression, and family social capital could partially mediate the influence of poverty on children's internalizing symptoms. Family social capital plays an important mediating role between poverty and children's mental health. Our findings provide strong evidence for social work intervention. Family social capital is essential for parent-child interactions, especially in underprivileged families.

Deciphering the biotic and abiotic drivers of coalescence asymmetry between soil and manure microbiomes.

Manure application improves soil fertility, yet its implications on the success of invasion of manure-borne microorganisms in the soil are poorly understood. Here, we assessed the importance of abiotic and biotic factors in modulating the extent to which manure-borne fungal and bacterial communities can invade resident soil microbial communities. For this purpose, we applied varying frequencies of two different amounts of manure to nine soils differing in physico-chemical properties, as well as in land-use history, over 180 days and monitored changes in bacterial and fungal communities. Variance partitioning revealed differential contributions of abiotic and biotic factors to invasion success, which together accounted for up to 82 % of the variance explained. We showed that the effects of interactions between biotic and abiotic factors increased with coalescence frequency and manure amount for the bacterial and fungal communities, respectively. Both abiotic and biotic factors were important for modulating coalescence asymmetry for the bacterial community, whereas abiotic factors had a greater effect on the fungal community. These results provide new insights into the drivers of coalescence events between manure and resident soil microbial communities. Moreover, our findings highlight the roles of the mixing ratio and frequency of coalescence events in modulating the survival of manure-borne microorganisms.

Global-scale no-tillage impacts on soil aggregates and associated carbon and nitrogen concentrations in croplands: A meta-analysis.

No-tillage treatment, including no-tillage with straw retention (NTS) and without (NT), has been widely used as an efficient and sustainable alternative to conventional tillage with straw retention (CTS) and without (CT) and greatly affects soil physical quality and organic matter dynamics in cropland ecosystems. Although some studies have reported the effects of NTS on soil aggregate stability and soil organic carbon (SOC) concentration, the underlying mechanisms of how soil aggregates, aggregate-associated SOC and total nitrogen (TN) respond to no-tillage remain unclear. Through a global meta-analysis of 91 studies in cropland ecosystems, we evaluated the effects of no-tillage on soil aggregates and their associated SOC and TN concentrations. On average, no-tillage treatment significantly decreased the proportions of microaggregates (MA) by 21.4 % (95 % CI, -25.5 to -17.3 %) and silt+clay size particles (SIC) by 24.1 (95 % CI, -30.9 to -17.0 %), and increased the proportions of large macroaggregate (LA) by 49.5 % (95 % CI, 36.7-63.0 %) and small macroaggregate (SA) by 6.1 % (95 % CI, 2.0-10.9 %) compared to those in conventional tillage. The SOC concentrations for all three aggregate sizes increased significantly with no tillage: for LA by 28.2 % (95 % CI, 18.8-39.5 %), SA by 18.0 % (95 % CI, 12.8-23.3 %), and MA by 9.1 % (95 % CI, 2.6-16.8 %). TN also increased significantly for all sizes with no tillage, with LA by 13.6 % (95 % CI, 8.6-17.6 %), SA by 11.0 % (95 % CI, 5.0-17.0 %), MA by 11.7 % (95 % CI, 7.0-16.4 %), and SIC by 7.6 % (95 % CI, 2.4-13.8 %). The magnitude of the no-tillage treatment effect on soil aggregation, aggregate-associated SOC and TN varied with the environmental and experimental conditions. The positive effect on the proportions of LA occurred with initial soil organic matter (SOM) content >10 g kg-1, whereas SOM <10 g kg-1 did not change significantly. Additionally, the effect size of NTS compared with CTS was lower than that of NT compared with CT. These findings suggest that NTS may promote physically protective SOC accumulation through the formation of macroaggregates by reducing disturbance destruction and increasing plant-derived binding agents. The findings highlight that no-tillage may enhance the formation of soil aggregates and the associated SOC and TN concentrations in global cropland ecosystems.

Family Economic Strain and Adolescent Aggression during the COVID-19 Pandemic: Roles of Interparental Conflict and Parent-Child Conflict.

Although the link from family economic strain to adolescent aggression has frequently been hypothesized, the results are mixed. Both interparental conflict and parent-child conflict are considered to be potential mediators of this link. However, the empirical evidence supporting this proposition is lacking. The present study investigated the direct effect of family economic strain on adolescent aggression as well as indirect effects through interparental conflict and parent-child conflict. Based on multi-informant data from 971 families with a child in middle and high schools in Y City, in Shanxi Province, structural equation modeling is conducted to examine the proposed theoretical model. Findings show that family economic strain has no significant direct impact on adolescent aggression. Interparental conflict and parent-child conflict mediate the link between family economic strain and adolescent aggression simultaneously and sequentially. This study expands current literature and deepens our understanding of the mechanisms underlying the relationship between family economic strain and adolescent aggression. Implications for policies and interventions to reduce the risk of adolescent aggression are discussed.

Mixed-Mode Bacterial Transmission via Eggshells in an Oviparous Reptile Without Parental Care.

Symbiotic microorganisms play important roles in maintaining health and facilitating the adaptation of the host. We know little about the origin and transgenerational transmission of symbiotic bacteria, especially in egg-laying species without parental care. Here, we investigated the transmission of bacterial symbionts in the Chinese three-keeled pond turtle (Mauremys reevesii), a species with no post-oviposition parental care, by evaluating contributions from potential maternal and environmental sources to eggshell bacterial communities. Using 16S rRNA amplicon sequencing, we established that the bacterial communities of eggshells were similar to those of the maternal cloaca, maternal skin, and nest soil, but distinct from those of surface soil around nest and pond water. Phylogenetic structure analysis and source-tracking models revealed the deterministic assembly process of eggshell microbiota and high contributions of the maternal cloaca, maternal skin, and nest soil microbiota to eggshell bacterial communities. Moreover, maternal cloaca showed divergent contribution to eggshell microbiota compared with two other main sources in phylogenesis and taxonomic composition. The results demonstrate a mixture of horizontal and vertical transmission of symbiotic bacteria across generations in an oviparous turtle without parental care and provide insight into the significance of the eggshell microbiome in embryo development.

Perceived discrimination, integration, depression and behavioral problems among Chinese migrant children: Examining the moderating role of migration pattern in the rural-urban dual system.

INTRODUCTION: The purpose of this study is to investigate the influence of perceived discrimination on children's depression and behavioral problems via the mediator of integration among Chinese migrant children. Rural-urban differences in the proposed relationships are also examined. METHODS: The sample included 484 migrant children (Mean age = 11.65 years; 52.9% girls), which was collected through multi-stage cluster random sampling in Kunming, Southwest China. Structural equation modelling (SEM) was adopted for data analysis. RESULTS: Results indicate that perceived discrimination reduces the integration of Chinese migrant children, which in turn, leads to their higher levels of depression and more behavioral problems. The multi-group analysis on rural-urban differences reveals that the effects of discrimination on depression and behavioral problems are significant among rural-urban migrants but not among urban-urban ones. CONCLUSIONS: This study contributes to current knowledge by revealing the mechanisms among perceived discrimination, integration, depression and behavioral problems of Chinese migrant children. The migration pattern differences in terms of their depression and behavioral problems are also highlighted.

The potential role of fertilizer-derived exogenous bacteria on soil bacterial community assemblage and network formation.

Manure fertilization contributes to crop production and sustainable agriculture by introducing large amounts of nutrients and exogenous microbes into soil. However, the contribution of exogenous microbes in shaping soil bacterial community and network structure after fertilization are still controversial. In this study, bacterial communities and network structure that received unsterilized (R + C) or sterilized (R + SC) manure fertilizers, as well as no fertilizer control (R), were characterized using high throughput sequencing. Results showed that the relative abundance of fertilizer-derived OTUs decreased from 10.4% to 4.6% after 90 days incubation, while the Bray-Curtis distance between the control and fertilization group (R + C and R + SC) gradually increased with the culture time. It can be supposed that manure fertilization altered soil bacterial communities by interfering the growth of indigenous bacteria rather than the colonization of fertilizer-derived bacteria. Network analysis showed that a subset of the fertilizer-derived OTUs identified as Xanthomonadales order and Promicromonospora, Constrictibacter genera acted as connectors between modules. They enhanced the interactions not only between soil-derived OTUs and fertilizer-derived OTUs, but also within indigenous bacteria, supported that the introduction of fertilizer-derived exogenous bacteria contributes large to soil bacterial network association. Moreover, fertilizer-derived OTUs presented to be positively correlated with soil pH, while majority soil-derived OTUs presented to be negatively correlated with various physicochemical variables (pH, DOC, NO3-, and LAP). Our study highlighted the critical role of fertilizer-derived bacteria in regulating indigenous soil microbial community and network formation after fertilization.

Copiotrophic taxa in pig manure mitigate nitrogen limitation of soil microbial communities.

Microbial nitrogen (N) limitation is a common problem in terrestrial ecosystems. Pig manure, a type of solid waste, is increasingly applied to improve soil N availability in agriculture through inputs of organic matter and inorganic N. Pig manure application also introduces a lot of exogenous microorganisms, which have distinctly different N requirements and metabolic properties, into the resident soil microbial community. However, the impacts of these manure-borne microorganisms on soil N cycling have not been well determined. Here, we investigated effects of manure-borne microorganisms on the N limitation of soil microorganisms using an ecoenzymatic stoichiometry analysis. We monitored microbial communities over a 90-day period in a laboratory-controlled experiment with four treatments: (1) non-sterilized soil mixed with non-sterilized manure (S-M), (2) non-sterilized soil mixed with sterilized manure (S-sM), (3) sterilized soil mixed with non-sterilized manure (sS-M), and (4) non-sterilized soil without manure addition (S, the control). The microbial N limitations were significantly mitigated in both S-M and sS-M. By contrast, the S-sM and S showed high levels of microbial N limitation, likely stemming from differences in the microbial functional composition. We found chitin-degrading bacteria were the dominant copiotrophic manure-borne bacteria associated with N mineralization, and they may improve soil N availability. We further identified several copiotrophic manure-borne bacteria in S-M and sS-M, and their abundances had significantly negative correlation with the level of N limitation and significantly positive correlation with the stoichiometric homeostasis. As these copiotrophic taxa can maintain homeostasis through regulating enzymatic activities, our results indicate that copiotrophic taxa in pig manure contribute to the mitigation of soil microbial N limitation. Our study also highlights the invasiveness capacity of manure-borne microorganisms in soil and evaluates the biotic effects of manure application on soil N cycling.

Exploring the impact of marital relationship on the mental health of children: Does parent-child relationship matter?

This study aimed to examine the associations between marital relationships and parent-child relationships on children's mental health. Participants included 19,487 students from the 2013-2014 baseline China Education Panel Survey. Structural equation modeling was applied to analyze the data and results revealed that marital and parent-child relationships positively affected children's mental health. Parent-child relationship also played a mediating role between marital relationship and children's mental health. The unique contributions of this study and its theoretical and practical implications were discussed.

Effects of multidimensional child poverty on children's mental health in Mainland China.

This study aimed to investigate the effects of multidimensional child poverty on children's mental health in Mainland China. The data were based on a survey of children in 2015 conducted by multi-stage cluster random sampling method in Xiushui, where levels of poverty are very high. Structural equation model was adopted to analyze the data and test the hypothesized model. Results showed that multidimensional child poverty had a significant effect on both negative mental health and positive mental health of children, and the seven indicators of multidimensional child poverty all had slightly different effects on children's mental health.

The mediating effect of neighbor relationship between community social support and elderly's subjective well-being in China.

This study aimed to investigate the relationship between community social support, neighbor relationship, and the subjective well-being of the elderly in China. Structural equation modeling was used to test the hypothesized model based on a sample of 2732 senior adults from 2010 Chinese General Social Survey. Results showed that community social support could indirectly affect the subjective well-being of the elderly through the complete mediating effect of neighbor relationship, although there was no significant correlation between community social support and the elderly's subjective well-being. Implications for theory, social work, and social policy were discussed.

Effects of multi-dimensional social capital on mental health of children in poverty: An empirical study in Mainland China.

Using the data of 1314 underprivileged children in Xiushui, China, this study adopted an ecological framework to explore how social capital embedded in family, peer, school, and community interplay and affect the mental health of poor children. Structural equation modeling was employed to verify the hypothesized model. The results demonstrated that higher levels of family, peer, and school social capital were all associated with better mental health outcomes of children in poverty. Moreover, family, peer, and school social capital fully mediated the effect of community social capital on children's mental health. Theoretical and practical implications were discussed.

The mediating effect of self-esteem on the relationship of living environment, anxiety, and depression of underprivileged children: A path analysis in Chinese context.

This study aimed to explore the relationships of living environment, self-esteem, anxiety, and depression of children in poverty. A sample of 1314 children (grades 4-9) was collected by a school-based survey in Xiushui city, Mainland China. Structural equation modeling was adopted to analyze the data and verify the hypothesized model. Results showed that living environment was negatively correlated with poor children's anxiety and depression levels, and self-esteem had a mediating effect on the relationship between living environment and the mental health of underprivileged children. Based on these findings, we proposed relevant theoretical and practical implications.

Effect of Different Filler Wires on Mechanical Property and Conductivity of Aluminum-Copper Joints.

The 1060 aluminum and T2 copper were joined by the pulsed double electrode gas metal arc welding (DE-GMAW) brazing method by using four types of filler wires, namely pure aluminum (Al) ER1100, aluminum-magnesium (Al-Mg) ER5356, aluminum-silicon (Al-Si) ER4043, and Al-Si ER4047, respectively. The effects of different types of filler wires on intermetallic compounds, microhardness tensile strength, and conductivity of joints were investigated. The results showed that a lot of brittle intermetallic compounds laying in the copper side brazing interface zone were generated using pure Al, Al-Mg, and Al-Si filler wires, which caused the change of microhardness, tensile strength, and the conductivity of joints. Meanwhile, with the increase in Si elements contents for Al-Sifiller wires, the thickness of the intermetallic compound layers decreased obviously, which was only up to 3 µm and the conductivity of the joints decreased. In addition, the microhardness, tensile strength, and the conductivity of the joints, when using Al-Sifiller wires, was higher than that using pure Al and Al-Mg filler wires. Hence, in comparison to the pure filler wires and Al-Mg filler wires, the Al-Si filler wires were more suitable for Al-Cu joints by DE-GMAW as Si element content was lower.

Intergenerational relationship, family social support, and depression among Chinese elderly: A structural equation modeling analysis.

OBJECTIVE: This study aimed to investigate the association among intergenerational relationship, family social support, and elderly's depression symptoms. METHOD: Data were obtained from China Longitudinal Aging Social Survey (CLASS) 2014, which is a continuous large-scale national social survey project conducted by the National Survey Research Center at Renmin University of China. Stratified multi-stage probability sampling method was used to collect the sample (N = 11,511). Structural equation modeling was adopted to analyze the association among intergenerational relationship, family social support, and elderly's depression. RESULTS: Intergenerational relationship and family social support were negatively correlated with an elderly's depression level. Elderly who enjoyed positive intergenerational relationships with their children would experience adequate family social support and likely have few depression symptoms. CONCLUSION: Intergenerational relationship directly affected the elderly's depression level. Family social support played a mediating role between intergenerational relationship and depression. Our findings could contribute to current theories and knowledge and provide implications in social policy, pension service, and social work intervention for the elderly in China.

Environmental fungi and bacteria facilitate lecithin decomposition and the transformation of phosphorus to apatite.

Organophosphorus compounds (OP) are stable P source in nature, and can increase eutrophication risk in waterbodies. Lecithin was the most difficult OP to be broken down. In this study, two typical phosphate-solubilizing microorganisms, Aspergillus niger and Acinetobacter sp., were applied to evaluate their ability to decompose both inorganic phosphates and lecithin. A. niger and Acinetobacter sp. could solubilize calcium phosphates by secreting various organic acids, e.g., oxalic and formic acids. The fungus, A. niger, shows significantly higher ability of solubilizing these inorganic phosphates than Acinetobacter sp., primarily due to its secretion of abundant oxalic acid. However, the bacterium, Acinetobacter sp., could secrete more acid phosphatase than A. niger for lecithin decomposition, i.e., 9300 vs. 8500 µmol L-1 h-1. Moreover, after addition of CaCl2, the released P from lecithin was transformed to stable chlorapatite in the medium. To the contrast, Ca cations inclined to form calcium oxalate (rather than stable phosphate mineral) after the incubation of A. niger, as it induced relatively acidic environment after breaking down lecithin. Therefore, this work sheds light on the bright future of applying bacteria and Ca cations in OP pollutant management.

The Freedom to Pursue Happiness: Belief in Free Will Predicts Life Satisfaction and Positive Affect among Chinese Adolescents.

A small amount of research has examined the association between the belief in free will and subjective well-being (SWB) among Western laypersons from individualist cultures. However, no study has examined this association among participants from collectivist cultures (e.g., Eastern Asian cultures). Therefore, in this study, we explored this association among two large, independent cohorts of Chinese adolescents (N1 = 1,660; N2 = 639; high school students). The belief in free will was measured by a self-reported questionnaire (Cohorts 1 and 2) and a two-alternative forced choice question regarding the existence of free will (Cohort 2). SWB included cognitive well-being (life satisfaction) and affective well-being (positive and negative affect) in both cohorts. Data analyses indicated that a stronger belief in free will was consistently associated with higher life satisfaction and positive affect in both cohorts. Our investigation provides evidence supporting the cultural generality of the positive effects of believing in free will on SWB.

Inactivation of zebrafish mrf4 leads to myofibril misalignment and motor axon growth disorganization.

Mrf4 is a basic helix-loop-helix (bHLH) transcription factor associated with myogenesis. Two mrf4 transcripts, mrf4_tv1 and mrf4_tv2, were identified in zebrafish generated by alternative splicing. To study their biological functions, we separately injected the Mrf4-morpholinos, including MO1 (mrf4_tv1:mrf4_tv2 knockdown), MO2+MO3 (mrf4_tv1:mrf4_tv2 knockdown), MO3 (mrf4_tv1 knockdown), and MO4 (mrf4_tv2 knockdown), into zebrafish embryos to observe mrf4 gene knockdown phenotypes. No phenotypic abnormalities were observed following injection with 0.5 ng of MO1 but those injected with 4.5, 9, or 13.5 ng displayed curved-body phenotypes, such as indistinct somite boundaries, and a lack of uniformly sized cell blocks. Similar results were also observed in the (MO2+MO3)-, MO3-, and MO4-injected groups. To further investigate the molecular mechanisms that lead to curved-body phenotypes, we stained embryos with alpha-bungrotoxin and specific monoclonal antibodies F59, Znp1, and Zn5 to detect morphological changes in acetyl-choline receptor (AChR) clusters, muscle fibers, common path of the primary neurons, and secondary neurons axonal projections, respectively. Our results show that the muscle fibers of mrf4_(tv1:tv2)-morphant aligned disorderly and lost their integrity and attachment, while the defects became milder in either mrf4_tv1-morphant or mrf4_tv2-morphant. On the other hand, reduced axonal projections and AChR clusters were found in both mrf4_tv2-morphant and mrf4_(tv1:tv2)-morphant but distributed normally in the mrf4_tv1-morphant. We conclude that Mrf4_tv2 is involved in alignment of muscle fibers, and Mrf4_tv1 might have cooperative function with Mrf4_tv2 in muscle fiber alignment, without affecting the muscle-nerve connection.