ABSTRACT
Dissolved organic matter (DOM) is the largest carbon pool and directly affects the biogeochemistry in lakes. In the current study, fourier transform ion cyclotron mass spectrometry (FT-ICR-MS) combined with fluorescent spectroscopy was used to assess the molecular composition and driving mechanism of DOM in 22 plateau lakes in Mongolia Plateau Lakes Region (MLR), Qinghai Plateau Lakes Region (QLR) and Tibet Plateau Lakes Region (TLR) of China. The limnic dissolved organic carbon (DOC) content ranged from 3.93 to 280.8 mg L-1 and the values in MLR and TLR were significantly higher than that in QLR. The content of lignin was the highest in each lake and showed a gradually decreasing trend from MLR to TLR. Random forest model and structural equation model implied that altitude played an important role in lignin degradation while the contents of total nitrogen (TN) and chlorophyll a (Chl-a) have a great influence on the increase of DOM Shannon index. Our results also suggested that the inspissation of DOC and the promoted endogenous DOM production caused by the inspissation of nutrient resulted in a positive relationship between limnic DOC content and limnic factors such as salinity, alkalinity and nutrient concentration. From MLR to QLR and TLR, the molecular weight and the number of double bonds gradually decreased but the humification index (HIX) also decreased. In addition, from the MLR to the TLR, the proportion of lignin gradually decreased, while the proportion of lipid gradually increased. Both above results suggested that photodegradation was dominated in lakes of TLR, while microbial degradation was dominated in lakes of MLR.
Subject(s)
Dissolved Organic Matter , Lakes , Lakes/chemistry , Chlorophyll A , Cyclotrons , Fourier Analysis , Lignin , Mass Spectrometry , Spectrum Analysis , ChinaABSTRACT
Dispersal is an essential natural process that influences community assembly, yet directional dispersal through wind and water may have distinctive effects. Environmental and spatial factors jointly influence community structure, but their relative importance is anticipated to vary with spatial distance, dispersal mode, and season. Accordingly, a systemic survey was conducted in subtropical Chinese mountain lotic systems to distinguish the relative contributions of environmental control and spatial structuring upon communities of macroinvertebrates with different dispersal ability. Macroinvertebrate samples were collected from the upper reaches and five tributaries of the Hanjiang River in October 2017 (autumn) and April 2018 (spring). These macroinvertebrates were identified and classified into three dispersal groups: aquatic passive (AqPa), terrestrial passive (TePa), and terrestrial active (TeAc). Variation partitioning analyses were performed on environmental factors and different sets of spatial factors (overland dispersal: Overland, directional downwind dispersal: AEM_Wind, along watercourse dispersal: Watercourse, and directional downstream dispersal: AEM_Water). Findings showed that both environmental filtering and spatial structuring influenced the structure of macroinvertebrate metacommunities. For AqPa and TePa groups, pure environmental effects were stronger than pure spatial effects based on most distance matrices; however, in AEM_Water, the effects of spatial processes surpassed those of environmental filtering. For TeAc group, the role of environmental control and spatial structuring varied depending on different spatial models. The results also highlighted seasonal shifts in metacommunity structuring processes. Spatial structures featuring direction, especially AEM_Water, were predominant in explaining the construction of macroinvertebrate communities. This work suggests that directional dispersal should be explicitly considered when examining the structure of ecological communities.
Subject(s)
Ecosystem , Rivers , Biota , Triethylenephosphoramide , WaterABSTRACT
We measured the concentrations of Cr, Fe, Ni, Cu, Zn, Cd, Pb and Hg, and the stable isotope ratios (δ13C and δ15N) in 87 fish samples within 12 economic fish species collected from the Dongting Lake, the second largest freshwater lake in China. With few exceptions in concentration of Cr, most of fish species showed lower concentrations of the 8 metals than legislation thresholds. Piscivorous fishes had significantly higher values of δ15N (possessing higher trophic level) and metal concentrations than planktivorous and herbivorous fishes. Moreover, demersal fishes showed higher concentrations of Cu, Zn, Cd and Pb than pelagic and benthopelagic fishes. We found positive correlations between concentrations of Fe, Pb and Hg and δ15N ratio, confirming the biomagnification of the three metals through trophic transfer. In contrast, fishes showed clear growth dilution effect for Ni, Zn, Cu, Pb and Hg, indicated by the negative correlations between their concentrations and fish weight or length. Multiple regression analysis demonstrated that growth dilution and biomagnification effects simultaneously governed the metal concentrations in fish muscle, and the two effects' importance varied among different metals. The human health risk assessment indicated that all 8 metals gave target hazard quotient (THQ) values < 1.0, i.e., the estimated daily intake (EDI) of metals did not exceed the oral reference dose (RfD), indicating a safe consumption of these fish species for consumer. Our study provides comprehensive approaches to better understand the determining processes and potential risk of heavy metals in freshwater lake fishes.
Subject(s)
Metals, Heavy , Water Pollutants, Chemical , Animals , Body Size , China , Ecosystem , Environmental Monitoring , Fishes , Habits , Humans , Lakes , Metals, Heavy/analysis , Risk Assessment , Water Pollutants, Chemical/analysisABSTRACT
Trace metal contamination in water and bioaccumulation in aquatic organisms are human health risks of increasing concern. However, the bioaccumulation of trace metals in the organs of the mussel Cristaria plicata in Dongting Lake, China and the human health risks of mussel consumption are largely unknown. We investigated the concentrations of 15 trace metals and metalloids in surface water, sediments, and C. plicata organs (foot, gill, mantle, and visceral mass) and quantified the bioaccumulation and human health risk of these trace metals in specimens collected from Dongting Lake. The concentrations of most metals in surface water exceeded previously published background values. In contrast, the concentrations of most metals in sediments showed a decreasing trend. Overall, the metal concentrations in the gill and visceral masses of C. plicata were higher than those in the foot and mantle, and higher bioaccumulation capacities were observed for essential metals than for nonessential metals. The mean concentrations of the trace elements Zn, Pb, Cd, As, Cu, and Cr in C. plicata foot samples were lower than the threshold values established by international and Chinese organizations. The estimated daily intake (EDI) values of the essential metal Mn in C. plicata foot was higher than the recommended tolerable daily intake (TDI) values for juveniles. Only Mn for juveniles and As for both juveniles and adults may pose noncarcinogenic health risks through foot consumption. The hazard index (HI) values for adults and juveniles were higher than 1, suggesting significant risks of noncarcinogenic effects to humans by exposure to multiple metals.
Subject(s)
Bivalvia , Metals, Heavy , Water Pollutants, Chemical , Adult , Animals , Bioaccumulation , China , Environmental Monitoring , Geologic Sediments , Humans , Lakes , Metals, Heavy/analysis , Risk Assessment , Water Pollutants, Chemical/analysisABSTRACT
The Qinghai-Tibetan Plateau has a booming tourism industry and an increasingly sophisticated road system. There is a paucity of studies quantifying the contributions of anthropogenic and natural factors to microplastic pollution in remote plateau areas. In this study, water and sediment samples were collected from eight lake tourist attractions and four remote lakes in northern and southern regions of the Qinghai-Tibetan Plateau. Microplastics were detected in all samples, with a mean abundance of 0.78 items/L in water and 44.98 items/kg in sediment. The abundance of microplastics in the study area was lower than previously observed in more populated areas of China. Small-sized (<1 mm and 1-2 mm), fiber, and transparent microplastics were predominant, with polyethylene and polypropylene microplastics as the primary polymer types. The compositions of microplastic communities indicated that tourism and road networks were the major sources of microplastics in the lakes. Distance-decay models revealed greater influence of environmental distances on microplastic community similarity than geographic distance. Compared to climate factors, urban spatial impact intensity and traffic flow impact played a leading role in the structuring of microplastic communities in lake water and sediment. Our findings provide novel quantitative insights into the role of various factors in shaping the distribution patterns of microplastic communities in plateau lakes.
ABSTRACT
The impact of watershed comprehensive management (WCM) on per- and polyfluoroalkyl substances (PFAS) pollution in rapidly urbanizing areas remains unclear. In a typical rapidly urbanizing watershed of Shenzhen, China, we investigated temporal variations in contamination level, primary source and ecological risk of 50 emerging and legacy PFAS, as well as the replacement trends of emerging PFAS before and after WCM during a six-year sampling campaign. We found that large-scale dredging was a non-negligible factor in abnormally increased PFAS concentrations (6.43 %-456.16 %) during WCM through their release from river sediments. To better characterize the diverse and complex PFAS contamination, a novel pollution assessment method, PFAS "diversity", was adopted based on a modified Shannon-Weiner diversity index and Pielou evenness index, reflecting numbers of PFAS detected and how evenly each PFAS contributed to the total PFAS concentrations at specific sampling sites. Importantly, we found that the Pielou evenness index can indicate and quantify abnormal pollution sources (especially point sources) along the river. The results revealed that WCM did not effectively reduce total PFAS concentrations and diversity in the rapidly urbanizing watershed but obviously improved point source pollution. Furthermore, 6:2 polyfluorinated phosphate diesters and hexafluoropropylene oxide dimer acid (GenX) that posed high ecological risks emerged and the number of sampling sites with high risk increased from 16 to 20 after WCM. Finally, we summarize several important issues related to PFAS contamination during WCM and propose specific countermeasures, such as adopting environmental dredging and reducing the proportion of ecological water replenished by wastewater treatment plant effluent for better control of PFAS pollution. Our study highlighted the limited effectiveness of WCM in mitigating PFAS pollution and the importance of emerging contaminant regulation in rapidly urbanizing watersheds during WCM.
Subject(s)
Environmental Monitoring , Fluorocarbons , Rivers , Water Pollutants, Chemical , Water Pollutants, Chemical/analysis , Fluorocarbons/analysis , Rivers/chemistry , China , UrbanizationABSTRACT
Plateau lakes characterized by salinization and eutrophication are essential aquatic ecosystems. A myriad of microorganisms serve as crucial biological resources in plateau lakes and drive the elemental cycles of these ecosystems. Currently, there is a paucity of knowledge regarding the impacts of salinization and eutrophication dynamics on the microbiota in plateau lakes. Here, high-throughput sequencing of the 16S ribosomal RNA genes (V4 region) was used to characterize microbial community structure and assembly in plateau lakes with different salinities and trophic levels. Water samples were collected at 191 sites across 24 lakes on the Qinghai-Tibet and Inner Mongolia Plateaus in northern China. The results showed that high salinity considerably reduced microbial alpha-diversity and niche breadth while increasing within-group similarity among various lake types. High salinity additionally decreased the complexity of microbial networks and enhanced network robustness. The assembly of microbial communities was primarily governed by deterministic processes in high-salinity and eutrophic low-salinity lakes. At decreased salinity, trophic level played a leading role in shaping microbial community structure, and the ecological processes shifted from deterministic processes driven by high salinity to eutrophication-driven deterministic processes. The biomarkers also varied from taxa adapted to high-salinity environments (e.g., Nanoarchaeaeota, Rhodothermia) to those suited for living in freshwater and low-salinity habitats (e.g., Alphaproteobacteria, Actinobacteria). In the case of eutrophication, Actinobacteria, Chloroflexi, and Cyanobacteria became the dominant taxa. Our findings indicate that decreased salinity enables trophic level to play an enhanced role in shaping microbial community structure and assembly in plateau lakes. This study enriches our knowledge about the ecological impacts of salinization and eutrophication in plateau lakes.
Subject(s)
Cyanobacteria , Microbiota , Tibet , Lakes/chemistry , Salinity , ChinaABSTRACT
Trace metals and metalloids in aquatic ecosystems may lead to adverse effects on the survival of fish, especially in the sensitive life stages of vulnerable species. It is still unknown whether threatened fish species in the heavily sediment-laden Yellow River are exposed to toxicity risks associated with multiple trace metals. Herein, we analyzed the concentrations of trace metals in aquatic environmental media and fish tissues across the Yellow River mainstream and assessed the level of metal toxicity to threatened fish. Significantly different concentrations of trace metals in fish tissues were measured between at least two categories among near-threatened, vulnerable, endangered, and critically endangered fish. No metal showed a higher concentration in demersal fish than in pelagic fish. Substantially low metal toxicity was observed for the gill of Rhinogobio nasutus (near-threatened) in the upper reaches, as well as for the gill and liver of Silurus lanzhouensis (endangered) in the middle reaches. High contents of suspended sediment in water and high metal concentrations in sediment and suspended matter could influence the survival and reproduction of fish, especially those already with threatened status.
Subject(s)
Cypriniformes , Metals, Heavy , Trace Elements , Water Pollutants, Chemical , Animals , Metals, Heavy/toxicity , Metals, Heavy/analysis , Rivers , Ecosystem , Water Pollutants, Chemical/toxicity , Water Pollutants, Chemical/analysis , Environmental Monitoring , Geologic SedimentsABSTRACT
The health risk caused by metal pollution is a global concern due to potential metal bioaccumulation, toxicity, and carcinogenicity with multiple sources and pathways. Here, the factors influencing metal bioaccumulation in more than a thousand fish individuals were investigated along a 5464 km continuum of the heavily sediment-laden Yellow River and the health risks to humans were evaluated. The average concentrations of Cr, Zn, As, Cu, Cd, and Pb were below the permissible limits established by domestic and foreign organizations. The fish showed biomagnification of Se and Sn through trophic transfer and a growth dilution effect for V, Mn, Co, Ni, Cu, Zn, As, Cd, and Ba. The concentrations and distributions of most metals in fish were mainly influenced by the trophic levels (δ15N) of fish and the content of the metals in the aquatic environment. The consumption of fish from the Yellow River does not pose a noncarcinogenic risk to the health of adults and juveniles. Cr and As could cause carcinogenic risks, and Cd and Pb also have carcinogenic risks, but these were within an acceptable range. The carcinogenic risks of fish consumption were relatively low in regions with low levels of metal pollution, such as the source region, while the risks were high in regions with heavy pollution and carnivorous fish at high trophic levels. In response to this threat, people can minimize these risks by adjusting their diet and appropriately reducing their consumption of aquatic products from the Yellow River.
Subject(s)
Metals, Heavy , Water Pollutants, Chemical , Animals , Humans , Rivers , Environmental Monitoring , Metals, Heavy/analysis , Bioaccumulation , Cadmium/metabolism , Lead/metabolism , Water Pollutants, Chemical/analysis , Fishes/metabolism , Risk Assessment , ChinaABSTRACT
The Yellow River is one of the largest contributors to the global riverine sediment flux from the land to the ocean. Tissue-specific bioaccumulation of trace metals in fish from heavily sediment-laden rivers remains unclear to date. The concentrations and distributions of trace metals in water, suspended matters, sediments, and various fish tissues were investigated in the mainstem of the Yellow River were investigated. The concentrations of most metals in abiotic media were high in the Gan-Ning-Meng of upstream and downstream segments, and were highest in fine-sized suspended matters. The highest concentrations of most metals were in the gill and liver, followed by the gonad, and lowest in the muscle, and there were a significant overall differences among the tissues. The concentrations of metals in some tissues (e.g., muscle and gill) significantly differed among regions and feeding habits. The highest values of the bioaccumulation factor for suspended matters (BFSPM) were observed in the midstream region (e.g., reaching to 19.0 for Se in the liver). This was determined by metal type and tissue specificity, food composition, and concentration of metals in abiotic media. The results highlight the significance of suspended matters for the distribution of trace metals in abiotic and biotic media.
Subject(s)
Metals, Heavy , Water Pollutants, Chemical , Animals , Bioaccumulation , China , Environmental Monitoring , Geologic Sediments , Metals, Heavy/analysis , Rivers , Water Pollutants, Chemical/analysisABSTRACT
Conservation of fish resources is the key to ecological protection and high-quality development of Yellow River basin. From 1960 to 2019, Yellow River basin distributed 201 fish species, belonging to 16 orders, 35 families. The species number of Cypriniformes was the largest (accounting for 60.7%), followed by Perciformes (accounting for 10.0%). From 1960 to 1980, there were 182 fish species belonging to 15 orders, 28 families. During 1980-2019, there were 112 species, belonging to 10 orders, 23 families. The total number of fish species in source area, midstream and downstream decreased significantly, while that in the upper reaches increased slightly. Jaccard's similarity index of source area, upstream, midstream and downstream between two periods were 34.2%, 46.0%, 42.4% and 35.7%, respectively. Based on feeding preference characteristic, fish species could be divided into eight feeding guilds: phytobenthivores, herbivores, phytoplanktivores, zooplanktivores, omnivorous, insectivores, zoobenthivores, and piscivores. Compared with the period from 1960 to 1980, the proportion of insectivores decreased significantly in the Yellow River basin during 1980-2019, while that of phytobenthivores, herbivores, phytoplanktivores, omnivorous and piscivores increased significantly. From 1960 to 1980, the proportion of insectivores was higher than other reaches at source area and upstream, then began to decrease along the river continuum from reaches with elevation of 2000-3000 m; while the proportion of piscivores was lower than other reaches at source area and upstream, then began to increase along the river continuum from reaches with elevation of 2000-3000 m. From 1980 to 2019, the proportion of insectivores decreased along river continuum from source area, and that of piscivores increased from source area to midstream but decreased in downstream. Development of cascade hydropower, water pollution, insufficient water flow, overfishing and invasion of alien fish were important factors causing the spatiotemporal variations of fish feeding guilds in Yellow River basin.
Subject(s)
Conservation of Natural Resources , Fisheries , Animals , China , Fishes , Humans , RiversABSTRACT
Chromophoric dissolved organic matter (CDOM) in rivers is mainly affected by natural conditions and human activities and can reflect the watershed pollution status to a certain extent. The Yellow River is one of the largest contributors to the global riverine sediment flux from the land to ocean, and there is a paucity of information on how the optical properties of CDOM have the potential to serve as an indicator of water quality for the sediment-laden Yellow River. In this study, a three-dimensional fluorescence parallel factor (PARAFAC) analysis method was applied to investigate the seasonal and spatial variations in CDOM fluorescence components and spectral characteristics from the source region to the estuary in the mainstream of Yellow River. The relationships of CDOM with water quality indicators and trophic state were also analyzed. Six PARAFAC components (C1-C6) were identified and grouped into two categories: humic-like components (C1-C4), which accounted for 85.8 %, and protein-like components (C5 and C6), which accounted for only 14.2 %. The CDOM components, spectral parameters, and their clear correlations with the main ions (Na+ and Cl-) all indicated that the humic-like components may be primarily derived from nonpoint source erosion, and the protein-like components were mainly derived from point source discharges in the watershed. The combination of the CDOM absorption coefficient at 254 nm (a(254)), spectral slope ratio (SR), specific UV absorbance SUVA254, and fluorescence index (FI) had a good predictive ability for the key water quality indicators (total nitrogen (TN), dissolved total nitrogen (DTN), total phosphorus (TP), dissolved total phosphorus (DTP), and chlorophyll a (Chl a)) and trophic state index (TSI). Therefore, some fluorophores and UV spectral parameters of CDOM in the Yellow River can be used for rapid water quality monitoring and pollution source indication, especially pollutants related to nitrogen and phosphorus nutrients in the basin.
Subject(s)
Rivers , Water Quality , China , Chlorophyll A , Environmental Monitoring , Humans , Spectrometry, FluorescenceABSTRACT
The source regions of the Yangtze and Yellow Rivers on the Qinghai-Tibet Plateau are extremely important water resources and ecological functional areas in China, and the ecological environment is fragile and sensitive to climate change. Chromophoric dissolved organic matter (CDOM) is an important component that plays a crucial role in the biogeochemical cycle in aquatic ecosystems. However, knowledge of the distribution characteristics of CDOM in this area is limited. In this study, the optical properties, possible sources of CDOM, and their relationships with environmental variables were investigated in the two regions. The results indicated that the CDOM absorption spectra of these two source regions had a high degree of consistency, and the absorption coefficient aCDOM(355) was small, with a mean of 2.07 ± 1.10 m-1. Two fluorescence components (C1 and C2) were identified and grouped into the humic-like component with parallel factor analysis (PARAFAC) of fluorescence excitation-emission matrices (EEMs), which exhibited highly similar (excitations/emission)max positions between each pair of components in the two regions. Comprehensive CDOM spectral absorption and fluorescence parameters suggested that CDOM was mainly derived from externally input humus, and the source region of the Yellow River showed stronger allochthonous sources. The dissolved organic carbon (DOC) gradients in the water affected the fluorescence intensity and indicated that the humic-like component was an important component of DOC. Water temperature (WT) and turbidity (Turb) positively affected the concentration of CDOM and the ability to absorb light in the aquatic ecosystems. Due to global warming, the rising temperature may lead to an increase in meltwater inflow in the source area and will also bring more external inputs through the runoff.
Subject(s)
Environmental Monitoring , Rivers , China , Ecosystem , Spectrometry, Fluorescence , TibetABSTRACT
In order to study the physiological response to heavy metals and organic-phosphorus pesticide toxicity of aquatic organisms, Neocaridina denticulate was used as a test organism to investigate the impact of physiological indices of N. denticulate muscle tissues when they were exposed to Cu2+ and chlorpyrifos for 5 days respectively with the test methods of semi-static toxicity. The results showed that: when exposed to different concentrations of Cu2+ and chlorpyrifos solutions, the protein concentrations in muscle tissues were significantly lower with the extension of time to varying degrees. In the lower concentration groups of Cu2+ (0.086 mg x L(-1) and 0.172 mg-L-') and the higher concentration groups of chlorpyrifos (0. 006 0 [g-L-' and 0.012 0 µg x L(-1)), the total SOD activity showed inhibitory effect; the trend of the higher concentration group of Cu2+ (0.344 mg x L(-1) and 0.688 mg x L(-1)) showed " inhibition-promotion-inhibition", however, the lower concentration groups of chlorpyrifos (0.001 5 µg x L(-1) and 0.003 0 µg x L(-1)) showed the" inhibition-promotion" changes in trends; MDA contents changed similarly, and within a certain range of concentrations, MDA contents presented a gradually rising trend with increasing Cu2+ and chlorpyrifos concentration, which indicated that Cu2+ and chlorpyrifos accelerated lipid, peroxidation in muscle tissues of N. denticulate. In addition, AChE activity in Cu2+ and chlorpyrifos solutions showed inhibitory effect, and in the solutions with higher concentration of Cu2+ and chlorpyrifos, the activity was gradually decreased with the increase of concentration, indicating that Cu2+ and chlorpyrifoscs impacted the normal physiological functions of N. denticulate, and the higher the concentration, the greater the damage effect. Based on the analysis results, we confirmed that the total SOD, MDA and AChE played significant roles as physiological indicators in evaluating toxic effect of heavy metals and organic-phosphorus pesticide on N. denticulate.