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Chemical synthesis of natural and azido-modified UDP-rhamnose and -arabinofuranose for glycan array-based characterization of plant glycosyltransferases.
Pasini, Irene; Ruprecht, Colin; Osswald, Uwe; Bittmann, Andreas; Maltrovsky, Lina; Romanò, Cecilia; Clausen, Mads H; Pfrengle, Fabian.
Chem Commun (Camb) ; 60(70): 9368-9371, 2024 Aug 27.
Article in English | MEDLINE | ID: mdl-39135501

ABSTRACT

Chemical syntheses of UDP-rhamnose and UDP-arabinofuranose and respective azido-modified analogues are reported. The prepared substrates are useful for the glycan array-based analysis of glycosyltransferases, as exemplified with the plant cell wall-biosynthetic enzymes PvXAT3, AtRRT4 and PtRRT5.


Subject(s)
Glycosyltransferases , Polysaccharides , Uridine Diphosphate Sugars , Glycosyltransferases/metabolism , Glycosyltransferases/chemistry , Polysaccharides/chemistry , Polysaccharides/chemical synthesis , Polysaccharides/metabolism , Uridine Diphosphate Sugars/chemistry , Uridine Diphosphate Sugars/metabolism , Azides/chemistry , Arabinose/chemistry , Arabinose/analogs & derivatives , Plants/chemistry
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