An Update of Lectins from Marine Organisms: Characterization, Extraction Methodology, and Potential Biofunctional Applications.

Lectins are a unique group of nonimmune carbohydrate-binding proteins or glycoproteins that exhibit specific and reversible carbohydrate-binding activity in a non-catalytic manner. Lectins have diverse sources and are classified according to their origins, such as plant lectins, animal lectins, and fish lectins. Marine organisms including fish, crustaceans, and mollusks produce a myriad of lectins, including rhamnose binding lectins (RBL), fucose-binding lectins (FTL), mannose-binding lectin, galectins, galactose binding lectins, and C-type lectins. The widely used method of extracting lectins from marine samples is a simple two-step process employing a polar salt solution and purification by column chromatography. Lectins exert several immunomodulatory functions, including pathogen recognition, inflammatory reactions, participating in various hemocyte functions (e.g., agglutination), phagocytic reactions, among others. Lectins can also control cell proliferation, protein folding, RNA splicing, and trafficking of molecules. Due to their reported biological and pharmaceutical activities, lectins have attracted the attention of scientists and industries (i.e., food, biomedical, and pharmaceutical industries). Therefore, this review aims to update current information on lectins from marine organisms, their characterization, extraction, and biofunctionalities.

Chemical composition and pharmacological activities of Pisum sativum.

BACKGROUND: Consumption of vegetables has been proven to be effective in the prevention of different diseases. Traditionally edible aerial part of Pisum sativum L. subsp. sativum (Fabaceae) is used to treat diabetes, heart diseases and as blood purifier. Present study was aimed to explore the traditional use of aerial parts of P. sativum as a source of antidiabetic agent. In addition, antioxidant activity and chemical composition was carried out. METHODS: Total polyphenol content was spectrophotometrically determined using Folin Chiocalteu's reagent while the flavonoids by aluminum chloride colorimetric assay. Identification of compounds of the extract was made through HPLC and LCMS. Antihyperglycemic activity was assessed by oral glucose tolerance test in mice. Antioxidant activity was determined by DPPH free radical scavenging and reducing power assay. RESULTS: Total polyphenol and total flavonoids content were found to be 51.23 mg gallic acid equivalent and 30.88 mg quercetin equivalent per gram of dried plant extract respectively. Ellagic acid and p-coumeric acid were detected through HPLC. A total of eight compounds including naringenin, ß-sitosterol were indentified through LCMS. In OGTT, extract (200 mg/kg bw) showed a 30.24% decrease (P< 0.05) in blood glucose levels at 30 min compared to the normal control. The extract showed IC50 value of 158.52 µg/mL in DPPH scavenging assay and also showed comparable reducing power. CONCLUSION: Along with other compounds ellagic acid and ß-sitosterol present in the extract may be responsible for its antioxidant as well as antihyperglycemic activities. Altogether these results rationalize the use of this vegetable in traditional medicine.

Identification of the effective crane hook's cross-section by incorporating finite element method and programming language.

The crane hook is a widely utilized component in several industries for the purpose of lifting things. The crane hook must possess the capacity to withstand the intended load without encountering any complications, hence ensuring the safety of both personnel and the objects being lifted. The process of analysis is crucial for the effective utilization of a crane hook. The primary aim of this study was to determine the most efficient cross-sectional crane hook among five distinct geometric profiles. This was achieved through the application of finite element analysis using Solidworks software. Subsequently, the identified cross-sectional profile was further examined using the Python programming language, taking into account the classical equation of a curved beam. The five cross-sectional shapes seen in the study were circular, rectangular, trapezoidal, I-shaped, and T-shaped. For the purposes of this investigation, the chosen material for each cross-sectional crane hook model was 34CrMo4 steel. Despite the identical boundary constraints imposed on all the chosen cross-sectional crane hook profiles, it was observed that the trapezoidal cross-sectional crane hook exhibited superior performance compared to the others. The trapezoidal cross-sectional crane hook model exhibited a Von Mises stress of 203 MPa, with a corresponding factor of safety of 3.20. Further experimentation was conducted using Python to examine the trapezoidal profile. The results indicated that an increased level of parallelism in the inner side of the trapezoidal shape corresponded to a higher factor of safety. Hence, it is advisable to maintain the trapezoidal cross-sectional profile of the crane hook, with due consideration given to maximizing the length of the inner parallel side. The enhancement of design leads to a decrease in the likelihood of failure and the occurrence of undesirable accidents.

The Enterics for Global Health (EFGH) Shigella Surveillance Study in Bangladesh.

Background: Shigella is an important cause of diarrhea in Bangladeshi children <5 years of age, with an incidence rate of 4.6 per 100 person-years. However, the report was more than a decade old, and data on Shigella consequences are similarly outdated and heterogeneously collected. Methods: Facility-based disease surveillance is planned to be carried out under the Enterics for Global Health (EFGH) Shigella Surveillance Study consortium for 2 years with aims to optimize and standardize laboratory techniques and healthcare utilization and coverage survey, clinical and anthropometric data collection, safety monitoring and responsiveness, and other related activities. The EFGH is a cohesive network of multidisciplinary experts, capable of operating in concert to conduct the study to generate data that will pave the way for potential Shigella vaccine trials in settings with high disease burden. The study will be conducted within 7 country sites in Asia, Africa, and Latin America. Conclusions: We outline the features of the Bangladesh site as part of this multisite surveillance network to determine an updated incidence rate and document the consequences of Shigella diarrhea in children aged 6-35 months, which will help inform policymakers and to implement the future vaccine trials.

Quantifying the Cost of Shigella Diarrhea in the Enterics for Global Health (EFGH) Shigella Surveillance Study.

Background: Comparative costs of public health interventions provide valuable data for decision making. However, the availability of comprehensive and context-specific costs is often limited. The Enterics for Global Health (EFGH) Shigella surveillance study-a facility-based diarrhea surveillance study across 7 countries-aims to generate evidence on health system and household costs associated with medically attended Shigella diarrhea in children. Methods: EFGH working groups comprising representatives from each country (Bangladesh, Kenya, Malawi, Mali, Pakistan, Peru, and The Gambia) developed the study methods. Over a 24-month surveillance period, facility-based surveys will collect data on resource use for the medical treatment of an estimated 9800 children aged 6-35 months with diarrhea. Through these surveys, we will describe and quantify medical resources used in the treatment of diarrhea (eg, medication, supplies, and provider salaries), nonmedical resources (eg, travel costs to the facility), and the amount of caregiver time lost from work to care for their sick child. To assign costs to each identified resource, we will use a combination of caregiver interviews, national medical price lists, and databases from the World Health Organization and the International Labor Organization. Our primary outcome will be the estimated cost per inpatient and outpatient episode of medically attended Shigella diarrhea treatment across countries, levels of care, and illness severity. We will conduct sensitivity and scenario analysis to determine how unit costs vary across scenarios. Conclusions: Results from this study will contribute to the existing body of literature on diarrhea costing and inform future policy decisions related to investments in preventive strategies for Shigella.

Post-traumatic stress disorders and coping strategies of health professionals during COVID-19 pandemic in Bangladesh: findings of a countrywide cross-sectional study.

Background: COVID-19 pandemic imposed a devastating effect on the psychological health of health professionals as they worked nonstop to withstand the hardship of the pandemic. The present study intended to determine the post-traumatic stress disorders (PTSD) and coping strategies among health professionals during the COVID-19 pandemic in Bangladesh. Methods: This country-wide cross-sectional study was conducted from July to December 2021 among 1394 health professionals (596 physicians, 713 nurses, 85 medical technologists) who served COVID-19 patients at the secondary, tertiary, and specialized government healthcare facilities in Bangladesh and completed at least one month after exposure to COVID-19 patient-care. Data were collected through face-to-face interviews using a semi-structured questionnaire and analyzed by SPSS software. All the ethical issues were maintained strictly. Findings: Most of the participants, 877 (62.9%) [95% CI: 60.3-65.5], were female, and 327 (23.5%) [95% CI: 21.3-25.8] developed PTSD. Females (AOR:1.42 [95% CI: 1.083-1.868] p = 0.011), having an elderly family member (AOR:1.515 [95% CI: 1.173-1.956] p = 0.0014), working in specialized hospitals (AOR:2.685 [95% CI: 1.928-3.739] p < 0.001), and working ≥8 hours/day (AOR:1.897 [95% CI: 1.350-2.666] p = 0.0002) had higher odds of developing PTSD. Most of the participants adopted spiritual approaches 96 (29.4%) [24.5-34.6] and distraction by watching TV/YouTube 59 (18.0%) [14.0-22.6] as coping strategies. Interpretation: The study findings would be helpful for health policymakers and managers to develop comprehensive measures for restoring the mental well-being of health professionals by alleviating PTSD induced by a pandemic like COVID-19. Funding: The study got funding from the Directorate General of Medical Education under the Ministry of Health and Family Welfare, Bangladesh.

The distribution and characterisation of microplastics in air, surface water and sediment within a major river system.

Rivers are key pathways for the transfer of microplastics (MP) to marine environments. However, there are considerable uncertainties about the amount of microplastics transported by rivers to the ocean; this results in inaccuracies in our understanding of microplastic quantity and transport by freshwater systems. Additionally, it has been suggested that rivers may represent long-term sinks, with microplastics accumulating in sediment due to their high density or other biological, chemical, and physical factors. The atmosphere is also an important pathway by which airborne microplastics may enter aquatic habitats. Here, we compare for first time microplastics type and concentration in these key environmental mediums (air, water and sediment) along a major river (Ganges), from sea to source to understand 1) the abundance, 2) the spatial distribution, and 3) characteristics. Mean microplastic abundance settling from the atmosphere was 41.12 MP m2 day-1; while concentrations in sediment were 57.00 MP kg-1 and in water were 0.05 MP L-1. Across all sites and environmental mediums, rayon (synthetically altered cellulose) was the dominant polymer (54-82 %), followed by acrylic (6-23 %) and polyester (9-17 %). Fibres were the dominant shape (95-99 %) and blue was the most common colour (48-79 %). Across water and sediment environmental mediums, the number of microplastics per sample increased from the source of the Ganges to the sea. Additionally, higher population densities correlated with increased microplastic abundance for air and water samples. We suggest that clothing is likely to be the prominent source of microplastics to the river system, influenced by atmospheric deposition, wastewater and direct input (e.g. handwashing of clothes in the Ganges), especially in high density population areas. However, we suggest that subsequent microplastic release to the marine environment is strongly influenced by polymer type and shape, with a large proportion of denser microplastics settling in sediment prior to the river discharging to the ocean.

Trophic transfer of microplastics in the aquatic ecosystem of Sundarbans mangrove forest, Bangladesh.

Globally microplastics (MPs) contaminations have been widely reported across the large number of organisms in the marine ecosystem. Consequently, trophic transfer of MPs inferred to occur across the organisms of marine food webs. However, scientific evidence on trophic transfer of MPs across the marine organisms is very limited. Therefore, this study aimed to understand the transfer of MPs across the trophic levels in the marine ecosystem. We sampled individuals of different species of primary consumers, secondary consumers, tertiary consumers and quaternary consumers from the aquatic ecosystem of Sundarbans mangrove forest from June 2021 to December 2021. This study found that marine organisms in the aquatic ecosystem of Sundarbans mangrove forest are contaminated with MPs. The abundance of MPs in collected samples varied between 0.56 ±â€¯0.25 items/individual and 6.06 ±â€¯1.20 items/individual. Maximum MPs was recorded as 5.5 ±â€¯1.21 items/individual in predators followed by 5.1 ±â€¯0.85, 4.5 ±â€¯0.39, 1.2 ±â€¯0.26, 1.1 ±â€¯0.28 and 1.01 ±â€¯0.25 in filter feeders, browsers, deposit feeders, selective planktivores and variable feeders, respectively. Maximum MPs abundance was encountered in quaternary consumers (4.17 items/individual) followed by tertiary consumers (3.17 items/individual), secondary consumers (2.74 items/individual) and primary consumers (0.56 items/individual). We found that MPs abundance increases with the increase of trophic levels (R2 = 0.64, p < 0.001) which indicates that transfer of MPs across different trophic levels and also showed the evidence of biomagnification of MPs in successive trophic levels. Our study is the first report of trophic transfer of MPs in sub-tropical mangrove ecosystem and will serve as a guideline to understand the MPs pollution in the coastal ecosystem of Bangladesh.

The ecological impact of plastic pollution in a changing climate.

Assessing three interlinked issues, plastic pollution, climate change and biodiversity loss separately can overlook potential interactions that may lead to positive or negative impacts on global ecosystem processes. Recent studies suggest that threatened species and ecosystems are vulnerable to both plastic pollution and climate change stressors. Here we consider the connectivity and state of knowledge between these three environmental issues with a focus on the Global South. Nine out of top ten Long-Term Climate Risk Index (CRI) (2000-2019) ranked countries are located within the Global South, yet research is focused in the Global North. A literature search for the top ten Long-Term Climate Risk Index (CRI) (2000-2019) ranked countries matched a total of 2416 (3.3% of global publications) search results on climate change, with 56 (4% of the global publications) on plastic pollution, and seven (7.7% of the global publications) on both climate change and plastic pollution. There is a strong correlation between the Global South and high biodiversity hotspots, high food insecurity and low environmental performance. Using Bangladesh as a case study, we show the erosion rates and sea level rise scenarios that will increase ocean-bound plastic pollution and impact high biodiversity areas. Poverty alleviation and promoting renewable energy and green practices can significantly reduce the stress on the environment. We recommend that these connected planetary threats can be best addressed through a holistic and collaborative approach to research, a focus on the Global South, and an ambitious policy agenda.

Time series analysis and predicting COVID-19 affected patients by ARIMA model using machine learning.

The spread of a respiratory syndrome known as Coronavirus Disease 2019 (COVID-19) quickly took on pandemic proportions, affecting over 192 countries. An emergency of the health system was obligated for the response to this epidemic. Although containment measures in China reduced new cases by more than 90 %, the levels of reduction were not the same in other countries. So, the question that arises is: what the world will see this pandemic, and how many patients can be affected? The response would be helpful and supportive of the authority and the community to prepare for the coming days. In this study, the Autoregressive Integrated Moving Average (ARIMA) model was employed to analyze the temporal dynamics of the worldwide spread of COVID-19 in the time window from January 22, 2020 to April 7, 2020. The cumulative number of confirmed Covid-19-affected patients forecasted over the three months was between 9,189,262 - 14,906,483 worldwide. This prediction value of Covid 19-affected patients will be valid only if the situation remains unchanged, and the epidemic spreads according to the previous nature worldwide in these three months.

In Vivo Bioactivities of Hoya parasitica (Wall.) and In Silico Study against Cyclooxygenase Enzymes.

Hoya parasitica (Wall.) is extensively used in traditional medicine for the treatment of various diseases including rheumatism, kidney problems, jaundice, urinary tract disorders, fever, and pain. The present study was designed to explore new lead compound(s) to alleviate pain, pyresis, and diarrhea from methanol, ethyl acetate, and n-hexane extracts of H. parasitica (Wall.) leaves (MHP, EAHP, and NHP, respectively). Analgesic activity of the extracts was assessed through acetic acid induced writhing, tail immersion, and hot plate tests while brewer's yeast-induced pyrexia test was employed for the assessment of antipyretic activity. Besides, castor oil and magnesium sulfate induced diarrheal tests were utilized for the evaluation of antidiarrheal properties. Moreover, in silico study of the isolated compounds was undertaken to seek out best-fit phytoconstituent(s) against cyclooxygenase enzymes. MHP revealed substantial antioxidant activities in different in vitro assays compared to EAHP and NHP. In the acetic acid-induced writhing test, among the extracts, MHP (400 mg/kg) revealed maximum 74.15 ± 1% inhibition of writhing comparable to that of standard (85.77 ± 1.39%). Again, in tail immersion and hot plate tests, higher doses of all the test samples exhibited a significant increase of latent period in a time-dependent manner. In brewer yeast-induced pyrexia test, at 3rd and 4th hour of treatment, significant (P < 0.05) antipyretic action was found in the test samples. In both castor oil and magnesium induced diarrheal tests, MHP at 400 mg/kg showed the highest percent inhibition of diarrhea (68.62 ± 4.74 and 64.99 ± 2.90, respectively). Moreover, molecular docking analysis corroborated the results of the present study. The findings of the present study supported the traditional uses of this plant for the alleviation of pain and fever. Furthermore, hoyasterone was found to be the most effective lead compound as cyclooxygenase enzyme inhibitor.

Evaluation of in vitro and in silico anti-inflammatory potential of some selected medicinal plants of Bangladesh against cyclooxygenase-II enzyme.

ETHNOPHARMACOLOGICAL RELEVANCE: Medicinal plants are sources of chemical treasures that can be used in treatment of different diseases, including inflammatory disorders. Traditionally, Heritiera littoralis, Ceriops decandra, Ligustrum sinense, and Polyscias scutellaria are used to treat pain, hepatitis, breast inflammation. The present research was designed to explore phytochemicals from the ethanol extracts of H. littoralis, C. decandra, L. sinense, and P. scutellaria to discern the possible pharmacophore (s) in the treatment of inflammatory disorders. MATERIAL AND METHODS: The chemical compounds of experimental plants were identified through GC-MS analysis. Furthermore, in-vitro anti-inflammatory activity was assessed in human erythrocytes and an in-silico study was appraised against COX-2. RESULTS: The experimental extracts totally revealed 77 compounds in GC-MS analysis and all the extracts showed anti-inflammatory activity in in-vitro assays. The most favorable phytochemicals as anti-inflammatory agents were selected via ADMET profiling and molecular docking with specific protein of the COX-2 enzyme. Molecular dynamics simulation (MDS) confirmed the stability of the selected natural compound at the binding site of the protein. Three phytochemicals exhibited the better competitive result than the conventional anti-inflammatory drug naproxen in molecular docking and MDS studies. CONCLUSION: Both experimental and computational studies have scientifically revealed the folklore uses of the experimental medicinal plants in inflammatory disorders. Overall, N-(2-hydroxycyclohexyl)-4-methylbenzenesulfonamide (PubChem CID: 575170); Benzeneethanamine, 2-fluoro-. beta., 3, 4-trihydroxy-N-isopropyl (PubChem CID: 547892); and 3,5-di-tert-butylphenol (PubChem CID: 70825) could be the potential leads for COX-2 inhibitor for further evaluation of drug-likeliness.

Metabolite profiling, anti-inflammatory, analgesic potentials of edible herb Colocasia gigantea and molecular docking study against COX-II enzyme.

ETHNOPHARMACOLOGICAL RELEVANCE: Consumable herbs play a basic part in sustenance and human health. Traditionally, Colocasia gigantea Hook (Araceae) is used to treat fever, infection, wounds healing, drowsiness, tuberculosis, stomach problems etc. AIM OF THE STUDY: The study aspired to identify bioactive compounds, to evaluate anti-inflammatory and analgesic potentials of edible herb C. gigantea, and to molecular docking study against anti-inflammatory enzyme Cyclooxygenase-2 (COX-2). MATERIALS AND METHODS: Chemical components of C. gigantea were discerned by HPLC and GCMS assays. In vitro anti-inflammatory activity was appraised by heat-induced, hypotonicity, and hydrogen peroxide-induced hemolysis assays and in vivo by formalin-induced paw edema assay. In vivo analgesic activity was evaluated by acetic acid-induced pain modulation assay. Also, molecular docking of the identified compounds was explored against the anti-inflammatory enzyme cyclooxygenase-2. RESULTS: HPLC-DAD analysis divulged the presence of trans-cinnamic acid along with (-)-epicatechin as a prime component. Also, 9, 12-Octadecadienoic acid (37.86%) and n-Hexadecanoic acid (25.89%) as the major as well as 24 other compounds were confirmed through GCMS in the extract. In in vitro anti-inflammatory study, C. gigantea extract indicated prominent erythrocyte membrane stabilization activity with good percentage aegis in all experimental assays. In addition to, formalin-induced in vivo anti-inflammatory assay revealed the maximum (42.37% and 48.72%) suppression of edema at the fourth hour at 250 and 500 mg/kg body weight, respectively. Moreover, an in-vivo pain modulation assay exposed significant (p < 0.05) activity at experimental doses. Furthermore, in the docking study, (-)-epicatechin was more active rather than other identified compounds with strong binding affinity to COX-2 protein. CONCLUSIONS: The extract evinced remarkable anti-inflammatory and analgesic activities. Identified bioactive components along with other components of the extract might play a pivotal role in the observed bioactivity and the results vindicate the use of edible herb C. gigantea in ancestral medicine.

Plastic pollution in aquatic systems in Bangladesh: A review of current knowledge.

Rivers play a crucial role in transporting land-based plastic waste to the ocean, with the Ganges reported as the second largest contributing river of plastic pollution globally. To better quantify global plastic pollution transport and effectively reduce the sources and risks imposed, a clear understanding of the origin, transport, fate, and effects of riverine plastic debris is important. In this review paper, we discuss the current state of knowledge of plastic pollution in aquatic systems in Bangladesh and evaluate existing research gaps. Bangladesh has been recognized as an internationally significant nation in the plastic pollution crisis, but this paper identifies a major disconnect in knowledge, understanding and capacity to understand and address this critical environmental and public health issue. Here, we review all available scientific publications on plastic pollution in the freshwater and marine environment in Bangladesh and identify key research themes. A total of 24 studies relevant to plastic pollution were published from 2006 to 2019, of which 18 were selected for this study under the authors' criteria. Nine focused on plastic pollution in the marine environment, eight focused on plastic waste generation and management and only one focused on the freshwater environment. We compared our findings with three other countries in the Global South with comparable per capita gross domestic product (GDP) and mismanaged waste, namely Cambodia, Kenya, and Tanzania, revealing similar knowledge gaps. This lack of research demonstrates a need for further work to monitor and model riverine plastic transport and examine the implications for aquatic organisms. This will facilitate the formulation of national management strategies aimed at addressing plastic pollution.

Pandemics, vulnerability, and prevention: time to fundamentally reassess how we value and communicate risk?: CITIES, HEALTH and COVID-19: Initial reflections and future challenges.

For over a decade, pandemics have been on the UK National Risk Register as both the likeliest and most severe of threats. Non-infectious 'lifestyle' diseases were already crippling our healthcare services and our economy. COVID-19 has exposed two critical vulnerabilities: firstly, the UK's failure to adequately assess and communicate the severity of non-communicable disease; secondly, the health inequalities across our society, due not least to the poor quality of our urban environments. This suggests a potentially disastrous lack of preventative action and risk management more generally, notably with regards to the existential risks from the climate and ecological crises.

Riverine plastic pollution from fisheries: Insights from the Ganges River system.

Abandoned, lost or otherwise discarded fishing gear represents a substantial proportion of global marine plastic pollution and can cause significant environmental and socio-economic impacts. Yet little is known about its presence in, and implications for, freshwater ecosystems or its downstream contribution to plastic pollution in the ocean. This study documents fishing gear-related debris in one of the world's largest plastic pollution contributing river catchments, the Ganges. Riverbank surveys conducted along the length of the river, from the coast in Bangladesh to the Himalaya in India, show that derelict fishing gear density increases with proximity to the sea. Fishing nets were the main gear type by volume and all samples examined for polymer type were plastic. Illegal gear types and restricted net mesh sizes were also recorded. Socio-economic surveys of fisher communities explored the behavioural drivers of plastic waste input from one of the world's largest inland fisheries and revealed short gear lifespans and high turnover rates, lack of appropriate end-of-life gear disposal methods and ineffective fisheries regulations. A biodiversity threat assessment identified the air-breathing aquatic vertebrate species most at risk of entanglement in, and impacts from, derelict fishing gear; namely species of threatened freshwater turtle and otter, and the endangered Ganges river dolphin. This research demonstrates a need for targeted and practical interventions to limit the input of fisheries-related plastic pollution to this major river system and ultimately, the global ocean. The approach used in this study could be replicated to examine the inputs, socio-economic drivers and ecological impacts of this previously uncharacterised but important source of plastic pollution in other major rivers worldwide.

Measuring Process Capability in a Hospital by Using Lean Six Sigma Tools-A Case Study in Bangladesh.

Bangladesh's government had been constantly conscious of its health-care system. As a result, the health-care system had seen a significant rise in quality in the past few years. The Lean Six Sigma concept is used to boost up the productivity as well as the quality of products or services. The purpose of this article was to measure the process capability in the health-care system by lean tools. In this study, we selected the dialysis unit as our investigation area where we applied the Six Sigma DMAIC (Define, Measure, Analysis, Improve, and Control) model which had been structured step by step, respectively. Furthermore, different tools of Total Quality Management, Statistical Quality Control and Lean manufacturing like SIPOC (Supplier, Input, Process, Output, and Customer) diagram, P Control chart, Fishbone diagram, and Pareto analysis had been performed in different phases of the DMAIC model. Measuring process capability using the DMAIC model helped to identify the problems associated with the dialysis unit and also recommendations were developed based on investigation and analysis. These recommendations will help the hospital management to overcome all problems and increase service rate and patient safety in the dialysis unit.

TP53 codon 72 polymorphism and breast cancer risk in Bangladeshi population.

BACKGROUND: Breast cancer, a hereditary or heterogeneous sporadic disease, is the most common cancer in women worldwide. The tumor suppressor TP53 gene has been found to be the most commonly mutated genes in many types of human cancers, including breast cancer. This study aimed to investigate the association of codon 72 polymorphism of TP53 gene with breast cancer risk in Bangladeshi females. METHODS: The study included 125 cases and 125 healthy controls. Genotyping and polymorphism were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. RESULTS: The frequencies of the three genotypes Arg/Arg, Arg/Pro, and Pro/Pro were 43.2, 33.6, and 23.2% in cases, whereas 48.8, 40.8, and 10.4% in controls, respectively. The frequency of mutant homozygous (Pro/Pro) genotype was significantly increased in breast cancer patients as compared with controls (23.2 vs 10.4%), and showed 2.52-fold significantly increased risk for breast cancer (OR 2.5199, 95% CI 1.19-5.33, p = 0.0157). The frequencies of Pro/Pro genotype were significantly higher in breast cancer cases with non-breast feeding status. Pro allele frequency was found to be significantly increased in breast cancer cases (OR 1.4978, 95% CI 1.0357-2.1662, p = 0.0318). CONCLUSIONS: Our data suggest that mutant (Pro/Pro) homozygosity at codon 72 of TP53 gene is significantly associated with breast cancer susceptibility in Bangladeshi women. In addition, this association was significantly related to lactating status.