Genetic diversity of Rhizobium from nodulating beans grown in a variety of Mediterranean climate soils of Chile.

In spite of potentially being an important source of rhizobial diversity and a key determinant of common bean productivity, there is a paucity of data on Rhizobium genetic variation and species composition in the important bean producing area of Chile and only one species has been documented (Rhizobium leguminosarum). In this study, 240 Rhizobium isolates from Torcaza bean (Phaseolus vulgaris L.) nodules established in the highest bean producing area in Chile (33°34'S-70°38'W and 37°36'S-71°47'W) were characterized by PCR-RFLP markers for nodC gene, revealing eight banding patterns with the polymorphic enzyme Hinf I. The locality of San Agustín de Aurora in Central Chile (35°32'S-71°29'W) had the highest level of diversity. Isolates were classified by species using PCR-RFLP markers for 16S rDNA gene and were confirmed by sequencing an internal fragment of the 16S rDNA gene. The results confirmed the presence of R. leguminosarum and three other species of rhizobia nodulating beans in South Central Chile (R. etli, R. tropici and R. leucaenae). R. tropici and R. leucaenae showed the least genetic variation and were most commonly identified in acid soils, while R. etli was the most common species in slightly acidic to moderately alkaline soils, with higher levels of organic matter content. R. leguminosarum was identified in almost all soils, was the most genetically diverse, and was the most common, being documented in soils with pH that ranged between 5.3 and 8.2, and with organic matter content between 2.1 and 4 %.

Microbial impact on initial soil formation in arid and semiarid environments under simulated climate change.

The microbiota is attributed to be important for initial soil formation under extreme climate conditions, but experimental evidence for its relevance is scarce. To fill this gap, we investigated the impact of in situ microbial communities and their interrelationship with biocrust and plants compared to abiotic controls on soil formation in initial arid and semiarid soils. Additionally, we assessed the response of bacterial communities to climate change. Topsoil and subsoil samples from arid and semiarid sites in the Chilean Coastal Cordillera were incubated for 16 weeks under diurnal temperature and moisture variations to simulate humid climate conditions as part of a climate change scenario. Our findings indicate that microorganism-plant interaction intensified aggregate formation and stabilized soil structure, facilitating initial soil formation. Interestingly, microorganisms alone or in conjunction with biocrust showed no discernible patterns compared to abiotic controls, potentially due to water-masking effects. Arid soils displayed reduced bacterial diversity and developed a new community structure dominated by Proteobacteria, Actinobacteriota, and Planctomycetota, while semiarid soils maintained a consistently dominant community of Acidobacteriota and Proteobacteria. This highlighted a sensitive and specialized bacterial community in arid soils, while semiarid soils exhibited a more complex and stable community. We conclude that microorganism-plant interaction has measurable impacts on initial soil formation in arid and semiarid regions on short time scales under climate change. Additionally, we propose that soil and climate legacies are decisive for the present soil microbial community structure and interactions, future soil development, and microbial responses.

CLSoilMaps: A national soil gridded database of physical and hydraulic soil properties for Chile.

Spatially explicit soil information is crucial for comprehending and managing many of Earth´s processes related to carbon, water, and other biogeochemical cycles. We introduced a gridded database of soil physical properties and hydraulic parameters at 100 meters spatial resolution. It covers the continental area of Chile and binational basins shared with Argentina for six standardized depths following the specifications of the GlobalSoilMap project. We generated soil maps based on digital soil mapping techniques based on more than 4000 observations, including unpublished data from remote areas. These maps were used as input for the pedotransfer function Rosetta V3 to obtain predictions of soil hydraulic properties, such as field capacity, permanent wilting point, total available water capacity, and other parameters of the water retention curve. The trained models outperformed several other DSM studies applied at the national and regional scale for soil physical properties (nRMSE ranging from 6.93% to 15.7%) and delivered acceptable predictions (nRMSE ranging from 10.4% to 15.6%) for soil hydraulic properties, making them suitable for countless environmental studies.

Impact of Climate and Slope Aspects on the Composition of Soil Bacterial Communities Involved in Pedogenetic Processes along the Chilean Coastal Cordillera.

Soil bacteria play a fundamental role in pedogenesis. However, knowledge about both the impact of climate and slope aspects on microbial communities and the consequences of these items in pedogenesis is lacking. Therefore, soil-bacterial communities from four sites and two different aspects along the climate gradient of the Chilean Coastal Cordillera were investigated. Using a combination of microbiological and physicochemical methods, soils that developed in arid, semi-arid, mediterranean, and humid climates were analyzed. Proteobacteria, Acidobacteria, Chloroflexi, Verrucomicrobia, and Planctomycetes were found to increase in abundance from arid to humid climates, while Actinobacteria and Gemmatimonadetes decreased along the transect. Bacterial-community structure varied with climate and aspect and was influenced by pH, bulk density, plant-available phosphorus, clay, and total organic-matter content. Higher bacterial specialization was found in arid and humid climates and on the south-facing slope and was likely promoted by stable microclimatic conditions. The presence of specialists was associated with ecosystem-functional traits, which shifted from pioneers that accumulated organic matter in arid climates to organic decomposers in humid climates. These findings provide new perspectives on how climate and slope aspects influence the composition and functional capabilities of bacteria, with most of these capabilities being involved in pedogenetic processes.

Effect of the Soil Matric Potential on the Germination Capacity of Prosopis chilensis, Quillaja saponaria and Cryptocarya alba from Contrasting Geographical Origins.

As a consequence of the megadrought in Central Chile, it is expected that most of the distribution of woody species will be narrowed in the northern limits because of restrictions imposed by soil matric potential on seed germination. In this study, we analyzed the effect of the soil matric potential on seed germination and initial recruitment of the sclerophyllous species Prosopis chilensis, Quillaja saponaria and Cryptocarya alba from contrasting geographic origins (i.e., seed sources). We evaluated the germination capacity (%) under different matric potentials (i.e., 0, -6, -33, -750 and -1250 kPa) for 100 days. Soil matric potential of -1250 kPa negatively affected the germination capacity of the three species. P. chilensis seeds stopped germinating under soil matric potential close to -1200 kPa, whereas in Q. saponaria and C. alba the complete inhibition of germination was under -1000 kPa. Seed sources also differed in their germination capacity by soil matric potential: northern seed sources of P. chilensis germinated with the lowest soil matric potential. There was no clear trend in Q. saponaria and C. alba, but in general, southern seed sources performed better than the northern ones. The results showed that Ñ°m in the soil played an important role in the germinative capacity against different seed source origins, but not in soils with a north-south gradient.

Pesticide risk management using indicators for vineyards in the central valley of Chile.

The Apalta catchment is a wine-producing area of 1300 ha, lying south of Santiago, located in the Colchagua Valley, near Santa Cruz in the VI Region of Chile. The vineyards are planted on slopes of up to 22 degrees, the rows being aligned mainly down the slope. This cropping system and its management lead to a potential contamination of natural resources. To assess the risk of environmental contamination due to the application of pesticides in different pest control strategies, indicators at a field level were derived according to a step-by-step procedure. Using these indicators, runoff was found to be the main process of impact. The different pest control strategies in this catchment caused different potential risks, though these in general were low.