Entanglement Assisted Probe of the Non-Markovian to Markovian Transition in Open Quantum System Dynamics.

We utilize a superconducting qubit processor to experimentally probe non-Markovian dynamics of an entangled qubit pair. We prepare an entangled state between two qubits and monitor the evolution of entanglement over time as one of the qubits interacts with a small quantum environment consisting of an auxiliary transmon qubit coupled to its readout cavity. We observe the collapse and revival of the entanglement as a signature of quantum memory effects in the environment. We then engineer the non-Markovianity of the environment by populating its readout cavity with thermal photons to show a transition from non-Markovian to Markovian dynamics, ultimately reaching a regime where the quantum Zeno effect creates a decoherence-free subspace that effectively stabilizes the entanglement between the qubits.

Parity-Time Symmetric Holographic Principle.

Originating from the Hamiltonian of a single qubit system, the phenomenon of the avoided level crossing is ubiquitous in multiple branches of physics, including the Landau-Zener transition in atomic, molecular, and optical physics, the band structure of condensed matter physics and the dispersion relation of relativistic quantum physics. We revisit this fundamental phenomenon in the simple example of a spinless relativistic quantum particle traveling in (1+1)-dimensional space-time and establish its relation to a spin-1/2 system evolving under a PT-symmetric Hamiltonian. This relation allows us to simulate 1-dimensional eigenvalue problems with a single qubit. Generalizing this relation to the eigenenergy problem of a bulk system with N spatial dimensions reveals that its eigenvalue problem can be mapped onto the time evolution of the edge state with (N-1) spatial dimensions governed by a non-Hermitian Hamiltonian. In other words, the bulk eigenenergy state is encoded in the edge state as a hologram, which can be decoded by the propagation of the edge state in the temporal dimension. We argue that the evolution will be PT-symmetric as long as the bulk system admits parity symmetry. Our work finds the application of PT-symmetric and non-Hermitian physics in quantum simulation and provides insights into the fundamental symmetries.

Hyperactive DNA Cutting for Unbiased UHPLC-MS/MS Quantification of Epigenetic DNA Marks by Engineering DNase I Mutants.

Epigenetic DNA modifications, such as 5-methylcytosine, 5-hydroxymethylcytosine, and 5-formylcytosine, are associated with a variety of diseases and potential biomarkers for cancer diagnosis and therapy. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays are considered to be the gold standard for qualitative and quantitative detection of DNA modifications. DNA digestion for converting long DNA polymer into 2'-deoxynucleosides is an important preprocessing step to achieve sensitive and accurate LC-MS/MS quantification. Here, we showed that, as stimulated by divalent metal ions, Mg2+ and Mn2+, the engineered human DNase I Q9R:E13R:N74K mutant can efficiently digest DNA in the presence of monovalent metal ions at a high concentration (e.g., 1 M NaCl), showing hyperactivity on DNA cutting. We also found that the engineered DNase I mutants display exceptional DNA-cutting activity over a wider pH range (5.5-9.5). Due to their hyperactivity and high salt tolerance, the engineered DNase I mutants cut DNA 5mC and dC efficiently. Benefitting from this DNA-cutting hyperactivity, we demonstrated an LC-MS/MS assay for unbiased and accurate quantification of DNA 5mC.

Zero-Interfacing µHPLC to ICPMS.

The chromatography-mass spectrometry hyphenated technique is the most widely adopted tool for quantifying trace analytes in a complex biosample. One issue we frequently encountered, however, is that the separated analyte-containing chromatographic peaks broaden and even remix prior to mass spectrometric quantification due to the inevitable molecular diffusion within the dead-volume introduced by hyphenation. We developed a zero-interfacing approach for coupling microbore (µ) HPLC with inductively coupled plasma mass spectrometry (ICPMS). Zero-interfacing µHPLC to ICPMS has been achieved by a column-nebulizer assembly (COL-NEB) of a self-designed glass framework with a tapered nozzle, in which a capillary chromatographic column can be harbored while an Ar gas flow is blown through the nozzle mouth. The COL-NEB can be positioned just before the base of the Ar-ICP serving as the central sampling channel of a conventional Ar-ICP torch for online nebulization and transportation of the analytes separated on µHPLC into ICPMS, maintaining the molecular resolution obtained on µHPLC and the limit of detection (LOD) of ICPMS. For example, the full width at half-maximum of a SLUGT peptide chromatographic peak was reduced to 1.71 ± 0.07 s (n = 5) with a 0.72 fg LOD (3σ) of 80Se. Moreover, at least 32 Se-containing peptides were determined in the trypsin lysate of the water-soluble fraction (≥3000 MW) from Se-enriched yeast CRM SELM-1 within a 10 min run, the highest record to date. We believe such an approach paves the way to determining accurate information on a heteroatom and its binding biomolecules that play key roles during life processes.

Context-dependent regulation of Notch signaling in glial development and tumorigenesis.

In the mammalian brain, Notch signaling maintains the cortical stem cell pool and regulates the glial cell fate choice and differentiation. However, the function of Notch in regulating glial development and its involvement in tumorigenesis have not been well understood. Here, we show that Notch inactivation by genetic deletion of Rbpj in stem cells decreases astrocytes but increases oligodendrocytes with altered internal states. Inhibiting Notch in glial progenitors does not affect cell generation but instead accelerates the growth of Notch-deprived oligodendrocyte progenitor cells (OPCs) and OPC-related glioma. We also identified a cross-talk between oligodendrocytes and astrocytes, with premyelinating oligodendrocytes secreting BMP4, which is repressed by Notch, to up-regulate GFAP expression in adjacent astrocytes. Moreover, Notch inactivation in stem cells causes a glioma subtype shift from astroglia-associated to OPC-correlated patterns and vice versa. Our study reveals Notch's context-dependent function, promoting astrocytes and astroglia-associated glioma in stem cells and repressing OPCs and related glioma in glial progenitors.

Observation of parity-time symmetry breaking in a single-spin system.

Steering the evolution of single spin systems is crucial for quantum computing and quantum sensing. The dynamics of quantum systems has been theoretically investigated with parity-time-symmetric Hamiltonians exhibiting exotic properties. Although parity-time symmetry has been explored in classical systems, its observation in a single quantum system remains elusive. We developed a method to dilate a general parity-time-symmetric Hamiltonian into a Hermitian one. The quantum state evolutions ranging from regions of unbroken to broken [Formula: see text] symmetry have been observed with a single nitrogen-vacancy center in diamond. Owing to the universality of the dilation method, our result provides a route for further exploiting and understanding the exotic properties of parity-time symmetric Hamiltonian in quantum systems.