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A longitudinal proteomic assessment of peptide degradation and loss under acidic storage conditions.
Anal Biochem
; 473: 11-3, 2015 Mar 15.
Article
in English
| MEDLINE
| ID: mdl-25479603
ABSTRACT
Sample preparation prior to analysis by liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) usually involves the storage of frozen peptide samples in an acidic environment for variable time periods. Questions arose in our laboratory regarding the stability of peptides in acid under medium- to long-term storage. Thus, a 10-month longitudinal study was designed to assess the effect on storage of tryptic peptides at -20 and -80°C under acidic conditions. Our conclusion and proposal from this evaluation is that the optimal storage conditions of peptide samples in acid for proteomic experiments is at -80°C and, ideally, as separate aliquots.