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Methods Mol Biol ; 2652: 55-77, 2023.
Article in English | MEDLINE | ID: mdl-37093470

ABSTRACT

The baculovirus expression vector system (BEVS) is one of the most popular eukaryotic systems for recombinant protein production. The focus of our protein production platform is the provision of recombinant proteins for research use, where generally only small quantities are required, in the range of tens of micrograms to a few hundred milligrams. Here, we present methods that reflect our standard operating procedures and setup to be able to frequently, and often repeatedly, produce many different types of proteins.


Subject(s)
Baculoviridae , Genetic Vectors , Baculoviridae/genetics , Recombinant Proteins/metabolism
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