De novo mutation in the DSPP gene associated with dentinogenesis imperfecta type II in a Japanese family.

Dentinogenesis imperfecta (DGI) type II is one of the most common dominantly inherited dentin defects, in which both the primary and permanent teeth are affected. Here, we report a Japanese family with autosomal-dominant DGI type II, including both molecular genetic defects and pathogenesis with histological analysis. Mutation analysis revealed a mutation (c.53T>A, p.V18D, g.1192T>A) involving the second nucleotide of the first codon within exon 3 of the dentin sialophosphoprotein (DSPP) gene. This mutation has previously been reported in a Korean family. Thus far, 24 allelic DSPP mutations have been reported, and this is the seventh mutation involving the DSPP V18 residue. Among those, only one other was shown to be caused by a de novo mutation, and that mutation also affected the V18 amino acid residue. The DSPP V18 residue is highly conserved among other mammalian species. These findings thus suggest that the V18 amino acid might be a sensitive mutational hot spot, playing a critical role in the pathogenesis of DGI.

Effects of tooth-conditioning agents on bond strength of a resin-modified glass-ionomer sealant to enamel.

OBJECTIVES: The purpose of this study was to evaluate the effects of tooth-conditioning agents on bond strength of resin-modified glass-ionomer sealant (Fuji III LC) to bovine enamel as well as on dissolution of calcium ions from the bovine enamel surfaces. METHODS: The enamel surfaces of bovine lower incisors were treated with 10 and 20% polyacrylic acid, 12% citric acid and 35% phosphoric acid for 20s. Fuji III LC was applied to the etched enamel surfaces, and the shear bond strength of each specimen was measured using an Instron Universal Testing Instrument. The amounts of calcium ions dissolved from the treated enamel surfaces were also measured using a polarized Zeeman atomic absorptiometer. RESULTS: In specimens pretreated with distilled water, 10% polyacrylic acid, 20% polyacrylic acid, 12% citric acid and 35% phosphoric acid, the mean values of shear bond strength were 5.5, 12.5, 15.2, 15.2 and 15.1MPa, respectively, and the amounts of Ca(2+) dissolved from the enamel surfaces were 5.6, 41.4, 88.5, 131.6 and 588.3 microg/cm(2), respectively. CONCLUSIONS: The adhesion of a resin-modified glass-ionomer sealant to bovine enamel was significantly improved by the use of tooth-conditioning agents. Especially, treatment of an enamel surface with 20% polyacrylic acid results in good shear both strength and relatively small degree of enamel erosion.