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Positive Streptobacillus moniliformis PCR in guinea pigs likely due to Leptotrichia spp.
Boot, Ron; Van de Berg, Lia; Reubsaet, Frans A G; Vlemminx, Maurice J.
Vet Microbiol ; 128(3-4): 395-9, 2008 Apr 30.
Article in English | MEDLINE | ID: mdl-18023543

ABSTRACT

Streptobacillus moniliformis is a zoonotic bacterium. We obtained positive S. moniliformis PCR results in oral swab samples from guinea pigs from an experimental colony and the breeding colony of origin. Comparison of the DNA sequence of an amplicon with deposited 16S rDNA sequences revealed that Leptotrichia sp. can be the source of a false positive S. moniliformis PCR outcome.


Subject(s)
Fusobacterium Infections/veterinary , Guinea Pigs/microbiology , Leptotrichia/isolation & purification , Polymerase Chain Reaction/veterinary , Rodent Diseases/diagnosis , Streptobacillus/isolation & purification , Animals , Base Sequence , DNA, Bacterial/chemistry , DNA, Bacterial/genetics , DNA, Ribosomal/chemistry , DNA, Ribosomal/genetics , False Positive Reactions , Fusobacterium Infections/diagnosis , Fusobacterium Infections/microbiology , Molecular Sequence Data , Polymerase Chain Reaction/methods , Polymerase Chain Reaction/standards , Rodent Diseases/microbiology , Sequence Alignment/veterinary , Species Specificity
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