Prevention of diet-induced type 2 diabetes in the C57BL/6J mouse model by an antidiabetic herbal formula.

Significant positive effects of an antidiabetes herbal formula (ADHF) on the prevention of progressive diet-induced type 2 diabetes (NIDDM) in the well recognized C57BL/6J mouse model were demonstrated. The results of the present studies confirm that C57BL/6J mice, through a genetic predisposition, developed NIDDM-like symptoms on a regular schedule when fed solely on a diabetes induction diet containing high fat, high simple carbohydrate and low fibre. Blood glucose levels consistent with type 2 diabetes were detected after 8 weeks on this induction diet. In contrast, no members of the prevention study groups of C57BL/6J mice, which had ADHF mixed into their diabetes induction diet, developed progressive evidence of NIDDM-like symptoms. Specific parameters observed to be significantly reduced in mice in the prevention diet groups fed ADHF mixed into the diabetes induction diet compared with the diabetes positive control groups fed diabetes induction diet only were clinical signs, blood glucose and serum insulin levels, insulin resistance and histopathological changes in major organs. This is the first report to our knowledge to show in vivo evidence for the successful prevention of progressive NIDDM-like symptoms by the addition of an anti-diabetes herbal formula to a effective diabetes induction diet.

Inhibition of the progression of type 2 diabetes in the C57BL/6J mouse model by an anti-diabetes herbal formula.

The effects of a specifically prepared anti-diabetic herbal formula (ADHF) on the course of established diet-induced type 2 diabetes in animal subjects has been studied. In a C57BL/6J mouse model for diet induced type 2 diabetes, intervention for 12 weeks using ADHF as a diet supplement resulted in a significant inhibition of diabetes related changes in major organs usually targeted by type 2 diabetes and a significant reduction in circulating levels of glucose and insulin. Young male mice were randomly assigned to receive ad libitum exposure to either a standard rodent chow diet or to a high fat, high simple sugar, low fibre diet (diabetes induction diet), respectively for 8 weeks. All mice fed the induction diet developed abnormally high blood glucose levels at 8 weeks. Animals with confirmed diet induced diabetic blood glucose levels were again randomly assigned into one of three groups (10 subjects per group), one group was thereafter fed only the diabetes induction diet and the other two groups were thereafter fed the diabetes induction diet into which ADHF had been mixed at 4% or at 8% fi nal concentrations. Normal mice were also randomized into two groups that were fed either a regular diet alone or 8% ADHF mixed in the regular diet. Blood glucose levels markedly increased over the 20 weeks of study in the diabetic mice fed the diabetes induction diet only. In contrast, diabetic mice fed induction diet into which 4% or 8% ADHF had been incorporated showed significantly decreased blood glucose and insulin levels over the time of the study. Additional parameters significantly reduced in diabetic mice fed ADHF included insulin resistance and histopathological changes in the pancreas and liver. This is the fi rst report to our knowledge to show in vivo evidence for significantly decreased circulating glucose and insulin levels and a significant reduction of progressive damage to major target organs by the addition of an herbal diet supplement to a diabetes induction diet proven to be capable of causing and maintaining type 2 diabetes.