Orchestration of ethylene and gibberellin signals determines primary root elongation in rice.

Primary root growth in cereal crops is fundamental for early establishment of the seedling and grain yield. In young rice (Oryza sativa) seedlings, the primary root grows rapidly for 7-10 days after germination and then stops; however, the underlying mechanism determining primary root growth is unclear. Here, we report that the interplay of ethylene and gibberellin (GA) controls the orchestrated development of the primary root in young rice seedlings. Our analyses advance the knowledge that primary root growth is maintained by higher ethylene production, which lowers bioactive GA contents. Further investigations unraveled that ethylene signaling transcription factor ETHYLENE INSENSITIVE3-LIKE 1 (OsEIL1) activates the expression of the GA metabolism genes GIBBERELLIN 2-OXIDASE 1 (OsGA2ox1), OsGA2ox2, OsGA2ox3, and OsGA2ox5, thereby deactivating GA activity, inhibiting cell proliferation in the root meristem, and ultimately gradually inhibiting primary root growth. Mutation in OsGA2ox3 weakened ethylene-induced GA inactivation and reduced the ethylene sensitivity of the root. Genetic analysis revealed that OsGA2ox3 functions downstream of OsEIL1. Taken together, we identify a molecular pathway impacted by ethylene during primary root elongation in rice and provide insight into the coordination of ethylene and GA signals during root development and seedling establishment.

Improved fluorescence-based assay for rapid screening and evaluation of SARS-CoV-2 main protease inhibitors.

The outbreak of coronavirus disease 2019 (COVID-19) pandemic caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global threat to human health. In parallel with vaccines, efficacious antivirals are urgently needed. SARS-CoV-2 main protease (Mpro) is an attractive drug target for antiviral development owing to its key roles in virus replication and host immune evasion. Due to the limitations of currently available methods, the development of novel high-throughput screening assays is of the highest importance for the discovery of Mpro inhibitors. In this study, we first developed an improved fluorescence-based assay for rapid screening of Mpro inhibitors from an anti-infection compound library using a versatile dimerization-dependent red fluorescent protein (ddRFP) biosensor. Utilizing this assay, we identified MG-101 as a competitive Mpro inhibitor in vitro. Moreover, our results revealed that ensitrelvir is a potent Mpro inhibitor, but baicalein, chloroquine, ebselen, echinatin, and silibinin are not. Therefore, this robust ddRFP assay provides a faithful avenue for rapid screening and evaluation of Mpro inhibitors to fight against COVID-19.

Abscisic acid promotes auxin biosynthesis to inhibit primary root elongation in rice.

Soil compaction is a global problem causing inadequate rooting and poor yield in crops. Accumulating evidence indicates that phytohormones coordinately regulate root growth via regulating specific growth processes in distinct tissues. However, how abscisic acid (ABA) signaling translates into auxin production to control root growth during adaptation to different soil environments is still unclear. In this study, we report that ABA has biphasic effects on primary root growth in rice (Oryza sativa) through an auxin biosynthesis-mediated process, causing suppression of root elongation and promotion of root swelling in response to soil compaction. We found that ABA treatment induced the expression of auxin biosynthesis genes and auxin accumulation in roots. Conversely, blocking auxin biosynthesis reduced ABA sensitivity in roots, showing longer and thinner primary roots with larger root meristem size and smaller root diameter. Further investigation revealed that the transcription factor basic region and leucine zipper 46 (OsbZIP46), involved in ABA signaling, can directly bind to the YUCCA8/rice ethylene-insensitive 7 (OsYUC8/REIN7) promoter to activate its expression, and genetic analysis revealed that OsYUC8/REIN7 is located downstream of OsbZIP46. Moreover, roots of mutants defective in ABA or auxin biosynthesis displayed the enhanced ability to penetrate compacted soil. Thus, our results disclose the mechanism in which ABA employs auxin as a downstream signal to modify root elongation and radial expansion, resulting in short and swollen roots impaired in their ability to penetrate compacted soil. These findings provide avenues for breeders to select crops resilient to soil compaction.

OsEIN2-OsEIL1/2 pathway negatively regulates chilling tolerance by attenuating OsICE1 function in rice.

Low temperature severely affects rice development and yield. Ethylene signal is essential for plant development and stress response. Here, we reported that the OsEIN2-OsEIL1/2 pathway reduced OsICE1-dependent chilling tolerance in rice. The overexpressing plants of OsEIN2, OsEIL1 and OsEIL2 exhibited severe stress symptoms with excessive reactive oxygen species (ROS) accumulation under chilling, while the mutants (osein2 and oseil1) and OsEIL2-RNA interference plants (OsEIL2-Ri) showed the enhanced chilling tolerance. We validated that OsEIL1 and OsEIL2 could form a heterxodimer and synergistically repressed OsICE1 expression by binding to its promoter. The expression of OsICE1 target genes, ROS scavenging- and photosynthesis-related genes were downregulated by OsEIN2 and OsEIL1/2, which were activated by OsICE1, suggesting that OsEIN2-OsEIL1/2 pathway might mediate ROS accumulation and photosynthetic capacity under chilling by attenuating OsICE1 function. Moreover, the association analysis of the seedling chilling tolerance with the haplotype showed that the lower expression of OsEIL1 and OsEIL2 caused by natural variation might confer chilling tolerance on rice seedlings. Finally, we generated OsEIL2-edited rice with an enhanced chilling tolerance. Taken together, our findings reveal a possible mechanism integrating OsEIN2-OsEIL1/2 pathway with OsICE1-dependent cascade in regulating chilling tolerance, providing a practical strategy for breeding chilling-tolerant rice.

Collaborative FFE-assisted simplified soft-output MLSE with LDPC for high-speed IM-DD transmissions.

In this paper, we propose a feed-forward equalizer (FFE)-assisted simplified soft-output MLSE (sMLSE) by collaborating the maximum likelihood sequence estimation (MLSE) with soft-decision low-density-parity-check (LDPC) decoding. The simplified sMLSE results in undetermined log-likelihood ratio (LLR) magnitudes when the reserved level is less than or equal to the half of modulation order. This severely degrades the performance of soft-decision forward error correction (SD-FEC) decoding. In the FFE-assisted simplified sMLSE, we use the LLRs calculated from pre-set FFE to replace these undetermined LLRs of simplified sMLSE. Thus, the proposed method eliminates the SD-FEC decoding performance degradation resulted from simplification. We conduct experiments to transmit 184-Gb/s PAM-4 or 255-Gb/s PAM-8 signal in IM-DD system at C-band to evaluate the performance of the proposed sMLSE. The results show that the proposed sMLSE can effectively compensate for the degradation of LLR quality. For 255-Gb/s PAM-8 signal transmissions, the FFE-assisted simplified sMLSE achieves almost the same SD-FEC decoding performance as the conventional sMLSE but with 85% complexity reduction.

Nondestructive measurement of terahertz optical thin films by machine learning based on physical consistency.

Optical scattering measurement is one of the most commonly used methods for non-contact online measurement of film properties in industrial film manufacturing. Terahertz photons have low energy and are non-ionizing when measuring objects, so combining these two methods can enable online nondestructive testing of thin films. In the visible light band, some materials are transparent, and their thickness and material properties cannot be measured. Therefore, a method based on physical consistency modeling and machine learning is proposed in this paper, which realizes the method of obtaining high-precision thin film parameters through single-frequency terahertz wave measurement, and shows good performance. Through the experimental measurement of organic material thin films, it is proved that the proposed method is an effective terahertz online detection technology with high precision and high throughput.

Three-Dimensional Multifrequency MR Elastography for Microvascular Invasion and Prognosis Assessment in Hepatocellular Carcinoma.

BACKGROUND: Pretreatment identification of microvascular invasion (MVI) in hepatocellular carcinoma (HCC) is important when selecting treatment strategies. PURPOSE: To improve models for predicting MVI and recurrence-free survival (RFS) by developing nomograms containing three-dimensional (3D) MR elastography (MRE). STUDY TYPE: Prospective. POPULATION: 188 patients with HCC, divided into a training cohort (n = 150) and a validation cohort (n = 38). In the training cohort, 106/150 patients completed a 2-year follow-up. FIELD STRENGTH/SEQUENCE: 1.5T 3D multifrequency MRE with a single-shot spin-echo echo planar imaging sequence, and 3.0T multiparametric MRI (mp-MRI), consisting of diffusion-weighted echo planar imaging, T2-weighted fast spin echo, in-phase out-of-phase T1-weighted fast spoiled gradient-recalled dual-echo and dynamic contrast-enhanced gradient echo sequences. ASSESSMENT: Multivariable analysis was used to identify the independent predictors for MVI and RFS. Nomograms were constructed for visualization. Models for predicting MVI and RFS were built using mp-MRI parameters and a combination of mp-MRI and 3D MRE predictors. STATISTICAL TESTS: Student's t-test, Mann-Whitney U test, chi-squared or Fisher's exact tests, multivariable analysis, area under the receiver operating characteristic curve (AUC), DeLong test, Kaplan-Meier analysis and log rank tests. P < 0.05 was considered significant. RESULTS: Tumor c and liver c were independent predictors of MVI and RFS, respectively. Adding tumor c significantly improved the diagnostic performance of mp-MRI (AUC increased from 0.70 to 0.87) for MVI detection. Of the 106 patients in the training cohort who completed the 2-year follow up, 34 experienced recurrence. RFS was shorter for patients with MVI-positive histology than MVI-negative histology (27.1 months vs. >40 months). The MVI predicted by the 3D MRE model yielded similar results (26.9 months vs. >40 months). The MVI and RFS nomograms of the histologic-MVI and model-predicted MVI-positive showed good predictive performance. DATA CONCLUSION: Biomechanical properties of 3D MRE were biomarkers for MVI and RFS. MVI and RFS nomograms were established. LEVEL OF EVIDENCE: 2 TECHNICAL EFFICACY: Stage 2.

Total neoadjuvant therapy for locally advanced rectal cancer: a three-group propensity score matched study.

PURPOSE: Total neoadjuvant therapy (TNT) has emerged as a therapeutic approach for locally advanced rectal cancer (LARC). However, the optimal chemotherapy cycles within TNT remain uncertain. This study aimed to evaluate and compare the prognostic efficacy of varying cycles of chemotherapy during TNT for LARC. METHODS: Patients diagnosed with LARC (T3-4N0M0/T1-4N1-2M0), who underwent TNT or chemoradiotherapy followed by total mesorectal excision (TME) between 2015 and 2020, were retrospective included. Patients were categorized into three groups based on their neoadjuvant strategy: CRT (long-course chemoradiotherapy), STNT (long-course CRT with one to three cycles of chemotherapy), and LTNT (long-course CRT with four or more cycles of chemotherapy). Propensity score matching (PSM) based on gender, age, body mass index, tumor distance from the anal verge, clinical T stage, clinical N stage, and mesorectal fascia status was employed to reduce confounding bias. Primary endpoints were disease-free survival (DFS) and metastasis-free survival (MFS). RESULTS: The study comprised 372 patients, with 73 patients in each group after PSM. Compared with CRT, both STNT and LTNT demonstrated improved DFS (5-year rate: 59.7% vs. 77.8% vs. 76.5%, p = 0.027) and MFS (5-year rate: 65.1% vs. 81.3% vs. 81.4%, p = 0.030). There was no difference in DFS or MFS between STNT and LTNT. These favorable outcomes were consistent among subgroups defined by tumor distance from the anal verge ≥ 5 cm, clinical T3 stage, clinical N positive status, or involved mesorectal fascia. CONCLUSION: Compared to CRT, both STNT and LTNT demonstrated improved DFS and MFS outcomes. Notably, survival outcomes were similar between STNT and LTNT, suggesting that chemotherapy cycles in TNT may not significantly impact survival.

Spatial Heterogeneity Directs Energy Dissipation in Condylar Fibrocartilage.

Condylar fibrocartilage with structural and compositional heterogeneity can efficiently orchestrate load-bearing and energy dissipation, making the temporomandibular joint (TMJ) survive high occlusion loads for a prolonged lifetime. How the thin condylar fibrocartilage can achieve efficient energy dissipation to cushion enormous stresses remains an open question in biology and tissue engineering. Here, three distinct zones in the condylar fibrocartilage are identified by analyzing the components and structure from the macro-and microscale to the nanoscale. Specific proteins are highly expressed in each zone related to its mechanics. The heterogeneity of condylar fibrocartilage can direct energy dissipation through the nano-micron-macro gradient spatial scale, by atomic force microscope (AFM), nanoindentation, dynamic mechanical analyzer assay (DMA), and the corresponding energy dissipation mechanisms are exclusive for each distinct zone. This study reveals the significance of the heterogeneity of condylar fibrocartilage in mechanical behavior and provides new insights into the research methods for cartilage biomechanics and the design of energy-dissipative materials.

SALT AND ABA RESPONSE ERF1 improves seed germination and salt tolerance by repressing ABA signaling in rice.

Rice (Oryza sativa) germination and seedling establishment, particularly in increasingly saline soils, are critical to ensure successful crop yields. Seed vigor, which determines germination and seedling growth, is a complex trait affected by exogenous (environmental) and endogenous (hormonal) factors. Here, we used genetic and biochemical analyses to uncover the role of an APETALA2-type transcription factor, SALT AND ABA RESPONSE ERF1 (OsSAE1), as a positive regulator of seed germination and salt tolerance in rice by repressing the expression of ABSCISIC ACID-INSENSITIVE5 (OsABI5). ossae1 knockout lines exhibited delayed seed germination, enhanced sensitivity to abscisic acid (ABA) during germination and in early seedling growth, and reduced seedling salt tolerance. OsSAE1 overexpression lines exhibited the converse phenotype, with increased seed germination and salt tolerance. In vivo and in vitro assays indicated that OsSAE1 binds directly to the promoter of OsABI5, a major downstream component of the ABA signaling pathway and acts as a major regulator of seed germination and stress response. Genetic analyses revealed that OsABI5-mediated ABA signaling functions downstream of OsSAE1. This study provides important insights into OsSAE1 regulation of seed vigor and salt tolerance and facilitates the practical use of OsSAE1 in breeding salt-tolerant varieties suitable for direct seeding cultivation.

On-chip all-optical second-order ordinary differential equation solver based on a single microdisk resonator.

This paper proposes an all-optical second-order ordinary differential equation (SODE) solver based on a single microdisk resonator. We validate the feasibility of our structure for constant and complex coefficient SODE solutions for Gaussian and super-Gaussian pulses. The results demonstrate a good agreement between the solutions obtained with the designed structure and those obtained through mathematical calculations for both constant and complex coefficient SODEs. We also discuss the influence of input optical signal pulse width on solution result deviations. Furthermore, we validate the capability of the designed structure to achieve tunable solutions for complex-coefficient SODEs with a tuning power of less than 10 mW. The device footprint is approximately 20×30 µm2, and it is 3-4 times smaller than the current smallest solving unit. The maximum Q-factor reaches 9.8×104. The proposed device avoids the traditional approach of cascading two resonators for SODE solving. Moreover, achieving mode alignment within the same resonator reduces the process challenges associated with aligning multiple devices in a cascade. Furthermore, it offers wider applicability for solving SODEs, namely, the ability to solve both constant and complex coefficient SODEs with complete derivative terms.

Burst errors suppression for MLSE in high-speed IM/DD transmission systems using PAM.

Maximum likelihood sequence estimation (MLSE) is the optimal signal sequence detection that can remove the inter-symbol interference (ISI). However, we find that the MLSE causes burst consecutive errors alternating between +2 and -2 in M-ary pulse amplitude modulation (PAM-M) IM/DD systems with large ISI. In this paper, we propose to use precoding to suppress the burst consecutive errors resulted from MLSE. A 2 M modulo operation is employed to guarantee that the probability distribution as well as the peak-to-average power ratio (PAPR) of encoded signal remain unchanged. After the receiver-side MLSE, the decoding process that involves adding the current MLSE output to the previous one and applying a 2 M modulo is implemented to break the burst consecutive errors. We conduct experiments to transmit 112/150-Gb/s PAM-4 or beyond 200-Gb/s PAM-8 signals at C-band to investigate the performance of the proposed MLSE integrated with precoding. The results show that the precoding can break burst errors effectively. For 201-Gb/s PAM-8 signal transmission, the precoding MLSE can achieve 1.4-dB receiver sensitivity gain and reduce the maximum length of burst consecutive errors from 16 to 3.

Total neoadjuvant therapy versus chemoradiotherapy for locally advanced rectal cancer: Bayesian network meta-analysis.

BACKGROUND: Total neoadjuvant therapy is a promising treatment for locally advanced rectal cancer, utilizing either short-course radiotherapy or long-course chemoradiotherapy, but their relative efficacy remains unclear. The aim of this Bayesian network meta-analysis was to investigate clinical outcomes amongst patients receiving total neoadjuvant therapy with short-course radiotherapy or long-course chemoradiotherapy, and those receiving long-course chemoradiotherapy alone. METHODS: A systematic literature search was performed. All studies that compared at least two of these three treatments for locally advanced rectal cancer were included. The primary endpoint was the pathological complete response rate, and survival outcomes were adopted as secondary outcomes. RESULTS: Thirty cohorts were included. Compared with long-course chemoradiotherapy, both total neoadjuvant therapy with long-course chemoradiotherapy (OR 1.78, 95 per cent c.i. 1.43 to 2.26) and total neoadjuvant therapy with short-course radiotherapy (OR 1.75, 95 per cent c.i. 1.23 to 2.50) improved the pathological complete response rate. Similar benefits were observed in the sensitivity and subgroup analyses, except for short-course radiotherapy with one to two cycles of chemotherapy. No significant differences in survival outcomes were found amongst the three treatments. Long-course chemoradiotherapy with consolidation chemotherapy (HR 0.44, 95 per cent c.i. 0.20 to 0.99) exhibited higher disease-free survival than long-course chemoradiotherapy alone. CONCLUSION: Compared with long-course chemoradiotherapy, both short-course radiotherapy with greater than or equal to three cycles of chemotherapy and total neoadjuvant therapy with long-course chemoradiotherapy can improve the pathological complete response rate, and long-course chemoradiotherapy with consolidation chemotherapy may lead to a marginal benefit in disease-free survival. The pathological complete response rate and survival outcomes are similar for total neoadjuvant therapy with short-course radiotherapy or long-course chemoradiotherapy.

An iterative BP-CNN decoder for optical fiber communication systems.

The conventional belief propagation (BP) of the low-density parity-check (LDPC) is designed based on additive white Gaussian noise (AWGN) close to the Shannon limit; however, the correlated noise due to chromatic dispersion or square-law detection results in a performance penalty in the intensity modulation and direct-detection (IM/DD) system. We propose an iterative BP cascaded convolution neural network (CNN) decoder to mitigate the correlated channel noise. We use a model of correlated Gaussian noise to verify that the noise correlation can be identified by the CNN and the decoding performance is improved by the iterative processing. We successfully demonstrate the proposed method in a 50-Gb/s 4-ary pulse amplitude modulation (PAM-4) IM/DD system. The simulation results show that the proposed decoder can achieve a BER performance improvement which is robust to transmission distance and launch optical power. The experimental results show that the iterative BP-CNN decoder outperforms the standard BP decoder by 1.2 dB in received optical power over 25-km SSMF.

Temporomandibular joint osteoarthritis: A review of animal models induced by surgical interventions.

OBJECTIVES: The pathological mechanism of temporomandibular joint osteoarthritis (TMJOA) is still unclear. Animal models induced by surgical interventions are one of the most ideal tools to imitate human pathological conditions. This review aims to define the similarities and differences of different surgical animal models. METHODS: Articles of TMJOA surgical animal models were collected including anterior disc displacement, disc perforation, and discectomy. We analyzed their experiments strategies based on comparing preoperative selection, intraoperative methodology, and postoperative manifestations. RESULTS: No matter which surgical intervention is selected, abnormal stress forces the whole joint to remodel its structure so that it could adapt to functional requirements, resulting in TMJOA eventually. However, anterior disc displacement needs more than 16 weeks to obtain typical manifestations, where the methodology is complicated. The course of perforation and discectomy is around 12-16 weeks, but they could cause excessive damage to the TMJ structure. CONCLUSIONS: All surgical interventions can cause TMJOA, but the extent of pathology varies from each other. This review will assist future experiments to better understand the pathogenesis of TMJOA and choose the most appropriate model.

Evaluation and prediction method of railway passenger long-term vibration comfort under complex operating conditions.

Vibration contributes large increases in railway passenger discomfort during long-term sitting. Discomfort caused by vibration may differ in different operation conditions. This paper conducted field measurements to investigate the interrelationships between the three. Participants completed a 240-min train journey with their whole-body vibration, subjective comfort ratings and train operating parameters being recorded. A large correlation was observed between the estimated vibration dose value and subjective comfort. The relationship that vibration magnitude significantly increases with increasing the train speed and tunnel density was also found and quantified. A vibration exposure limit of 2.08 m/s1.75 corresponding to the boundary between subjective ratings of comfortable and discomfortable was obtained. Based on the exposure limit and the quantified relationship, a vibration comfort prediction method that can calculate the passenger's maximum tolerance time under a given operation condition was proposed and may help in determining the optimal operating speed and tunnels distribution to alleviate vibration discomfort. Practitioner summary: Similar to the guide to effect of vibration on health in current standard, a vibration exposure limit regarding comfort was provided for reference when assessing long-term vibration comfort. Meanwhile, a prediction method was proposed for determining the best train operating speed and tunnels distribution, thereby alleviating railway passengers' vibration discomfort.

Advanced nonlinearity equalizer with TC-NL-MLSE for transmitting beyond 200-Gb/s PAM-8 in IM/DD systems.

The severe band-limited effect resulted from the low-cost optical transceiver increases the channel memory length and the number of taps of the equalizers. Besides, the interaction of fiber dispersion and square-law detection introduce nonlinear distortions in intensity modulation and direct-detection (IM/DD) transmission systems. The serious band-limited effect and nonlinear distortions degrade the transmission performance and bring challenges to current equalizers for low-complexity implementation. In this paper, we propose a trellis-compression nonlinear maximum likelihood sequence estimation (TC-NL-MLSE) algorithm to compensate the linear and nonlinear distortions with lower complexity. In the TC-NL-MLSE, we introduce a polynomial nonlinear filter (PNLF) to partly compensate both the linear distortions and nonlinear distortions. Then, we establish a look-up-table (LUT) to calculate the nonlinear branch metric (BM). To simplify the calculation, two or three levels with the highest probabilities are selected according to decision thresholds for each symbol to compress the state-trellis graph (STG). This significantly reduces computational complexity on BM calculations especially for high-order modulations. We conduct experiments to transmit beyond the 200-Gb/s PAM-8 signal over 2-km standard single mode fiber (SSMF) at C-band. The TC-NL-MLSE outperforms the reduced-state MLSE with PNLF, and can reach the 7%-overhead hard-decision forward error correction threshold. Moreover, the TC-NL-MLSE reduces the complexity by 97% compared with standard LUT-MLSE, limiting the multipliers around 100 at the expense of only 0.2-dB receiver sensitivity penalty.

Complexity curtailed MLSE equalizer based on a compact look-up-table for C-band DSB IM/DD transmission.

Chromatic dispersion appears to be a major performance limiting problem in optical intensity modulation direct detection (IM/DD) transmission systems, especially for a double-sideband (DSB) signal. We propose a complexity-reduced look-up-table based maximum likelihood sequence estimation (LUT-MLSE) for DSB C-band IM/DD transmission based on pre-decision-assisted trellis compression and a path-decision-assisted Viterbi algorithm. To further compress the size of the LUT and reduce the length of the training sequence, we proposed a finite impulse response (FIR) and LUT hybrid channel model for the LUT-MLSE. For PAM-6 and PAM-4, the proposed methods can compress the size of the LUT into 1/6 and 1/4, and reduce the number of multipliers by 98.1% and 86.6% with slight performance degradation. We successfully demonstrate a 20-km 100-Gb/s PAM-6 and a 30-km 80-Gb/s PAM-4 C-band transmission over dispersion-uncompensated links.

Variations in the TAS2R38 gene among college students in Hubei.

BACKGROUND: The bitter taste receptor gene TAS2R38 is a member of the human TAS2R gene family. Polymorphisms in TAS2R38 affect the ability to taste the bitterness of phenylthiourea (PTC) compounds, thus affecting an individual's food preference and health status. METHODS: We investigated polymorphisms in the TAS2R38 gene and the sensitivity to PTC bitterness among healthy Chinese college students in Hubei province. The association of TAS2R38 polymorphisms and PTC sensitivity with body mass index (BMI), food preference, and health status was also analyzed. A total of 320 healthy college students were enrolled (male: 133, female: 187; aged 18-23 years). The threshold value method was used to measure the perception of PTC bitterness, and a questionnaire was used to analyze dietary preferences and health status. Polymerase chain reaction (PCR) was used to analyze polymorphisms at three common TAS2R38 loci (rs713598, rs1726866, and rs10246939). RESULTS: In our study population, 65.00% of individuals had medium sensitivity to the bitterness of PTC; in contrast, 20.94% were highly sensitive to PTC bitterness, and 14.06% were not sensitive. For the TAS2R38 gene, the PAV/PAV and PAV/AAI diplotypes were the most common (42.19% and 40.63%, respectively), followed by the homozygous AVI/AVI (8.75%) and PAV/AVI (5.00%) diplotypes. CONCLUSION: There was a significant correlation between the sensitivity to PTC bitterness and sex, but there was no correlation between the common diplotypes of TAS2R38 and gender. Polymorphisms in the TAS2R38 gene were associated with the preference for tea, but not with one's native place, BMI, health status, or other dietary preferences. There was no significant correlation between the perception of PTC bitterness and one's native place, BMI, dietary preference, or health status. We hope to find out the relationship between PTC sensitivity and TAS2R38 gene polymorphisms and dietary preference and health status of Chinese population through this study, providing relevant guidance and suggestions for dietary guidance and prevention of some chronic diseases in Chinese population.

Assessment of passenger long-term vibration discomfort: a field study in high-speed train environments.

This study revealed the mechanism of long-term passenger vibration discomfort in high-speed trains and proposed a novel evaluation model to assess it, while the most popular international standard ISO 2631-1 is unsuitable. Here, a field test was conducted to investigate the long-term passenger vibration comfort in high-speed trains under different operation environments by the measurement of the whole-body vibration (WBV) and the subjective ratings of passenger comfort. During the whole sitting period of high-speed train passengers, the phenomena 'compensatory degradation' and 'discomfort accumulation' were found, which meant that the brief termination of vibration cannot fundamentally alleviate passenger vibration comfort. And the vibration comfort can be evaluated by the product of exposure time and the novel vibration acceleration index we proposed. Meanwhile, high-speed trains with higher velocities or running in tunnel environments have higher frequency-weighted WBV amplitude than open-air and lower velocities, which caused more vibration discomfort of passengers. Practitioner Summary: This field study provided data support for ensuring the occupational health of train drivers whose work routes involve a large number of tunnels and improving passenger vibration comfort. Meanwhile, a novel idea was provided for evaluating the vibration comfort of passengers who prolonged exposure to low-amplitude environments.