ABSTRACT
The search for novel anticancer drugs is essential to expand treatment options, overcome drug resistance, reduce toxicity, promote innovation, and tackle the economic impact. The importance of these studies lies in their contribution to advancing cancer research and enhancing patient outcomes in the battle against cancer. Here, we developed new asymmetric hybrids containing two different naphthoquinones linked by a 1,2,3-1H-triazole nucleus, which are potential new drugs for cancer treatment. The antitumor activity of the novel compounds was tested using the breast cancer cell lines MCF-7 and MDA-MB-231, using the non-cancer cell line MCF10A as control. Our results showed that two out of twenty-two substances tested presented potential antitumor activity against the breast cancer cell lines. These potential drugs, named here 12g and 12h were effective in reducing cell viability and promoting cell death of the tumor cell lines, exhibiting minimal effects on the control cell line. The mechanism of action of the novel drugs was assessed revealing that both drugs increased reactive oxygen species production with consequent activation of the AMPK pathway. Therefore, we concluded that 12g and 12h are novel AMPK activators presenting selective antitumor effects.
Subject(s)
Antineoplastic Agents , Breast Neoplasms , Naphthoquinones , Humans , Female , MCF-7 Cells , Reactive Oxygen Species/metabolism , Triazoles/pharmacology , Naphthoquinones/pharmacology , AMP-Activated Protein Kinases , Cell Proliferation , Apoptosis , Cell Line, Tumor , Antineoplastic Agents/pharmacology , Breast Neoplasms/drug therapy , Drug Screening Assays, AntitumorABSTRACT
The title compound, C14H11NO4, exists in the solid phase in the zwitterionic form, 2-{[(4-carboxy-3-hydroxyphenyl)iminiumyl]methyl}phenolate, with the H atom from the phenol group on the 2-hydroxybenzylidene ring transferred to the imine N atom, resulting in a strong intramolecular N-HâââO hydrogen bond between the iminium H atom and the phenolate O atom, forming a six-membered hydrogen-bonded ring. In addition, there is an intramolecular O-HâââO hydrogen bond between the carboxylic acid group and the adjacent hydroxy group of the other ring, and an intermolecular C-HâââO contact involving the phenol group and the C-H group adjacent to the imine bond, connecting the molecules into a two-dimensional network in the (103) plane. π-π stacking interactions result in a three-dimensional network. This study is important because it provides crystallographic evidence, supported by IR data, for the iminium zwitterionic form of Schiff bases.
ABSTRACT
This paper describes the preparation of N,N'-disubstituted ethylenediamine and imidazolidine derivatives and their in vitro biological activities against Leishmania species. Of the nine synthesized compounds, five displayed a good activity in both L. amazonensis and L. major promastigotes. The compounds 1,2-Bis(p-methoxybenzyl) ethylenediamine (4) and 1,3-Bis(p-methoxybenzyl)imidazolidines (5) showed the best activity on intracellular amastigotes, with IC50 values of 2.0 and 9.4 microgram/mL, respectively. In addition, none of compounds were cytotoxic against mammalian cells. The leishmanicidal activity can be related with inhibition of polyamine synthesis and cellular penetration within biological membranes.
Subject(s)
Antiprotozoal Agents/pharmacology , Ethylenediamines/pharmacology , Imidazolidines/pharmacology , Macrophages, Peritoneal/drug effects , Animals , Antiprotozoal Agents/chemical synthesis , Antiprotozoal Agents/chemistry , Cell Survival/drug effects , Cells, Cultured , Ethylenediamines/chemical synthesis , Ethylenediamines/chemistry , Imidazolidines/chemical synthesis , Imidazolidines/chemistry , Leishmania/drug effects , Leishmania major/drug effects , Macrophages, Peritoneal/cytology , Macrophages, Peritoneal/parasitology , Mice , Mice, Inbred BALB C , Models, Chemical , Molecular StructureABSTRACT
This paper describes the synthesis and in vitro biological activities of imidazolidine and hexahydropyrimidine derivatives against bacteria (Escherichia coli, Staphylococcus aureus and Mycobacterium tuberculosis) and Leishmania protozoa. Out of sixteen heterocyclic derivatives tested, none were cytotoxic against mammalian cells. The compounds showed significant bacterial effects and leishmanicidal activity. Compounds 4a and 4c were active against S. aureus and E. coli, respectively. Compounds 3a-3f, 4h and 4i presented promising results against M. tuberculosis, with MIC values ranging from 12.5 to 25.0 µg/mL, comparable to the "first and second line" drugs used to treat tuberculosis. Compounds 4a, 4c and 4e were active against L major. Three of them were structurally characterized by single-crystal X-ray diffraction.
Subject(s)
Anti-Bacterial Agents/chemical synthesis , Anti-Bacterial Agents/pharmacology , Antiparasitic Agents , Bacteria/drug effects , Imidazolidines , Leishmania/drug effects , Pyrimidines , Animals , Anti-Bacterial Agents/chemistry , Antiparasitic Agents/chemical synthesis , Antiparasitic Agents/chemistry , Antiparasitic Agents/pharmacology , Cells, Cultured , Crystallography, X-Ray , Humans , Imidazolidines/chemical synthesis , Imidazolidines/chemistry , Imidazolidines/pharmacology , Mice , Microbial Viability/drug effects , Models, Molecular , Molecular Structure , Mycobacterium tuberculosis/drug effects , Pyrimidines/chemical synthesis , Pyrimidines/chemistry , Pyrimidines/pharmacologyABSTRACT
The Schiff base N,N'-bis(salicylidene)-o-phenylenediamine (salophen) was prepared by the condensation of salicylaldehyde with o-phenylenediamine in ethanol solution. The compound was characterized by elemental analysis, infrared (IR), (1)H, (13)C and (1)H(15)N HMBC nuclear magnetic resonance (NMR) spectroscopic measurements, and also by X-ray diffraction. The tautomerism of salophen was also studied by calculations using density functional theory (DFT). Two of the three tautomers were shown to coexist. A comparison of the DFT data of the three tautomers has shown that the most stable one is salophen 1, which is in accordance with experimental X-ray crystallographic data.
Subject(s)
Salicylates/chemistry , Aldehydes/chemistry , Crystallography, X-Ray , Isomerism , Magnetic Resonance Spectroscopy , Models, Molecular , Quantum Theory , Schiff Bases/chemistry , Spectroscopy, Fourier Transform InfraredABSTRACT
We describe in this work the synthesis of nine new fluoroquinolone derivatives based on modifications at the C-7 position of the known fluoroquinolones cipro-, gati-, and moxifloxacin, as well as their antitubercular evaluation. The synthesis of these new analogues was improved using microwave irradiation, providing several advantages such as better yields and shorter reaction times, in comparison with classical reaction conditions. Derivatives 4, 5, and 7 exhibited promising antitubercular activities.