ABSTRACT
PGPR is an emulsifier (E476) widely used in the food industry. In this study, a gas chromatography-flame ionisation detection (GC-FID) method was developed for the quantitative characterisation of the polyglycerol composition of PGPR. The method was validated to analyse quantitatively the polyglycerol species in neat PGPR products and in PGPR samples present in a lipid matrix. This method consists of saponification, acidification and petroleum ether extraction to remove interfering fatty acids, neutralisation, silylation and finally GC-FID analysis. Phenyl ß-D-glucopyranoside was used as internal standard as sorbitol proved unsuitable due to its susceptibility to interference from Na/K chloride during silylation. The response factors of glycerol and diglycerol towards phenyl ß-D-glucopyranoside were determined using pure standards, while response factors of polyglycerols with a degree of polymerisation of at least 3 could be reliably estimated according to an effective carbon number (ECN) approach. The validity of the method applied to PGPR samples was further supported on the basis of a mass balance considering the experimentally determined polyglycerol and fatty acid content. Moreover, recoveries of di-, tri-, tetra- and pentaglycerol were more than 95% for various PGPR samples added to two different lipid matrices at 2 wt% and 5 wt% concentrations. Furthermore, the method proved to be very repeatable (with relative standard deviation values below 2.2%). On the other hand, the inevitable presence of glycerol in the lipid samples caused fouling of the detector and column overloading, requiring frequent cleaning of the detector and trimming off part of the column.
Subject(s)
Glycerol , Lipids , Polymers , Glycerol/analysis , Glycerol/analogs & derivatives , Polymers/chemistry , Chromatography, Gas , Lipids/analysis , Lipids/chemistry , Ricinoleic Acids/analysis , Ricinoleic Acids/chemistry , Flame IonizationABSTRACT
Traditional Beijing roast duck often suffers from uneven color and high sugar content after roasting. Water-in-oil (W/O) nanoemulsion is a promising alternative to replace high concentration of sugar solution used in sugaring process according to similarity-intermiscibility theory. Herein, 3% of xylose was embedded in the aqueous phase of W/O emulsion to replace 15% maltose solution. W/O emulsions with different ratios of lecithin (LEC) and polyglycerol polyricinoleate (PGPR) were constructed by high-speed homogenization and high-pressure homogenization. Distribution and penetration extent of solutions and emulsions through the duck skin, as well as the color uniformity of Beijing roast duck were analyzed. Emulsions with LEC:PGPR ratios of 1:3 and 2:2 had better stability. Stable interfacial film and spatial structure were important factors influencing emulsion stabilization. The stable W/O emulsions could more uniformly distribute onto the surface of duck skin and longitudinally penetrate through the skin than solutions.
Subject(s)
Ducks , Glycerol/analogs & derivatives , Lecithins , Ricinoleic Acids , Animals , Lecithins/chemistry , Emulsions/chemistry , Sugars , Water/chemistry , BeijingABSTRACT
In the process of validating the elevated zero maze, a common test of anxiety-like behavior, in our laboratory, we demonstrated an anxiolytic-like effect of castor oil and its primary component, ricinoleic acid. We tested the effects of vehicle and chlordiazepoxide in male mice in the elevated zero maze following a 30-min pretreatment time. Chlordiazepoxide is a United States Food and Drug Administration-approved drug that was previously shown to exert anxiolytic-like effects in both the elevated zero maze and elevated plus maze. Chlordiazepoxide was administered at doses of 5 or 10 mg/kg. We used 5% polyoxyl 35 castor oil (Kolliphor® EL) and saline as treatment vehicles and found that the effect of chlordiazepoxide on open zone occupancy and open zone entries was blunted when 5% Kolliphor was used as the vehicle. These tests demonstrated that chlordiazepoxide increased open zone occupancy and entries in the elevated zero maze more effectively when saline was used as the treatment vehicle and that Kolliphor dampened the anxiolytic-like effect of chlordiazepoxide when it was used as the treatment vehicle. Notably, 5% Kolliphor alone slightly increased baseline open zone occupancy and entries. Given that Kolliphor is a derivative of castor oil, we next tested the effect of 5% castor oil and 5% ricinoleic acid, which is a major component of castor oil. We found that both castor oil and ricinoleic acid increased open zone occupancy but not entries compared with saline. Altogether, our findings demonstrate that Kolliphor, castor oil, and ricinoleic acid may exert anxiolytic-like effects in male mice in the elevated zero maze. This potential anxiolytic-like effect of castor oil is consistent with its well-established beneficial effects, including anti-inflammatory, antioxidant, antifungal, and pain-relieving properties.
Subject(s)
Anti-Anxiety Agents , Anxiety , Castor Oil , Ricinoleic Acids , Animals , Ricinoleic Acids/pharmacology , Anti-Anxiety Agents/pharmacology , Male , Mice , Anxiety/drug therapy , Behavior, Animal/drug effects , Chlordiazepoxide/pharmacology , Maze Learning/drug effects , Exploratory Behavior/drug effectsABSTRACT
Abstract: INTRODUCTION: Dengue fever is a viral disease transmitted by the Aedes aegypti Linn. (1792) (Diptera: Culicidae) mosquito, which is endemic in several regions of Brazil. Alternative methods for the control of the vector include botanical insecticides, which offer advantages such as lower environmental contamination levels and less likelihood of resistant populations. Thus, in this study, the ability of botanical insecticide formulations to inhibit the activity of the liver enzymes serum cholinesterase and malate dehydrogenase was evaluated. METHODS: Inhibition profiles were assessed using in vitro assays for cholinesterase and malate dehydrogenase activity and quantitated by ultraviolet-visible spectroscopy at 410nm to 340nm. RESULTS Insecticide products formulated from cashew nutshell liquid [A] and ricinoleic acid [B] showed cholinesterase activity levels of 6.26IU/mL and 6.61IU/mL, respectively, while the control level for cholinesterase was 5-12IU/mL. The products did not affect the level of 0.44IU/mL established for malate dehydrogenase, as the levels produced by [A] and [B] were 0.43IU/mL and 0.45IU/mL, respectively. CONCLUSIONS Our findings show that in vitro testing of the formulated products at concentrations lethal to A. aegypti did not affect the activity of cholinesterase and malate dehydrogenase, indicating the safety of these products.
Subject(s)
Humans , Animals , Ricinoleic Acids/pharmacology , Cholinesterase Inhibitors/pharmacology , Cholinesterases/drug effects , Anacardium/chemistry , Insecticides/pharmacology , Liver/enzymology , Malate Dehydrogenase/antagonists & inhibitors , Spectrophotometry, Ultraviolet , In Vitro Techniques , Ricinoleic Acids/isolation & purification , Aedes , Insect Vectors/drug effects , Insecticides/isolation & purificationABSTRACT
Este estudo analisou o comportamento do polímero poliuretano da mamona implantado em leito subperióstico em ângulos mandibulares de ratos. Vinte Rattus novergicus (var.Albinus rodentia mammalia, cepa Wispar) foram divididos em quatro grupos de cinco animais. O polímero poliuretano da mamona, com adiçäo de carbonato de cálcio, foi implantado no bordo inferior da mandíbula dos mesmos. Em cada grupo, um animal correspondeu ao controle, tendo sido submetido à cirurgia sem a colocaçäo do implante. Os animais foram sacrificados após 15, 30, 60 e 90 dias pós-operatórios. Ao exame histológico, em cortes corados com hematoxilina e eosina (HE), observou-se a estabilidade do implante, a ausência significativa de reaçäo inflamatória, a formaçäo de cápsula fibrosa circunjacente ao implante, e neoformaçäo óssea. Segundo as condiçöes experimentais, concluímos que o polímero poliuretano da mamona, implantado em leito subperióstico, näo desencadeou reaçäo inflamatória tardia significativa, tendo levado ao desenvolvimento de cápsula fibrosa circunscrita, com evoluçäo para neoformaçäo óssea. Sinais de rejeiçäo, exposiçäo ou expulsäo do implante näo foram observados