Immunostimulatory effects of Nannochloropsis oculata supplementation on Barki rams growth performance, antioxidant assay, and immunological status.

BACKGROUND: Natural feed supplements are gaining popularity in the animal production sector due to their safety and potential immunostimulatory properties, as well as the ban of some antibiotics and their negative residual effects. This study was carried out for 1 month to investigate the effect of Nannochloropsis oculata supplementation on growth performance and cell-mediated immunological status of rams assessed by leukogram assessment, lipid oxidation product malondialdehyde (MDA), total antioxidant capacity (TAC), interleukin assay after lymphocyte transformation test (LTT) including interleukin 6 (IL6), tumor necrosis factor-alpha (TNF-α), interleukin 12 (IL12), and gamma interferon (γ-IF), as well as Comet assay (% of DNA damage, tail length (px), % DNA in tail, tail moment and Olive tail moment). METHODS: Eighteen Barki rams (26.21 ± 0.64 kg) were divided into 3 equal treatment groups (6 sheep/each), G1: animals served as the control group that was fed the basal diet only, while the other treated groups (G2 and G3 (Nan 1.5% and Nan 3%) were fed the basal diet supplemented with 1.5% and 3% N. oculata (dry matter basis), respectively. RESULTS: The obtained results revealed that G3 showed a significant (P < 0.05) improvement in performance (body weight and body weight gain), the highest significant count (P < 0.05) in lymphocytes, and the lowest significant (P < 0.05) levels of neutrophils and neutrophils and lymphocytes ratio (N/L) ratio. Meanwhile, both levels of N. oculata significantly (P < 0.05) decreased MDA and increased TAC than control which seemed to be directly correlated with supplemented dose. There was a significant (P < 0.05) enhancement in the lymphocyte transformation assay produced significant (P < 0.05) high cytokines (IL6, γ-IF, IL12, and TNF-α) and the lowest significant (P <0.05) percent of DNA damage. The conducted principal component analysis estimated the inter-relationship between parameters and revealed that microalgae correlated strongly with cytokine assay and TAC, and negatively with Comet assay parameters; MDA, and neutrophils. CONCLUSIONS: It can be noted that dietary addition of N. oculata 3% increased sheep's performance while also producing significant-high cytokines. It also enhanced sheep immunology by considerably enhancing lymphocyte transformation ability. The antioxidant activity of Nannochloropsis appears to influence these findings. It was proposed that the Barki rams' basal diet be supplemented with 3% N. oculata.

Impacts of dietary supplementation with nano-iron and methionine on growth, blood chemistry, liver biomarkers, and tissue histology of heat-stressed broiler chickens.

A 28-day study was done to explore the impact of nano-iron alone or combined with methionine on growth, blood chemistry, liver biomarkers, and tissue histology of heat-stressed chicken. One-day-old Ross 308 chicks were randomly allocated to three groups. Each group was divided into three replicates (13 chicks/replicate). The first group was the control one that was fed a basal diet without supplementation (T0). The second group was fed a basal diet with nano-iron 4 mg kg-1 diet (T1). The third group was fed a basal diet with nano-iron 4 mg kg-1 diet plus methionine 4 g kg-1 diet (T2). The results showed that the birds in the control group had significantly (p < 0.05) higher final weights. Also, a partial relief of heat stress adverse effects was observed on growth by T1 compared to T2. The T2 showed a significantly increased (p < 0.05) free iron (Fe) level and transferrin saturation index. Likewise, T2 significantly (p < 0.05) reduced total iron-binding capacity (TIBC) and transferrin level in comparison with T0 and T1. Also, hepatic impairment and inflammatory response were observed in the T2 group when compared to T0 and T1, besides a bad lipid profile. Further, T2 showed raised levels of Fe and ferritin in their hepatic tissues compared to those T1 and T0. A significant increment of thiobarbituric acid reactive and decrement of reduced glutathione levels in the hepatic tissues of T2 and T1 versus T0 levels were recorded. It is concluded that nano-iron at the level of 4 mg kg-1 in this study is highly absorbed, leading to harmful effects. Further investigations are needed to detect the proper supplemental level.

Assessment of modulatory activity of Uncaria tomentosa extract against fipronil immunotoxicity in male rats.

This study has investigated the effect of using the Uncaria tomentosa (UT) extract against immunotoxicity that induced by fipronil (FP) in male Wistar rats. Twenty-eight, male Wistar rats were assigned randomly into four groups (7 rats each). Control group received distilled water. FP group received FP 9.7 mg/kg b. wt orally via gastric tube. UT group received 120 mg/kg b. wt. of UT extract orally. FP-UT group received both FP and UT (9.7 and 120 mg/kg b.wt, respectively) for 30 days. Hematological parameters, malondialdehyde (MDA), total antioxidant capacity (TAC), estradiol, histamine and immunoglobulin E (IGE) were assayed. Histopathological and electron microscopical examinations were performed to the lymphoid organs. Hematological parameters, were decreased in the FP group than the control group. There was a rise in MDA of FP group followed by a decrease in TAC content with histological and ultrastructure degenerative changes. UT extract treatment ameliorated the FP-induced perturbations for the former parameters. The results showed that FP treatment exerted an immunotoxic effect through acting as an endocrine disruptor and allergic, pro-inflammatory that was confirmed by histopathological and ultrastructure study of the lymphoid organs. Uncaria tomentosa extract could successfully modulate FP-induced immunotoxicity by diminishing all the studied parameters.

Comparative immunomodulatory efficacy of rosemary and fenugreek against Escherichia coli infection via suppression of inflammation and oxidative stress in broilers.

Broiler chickens are frequently infected with Escherichia coli (E. coli) bacteria, which often leads to the emergence of many diseases and high economic losses. Hence, the current study was conducted to assess the relative efficacy of dietary rosemary and fenugreek, under E. coli infection in broilers and their ability to replace antimicrobials without any loss of productivity or negative influence on broiler health, via evaluation of growth performance, biochemical indices, immune response and histo-morphological changes. Eighty Cobb broilers were allotted to four equal groups (n = 20 chicks/group): control non-infected (CN), control infected (CI), rosemary infected (RI) and fenugreek infected (FI) groups. The RI and FI groups revealed a significant elevation in their body weight and body weight gain compared with the CI group. However, both groups showed a significant decline in serum aspartate and alanine aminotransferase activities, as well as uric acid and creatinine levels. A significant decrease in total antioxidant capacity, catalase and superoxide dismutase activities was noted among CI chicks. Moreover, distinctly higher activities were evident in both RI and FI groups. Assessment of immunomodulatory markers showed a significant increase in immunoglobulin G along with a significant decline in interleukin-6 level in both RI and FI groups, with the lowest IL-6 value within FI group. Histopathological evaluations focused on the deleterious effect associated with E. coli infection of broilers' liver, kidney, intestine, spleen, bursa of Fabricius and thymus. Partial histological improvement was noticed among RI group, and nearly normal tissues were recorded in FI group. Overall, the obtained findings suggest the ability of fenugreek to mitigate the adverse effects of E. coli infection on broiler performance and tissue profiles, by improving the general health status of the broiler chickens.

Impact of garlic (Allium sativum) oil on cisplatin-induced hepatorenal biochemical and histopathological alterations in rats.

Cisplatin (cis-diamminedichloridoplatinum II [CDDP]) is a chemotherapeutic agent used for treating different cancers types. However, its usage is limited because it induces harmful toxicities in multiple organs, including nephrotoxicity and hepatotoxicity. Garlic oil (GO) has several pharmacological activities, including antioxidant activity. The aim of the study is to evaluate the protective and antioxidant effects of GO against CDDP-induced acute liver and kidney injuries in male rats. CDDP-treated rats showed increased serum ALT, AST, ALP, LDH, uric acid, urea, creatinine, and IL-6 levels. Moreover, CDDP-treated rats showed significantly increased MDA and NO levels and decreased GSH level and T.SOD and CAT activities in hepatic and renal tissues compared with control rats. GO administration, especially at a dose of 100 ml/kg, alleviated CDDP-induced adverse biochemical and histopathological alterations and restored them to their normal values. These results suggest that GO reverses CDDP-induced hepatorenal damage by exerting antioxidant and anti-inflammatory effects.

Hepatorenal protective effects of taurine and N-acetylcysteine against fipronil-induced injuries: The antioxidant status and apoptotic markers expression in rats.

Fipronil (FPN), a commonly used phenylpyrazole pesticide can induce oxidative tissue damage following hazard usage. Due to the extensive household and commercial usage of FPN, its toxic effects on mammals received considerable attention. Finding the proper antioxidant that can overcome FPN-induced damage is essential. Therefore, the present study aimed to assess the hepatorenal ameliorative outcomes of N-acetyl cysteine (NAC) and taurine (TAU) against hepatorenal damage induced by FPN in male Wistar rats. Compared to control rats, oral FPN (at a dose of 19.4 mg kg-1 BW for five successive days) significantly increased serum activities (p ≤ 0.05) of alkaline phosphatase, lactate dehydrogenase and transaminases, in addition to total cholesterol, urea and creatinine levels. Moreover, FPN provoked oxidative damage indicated by increased malondialdehyde and nitric oxide formation and decreased glutathione concentration and activities of enzymatic antioxidants (superoxide dismutase, glutathione peroxidase and catalase) in the hepatic and renal tissues. Furthermore, FPN administration induced overexpression of the proapoptotic (Bax), while it downregulated the expression of the anti-apoptotic (Bcl-2) protein. Interestingly, oral administration of TAU (50 mg Kg-1 BW) and NAC (50 mg Kg-1 BW), alone or in combination, five days prior to and five days along with FPN administration, significantly ameliorated (p ≤ 0.05) and normalized the harmful effects of FPN on serum biomarkers of hepatorenal injury, lipid peroxidation and tissue antioxidants. In conclusion, TAU and NAC, alone or in combination, provided significant hepatorenal protection against oxidative stress and apoptosis induced by FPN.

Lycopene: Hepatoprotective and Antioxidant Effects toward Bisphenol A-Induced Toxicity in Female Wistar Rats.

Bisphenol A (BPA)-an endocrine disruptor xenoestrogen-is widely spread in the environment. Lycopene (LYC) is an antioxidant phytochemical carotenoid. The hereby study was designed to verify the deleterious effect of BPA on cyclic female rats' hepatic tissue as well as evaluation of the effect of LYC toward BPA hepatic perturbation. Twenty-eight female Wistar rats were allocated equally into four groups: control group, LYC group (10 mg/kg B.wt), BPA group (10 mg/kg B.wt), and BPA + LYC group (the same doses as former groups). The treatments were given daily via gavage to the rats for 30 days. The rats in BPA displayed high activities of serum liver enzymes with low levels of total proteins (TP) and albumin. Moreover, BPA induced hepatic oxidative stress via depletion of antioxidant enzymes concomitant with augmentation of lipid peroxidation, increased comet tail DNA %, and overexpression of caspase-3. Meanwhile, LYC administration reduced the cytotoxic effects of BPA on hepatic tissue, through improving the liver function biomarkers and oxidant-antioxidant state as well as DNA damage around the control values. These findings were confirmed by hepatic histopathological examination. Finally, LYC credited to have a noticeable protective effect versus BPA provoked oxidative injury and apoptosis of the liver tissue.