Analyses of oxidative DNA damage among coal vendors via single cell gel electrophoresis and quantification of 8-hydroxy-2'-deoxyguanosine.

Looking at the development status of Nigeria and other developing nations, most low-income and rural households often use coal as a source of energy which necessitates its trade very close to the communities. Moreover, the effects of exposure to coal mining activities are rarely explored or yet to be studied, not to mention the numerous street coal vendors in Nigeria. This study investigated the oxidative stress levels in serum and urine through the biomarker 8-OHdG and DNA damage via single cell gel electrophoresis (alkaline comet assay). Blood and urine levels of 8-OHdG from 130 coal vendors and 130 population-based controls were determined by ELISA. Alkaline comet assay was also performed on white blood cells for DNA damage. The average values of 8-OHdG in serum and urine of coal vendors were 22.82 and 16.03 ng/ml respectively, which were significantly greater than those detected in controls (p < 0.001; 15.46 and 10.40 ng/ml of 8-OHdG in serum and urine respectively). The average tail length, % DNA in tail and olive tail moment were 25.06 µm, 18.71% and 4.42 respectively for coal vendors. However, for controls, the average values were 4.72 µm, 3.63% and 1.50 for tail length, % DNA in tail and olive tail moment respectively which were much lower than coal vendors (p < 0.001). Therefore, prolonged exposure to coal dusts could lead to higher serum and urinary 8-OHdG and significant DNA damage in coal vendors observed in tail length, % DNA in tail, and olive tail moment by single cell gel electrophoresis. It is therefore established that coal vendors exhibit a huge risk from oxidative stress and assessment of 8-OHdG with single cell gel electrophoresis has proven to be a feasible tool as biomarkers of DNA damage.

Deep learning ResNet34 model-assisted diagnosis of sickle cell disease via microcolumn isoelectric focusing.

Traditional methods for sickle cell disease (SCD) screening can be inaccurate and misleading, and the early and accurate diagnosis of SCD is crucial for effective management and treatment. Although microcolumn isoelectric focusing (mIEF) is effective, the hemoglobinopathies must be accurately identified, wherein skilled personnel are required to analyse the bands in mIEF. Further automating and standardizing the diagnostic methods via AI to identify abnormal Hbs would be a useful endeavor. In this study, we propose a novel approach for SCD diagnosis by integrating the high throughput capability of ResNet34 in image analysis, as a deep learning convolutional neural network, for the precise separation of Hb variants using mIEF. Initially, SCD blood samples were subjected to mIEF and the resulting patterns were then captured as digital images. The sensitivity and specificity of the mIEF analysis were 100% and 97.8%, respectively, with a 99.39% accuracy. Comparison with HPLC showed a strong linear correlation (R2 = 0.9934), good agreement with the Bland-Altman plot (average difference ± 1.96 SD, bias = 9.89%) and a 100% match with the DNA analysis. Subsequently, the mIEF images were then input into the ResNet34 model, pre-trained on a large dataset, for feature extraction and classification. The integration of ResNet34 with mIEF demonstrated promising results in terms of precision (90.1%) and accuracy in distinguishing between the various SCD conditions. Overall, the proposed method offers a more effective, automated, and reduced cost approach for SCD diagnosis, which could potentially streamline diagnostic workflows and mitigate the subjectivity and variability inherent in manual assessments.

Welding fumes composition and their effects on blood heavy metals in albino rats.

Toxic substances produced during welding include heavy metals, carbon monoxide, carbon dioxide, and nitrogen oxides. The study aims to evaluate the heavy metals concentration in welding fumes and the blood of the animals exposed to welding fumes. The fumes were collected from a welding site by a skilled welder and part of it was subjected to metals analysis. A total of 130 rats were divided into 13 groups. 12 groups were given doses calculated to correspond to real-life workers exposure regimes and 1 group served as control. The dosages were administered intratracheally after anesthetization weekly for 12 weeks. The animals were sacrificed and whole blood samples were collected for atomic absorption spectrophotometry. The metals in fumes analyzed were decreasing in order of Fe > K > Pb > Co > Cd > Ca > Ni > Mn > Zn > Cr > Al > Cu > Mg. Changes were observed in the behaviour of the test animals compared to the control indicating probable toxicity. The values of Pb, Cr, Fe, Mn, and Ni in the exposed animal's blood were higher than the control and increased relatively across the treatment groups. However, the values of Al and Zn were not significantly different from the control. These indicate that exposure to welding fumes having contained a significant amount of heavy metals has caused noticeable toxicity symptoms with simultaneous elevation in blood metal levels. Monitoring and regulation of these activities should be enforced by relevant authorities in Kano and Nigeria in general.

Toxicity effects of Kano central abattoir effluent on Clarias gariepinus juveniles.

The contamination of water from rivers or land by effluent of abattoir could cause a pronounced health and environmental hazard. The present study was aimed at determining the acute effects abattoir effluents on Clarias gariepinus juveniles. It involved the determination of physicochemical parameters of the water and the hematological parameters of Clarias gariepinus juveniles. In addition, histopathological features of gills, kidney and liver were assessed. From the study, it was observed that abattoir effluent does not cause a significant change in temperature of the water but reduction in pH and DO values across the groups. Thus, it has induced a remarkable effects on the hematological parameters by causing a significant elevation in MCV, PLT and MCH and reduction in WBC count, RBC count, HGB, LYM and MPV (p < 0.05) than the control. These have led to pronounced changes in the pathologies of gills and liver which include degenerative changes in the oedema and secondary lamellae, cytoplasmic vacuolation of the hepatic tissue respectively. However, the renal tissues were unaffected. It is therefore be concluded that, abbatoir effluent posses some toxicological properties which have been observed in blood, gills and liver tissues of Clarias gariepinus juveniles. Government and other stakeholders should monitor and regulate discharge of the effluent into nearby water bodies.

Evaluation of some heavy metals concentration in body fluids of metal workers in Kano metropolis, Nigeria.

Metal workers in urban Kano constitute a major workforce with a considerable population. The present work was aimed at obtaining baseline data on the extent of metal ion concentration in body fluids (urine and blood) of sampled population in the area. The investigation involves interaction with sampled population as well as blood and urine sample collection for heavy metals analysis. The health problems associated with the practice identified by respondents include: metal fume fever; eye and skin irritation; dizziness and respiratory problems; lack of or inadequate protective devices during activity were also reported. Laboratory investigation of urine samples by Atomic absorption spectrophotometry indicated higher concentrations for Manganese (Mn), Lead (Pb) and Nickel (Ni); in blood samples, there were higher concentrations of Manganese (Mn), Lead (Pb), Chromium (Cr) and Nickel (Ni). Metal workers of urban Kano are at risk because of the concentration of Mn and Pb in particular. There is the need to monitor occupational activities that are responsible for pollution and with serious health risk.

A Bio-assessment of DNA damage by Alkaline Comet Assay in metal workers of Kano metropolis, Nigeria.

Metallic work is one of the widespread economic activities in urban Kano. Little or no attention is usually directed at occupational health risk by local or state authorities in Kano. The present work was aimed at the evaluation of DNA damage in metal workers by Alkaline Comet Assay in blood lymphocytes. The results showed that there was significant difference statistically between the level of DNA damage in blood lymphocytes of metal workers and control group (p < 0.05). In addition, the level of damage to DNA in blood of subjects with long term exposure and old age is of serious concern. There is the need to monitor occupational activities that can pose serious health risks. The relative ignorance of the metal workers about the health risks they are exposed to as well as the public should be addressed.