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1.
FASEB J ; 35(5): e21552, 2021 05.
Artículo en Inglés | MEDLINE | ID: mdl-33826788

RESUMEN

During episodes of acute inflammation, polymorphonuclear leukocytes (PMNs) are actively recruited to sites of inflammation or injury where they provide anti-microbial and wound-healing functions. One enzyme crucial for fulfilling these functions is myeloperoxidase (MPO), which generates hypochlorous acid from Cl- and hydrogen peroxide. The potential exists, however, that uncontrolled the extracellular generation of hypochlorous acid by MPO can cause bystander tissue damage and inhibit the healing response. Previous work suggests that the microbiota-derived tryptophan metabolites 1H-indole and related molecules ("indoles") are protective during intestinal inflammation, although their precise mechanism of action is unclear. In the present work, we serendipitously discovered that indoles are potent and selective inhibitors of MPO. Using both primary human PMNs and recombinant human MPO in a cell-free system, we revealed that indoles inhibit MPO at physiologic concentrations. Particularly, indoles block the chlorinating activity of MPO, a reliable marker for MPO-associated tissue damage, as measured by coulometric-coupled HPLC. Further, we observed direct interaction between indoles and MPO using the established biochemical techniques microscale thermophoresis and STD-NMR. Utilizing a murine colitis model, we demonstrate that indoles inhibit bystander tissue damage, reflected in decreased colon 3-chlorotyrosine and pro-inflammatory chemokine expression in vivo. Taken together, these results identify microbiota-derived indoles that acts as endogenous immunomodulatory compounds through their actions on MPO, suggesting a symbiotic association between the gut microbiota and host innate immune system. Such findings offer exciting new targets for future pharmacological intervention.


Asunto(s)
Adenocarcinoma/patología , Efecto Espectador , Colitis/patología , Neoplasias Colorrectales/patología , Indoles/farmacología , Neutrófilos/enzimología , Peroxidasa/antagonistas & inhibidores , Adenocarcinoma/inmunología , Adenocarcinoma/metabolismo , Animales , Colitis/inmunología , Colitis/metabolismo , Neoplasias Colorrectales/inmunología , Neoplasias Colorrectales/metabolismo , Halogenación , Humanos , Ratones , Ratones Endogámicos C57BL , Microbiota , Células Tumorales Cultivadas , Tirosina/metabolismo
2.
J Biol Chem ; 293(16): 6039-6051, 2018 04 20.
Artículo en Inglés | MEDLINE | ID: mdl-29487135

RESUMEN

Intestinal epithelial cells form a selectively permeable barrier to protect colon tissues from luminal microbiota and antigens and to mediate nutrient, fluid, and waste flux in the intestinal tract. Dysregulation of the epithelial cell barrier coincides with profound shifts in metabolic energy, especially in the colon, which exists in an energetically depleting state of physiological hypoxia. However, studies that systematically examine energy flux and adenylate metabolism during intestinal epithelial barrier development and restoration after disruption are lacking. Here, to delineate barrier-related energy flux, we developed an HPLC-based profiling method to track changes in energy flux and adenylate metabolites during barrier development and restoration. Cultured epithelia exhibited pooling of phosphocreatine and maintained ATP during barrier development. EDTA-induced epithelial barrier disruption revealed that hypoxanthine levels correlated with barrier resistance. Further studies uncovered that hypoxanthine supplementation improves barrier function and wound healing and that hypoxanthine appears to do so by increasing intracellular ATP, which improved cytoskeletal G- to F-actin polymerization. Hypoxanthine supplementation increased the adenylate energy charge in the murine colon, indicating potential to regulate adenylate energy charge-mediated metabolism in intestinal epithelial cells. Moreover, experiments in a murine colitis model disclosed that hypoxanthine loss during active inflammation correlates with markers of disease severity. In summary, our results indicate that hypoxanthine modulates energy metabolism in intestinal epithelial cells and is critical for intestinal barrier function.


Asunto(s)
Colitis/metabolismo , Colon/metabolismo , Metabolismo Energético , Hipoxantina/metabolismo , Mucosa Intestinal/metabolismo , Animales , Colitis/patología , Colon/patología , Femenino , Mucosa Intestinal/patología , Metaboloma , Ratones , Ratones Endogámicos C57BL , Consumo de Oxígeno , Permeabilidad , Uniones Estrechas/metabolismo , Uniones Estrechas/patología
3.
Am J Pathol ; 188(5): 1183-1194, 2018 05.
Artículo en Inglés | MEDLINE | ID: mdl-29454749

RESUMEN

Interactions between the gut microbiota and the host are important for health, where dysbiosis has emerged as a likely component of mucosal disease. The specific constituents of the microbiota that contribute to mucosal disease are not well defined. The authors sought to define microbial components that regulate homeostasis within the intestinal mucosa. Using an unbiased, metabolomic profiling approach, a selective depletion of indole and indole-derived metabolites was identified in murine and human colitis. Indole-3-propionic acid (IPA) was selectively diminished in circulating serum from human subjects with active colitis, and IPA served as a biomarker of disease remission. Administration of indole metabolites showed prominent induction of IL-10R1 on cultured intestinal epithelia that was explained by activation of the aryl hydrocarbon receptor. Colonization of germ-free mice with wild-type Escherichia coli, but not E. coli mutants unable to generate indole, induced colonic epithelial IL-10R1. Moreover, oral administration of IPA significantly ameliorated disease in a chemically induced murine colitis model. This work defines a novel role of indole metabolites in anti-inflammatory pathways mediated by epithelial IL-10 signaling and identifies possible avenues for utilizing indoles as novel therapeutics in mucosal disease.


Asunto(s)
Colitis/metabolismo , Indoles/metabolismo , Mucosa Intestinal/metabolismo , Microbiota/fisiología , Receptores de Interleucina-10/metabolismo , Animales , Línea Celular , Colitis/inducido químicamente , Sulfato de Dextran , Modelos Animales de Enfermedad , Homeostasis/fisiología , Humanos , Metabolómica , Ratones
4.
J Immunol ; 199(8): 2976-2984, 2017 10 15.
Artículo en Inglés | MEDLINE | ID: mdl-28893958

RESUMEN

Commensal interactions between the enteric microbiota and distal intestine play important roles in regulating human health. Short-chain fatty acids (SCFAs), such as butyrate, produced through anaerobic microbial metabolism represent a major energy source for the host colonic epithelium and enhance epithelial barrier function through unclear mechanisms. Separate studies revealed that the epithelial anti-inflammatory IL-10 receptor α subunit (IL-10RA) is also important for barrier formation. Based on these findings, we examined if SCFAs promote epithelial barrier through IL-10RA-dependent mechanisms. Using human intestinal epithelial cells (IECs), we discovered that SCFAs, particularly butyrate, enhanced IEC barrier formation, induced IL-10RA mRNA, IL-10RA protein, and transactivation through activated Stat3 and HDAC inhibition. Loss and gain of IL-10RA expression directly correlates with IEC barrier formation and butyrate represses permeability-promoting claudin-2 tight-junction protein expression through an IL-10RA-dependent mechanism. Our findings provide a novel mechanism by which microbial-derived butyrate promotes barrier through IL-10RA-dependent repression of claudin-2.


Asunto(s)
Bacterias Anaerobias/fisiología , Butiratos/metabolismo , Colon/patología , Microbioma Gastrointestinal/inmunología , Mucosa Intestinal/fisiología , Receptores de Interleucina-10/metabolismo , Uniones Estrechas/metabolismo , Butiratos/inmunología , Línea Celular , Células Cultivadas , Claudina-2/metabolismo , Regulación de la Expresión Génica , Histona Desacetilasas/metabolismo , Humanos , Mucosa Intestinal/microbiología , Mucosa Intestinal/patología , Receptores de Interleucina-10/genética , Factor de Transcripción STAT3/genética , Factor de Transcripción STAT3/metabolismo , Simbiosis , Activación Transcripcional , Migración Transendotelial y Transepitelial , Regulación hacia Arriba
5.
BMC Physiol ; 15: 3, 2015 Jun 05.
Artículo en Inglés | MEDLINE | ID: mdl-26040642

RESUMEN

BACKGROUND: There is increasing evidence that poor growth of preterm infants is a risk factor for poor long-term development, while the effects of early postnatal growth restriction are not well known. We utilized a rat model to examine the consequences of different patterns of postnatal growth and hypothesized that early growth failure leads to impaired development and insulin resistance. Rat pups were separated at birth into normal (N, n = 10) or restricted intake (R, n = 16) litters. At d11, R pups were re-randomized into litters of 6 (R-6), 10 (R-10) or 16 (R-16) pups/dam. N pups remained in litters of 10 pups/dam (N-10). Memory and learning were examined through T-maze test. Insulin sensitivity was measured by i.p. insulin tolerance test and glucose tolerance test. RESULTS: By d10, N pups weighed 20% more than R pups (p < 0.001). By d15, the R-6 group caught up to the N-10 group in weight, the R-10 group showed partial catch-up growth and the R-16 group showed no catch-up growth. All R groups showed poorer scores in developmental testing when compared with the N-10 group during T-Maze test (p < 0.05). Although R-16 were more insulin sensitive than R-6 and R-10, all R groups were more glucose tolerant than N-10. CONCLUSION: In rats, differences in postnatal growth restriction leads to changes in development and in insulin sensitivity. These results may contribute to better elucidating the causes of poor developmental outcomes in human preterm infants.


Asunto(s)
Animales Recién Nacidos/crecimiento & desarrollo , Animales Recién Nacidos/metabolismo , Animales , Animales Recién Nacidos/psicología , Glucemia/metabolismo , Composición Corporal , Peso Corporal , Encéfalo/metabolismo , Ingestión de Alimentos , Femenino , Homeostasis , Insulina/sangre , Masculino , Aprendizaje por Laberinto , Proteína Básica de Mielina/metabolismo , Ratas
6.
bioRxiv ; 2023 Oct 13.
Artículo en Inglés | MEDLINE | ID: mdl-37873395

RESUMEN

Altered tryptophan catabolism has been identified in inflammatory diseases like rheumatoid arthritis (RA) and spondyloarthritis (SpA), but the causal mechanisms linking tryptophan metabolites to disease are unknown. Using the collagen-induced arthritis (CIA) model we identify alterations in tryptophan metabolism, and specifically indole, that correlate with disease. We demonstrate that both bacteria and dietary tryptophan are required for disease, and indole supplementation is sufficient to induce disease in their absence. When mice with CIA on a low-tryptophan diet were supplemented with indole, we observed significant increases in serum IL-6, TNF, and IL-1ß; splenic RORγt+CD4+ T cells and ex vivo collagen-stimulated IL-17 production; and a pattern of anti-collagen antibody isotype switching and glycosylation that corresponded with increased complement fixation. IL-23 neutralization reduced disease severity in indole-induced CIA. Finally, exposure of human colon lymphocytes to indole increased expression of genes involved in IL-17 signaling and plasma cell activation. Altogether, we propose a mechanism by which intestinal dysbiosis during inflammatory arthritis results in altered tryptophan catabolism, leading to indole stimulation of arthritis development. Blockade of indole generation may present a novel therapeutic pathway for RA and SpA.

7.
J Clin Invest ; 134(4)2023 Dec 19.
Artículo en Inglés | MEDLINE | ID: mdl-38113112

RESUMEN

Altered tryptophan catabolism has been identified in inflammatory diseases like rheumatoid arthritis (RA) and spondyloarthritis (SpA), but the causal mechanisms linking tryptophan metabolites to disease are unknown. Using the collagen-induced arthritis (CIA) model, we identified alterations in tryptophan metabolism, and specifically indole, that correlated with disease. We demonstrated that both bacteria and dietary tryptophan were required for disease and that indole supplementation was sufficient to induce disease in their absence. When mice with CIA on a low-tryptophan diet were supplemented with indole, we observed significant increases in serum IL-6, TNF, and IL-1ß; splenic RORγt+CD4+ T cells and ex vivo collagen-stimulated IL-17 production; and a pattern of anti-collagen antibody isotype switching and glycosylation that corresponded with increased complement fixation. IL-23 neutralization reduced disease severity in indole-induced CIA. Finally, exposure of human colonic lymphocytes to indole increased the expression of genes involved in IL-17 signaling and plasma cell activation. Altogether, we propose a mechanism by which intestinal dysbiosis during inflammatory arthritis results in altered tryptophan catabolism, leading to indole stimulation of arthritis development. Blockade of indole generation may present a unique therapeutic pathway for RA and SpA.


Asunto(s)
Artritis Experimental , Artritis Reumatoide , Microbiota , Ratones , Humanos , Animales , Interleucina-17/genética , Interleucina-17/metabolismo , Triptófano , Artritis Reumatoide/genética , Colágeno
8.
Gut Microbes ; 13(1): 1-20, 2021.
Artículo en Inglés | MEDLINE | ID: mdl-33583319

RESUMEN

Inflammatory bowel disease (IBD) coincides with profound shifts in microbiota and host metabolic energy supply and demand. The gastrointestinal epithelium is anatomically positioned to provide a selective barrier between the anaerobic luminal microbiota and host lamina propria, with the microbiota and epithelium participating in an intricate energy exchange necessary for homeostasis. Maintenance and restoration of the barrier requires high energy flux and places significant demands on available substrates to generate ATP. It is recently appreciated that components of the microbiota contribute significantly to a multitude of biochemical pathways within and outside of the mucosa. Decades-old studies have appreciated that byproducts of the microbiota provide essential sources of energy to the intestinal epithelium, especially the colon. More recent work has unveiled the existence of numerous microbial-derived metabolites that support energy procurement within the mucosa. It is now appreciated that disease-associated shifts in the microbiota, termed dysbiosis, places significant demands on energy acquisition within the mucosa. Here, we review the topic of host- and microbial-derived components that influence tissue energetics in health and during disease.


Asunto(s)
Disbiosis/metabolismo , Enfermedades Inflamatorias del Intestino/metabolismo , Butiratos/metabolismo , Creatina/metabolismo , Disbiosis/microbiología , Disbiosis/patología , Metabolismo Energético , Microbioma Gastrointestinal/fisiología , Homeostasis , Inflamación , Enfermedades Inflamatorias del Intestino/microbiología , Enfermedades Inflamatorias del Intestino/patología , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patología , Purinas/metabolismo
9.
Mucosal Immunol ; 14(2): 479-490, 2021 03.
Artículo en Inglés | MEDLINE | ID: mdl-33004979

RESUMEN

Primary sclerosing cholangitis (PSC) is a progressive fibrosing cholestatic liver disease that is strongly associated with inflammatory bowel disease (IBD). PSC-associated IBD (PSC-IBD) displays a unique phenotype characterized by right-side predominant colon inflammation and increased risk of colorectal cancer compared to non-PSC-IBD. The frequent association and unique phenotype of PSC-IBD suggest distinctive underlying disease mechanisms from other chronic liver diseases or IBD alone. Multidrug resistance protein 2 knockout (Mdr2-/-) mice develop spontaneous cholestatic liver injury and fibrosis mirroring human PSC. As a novel model of PSC-IBD, we treated Mdr2-/- mice with dextran sulfate sodium (DSS) to chemically induce colitis (Mdr2-/-/DSS). Mdr2-/- mice demonstrate alterations in fecal bile acid composition and enhanced colitis susceptibility with increased colonic adhesion molecule expression, particularly mucosal addressin-cell adhesion molecule 1 (MAdCAM-1). In vitro, ursodeoxycholic acid (UDCA) co-treatment resulted in a dose dependent attenuation of TNF-α-induced endothelial MAdCAM-1 expression. In the combined Mdr2-/-/DSS model, UDCA supplementation attenuated colitis severity and downregulated intestinal MAdCAM-1 expression. These findings suggest a potential mechanistic role for alterations in bile acid signaling in modulating MAdCAM-1 expression and colitis susceptibility in cholestasis-associated colitis. Together, our findings provide a novel model and new insight into the pathogenesis and potential treatment of PSC-IBD.


Asunto(s)
Ácidos y Sales Biliares/metabolismo , Moléculas de Adhesión Celular/metabolismo , Colangitis Esclerosante/metabolismo , Colestasis/metabolismo , Colitis/metabolismo , Colon/metabolismo , Enfermedades Inflamatorias del Intestino/metabolismo , Mucoproteínas/metabolismo , Subfamilia B de Transportador de Casetes de Unión a ATP/genética , Animales , Moléculas de Adhesión Celular/genética , Colon/patología , Sulfato de Dextran , Modelos Animales de Enfermedad , Susceptibilidad a Enfermedades , Humanos , Ratones , Ratones Noqueados , Mucoproteínas/genética , Factor de Necrosis Tumoral alfa/metabolismo , Ácido Ursodesoxicólico/metabolismo , Miembro 4 de la Subfamilia B de Casete de Unión a ATP
10.
Mucosal Immunol ; 13(2): 230-244, 2020 03.
Artículo en Inglés | MEDLINE | ID: mdl-31792360

RESUMEN

Acute intestinal inflammation includes the early accumulation of neutrophils (PMN). Based on recent evidence that PMN infiltration "imprints" changes in the local tissue environment through local oxygen depletion and the release of adenine nucleotides, we hypothesized that the interaction between transmigrating PMN and intestinal epithelial cells (IECs) results in inflammatory acidification of the tissue. Using newly developed tools, we revealed that active PMN transepithelial migration (TEM) significantly acidifies the local microenvironment, a decrease of nearly 2 pH units. Using unbiased approaches, we sought to define acid-adaptive pathways elicited by PMN TEM. Given the significant amount of adenosine (Ado) generated during PMN TEM, we profiled the influence of Ado on IECs gene expression by microarray and identified the induction of SLC26A3, the major apical Cl-/HCO3- exchanger in IECs. Utilizing loss- and gain-of-function approaches, as well as murine and human colonoids, we demonstrate that Ado-induced SLC26A3 promotes an adaptive IECs phenotype that buffers local pH during active inflammation. Extending these studies, chronic murine colitis models were used to demonstrate that SLC26A3 expression rebounds during chronic DSS-induced inflammation. In conclusion, Ado signaling during PMN TEM induces an adaptive tissue response to inflammatory acidification through the induction of SLC26A3 expression, thereby promoting pH homeostasis.


Asunto(s)
Acidosis/inmunología , Antiportadores/metabolismo , Colitis/inmunología , Inflamación/inmunología , Mucosa Intestinal/fisiología , Intestinos/inmunología , Neutrófilos/inmunología , Transportadores de Sulfato/metabolismo , Acidosis/inducido químicamente , Adaptación Fisiológica , Adenosina/metabolismo , Animales , Antiportadores/genética , Células Cultivadas , Colitis/inducido químicamente , Modelos Animales de Enfermedad , Humanos , Enfermedades del Sistema Inmune , Inflamación/inducido químicamente , Trastornos Leucocíticos , Ratones , Activación Neutrófila , Dodecil Sulfato de Sodio , Transportadores de Sulfato/genética , Migración Transendotelial y Transepitelial , Regulación hacia Arriba
11.
Gut Microbes ; 10(6): 654-662, 2019.
Artículo en Inglés | MEDLINE | ID: mdl-31062653

RESUMEN

Vitamin B12 is a critical nutrient for humans as well as microbes. Due to saturable uptake, high dose oral B12 supplements are largely unabsorbed and reach the distal gut where they are available to interact with the microbiota. The aim of this study was to determine if oral B12 supplementation in mice alters 1) the concentration of B12 and related corrinoids in the distal gut, 2) the fecal microbiome, 3) short chain fatty acids (SCFA), and 4) susceptibility to experimental colitis. C57BL/6 mice (up to 24 animals/group) were supplemented with oral 3.94 µg/ml cyanocobalamin (B12), a dose selected to approximate a single 5 mg supplement for a human. Active vitamin B12 (cobalamin), and four B12-analogues ([ADE]CN-Cba, [2Me-ADE]CN-Cba, [2MeS-ADE]CN-Cba, CN-Cbi) were analyzed in cecal and fecal contents using liquid chromatography/mass spectrometry (LC/MS), in parallel with evaluation of fecal microbiota, cecal SCFA, and susceptibility to dextran sodium sulfate (DSS) colitis. At baseline, active B12 was a minor constituent of overall cecal (0.86%) and fecal (0.44%) corrinoid. Oral B12 supplementation increased active B12 at distal sites by >130-fold (cecal B12 increased from 0.08 to 10.60 ng/mg, fecal B12 increased from 0.06 to 7.81 ng/ml) and reduced microbe-derived fecal corrinoid analogues ([ADE]CN-Cba, [2Me-ADE]CN-Cba, [2MeS-ADE]CN-Cba). Oral B12 had no effect on cecal SCFA. Microbial diversity was unaffected by this intervention, however a selective decrease in Bacteroides was observed with B12 treatment. Lastly, no difference in markers of DSS-induced colitis were detected with B12 treatment.


Asunto(s)
Bacteroides/efectos de los fármacos , Corrinoides/análisis , Suplementos Dietéticos/análisis , Vitamina B 12/administración & dosificación , Complejo Vitamínico B/administración & dosificación , Administración Oral , Animales , Bacteroides/crecimiento & desarrollo , Ciego/química , Colitis/inducido químicamente , Colitis/dietoterapia , Sulfato de Dextran/toxicidad , Ácidos Grasos Volátiles/análisis , Heces/química , Heces/microbiología , Microbioma Gastrointestinal/efectos de los fármacos , Ratones Endogámicos C57BL , Vitamina B 12/farmacología , Complejo Vitamínico B/farmacología
12.
PLoS One ; 12(6): e0179713, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28662197

RESUMEN

BACKGROUND: Iron deficiency is common during infancy and therefore iron supplementation is recommended. Recent reports suggest that iron supplementation in already iron replete infants may adversely affect growth, cognitive development, and morbidity. METHODS: Normal and growth restricted rat pups were given iron daily (30 or 150 µg/d) from birth to postnatal day (PD) 20, and followed to PD56. At PD20, hematology, tissue iron, and the hepatic metabolome were measured. The plasma metabolome and colonic microbial ecology were assessed at PD20 and PD56. T-maze (PD35) and passive avoidance (PD40) tests were used to evaluate cognitive development. RESULTS: Iron supplementation increased iron status in a dose-dependent manner in both groups, but no significant effect of iron on growth was observed. Passive avoidance was significantly lower only in normal rats given high iron compared with controls. In plasma and liver of normal and growth-restricted rats, excess iron increased 3-hydroxybutyrate and decreased several amino acids, urea and myo-inositol. While a profound difference in gut microbiota of normal and growth-restricted rats was observed, with iron supplementation differences in the abundance of strict anaerobes were observed. CONCLUSION: Excess iron adversely affects cognitive development, which may be a consequence of altered metabolism and/or shifts in gut microbiota.


Asunto(s)
Animales Recién Nacidos , Cognición , Crecimiento , Intestinos/microbiología , Metabolómica , Animales , Relación Dosis-Respuesta a Droga , Femenino , Microbiota , Embarazo , Ratas , Ratas Sprague-Dawley
13.
J Mol Med (Berl) ; 95(9): 905-913, 2017 Sep.
Artículo en Inglés | MEDLINE | ID: mdl-28528514

RESUMEN

The intestinal mucosa provides a selective barrier between the anaerobic lumen and a highly metabolic lamina propria. A number of recent studies indicate that acute inflammation of the mucosa can result in tissue hypoxia and associated shifts in tissue metabolism. The activation of hypoxia-inducible factor (HIF) under these conditions has been demonstrated to function as an endogenous molecular cue to promote resolution of inflammation, particularly through the orchestration of barrier repair toward homeostasis. Given the central role of oxygen in tissue metabolism, ongoing studies have defined metabolic endpoints of HIF stabilization as important biomarkers of disease activity. Such findings make HIF and HIF-associated metabolic pathways particularly attractive therapeutic targets in inflammatory bowel disease (IBD). Here, we review the recent literature related to tissue metabolism in IBD.


Asunto(s)
Metabolismo Energético , Enfermedades Inflamatorias del Intestino/etiología , Enfermedades Inflamatorias del Intestino/metabolismo , Mucosa Intestinal/metabolismo , Enfermedad Aguda , Adenosina/metabolismo , Animales , Susceptibilidad a Enfermedades , Microbioma Gastrointestinal/inmunología , Humanos , Hipoxia/metabolismo , Factor 1 Inducible por Hipoxia/metabolismo , Enfermedades Inflamatorias del Intestino/patología , Mucosa Intestinal/inmunología , Mucosa Intestinal/microbiología , Mucosa Intestinal/patología , Especificidad de Órganos , Triptófano/metabolismo
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