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1.
Arch Virol ; 164(10): 2537-2543, 2019 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-31309291

RESUMEN

In May 2017, many free-ranging dorcas gazelles (Gazella dorcas) with suspected signs of peste des petits ruminants (PPR) were reported in Dinder National Park, South-Eastern Sudan. Peste des petits ruminants virus (PPRV) antigen and nucleic acid were detected in specimens from these gazelles using an immunocapture ELISA and a reverse transcription polymerase chain reaction (RT-PCR) assays. PPRV was also detected in four healthy semi-captive dorcas gazelles from two areas of Khartoum State. Phylogenetic analysis showed that these PPRV strains belonged to the lineage IV genotype. The present study demonstrates that gazelles are a potential wild small ruminant host for PPRV and may influence the epidemiology of PPR in the Sudan.


Asunto(s)
Antílopes/virología , Reservorios de Enfermedades , Peste de los Pequeños Rumiantes/virología , Virus de la Peste de los Pequeños Rumiantes/aislamiento & purificación , Animales , Antígenos Virales/aislamiento & purificación , Ensayo de Inmunoadsorción Enzimática , Genotipo , Virus de la Peste de los Pequeños Rumiantes/clasificación , Virus de la Peste de los Pequeños Rumiantes/genética , Filogenia , ARN Viral/aislamiento & purificación , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Análisis de Secuencia de ADN , Gastropatías , Sudán
2.
Trop Anim Health Prod ; 51(3): 655-659, 2019 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-30357603

RESUMEN

During 2015 and 2016, from five different States of the Sudan, a total of 1000 cattle sera were purposively collected from many herds of apparently healthy cattle regardless of their age, sex, and breed. Assessment of the sero-prevalence of PPRV antibodies using competitive ELISA (C-ELISA) yielded a higher overall sero-prevalence of 42.0% (420/1000) among cattle populations in the Sudan which is higher than previously reported. Within Sudanese States under study, the highest sero-prevalence of 53.5% (107/200 sera) was demonstrated in Khartoum State while the least sero-prevalence of 31.5% (63/200 sera) was demonstrated in White Nile State. The higher PPRV sero-prevalence values detected in cattle suggested the potential exposure of cattle to PPRV via contact with infected small ruminants and thus might be an indicator of infection of small ruminants. There is a need to include serological surveillance of PPR in cattle within the sero-monitoring program of PPR to give a better indication of the national herd immunity and to assess in the ongoing eradication program.


Asunto(s)
Peste de los Pequeños Rumiantes/epidemiología , Virus de la Peste de los Pequeños Rumiantes/aislamiento & purificación , Animales , Anticuerpos Antivirales/sangre , Bovinos , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Masculino , Peste de los Pequeños Rumiantes/virología , Prevalencia , Estudios Seroepidemiológicos , Sudán/epidemiología
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