RESUMEN
Jejunal diverticula, like other intestinal diverticula, can become complicated and present as acute abdomen. Diagnosis is difficult and management in complicated cases can be surgical as well as conservative. We present two cases of complicated jejunal diverticulosis that presented with acute abdomen and were managed surgically. Post-operative recovery was satisfactory. Jejunal diverticula is a diagnostic challenge in a low-resource peripheral hospital.
Asunto(s)
Abdomen Agudo , Divertículo , Enfermedades del Yeyuno , Humanos , Enfermedades del Yeyuno/complicaciones , Enfermedades del Yeyuno/diagnóstico , Enfermedades del Yeyuno/cirugía , Divertículo/complicaciones , Divertículo/diagnóstico , Divertículo/cirugía , Yeyuno/cirugía , Hospitales de EnseñanzaRESUMEN
Vicilin has nutraceutical potential and different noteworthy medicative health-promoting biotic diversions, and it is remarkable against pathogenic microorganisms and insects. In this study, Vigna aconitifolia vicilin (VacV) has been identified and characterized from the seed of Vigna aconitifolia (Jacq.) Marechal (Moth beans). LC-MS/MS analysis of VacV provided seven random fragmented sequences comprising 238 residues, showing significant homology with already reported Vigna radiata vicilin (VraV). VacV was purified using ammonium sulfate precipitation (60%) followed by size exclusion chromatography on Hi-Load 16/60 Superdex 200 pg column and anion-exchange chromatography (Hi trap Q FF column). Purified VacV showed a major ~50 kDa band and multiple lower bands on 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) under both reduced and non-reduced conditions. After all, a three-dimensional molecular structure of VacV was predicted, which showed ß-sheeted molecular conformation similar to crystallographic structure of VraV. All Vicilins from V. aconitifolia and other plants were divided into six sub-groups by phylogenetic analysis, and VacV shared a high degree of similarity with vicilins of Vigna radiata, Pisum sativum, Lupinus albus, Cicer arietinum and Glycine max. Additionally, VacV (20 µg) has significant growth inhibition against different pathogenic bacteria along strong antifungal activity (50 µg). Likewise, VacV (3.0 mg) produced significant growth reduction in Rice Weevil Sitophilus oryzae larvae after 9 days compared with control. Furthermore, by using MMT assay, the cytotoxicity effect of VacV on the growth of HepG2 liver cancerous cells was tested. VacV showed cytotoxicity against the HepG-2 line and the acquired value was 180 µg after 48 h. Finally, we performed molecular docking against caspase-3 protein (PDB ID: 3DEI) for VacV bioactive receptor interface residues. Hence, our results reveal that VacV, has nutraceutical potential and moth beans can be used as a rich resource of functional foods.
Asunto(s)
Antiinfecciosos , Insecticidas , Vigna , Antibacterianos/análisis , Antiinfecciosos/análisis , Antiinfecciosos/farmacología , Cromatografía Liquida , Insecticidas/análisis , Insecticidas/farmacología , Simulación del Acoplamiento Molecular , Filogenia , Proteínas de Plantas/química , Proteínas de Almacenamiento de Semillas , Semillas/química , Espectrometría de Masas en TándemRESUMEN
Pakistan ranked highest with reference to average temperatures in cotton growing areas of the world. The heat waves are becoming more intense and unpredictable due to climate change. Identification of heat tolerant genotypes requires comprehensive screening using molecular, physiological and morphological analysis. Heat shock proteins play an important role in tolerance against heat stress. In the current study, eight heat stress responsive factors, proteins and genes (HSFA2, GHSP26, GHPP2A, HSP101, HSC70-1, HSP3, APX1 and ANNAT8) were evaluated morphologically and physiologically for their role in heat stress tolerance. For this purpose, cotton crop was grown at two temperature conditions i.e. normal weather and heat stress at 45 °C. For molecular analysis, genotypes were screened for the presence or absence of heat shock protein genes. Physiological analysis of genotypes was conducted to assess net photosynthesis, stomatal conductance, transpiration rate, leaf-air temperature and cell membrane stability under control as well as high temperature. The traits photosynthesis, cell membrane stability, leaf-air temperature and number of heat stress responsive factors in each genotypes showed a strong correlation with boll retention percentage under heat stress. The genotypes with maximum heat shock protein genes such as Cyto-177, MNH-886, VH-305 and Cyto-515 showed increased photosynthesis, stomatal conductance, negative leaf-air temperature and high boll retention percentage under heat stress condition. These varieties may be used as heat tolerant breeding material.
Asunto(s)
Gossypium/genética , Respuesta al Choque Térmico/genética , Fotosíntesis/genética , Hojas de la Planta/genética , Clorofila/genética , Sequías , Genotipo , Gossypium/crecimiento & desarrollo , Calor , Pakistán , Fitomejoramiento , Hojas de la Planta/crecimiento & desarrolloRESUMEN
With the inception of high voltage (HV), requisites on the insulating permanence of HV equipment is becoming increasingly crucial. Mineral/synthetic oil liquid insulation-together with solid insulation materials (paper, pressboard)-is the fundamental insulation constituent in HV apparatuses; their insulation attributes perform a substantial part in a reliable and steady performance. Meanwhile, implications on the environment, scarcity of petroleum oil supplies and discarding complications with waste oil have stimulated investigators to steer their attention towards sustainable, renewable, biodegradable and environmentally friendly insulating substances. The contemporary insulating constituent's evolution is driven by numerous dynamics-in particular, environmental obligations and other security and economic issues. Consequently, HV equipment manufacturers must address novel specifications concerning to these new standards. Renewable, sustainable and environmentally friendly insulating materials are continuously substituting conventional insulating items in the market place. These are favorable to traditional insulating materials, due to their superior functionality. The also offer explicit security and eco-friendly advantages. This article discusses cutting-edge technology of environmentally friendly insulating materials, including their fabrication, processing and characterization. The new renewable, insulating systems used in HV equipment are submitted and their fundamental gains stated in comparison with conventional insulating materials. Several experimental efforts carried out in various parts of the world are presented, offering an outline of the existing research conducted on renewable insulating systems. The significance of this article lies in summarizing prior investigations, classifying research essence, inducements and predicting forthcoming research trends. Furthermore, opportunities and constraints being experienced in the field of exploration are evidently reported. Last but not least, imminent research proposals and applications are recommended.
RESUMEN
The protective activity of N-(2-hydroxyphenyl)acetamide (NA-2) and NA-2-coated gold nanoparticles (NA-2-AuNPs) in glycerol-treated model of acute kidney injury (AKI) in mice was investigated. NA-2 (50 mg/kg) and NA-2-AuNPs (30 mg/kg) were given to the animals for four days followed by 24-h water deprivation and injection of 50% glycerol (10 ml/kg im). The animals were sacrificed on the next day. Blood and kidneys were collected for biochemical investigations (urea and creatinine), histological studies (hematoxylin and eosin; and periodic acid-Schiff staining), immunohistochemistry (actin and cyclooxygenase-2, Cox-2), and real-time RT-PCR (inducible nitric oxide synthase, iNOS; nuclear factor-κB p50, NFκB; hemeoxygenase-1, HO-1; and kidney injury molecule-1, Kim-1). NA-2 protected renal tubular necrosis and inflammation, though the result of NA-2-AuNPs was better than compound alone and it also exhibited the activity at far less dose. The test compound and its gold nano-formulation decreased the levels of serum urea and creatinine level in the treated animals. Both NA-2 and NA-2-AuNPs also conserved actin cytoskeleton, and lowered COX-2 protein expression. Moreover, the mRNA expressions of iNOS and NFkB p50 were down-regulated, and HO-1 and Kim-1 genes were up-regulated. We conclude that NA-2 and NA-2-AuNPs ameliorates kidney inflammation and injury in glycerol-induced AKI animal model via anti-oxidant and anti-inflammatory mechanisms which make it a suitable candidate for further studies. We believe that these findings will contribute in the understanding of the mechanism of action of paracetamol-like drugs and can be considered for clinical research for the prevention of AKI.
Asunto(s)
Acetanilidas/farmacología , Lesión Renal Aguda/prevención & control , Glicerol/toxicidad , Oro/química , Inflamación/prevención & control , Nanopartículas del Metal/administración & dosificación , Estrés Oxidativo/efectos de los fármacos , Rabdomiólisis/prevención & control , Lesión Renal Aguda/inducido químicamente , Lesión Renal Aguda/metabolismo , Animales , Apoptosis , Crioprotectores/toxicidad , Modelos Animales de Enfermedad , Inflamación/metabolismo , Masculino , Nanopartículas del Metal/química , Ratones , Ratones Endogámicos BALB C , Rabdomiólisis/inducido químicamente , Rabdomiólisis/metabolismoRESUMEN
Small-to-medium scale smart buildings are an important part of the Internet of Things (IoT). Wireless Sensor Networks (WSNs) are the major enabler for smart control in such environments. Reliability is among the key performance requirements for many loss-sensitive IoT and WSN applications, while Energy Consumption (EC) remains a primary concern in WSN design. Error-prone links, traffic intense applications, and limited physical resources make it challenging to meet these service goals-not only that these performance metrics often conflict with one another, but also require solving optimization problems, which are intrinsically NP-hard. Correctly forecasting Packet Delivery Ratio (PDR) and EC can play a significant role in different loss-sensitive application environments. With the ever-increasing availability of performance data, data-driven techniques are becoming popular in such settings. It is observed that a number of communication parameters like transmission power, packet size, etc., influence metrics like PDR and EC in diverse ways. In this work, different regression models including linear, gradient boosting, random forest, and deep learning are used for the purpose of predicting both PDR and EC based on such communication parameters. To evaluate the performance, a public dataset of the IEEE 802.15.4 network, containing measurements against more than 48,000 combinations of parameter configurations, is used. Results are evaluated using root mean square error and it turns out that deep learning achieves up to 98% accuracy for both PDR and EC predictions. These prediction results can help configure communication parameters taking into account the performance goals.
RESUMEN
Light microscopy and electron microscopy have been successfully used in the study of microbes, as well as free-living protists. Unlike light microscopy, which enables us to observe living organisms or the electron microscope which provides a two-dimensional image, atomic force microscopy provides a three-dimensional surface profile. Here, we observed two free-living amoebae, Acanthamoeba castellanii and Balamuthia mandrillaris under the phase contrast inverted microscope, transmission electron microscope and atomic force microscope. Although light microscopy was of lower magnification, it revealed functional biology of live amoebae such as motility and osmoregulation using contractile vacuoles of the trophozoite stage, but it is of limited value in defining the cyst stage. In contrast, transmission electron microscopy showed significantly greater magnification and resolution to reveal the ultra-structural features of trophozoites and cysts including intracellular organelles and cyst wall characteristics but it only produced a snapshot in time of a dead amoeba cell. Atomic force microscopy produced three-dimensional images providing detailed topographic description of shape and surface, phase imaging measuring boundary stiffness, and amplitude measurements including width, height and length of A. castellanii and B. mandrillaris trophozoites and cysts. These results demonstrate the importance of the application of various microscopic methods in the biological and structural characterization of the whole cell, ultra-structural features, as well as surface components and cytoskeleton of protist pathogens.
Asunto(s)
Acanthamoeba castellanii/ultraestructura , Balamuthia mandrillaris/ultraestructura , Oocistos/ultraestructura , Trofozoítos/ultraestructura , Acanthamoeba castellanii/fisiología , Balamuthia mandrillaris/fisiología , Imagenología Tridimensional , Microscopía de Fuerza Atómica , Microscopía Electrónica de Transmisión , Microscopía de Contraste de Fase , Oocistos/fisiología , Trofozoítos/fisiologíaRESUMEN
The introduction of dwarfing genes triggered a wave of "green revolution". A number of wheats dwarfing genes have been reported in previous studies, and only a small fraction of these have been applied to production practices. Therefore, the development of novel dwarfing genes for wheat is of great value. In this study, a novel dwarfing site, Rht-yz, identified in the Yanzhan mutation, is located on chromosome 4B (30-33MB) and its mechanism of action is different from that of Rht-B1b (C-T mutation), but whether it affects the Rht-B1a (TraesCS4B02G043100) or other genes is unclear. Exogenously applied GA3 experiments showed that Rht-yz is one of the gibberellin-insensitive dwarf genes. The effects of the dwarf gene Rht-yz on agronomic traits in wheat were evaluated in the field using Yanzhan, Yanzhan mutations, F2:3 and F3:4 lines. The results showed that Rht-yz improved lodging resistance by reducing plant height, increasing diameter, wall thickness and mechanical strength of the basal stem. In terms of yield traits, Rht-yz had negative effects on tiller number plant-1, biomass plant-1 and yield plant-1, but had no significant effect on harvest index, 1000-kernel weight and spike traits. In addition, Rht-yz significantly increased crude protein, wet gluten and starch content. Therefore, the rational use of the new dwarfing site Rht-yz has potential and value in dwarf wheat breeding.
RESUMEN
Semiconductor-based photocatalysis has attracted significant interest due to its capacity to directly exploit solar energy and generate solar fuels, including water splitting, CO2 reduction, pollutant degradation, and bacterial inactivation. However, achieving the maximum efficiency in photocatalytic processes remains a challenge owing to the speedy recombination of electron-hole pairs and the limited use of light. Therefore, significant endeavours have been devoted to addressing these issues. Specifically, well-designed heterojunction photocatalysts have been demonstrated to exhibit enhanced photocatalytic activity through the physical distancing of electron-hole pairs generated during the photocatalytic process. In this review, we provide a systematic discussion ranging from fundamental mechanisms to material strategies, focusing on TiO2-based heterojunction photocatalysts. Current efforts are focused on developing heterojunction photocatalysts based on TiO2 for a variety of photocatalytic applications, and these projects are explained and assessed. Finally, we offer a concise summary of the main insights and challenges in the utilization of TiO2-based heterojunction photocatalysts for photocatalysis. We expect that this review will serve as a valuable resource to improve the efficiency of TiO2-based heterojunctions for energy generation and environmental remediation.
RESUMEN
Leafy vegetables are considered to have health-promoting potentials, mainly attributed to bioactive phenolic compounds. The antidiabetic effects of spinach, mustard, and cabbage were studied by feeding their phenolic-rich aqueous extracts to alloxan-induced diabetic mice. The antioxidant, biochemical, histopathological, and hematological indices of the control, diabetic, and treated mice were studied. Phenolic compounds present in the extracts were identified and quantified using HPLC-DAD. Results showed ten, nineteen, and eleven phenolic compounds in spinach, mustard, and cabbage leave aqueous extracts, respectively. The body weight, tissue total glutathione (GSH) contents, fasting blood sugar, liver function tests, renal function tests, and lipid profile of the mice were affected by diabetes and were significantly improved by the extract treatments. Likewise, hematological indices and tissues histological studies also showed recovery from diabetic stress in treated mice. The study's findings highlight that the selected leafy vegetables potentially mitigate diabetic complications. Among the studied vegetables, cabbage extract was comparatively more active in ameliorating diabetic stress.
RESUMEN
A series of 6 novel ester derivatives 2-7 of natural product geodin 1 were designed and semi-synthesized through one mild step reaction with high yield. Compounds 2-7 showed strong inhibitory activities against Staphylococcus aureus in the range of 2.35-9.41 µM. Compounds 4 and 7 showed very strong inhibitory activities against antifouling bacteria Aeromonas salmonicida with MICs of 2.42 µM and 4.56 µM respectively. Most notably compounds 3-7 showed potent antifungal activities against Candida albicans in the range of 0.59-2.44 µM. Particularly, compound 3 showed the highest antifungal activity against C. albicans with a MIC value of 0.59 µM. The preliminary structure activity relationship of these derivatives showed that replacement of 4-OH group with benzoyl substituents could enhance the antibacterial and antifungal activities of geodin 1.
RESUMEN
The acceptance of battery electric vehicles (BEVs) is continuously increasing to mitigate CO2 emissions, resulting in an increase in the future material demand for LIBs. Therefore, the proper handling of End-of-life (EOL) BEV batteries requires careful attention to mitigate the supply chain issues for future LIBs materials, especially in Asia. A system dynamics model assessment was performed to evaluate the EOL of LIBs by considering the dynamic lifespan, recovery rate, and economic value under three growth rate scenarios in Asia from 2022 to 2030, depending on the battery chemistry over time. We find that comparing three different scenarios to materials demand, the result showed that materials demand for LIBs is greater in higher scenarios as compared with lower and reference scenarios. Moreover, in the low scenario, the nickel demand and recovery from end-of-life LIBs BEVs will achieve 244.0 and 43.28 kt in 2030. Based on the dynamic economic evaluation, an overall, higher potential economic value of all materials would achieve around 1471 million USD in 2030 in the low scenario. This study manifested that recycling LIBs materials has enormous economic potential and would be a step towards economic sustainability, especially in Asia in the near future.
RESUMEN
Argasid ticks have the vectorial potential for transmitting disease-causing pathogens to avian hosts, resulting in economic losses that may not be fully estimated. Borrelia species are the responsible agents of borreliosis in poultry, animals and humans. Our previous studies have reported a high prevalence of Argas persicus infesting domestic fowls in Khyber Pakhtunkhwa (KP), Pakistan. However, molecular screening and genetic characterization of Borrelia spp. in A. persicus have been neglected in Pakistan. In this study, we focused on the molecular epidemiology and genetic characterization of Borrelia spp. associated with A. persicus ticks infesting domestic fowls and ducks, and Carios vespertilionis infesting bats in selected districts of KP. Overall, 1818 ticks, including females (415; 23%), males (345; 19%), nymphs (475; 26%) and larvae (583; 32%), were collected from 27 locations in nine districts (Peshawar, Mardan, Swabi, Charsadda, Chitral, Lakki Marwat, Bannu, Bajaur and Hangu) from domestic fowls, ducks and their shelters, and bats. A subset of 197 ticks was selected for DNA extraction and PCR to amplify fragments of the cytochrome c oxidase (cox) gene for ticks and flagellin B (flaB) for the detection and genetic characterization of associated Borrelia spp. Among these, only Borrelia anserina DNA was detected in 40 ticks (27.2%) of different life stages, where highest prevalence was found in female ticks (18; 45%), followed by nymphs (12; 30%), larvae (7; 17.5%) and males (3; 7.5%). Tick infestation in shelters (1081; 77%) was higher than on hosts (323; 23%). The resultant cox amplicons of A. persicus showed 100% identity with the same species reported from Pakistan, China, Iran, Kenya, Kazakhstan, Algeria and Egypt and C. vespertilionis show 100% identity with the species reported from Pakistan, China, Japan, Kenya, Vietnam, Spain, Netherlands, the United Kingdom and Hungry, and clustered with the aforementioned species in the phylogenetic tree. The obtained Borrelia sequences showed 100% identity with B. anserina and revealed a close resemblance to the relapsing fever group and clustered in a monophyletic clade with B. anserina from India, Iran and Brazil in a phylogenetic tree. These results establish the first molecular characterization of B. anserina in A. persicus infesting domestic fowls and ducks in the region, as well as their shelters. To effectively control zoonotic consequences, country-wide surveillance research should be encouraged to screen soft ticks infesting various birds for associated pathogens.
RESUMEN
To investigate the mechanism for drought promoting soluble sugar accumulation will be conducive to the enhancement of citrus fruit quality as well as stress tolerance. Fruit sucrose mainly derives from source leaves. Its accumulation in citrus fruit cell vacuole involves in two processes of unloading in the fruit segment membrane (SM) and translocating to the vacuole of fruit juice sacs (JS). Here, transcript levels of 47 sugar metabolism- and transport-related genes were compared in fruit SM or JS between drought and control treatments. Results indicated that transcript levels of cell wall invertase genes (CwINV2/6) and sucrose synthase genes (SUS2/6) in the SM were significantly increased by the drought. Moreover, transcript levels of SWEET genes (CsSWEET1/2/4/5/9) and monosaccharide transporter gene (CsPMT3) were significantly increased in SM under drought treatment. On the other hand, SUS1/3 and vacuolar invertase (VINV) transcript levels were significantly increased in JS by drought; CsPMT4, sucrose transporter gene 2 (CsSUT2), tonoplast monosaccharide transporter gene 2 (CsTMT2), sugar transport protein gene 1 (CsSTP1), two citrus type I V-PPase genes (CsVPP1, and CsVPP2) were also significantly increased in drought treated JS. Collectively, the imposition of drought stress resulted in more soluble sugar accumulation through enhancing sucrose download by enhancing sink strength- and transport ability-related genes, such as CwINV2/6, SUS2/6, CsSWEET1/2/4/5/9, and CsPMT3, in fruit SM, and soluble sugar storage ability by increasing transcript levels of genes, such as CsPMT4, VINV, CsSUT2, CsTMT2, CsSTP1, CsVPP1, and CsVPP2, in fruit JS.
Asunto(s)
Citrus , Azúcares , Azúcares/metabolismo , Frutas/metabolismo , Citrus/genética , Citrus/metabolismo , Sequías , beta-Fructofuranosidasa/genética , beta-Fructofuranosidasa/metabolismo , Carbohidratos , Sacarosa/metabolismo , Proteínas de Transporte de Membrana/genética , Monosacáridos/metabolismo , Regulación de la Expresión Génica de las PlantasRESUMEN
The ß-cyclocitric acid (ß-CCA) is a bioactive apocarotenoid previously shown to improve drought tolerance in annual plants. However, the underlying molecular mechanism of this process remains largely elusive. Moreover, the question about the activity of ß-CCA in perennial fruit crops is still open. Here, we found that treatment of ß-CCA enhances drought tolerance in peach seedlings. The application of ß-CCA significantly increased the relative water content and root activity and reduced the electrolyte leakage of peach seedlings under drought stress. Moreover, treatment with ß-CCA under drought stress increased chlorophyll fluorescence, indicating a positive effect on photosynthesis, while also enhancing superoxide dismutase and peroxidase activity and reducing reactive oxygen species (ROS) levels. Consistent with these alterations, transcriptome analysis revealed an up-regulation of photosynthesis and antioxidant-related genes upon the application of ß-CCA under drought stress. We also detected an induction in genes related to detoxification, environmental adaptation, primary metabolism, phytohormone, phenylpropanoid and the biosynthesis of cutin, suberine and wax, which might contribute to the induction of drought resistance. Altogether, our study reveals that ß-CCA positively modulates peach drought tolerance, which is mainly mediated by enhancing photosynthesis and reducing ROS, indicating the potential of utilizing ß-CCA for drought control in peach and perhaps other fruit crops.
Asunto(s)
Prunus persica , Prunus persica/metabolismo , Resistencia a la Sequía , Plantones/genética , Plantones/metabolismo , Especies Reactivas de Oxígeno/metabolismo , Fotosíntesis/fisiología , Antioxidantes/metabolismo , Sequías , Estrés Fisiológico/genéticaRESUMEN
Organochlorine pesticides (OCPs) have been used extensively as insecticides and herbicides. This study investigates the occurrence of lindane in surface water from the Peshawar valley (i.e., Peshawar, Charsadda, Nowshera, Mardan and Swabi districts of Khyber Pakhtunkhwa, Pakistan). Out of 75 samples tested (i.e., 15 samples from each district), 13 samples (including 2 from Peshawar, 3 from Charsadda, 4 from Nowshera, 1 from Mardan, and 3 from Swabi) are found to be contaminated with lindane. Overall, the detection frequency is 17.3%. The maximum concentration of lindane is detected in a water sample from Nowshera and found to be 2.60 µg L-1. Furthermore, the degradation of lindane in the water sample from Nowshera, containing the maximum concentration, is investigated by simulated solar-light/TiO2 (solar/TiO2), solar/H2O2/TiO2 and solar/persulfate/TiO2 photocatalysis. The degradation of lindane by solar/TiO2 photocatalysis is 25.77% after 10 h of irradiation. The efficiency of the solar/TiO2 process is significantly increased in the presence of 500 µM H2O2 and 500 µM persulfate (PS) (separately), represented by 93.85 and 100.00% lindane removal, respectively. The degradation efficiency of lindane is lower in natural water samples as compared to Milli-Q water, attributed to water matrix effect. Moreover, the identification of degradation products (DPs) shows that lindane follows similar degradation pathways in natural water samples as the one in Milli-Q water. The results show that the occurrence of lindane in surface waters of Peshawar valley is a matter of great concern for human beings and the environment. Interestingly, H2O2 and PS assisted solar/TiO2 photocatalysis is an effective method for the removal of lindane from natural water.
RESUMEN
Waterlogging is a major abiotic stress that plants encounter as a result of climate change impacts. Peach is very sensitive to hypoxia during waterlogging, which causes poor tree vigor and huge economic losses. The molecular mechanism underlying the peach response to waterlogging and reoxygenation remains unclear. Here, the physiological and molecular responses of 3-week-old peach seedlings under waterlogged and recovery conditions were comprehensively analyzed. As a result, waterlogging significantly reduced plant height and biomass with inhibition of root growth when compared with control and reoxygenation. Similar results were observed for photosynthetic activities and gaseous exchange parameters. Waterlogging increased lipid peroxidation, hydrogen peroxide, proline, glutamic acid and glutathione contents, while superoxide dismutase, peroxidases and catalase activities were decreased. The glucose and fructose contents were accumulated, contrary to sucrose which was reduced remarkably throughout the stress periods. The level of endogenous indole acetic acid (IAA) was increased in waterlogging but decreased after reoxygenation. However, the change trends of jasmonic acid (JA), cytokinins and abscisic acid (ABA) levels were opposite to IAA. In transcriptomic analysis, there were 13,343 differentially expressed genes (DEGs) with higher and 16,112 genes with lower expression. These DEGs were greatly enriched in carbohydrate metabolism, anaerobic fermentation, glutathione metabolism and IAA hormone biosynthesis under waterlogging, while they were significantly enriched in photosynthesis, reactive oxygen species scavenging, ABA and JA hormones biosynthesis in reoxygenation. Moreover, several genes related to stress response, carbohydrate metabolism and hormones biosynthesis were significantly changed in waterlogging and reoxygenation, which indicated unbalanced amino acid, carbon and fatty acid pools in peach roots. Taken together, these results suggest that glutathione, primary sugars and hormone biosynthesis and signaling might play key roles in plant response to waterlogging. Our work provides a comprehensive understanding of gene regulatory networks and metabolites in waterlogging stress and its recuperation, which will facilitate peach waterlogging control.
Asunto(s)
Prunus persica , Prunus persica/metabolismo , Transcriptoma , Ácido Abscísico/metabolismo , Plantas/metabolismo , Glutatión , HormonasRESUMEN
Nanotechnology has recently appeared as an important study subject in modern material sciences. Greener synthesis of nanoparticles has gained the attention of many scientists because of its integral characteristics such as effectiveness, eco-friendly, and low cost. In the present study by following the green synthesis approach, zinc oxide nanoparticles (ZnO NPs) were formed for the very first time by using Senecio chrysanthemoides leaf extract as a reducing agent. The UV-Vis spectrophotometer was used to study the synthesized ZnO NPs, and the specific peak was found to be at 349 nm. The characteristic Fourier transform infrared (FTIR) peak was found to be at 449 cm-1 which displays the peak of ZnO molecules. The surface morphology of the ZnO NPs was determined via scanning electron microscopy (SEM). The energy-dispersive X-ray spectroscopy (EDX) study showed that the synthesized ZnO NPs are present at the weight percentage of 66.38%. The X-ray diffraction (XRD) spectrum confirmed the hexagonal phase wurtzite structure, with the average particle size of 31 nm, and demonstrated the crystalline structure of ZnO NPs. Additionally, to all these experiments, we compared the anti-inflammatory properties of biogenic ZnO NPs to a standard drug. Biosynthesized ZnO NPs have revealed an effective anti-inflammatory activity at a higher concentration (100 mL-1) and showed 73% inhibition in comparison with diclofenac sodium drug. Zinc oxide was shown to be compatible with diclofenac sodium, according to the results. The ZnO NPs produced using the greener synthesis process have the potential to be used in a broad range of fields and also used as a good anti-inflammatory agent.
Asunto(s)
Nanopartículas del Metal , Nanopartículas , Senecio , Óxido de Zinc , Óxido de Zinc/farmacología , Óxido de Zinc/química , Antibacterianos/farmacología , Diclofenaco , Pruebas de Sensibilidad Microbiana , Nanopartículas/química , Difracción de Rayos X , Extractos Vegetales/farmacología , Extractos Vegetales/química , Nanopartículas del Metal/química , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Dihydropyrazole (1-22) derivatives were synthesized from already synthesized chalcones. The structures of all of the synthesized compounds were confirmed by elemental analysis and various spectroscopic techniques. Furthermore, the synthesized compounds were screened against α amylase as well as investigated for antioxidant activities. The synthesized compounds demonstrate good to excellent antioxidant activities with IC50 values ranging between 30.03 and 913.58 µM. Among the 22 evaluated compounds, 11 compounds exhibit excellent activity relative to the standard ascorbic acid IC50 = 287.30 µM. Interestingly, all of the evaluated compounds show good to excellent α amylase activity with IC50 values lying in the range between 0.5509 and 810.73 µM as compared to the standard acarbose IC50 = 73.12 µM. Among the investigated compounds, five compounds demonstrate better activity compared to the standard. In order to investigate the binding interactions of the evaluated compounds with amylase protein, molecular docking studies were conducted, which show an excellent docking score as compared to the standard. Furthermore, the physiochemical properties, drug likeness, and ADMET were investigated, and it was found that none of the compounds violate Lipiniski's rule of five, which shows that this class of compounds has enough potential to be used as a drug candidate in the near future.
RESUMEN
The fibroblast growth factor receptor 3 (FGFR3) is warranted as a promising therapeutic target in bladder cancer as it is described in 75% of papillary bladder tumors. Considering this, the present study was conducted to use different approaches of computer-aided drug discovery (CADD) to identify the best binding compounds against the active pocket of FGFR3. Compared to control pyrimidine derivative, the study identified three promising lead structures; BDC_24037121, BDC_21200852, and BDC_21206757 with binding energy value of -14.80 kcal/mol, -12.22 kcal/mol, and -11.67 kcal/mol, respectively. The control molecule binding energy score was -9.85 kcal/mol. The compounds achieved deep pocket binding and produced balanced interactions of hydrogen bonds and van der Waals. The FGFR3 enzyme residues such as Leu478, Lys508, Glu556, Asn562, Asn622, and Asp635. The molecular dynamic (MD) simulation studies additionally validated the docked conformation stability with respect to FGFR3 with a mean root mean square deviation (RMSD) value of < 3 Å. The root mean square fluctuation (RMSF) complements the complexes structural stability and the residues showed less fluctuation in the presence of compounds. The Poisson-Boltzmann or generalized Born and surface area continuum solvation (MM/PBSA and MM/GBSA) methods revalidated compounds better binding and highlighted van der Waals energy to dominate the overall net energy. The docked stability was additionally confirmed by WaterSwap and AMBER normal mode entropy energy analyses. In a nutshell, the compounds shortlisted in this study are promising in term of theoretical binding affinity for FGFR3 but experimental validation is needed.Communicated by Ramaswamy H. Sarma.