RESUMEN
Staphylococcus aureus is the most frequent pathogen causing diabetic foot infections. Here, we investigated the degree of bacterial virulence required to establish invasive tissue infections in diabetic organisms. Staphylococcal isolates from diabetic and non-diabetic foot ulcers were tested for their virulence in in vitro functional assays of host cell invasion and cytotoxicity. Isolates from diabetes mellitus type I/II patients exhibited less virulence than isolates from non-diabetic patients, but were nevertheless able to establish severe infections. In some cases, non-invasive isolates were detected deep within diabetic wounds, even though the strains were non-pathogenic in cell culture models. Testing of defined isolates in murine footpad injection models revealed that both low- and high-virulent bacterial strains persisted in higher numbers in diabetic compared to non-diabetic hosts, suggesting that hyperglycemia favors bacterial survival. Additionally, the bacterial load was higher in NOD mice, which have a compromised immune system, compared to C57Bl/6 mice. Our results reveal that high as well as low-virulent staphylococcal strains are able to cause soft tissue infections and to persist in diabetic humans and mice, suggesting a reason for the frequent and endangering infections in patients with diabetes.
Asunto(s)
Carga Bacteriana , Diabetes Mellitus Experimental/patología , Pie Diabético/microbiología , Infecciones de los Tejidos Blandos/microbiología , Staphylococcus aureus/patogenicidad , Adulto , Anciano , Animales , Células Cultivadas , Células Endoteliales de la Vena Umbilical Humana , Humanos , Hiperglucemia/metabolismo , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos NOD , Persona de Mediana Edad , Staphylococcus aureus/aislamiento & purificación , Estreptozocina , VirulenciaRESUMEN
INTRODUCTION: New implant designs like the Proximal Femoral Nail Antirotation (PFNA) were developed to reduce failure rates in unstable pertrochanteric fractures in the elderly. Standardized implant augmentation with up to 6â¯mL of polymethylmethacrylate (PMMA) cement has been introduced to enhance implant anchorage by increasing the implant-bone interface in osteoporotic bone conditions. Biomechanically, loads to failure were significantly higher with augmentation. The primary objective of this study was to compare the mobility of patients with closed unstable trochanteric fractures treated by PFNA either with or without cement augmentation. PATIENTS AND METHODS: A prospective multicentre, randomized, patient-blinded trial was conducted with ambulatory patients aged 75 or older who sustained a closed, unstable trochanteric fracture. Surgical fixation had to be performed within 72â¯h after admission. Outcomes were evaluated at baseline, during surgery, 3 to 14â¯days after surgery, 3 months, 6 months, and 12 months after surgery. To evaluate the primary objective, patients' walking speed was assessed by the Timed Up and Go (TUG) test. Secondary objectives included the analysis of implant migration assessed on radiographs, quality of life measured by the Barthel Index, mobility measured by the Parker Mobility Score, and complications. RESULTS: Of 253 randomized patients, 223 patients were eligible: 105 patients were allocated to the PFNA Augmentation group and 118 to PFNA group. At 3 to 14â¯days after surgery, there was no statistical significant difference in mean walking speed between the treatment groups. For the secondary objectives, also no statistical significant differences were found. However, no patient in the PFNA Augmentation group had a reoperation due to mechanical failure or symptomatic implant migration compared to 6 patients in the PFNA group. CONCLUSIONS: Augmentation of the PFNA blade did not improve patients' walking ability compared to the use of a non-augmented PFNA but might have the potential to prevent reoperations by strengthening the osteosynthesis construct.
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Cementos para Huesos/uso terapéutico , Cementoplastia/métodos , Fijación Intramedular de Fracturas , Fracturas de Cadera/cirugía , Polimetil Metacrilato/uso terapéutico , Rango del Movimiento Articular/fisiología , Anciano , Anciano de 80 o más Años , Clavos Ortopédicos , Femenino , Fijación Intramedular de Fracturas/métodos , Fracturas de Cadera/fisiopatología , Humanos , Masculino , Estudios Prospectivos , Resultado del TratamientoRESUMEN
There is a shortage of orthopaedic surgeons in Malawi. Orthopaedic clinical officers (OCOs) treat trauma patients and occasionally perform major orthopaedic surgery. No studies have assessed the efficacy and safety of their work. The aim of this study was to evaluate their contribution to major orthopaedic surgery at Zomba Central Hospital. Data about orthopaedic procedures during 2006-2010 were collected from theatre books. We selected major amputations and open reductions and plating for outcome analysis and collected details from files. We compared patients operated by OCOs alone ('OCOs alone' group) and by surgeons or OCOs assisted by surgeons ('Surgeon present' group). OCOs performed 463/1010 major (45.8%) and 1600/1765 minor operations (90.7%) alone. There was no difference in perioperative outcome between both groups. OCOs carry out a large proportion of orthopaedic procedures with good clinical results. Shifting of clinical tasks including major orthopaedic surgery can be safe. Further prospective studies are recommended.
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Técnicos Medios en Salud/normas , Atención a la Salud/normas , Ortopedia/normas , Calidad de la Atención de Salud/normas , Heridas y Lesiones/cirugía , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Niño , Preescolar , Atención a la Salud/estadística & datos numéricos , Países en Desarrollo , Femenino , Humanos , Malaui/epidemiología , Masculino , Persona de Mediana Edad , Ortopedia/estadística & datos numéricos , Evaluación de Resultado en la Atención de Salud , Estudios ProspectivosRESUMEN
We identified 33 Malawians who had undergone total splenectomy for traumatic injury. We reviewed these and 33 controls by clinical and parasitological examination monthly for 1 year. Splenectomized patients (S) were 2.5 times as likely as controls (C) to complain about febrile symptoms during the month preceding a visit (P < 0.0001). They were nearly twice as likely as controls to have Plasmodium falciparum parasitaemia (S: 176/283 person visits; C: 86/262; P < 0.0001). Parasitaemia was more likely to be associated with febrile symptoms in splenectomized individuals (S: 104/176, 59%; C: 24/86, 28%; P < 0.0001). There were three deaths (two non-malarial, one unexplained) among splenectomized subjects and none in the control group. Parasite densities reached significantly higher levels, and mature parasite stages were more often seen in the peripheral blood, in asplenic individuals. In a partially immune population, asplenic individuals are at increased risk of malarial infections and illness. In a larger group without the benefit of regular review and prompt therapy, there may be an increased risk of life-threatening malaria. Splenectomy should be avoided when possible in an area with endemic transmission of P. falciparum.
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Malaria Falciparum/epidemiología , Complicaciones Posoperatorias/etiología , Esplenectomía , Adolescente , Adulto , Anciano , Animales , Estudios de Casos y Controles , Niño , Susceptibilidad a Enfermedades , Femenino , Humanos , Estudios Longitudinales , Malaui/epidemiología , Masculino , Persona de Mediana Edad , Plasmodium falciparum/aislamiento & purificación , Complicaciones Posoperatorias/epidemiología , Estudios ProspectivosRESUMEN
OBJECTIVES: This study examined the potential for measuring dynamic inducible micromotion (DIMM) between fragments in healing distal radial fractures using radiostereometry (RSA). DESIGN: Prospective imaging study. SETTING: University teaching hospital. PATIENTS: Nine patients with low-impact distal radial fractures. INTERVENTION: Volar locked plating of the fracture with insertion of tantalum beads into bone fragments. RSA examinations at 1 day and then 2, 6, 26, and 52 weeks. Motion at the fracture site was induced by maximal voluntary hand grip using a Jamar dynamometer. Radiographs were analyzed using locally developed and UMRSA software. MAIN OUTCOME MEASUREMENTS: DIMM and migration were calculated as translations and rotations of the main distal segment. Clinical precision was assessed under repeatability conditions. RESULTS: Precision (as 95% error limit) ranged from 0.06 to 0.13 mm and 0.5 to 0.8 degrees for migration, and from 0.10 to 0.14 mm and 0.6 to 1.0 degrees for DIMM. DIMM was characterized by axial and dorsal compression with dorsiflexion. The median DIMM of patients reached a maximum at 2 weeks: mainly as 0.3 mm axial compression, 0.3 mm dorsal compression, and 2.5 degrees dorsiflexion. DIMM ceased by 26 weeks, indicating union of all fractures. Fracture collapse continued until the 26-week measurement, ranging between 0.2 and 2.8 mm axially. Instability of some intraosseous markers was observed. CONCLUSIONS: The precision of this RSA method was sufficient to observe inducible movements occurring during fracture healing. This has the potential for quantifying rates of fracture union and improving understanding of the available treatments.
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Imagenología Tridimensional/métodos , Interpretación de Imagen Radiográfica Asistida por Computador/métodos , Fracturas del Radio/diagnóstico por imagen , Fracturas del Radio/cirugía , Tomografía Computarizada por Rayos X/métodos , Traumatismos de la Muñeca/diagnóstico por imagen , Traumatismos de la Muñeca/cirugía , Curación de Fractura , Humanos , Movimiento (Física)RESUMEN
A 12-year-old boy who was overrun by a train, sustained traumatic bilateral above-knee amputation and a rupture of the symphysis. The left leg had multiple fractures and soft tissue injuries and amputation was necessary. The right one, although severely crushed, at the amputation site and with a MESS of 9, was replanted accepting some shortening and a soft tissue defect at the amputation site, employing saphenic vein grafts from the amputate (left leg) and an early free latissimus dorsi-flap. Septic complications at the amputation site were managed, and an autologous sciatic nerve graft was performed 8 months after the accident, employing the contralateral above-knee stump as the donor. Protective foot sole sensitivity was noticed after 2 years and 4 months and continued to improve. Further reconstructive procedures included ORIF of a femoral fracture in the contra-lateral stump. On the replanted leg proximal tibia corrective osteotomy and lateral collateral knee ligament reconstruction were performed. A follow-up of 7 years and 9 months demonstrates now a leg capable of full weight bearing and recovery of overall protective sensitivity. The boy made good psycho-social progress after difficulties and feels that the replanted leg is of significantly greater use to him than the hi-tech prosthesis on the other leg.
RESUMEN
Mesosigmoidoplasty is a nonresective procedure for nongangrenous sigmoid volvulus. It aims to correct the main pathogenetic factor of this condition, the narrow but long mesosigmoid. Although it had been demonstrated by intraoperative measurements that this has indeed achieved its purpose, recurrent volvulus has been reported after the procedure. This has raised the question of whether an operation on the mesosigmoid's peritoneal cover alone is sufficient to maintain the change in shape. The modification presented here aims at more sustainable shortening and widening of the mesosigmoid by including all its layers in the procedure. Of 31 consecutive patients with sigmoid volvulus operated on in Malawi in East Africa, 14 had the nongangrenous variety. Twelve of them underwent modified mesosigmoidoplasty without any major perioperative complications. There was one relaparotomy following misdiagnosis of cryptic Hirschsprung's disease and one true recurrence secondary to a technical error during the previous operation. During a follow-up of eight patients for 4 to 8 months, there were no complaints about abdominal discomfort or changed stool habits. Thus including all layers of the mesosigmoid in the plastic procedure did not increase short-term morbidity and in theory should be advantageous in respect to preventing recurrence. Applying it to a larger series with prolonged follow-up seems to be justified.
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Vólvulo Intestinal/cirugía , Mesocolon/cirugía , Enfermedades del Sigmoide/cirugía , Procedimientos Quirúrgicos del Sistema Digestivo/métodos , Estudios de Seguimiento , HumanosRESUMEN
BACKGROUND: Laser-induced fluorescence angiography with indocyanine-green (ICG-FA) is a new diagnostic approach that allows quantitative evaluation of tissue perfusion in microsurgery as well as an analysis of the uptake, distribution, and clearance of dye-marked blood. METHODS: The ICG-FA technique was evaluated for its sensitivity and prognostic value in comparison with clinical parameters (turgor, temperature, reperfusion time, bleeding after puncture) during and after different surgical procedures. Altogether, 91 measurements were made in cases of microvascular repair (n = 43) and free-flap surgery (n = 48). RESULTS: Because of discrepancies between ICG-FA results and clinical findings, the planned postoperative management was changed 43 times (47.2%). In cases of microvascular repair that subsequently resulted in tissue necrosis, ICG fluorescence had a significantly higher prognostic value than any of the clinical parameters (p = 0.03). During free-flap surgery, intraoperative ICG-FA had better sensitivity for flap loss than clinical parameters, but postoperative ICG-FA did not. CONCLUSIONS: Fluorescence angiography with ICG is a sensitive diagnostic tool for detecting compromised tissue perfusion in trauma surgery and microsurgery. Its use may improve perioperative management and thereby lead to better clinical results.