RESUMEN
Past studies have established mesophyll diffusion conductance to CO2 (gm ) as a variable and significant limitation to plant photosynthesis under steady-state conditions. However, the role of gm in influencing photosynthesis (A) during the transient period of light induction is largely unknown. We combined gas exchange measurements with laser-enabled carbon isotope discrimination measurements to assess gm during photosynthetic induction, using Arabidopsis as the measurement species. Our measurements revealed three key findings: (1) we found that the rate at which gm approached steady state during induction was not necessarily faster than the induction rate of the carboxylation process, contradictory to what has been suggested in previous studies; (2) gm displayed a strong and consistent coordination with A under both induction and steady-state settings, hinting that the mechanism driving gm -A coupling does not require physiological stability as a prerequisite; and (3) photosynthetic limitation analysis of our data revealed that when integrated over the entire induction period, the relative limitation of A imposed by gm can be as high as > 35%. The present study provides the first demonstration of the important role of gm in limiting CO2 assimilation during photosynthetic induction, thereby pointing to a need for more research attention to be devoted to gm in future induction studies.
Asunto(s)
Arabidopsis , Células del Mesófilo , Dióxido de Carbono , Difusión , Fotosíntesis , Hojas de la PlantaRESUMEN
The leaf intercellular airspace is a tortuous environment consisting of cells of different shapes, packing densities, and orientation, all of which have an effect on the travelling distance of molecules from the stomata to the mesophyll cell surfaces. Tortuosity, the increase in displacement over the actual distance between two points, is typically defined as encompassing the whole leaf airspace, but heterogeneity in pore dimensions and orientation between the spongy and palisade mesophyll likely result in heterogeneity in tortuosity along different axes and would predict longer traveling distance along the path of least tortuosity, such as vertically within the columnar cell matrix of the palisade layer. Here, we compare a previously established geometric method to a random walk approach, novel for this analysis in plant leaves, in four different Eucalyptus species. The random walk method allowed us to quantify directional tortuosity across the whole leaf profile, and separately for the spongy and palisade mesophyll. For all species tortuosity was higher in the palisade mesophyll than the spongy mesophyll and horizontal (parallel to the epidermis) tortuosity was consistently higher than vertical (from epidermis to epidermis) tortuosity. We demonstrate that a random walk approach improves on previous geometric approaches and is valuable for investigating CO2 and H2 O transport within leaves.
Asunto(s)
Eucalyptus/anatomía & histología , Hojas de la Planta/anatomía & histología , Hojas de la Planta/citología , Aire , Isótopos de Carbono/análisis , Pared Celular/ultraestructura , Eucalyptus/ultraestructura , Imagenología Tridimensional , Células del Mesófilo/química , Microscopía Electrónica de Rastreo , Células Vegetales , Hojas de la Planta/ultraestructura , Estomas de Plantas/anatomía & histologíaRESUMEN
An expression was earlier derived for the non-steady state isotopic composition of a leaf when the composition of the water entering the leaf was not necessarily the same as that of the water being transpired (Farquhar and Cernusak 2005). This was relevant to natural conditions because the associated time constant is typically sufficiently long to ensure that the leaf water composition and fluxes of the isotopologues are rarely steady. With the advent of laser-based measurements of isotopologues, leaves have been enclosed in cuvettes and time courses of fluxes recorded. The enclosure modifies the time constant by effectively increasing the resistance to the one-way gross flux out of the stomata because transpiration increases the vapour concentration within the chamber. The resistance is increased from stomatal and boundary layer in series, to stomata, boundary layer and chamber resistance, where the latter is given by the ratio of leaf area to the flow rate out of the chamber. An apparent change in concept from one-way to net flux, introduced by Song, Simonin, Loucos and Barbour (2015) is resolved, and shown to be unnecessary, but the value of their data is reinforced.
Asunto(s)
Isótopos de Oxígeno/metabolismo , Hojas de la Planta/metabolismo , Transpiración de Plantas , Agua/metabolismo , Hidrógeno/metabolismo , Modelos Biológicos , Estomas de Plantas/metabolismoRESUMEN
H2 18 O enrichment develops when leaves transpire, but an accurate generalized mechanistic model has proven elusive. We hypothesized that leaf hydraulic architecture may affect the degree to which gradients in H2 18 O develop within leaves, influencing bulk leaf stable oxygen isotope enrichment (ΔL ) and the degree to which the Péclet effect is relevant in leaves. Leaf hydraulic design predicted the relevance of a Péclet effect to ΔL in 19 of the 21 species tested. Leaves with well-developed hydraulic connections between the vascular tissue and the epidermal cells through bundle sheath extensions and clear distinctions between palisade and spongy mesophyll layers (while the mesophyll is hydraulically disconnected) may have velocities of the transpiration stream such that gradients in H2 18 O develop and are expressed in the mesophyll. In contrast, in leaves where the vascular tissue is hydraulically disconnected from the epidermal layers, or where all mesophyll cells are well connected to the transpiration stream, velocities within the liquid transport pathways may be low enough that gradients in H2 18 O are very small. Prior knowledge of leaf hydraulic design allows informed selection of the appropriate ΔL modelling framework.
Asunto(s)
Oxígeno/metabolismo , Fenómenos Fisiológicos de las Plantas , Transpiración de Plantas/fisiología , Plantas/anatomía & histología , Transporte Biológico , Células del Mesófilo/metabolismo , Modelos Biológicos , Isótopos de Oxígeno/análisis , Hojas de la Planta/anatomía & histología , Hojas de la Planta/fisiología , Plantones/anatomía & histología , Plantones/fisiología , Agua/fisiologíaRESUMEN
Variation in temperature (T) is usually accompanied by changes in leaf water potential (Ψleaf ), which may influence mesophyll conductance (gm ). However, the effects of Ψleaf on gm have not yet been considered in models of the gm response to temperature. Temperature responses of gm and Ψleaf and the response of gm to Ψleaf were studied in rice (Oryza sativa) and wheat (Triticum aestivum), and then an empirical model of Ψleaf was incorporated into an existing gm -T model. In wheat, Ψleaf was dramatically decreased with increasing T, whereas in rice Ψleaf was less sensitive or insensitive to T. Without taking Ψleaf into account, gm for wheat showed no response to T. However, at a given Ψleaf , gm was significantly higher at high temperature compared with low. After incorporating the function of Ψleaf into the gm -T model, we suggest that the gm -T relationship can be influenced by the activation and deactivation energy for membrane permeability, Ψleaf gradient between temperatures, and the sensitivity of gm to Ψleaf , below a threshold (Ψleaf,0 ). The data presented here suggest that Ψleaf plays an important role in the gm -T relationship and should be considered in future studies related to the temperature response of gm and photosynthesis.
Asunto(s)
Células del Mesófilo/fisiología , Oryza/fisiología , Temperatura , Agua/fisiología , Gases/metabolismo , Modelos BiológicosRESUMEN
Leaf function is intimately related to the size, shape, abundance and position of cells and chloroplasts. Anatomy has long been assessed and quantified in two dimensions with 3D structure inferred from 2D micrographs. Serial block face scanning electron microscopy (SBF-SEM) was used to reconstruct 95 cells and 1173 chloroplasts from three wheat and nine chickpea leaves (three samples each from three chickpea genotypes). Wheat chloroplast volume was underestimated by 61% in mesophyll cells and 45% in bundle sheath cells from 2D micrographs, whereas chickpea mesophyll chloroplast volume was underestimated by 60% using simple geometrical models. Models of chickpea spongy and palisade cells both under- and overestimated surface area and volume by varying degrees. These models did not adequately capture irregular shapes such as flattening of chloroplasts or lobed spongy mesophyll cells. It is concluded that simple geometrical models to estimate chloroplast and cell 3D volume and surface area from 2D micrographs are inadequate, and that SBF-SEM has strong potential to contribute to improved understanding of leaf form and function.
Asunto(s)
Cloroplastos , Células del Mesófilo , Tamaño de la Célula , Microscopía Electrónica de Rastreo , Hojas de la PlantaRESUMEN
Thermoregulation of leaf temperature (Tleaf ) may foster metabolic homeostasis in plants, but the degree to which Tleaf is moderated, and under what environmental contexts, is a topic of debate. Isotopic studies inferred the temperature of photosynthetic carbon assimilation to be a constant value of c. 20°C; by contrast, leaf biophysical theory suggests a strong dependence of Tleaf on environmental drivers. Can this apparent disparity be reconciled? We continuously measured Tleaf and whole-crown net CO2 uptake for Eucalyptus parramattensis trees growing in field conditions in whole-tree chambers under ambient and +3°C warming conditions, and calculated assimilation-weighted leaf temperature (TL-AW ) across 265 d, varying in air temperature (Tair ) from -1 to 45°C. We compared these data to TL-AW derived from wood cellulose δ18 O. Tleaf exhibited substantial variation driven by Tair , light intensity, and vapor pressure deficit, and Tleaf was strongly linearly correlated with Tair with a slope of c. 1.0. TL-AW values calculated from cellulose δ18 O vs crown fluxes were remarkably consistent; both varied seasonally and in response to the warming treatment, tracking variation in Tair . The leaves studied here were nearly poikilothermic, with no evidence of thermoregulation of Tleaf towards a homeostatic value. Importantly, this work supports the use of cellulose δ18 O to infer TL-AW , but does not support the concept of strong homeothermic regulation of Tleaf.
Asunto(s)
Dióxido de Carbono , Eucalyptus , Árboles , Homeostasis , Isótopos de Oxígeno , Fotosíntesis , Hojas de la Planta , TemperaturaRESUMEN
The temperature response of mesophyll conductance to CO2 diffusion (gm) has been shown to vary considerably between species but remains poorly understood. Here, we tested the hypothesis that increases in chloroplast surface area with increasing temperature, due to the formation of chloroplast protrusions, caused observed positive responses of gm to temperature. We found no evidence of chloroplast protrusions. Using simultaneous measurements of carbon and oxygen isotope discrimination during photosynthesis to separate total gm (gm13) into cell wall and plasma membrane conductance (gm18) and chloroplast membrane conductance (gcm) components, we explored the temperature response in genotypes of soybean and barley, and sunflower plants grown at differing CO2 concentrations. Differences in the temperature sensitivity of gm18 were found between genotypes and between plants grown at differing CO2 concentration but did not relate to measured anatomical features such as chloroplast surface area or cell wall thickness. The closest fit of modelled gm13 to estimated values was found when cell wall thickness was allowed to decline at higher temperatures and transpiration rates, but it remains to be tested if this decline is realistic. The temperature response of gcm (calculated from the difference between 1/gm13 and 1/gm18) varied between barley genotypes, and was best fitted by an optimal response in sunflower. Taken together, these results indicate that gm is a highly complex trait with unpredictable sensitivity to temperature that varies between species, between genotypes within a single species, with growth environment, between replicate leaves, and even with age for an individual leaf.
Asunto(s)
Células del Mesófilo/fisiología , Plantas/genética , Plantas/metabolismo , Temperatura , Dióxido de Carbono/metabolismo , Cloroplastos/metabolismo , Genotipo , Modelos Biológicos , Hojas de la Planta/anatomía & histología , Hojas de la Planta/fisiología , Transpiración de Plantas/fisiología , Especificidad de la EspecieRESUMEN
83 I. 83 II. 84 III. 84 IV. 85 V. 86 VI. 86 VII. 86 87 References 87 SUMMARY: The balance of carbon dioxide (CO2 ) and water vapour exchange between leaves and the atmosphere is strongly controlled by stomatal conductance. However, the influence of transport processes within leaves has recently been gaining prominence. Stable isotope techniques are at the forefront of understanding transport within leaves and the recent development of online, real-time optical isotope analysers has paved the way for new questions to be asked. In this insight, I outline these new techniques and the questions they can potentially address, including assessing possible coordination between mesophyll conductance to CO2 and leaf hydraulic conductance.
Asunto(s)
Carbono/metabolismo , Marcaje Isotópico/métodos , Hojas de la Planta/metabolismo , Agua/metabolismo , Transporte Biológico , Cloroplastos/metabolismo , Hojas de la Planta/genéticaAsunto(s)
Fotosíntesis , Árboles , Isótopos de Carbono , Isótopos de Oxígeno , Hojas de la Planta , AguaRESUMEN
Contents 986 I. 987 II. 987 III. 988 IV. 991 V. 992 VI. 995 VII. 997 VIII. 998 References 998 SUMMARY: It has been 75 yr since leaf respiratory metabolism in the light (day respiration) was identified as a low-flux metabolic pathway that accompanies photosynthesis. In principle, it provides carbon backbones for nitrogen assimilation and evolves CO2 and thus impacts on plant carbon and nitrogen balances. However, for a long time, uncertainties have remained as to whether techniques used to measure day respiratory efflux were valid and whether day respiration responded to environmental gaseous conditions. In the past few years, significant advances have been made using carbon isotopes, 'omics' analyses and surveys of respiration rates in mesocosms or ecosystems. There is substantial evidence that day respiration should be viewed as a highly dynamic metabolic pathway that interacts with photosynthesis and photorespiration and responds to atmospheric CO2 mole fraction. The view of leaf day respiration as a constant and/or negligible parameter of net carbon exchange is now outdated and it should now be regarded as a central actor of plant carbon-use efficiency.
Asunto(s)
Dióxido de Carbono/metabolismo , Hojas de la Planta/metabolismo , Respiración de la Célula , Ecosistema , Nitrógeno/metabolismoRESUMEN
Stomata represent one resistor in a series of resistances for carbon and water exchange between the leaf and the atmosphere; the remaining resistors occurring within the leaf, commonly represented as mesophyll conductance to CO2 , gm , and leaf hydraulic conductance, kLeaf . Recent studies have proposed that gm and kLeaf may be coordinated across species because of shared pathways. We assessed the correlation between gm and kLeaf within cotton, under growth CO2 partial pressure and irradiance treatments and also with short-term variation in irradiance and humidity. gm was estimated using two isotopic techniques that allowed partitioning of total gm (Δ13 C-gm ) into cell wall plus plasma membrane conductance (Δ18 O-gm ) and chloroplast membrane conductance (gcm ). A weak correlation was found between Δ13 C-gm and kLeaf only when measured under growth conditions. However, Δ18 O-gm was related to kLeaf under both short-term environmental variation and growth conditions. Partitioning gm showed that gcm was not affected by short-term changes in irradiance or correlated with kLeaf , but was strongly reduced at high growth CO2 partial pressure. Thus, simultaneous measurements of gm , kLeaf and gcm suggest independent regulation of carbon and water transport across the chloroplast membrane with limited coordinated regulation across the cell wall and plasma membrane.
Asunto(s)
Células del Mesófilo/fisiología , Hojas de la Planta/fisiología , Agua/fisiología , Dióxido de Carbono/farmacología , Isótopos de Carbono , Difusión , Gossypium/anatomía & histología , Gossypium/efectos de los fármacos , Gossypium/crecimiento & desarrollo , Luz , Células del Mesófilo/efectos de los fármacos , Método de Montecarlo , Hojas de la Planta/anatomía & histología , Hojas de la Planta/efectos de los fármacos , Especificidad de la EspecieRESUMEN
The oxygen isotope composition of leaf water imparts a signal to a range of molecules in the atmosphere and biosphere, but has been notoriously difficult to measure in studies requiring a large number of samples as a consequence of the labour-intensive extraction step. We tested a method of direct equilibration of water in fresh leaf samples with CO2 , and subsequent oxygen isotope analysis on an optical spectrometer. The oxygen isotope composition of leaf water measured by the direct equilibration technique was strongly linearly related to that of cryogenically extracted leaf water in paired samples for a wide range of species with differing anatomy, with an R(2) of 0.95. The somewhat more enriched values produced by the direct equilibration method may reflect lack of full equilibration with unenriched water in the vascular bundles, but the strong relationship across a wide range of species suggests that this difference can be adequately corrected for using a simple linear relationship.
Asunto(s)
Marcaje Isotópico/métodos , Isótopos de Oxígeno/metabolismo , Hojas de la Planta/metabolismo , Poaceae/metabolismo , Agua/metabolismo , Zea mays/metabolismo , Destilación , VacioRESUMEN
Mesophyll conductance significantly, and variably, limits photosynthesis but we currently have no reliable method of measurement for C4 plants. An online oxygen isotope technique was developed to allow quantification of mesophyll conductance in C4 plants and to provide an alternative estimate in C3 plants. The technique is compared to an established carbon isotope method in three C3 species. Mesophyll conductance of C4 species was similar to that in the C3 species measured, and declined in both C4 and C3 species as leaves aged from fully expanded to senescing. In cotton leaves, simultaneous measurement of carbon and oxygen isotope discrimination allowed the partitioning of total conductance to the chloroplasts into cell wall and plasma membrane versus chloroplast membrane components, if CO2 was assumed to be isotopically equilibrated with cytosolic water, and the partitioning remained stable with leaf age. The oxygen isotope technique allowed estimation of mesophyll conductance in C4 plants and, when combined with well-established carbon isotope techniques, may provide additional information on mesophyll conductance in C3 plants.
Asunto(s)
Dióxido de Carbono/metabolismo , Fraccionamiento Químico/métodos , Células del Mesófilo/metabolismo , Sistemas en Línea , Oxígeno/metabolismo , Hojas de la Planta/metabolismo , Isótopos de Carbono , Cloroplastos/metabolismo , Isótopos de Oxígeno , Fotosíntesis , Hojas de la Planta/anatomía & histología , Estomas de Plantas/fisiología , Plantas/metabolismoRESUMEN
Leaf water contains naturally occurring stable isotopes of oxygen and hydrogen in abundances that vary spatially and temporally. When sufficiently understood, these can be harnessed for a wide range of applications. Here, we review the current state of knowledge of stable isotope enrichment of leaf water, and its relevance for isotopic signals incorporated into plant organic matter and atmospheric gases. Models describing evaporative enrichment of leaf water have become increasingly complex over time, reflecting enhanced spatial and temporal resolution. We recommend that practitioners choose a model with a level of complexity suited to their application, and provide guidance. At the same time, there exists some lingering uncertainty about the biophysical processes relevant to patterns of isotopic enrichment in leaf water. An important goal for future research is to link observed variations in isotopic composition to specific anatomical and physiological features of leaves that reflect differences in hydraulic design. New measurement techniques are developing rapidly, enabling determinations of both transpired and leaf water δ(18) O and δ(2) H to be made more easily and at higher temporal resolution than previously possible. We expect these technological advances to spur new developments in our understanding of patterns of stable isotope fractionation in leaf water.
Asunto(s)
Hojas de la Planta/fisiología , Plantas/metabolismo , Agua/metabolismo , Isótopos de Oxígeno , Transpiración de Plantas/fisiologíaRESUMEN
The two-pool and Péclet effect models represent two theories describing mechanistic controls underlying leaf water oxygen isotope composition at the whole-leaf level (δ(18) OL ). To test these models, we used a laser spectrometer coupled to a gas-exchange cuvette to make online measurements of δ(18) O of transpiration (δ(18) Otrans ) and transpiration rate (E) in 61 cotton (Gossypium hirsutum) leaves. δ(18) Otrans measurements permitted direct calculation of δ(18) O at the sites of evaporation (δ(18) Oe ) which, combined with values of δ(18) OL from the same leaves, allowed unbiased estimation of the proportional deviation of enrichment of δ(18) OL from that of δ(18) Oe (f) under both steady-state (SS) and non-steady-state (NSS) conditions. Among all leaves measured, f expressed relative to both δ(18) O of transpired water (ftrans ) and source water (fsw ) remained relatively constant with a mean ± SD of 0.11 ± 0.05 and 0.13 ± 0.05, respectively, regardless of variation in E spanning 0.8-9.1 mmol m(-2) s(-1) . Neither ftrans nor fsw exhibited a significant difference between the SS and NSS leaves at the P < 0.05 level. Our results suggest that the simpler two-pool model is adequate for predicting cotton leaf water enrichment at the whole-leaf level. We discuss the implications of adopting a two-pool concept for isotopic applications in ecological studies.
Asunto(s)
Gossypium/fisiología , Modelos Biológicos , Oxígeno/metabolismo , Hojas de la Planta/fisiología , Transpiración de Plantas/fisiología , Agua/metabolismo , Transporte Biológico , Ambiente , Isótopos de Oxígeno/análisisRESUMEN
The combined use of a gas-exchange system and laser-based isotope measurement is a tool of growing interest in plant ecophysiological studies, owing to its relevance for assessing isotopic variability in leaf water and/or transpiration under non-steady-state (NSS) conditions. However, the current Farquhar & Cernusak (F&C) NSS leaf water model, originally developed for open-field scenarios, is unsuited for use in a gas-exchange cuvette environment where isotope composition of water vapour (δv ) is intrinsically linked to that of transpiration (δE ). Here, we modified the F&C model to make it directly compatible with the δv -δE dynamic characteristic of a typical cuvette setting. The resultant new model suggests a role of 'net-flux' (rather than 'gross-flux' as suggested by the original F&C model)-based leaf water turnover rate in controlling the time constant (τ) for the approach to steady sate. The validity of the new model was subsequently confirmed in a cuvette experiment involving cotton leaves, for which we demonstrated close agreement between τ values predicted from the model and those measured from NSS variations in isotope enrichment of transpiration. Hence, we recommend that our new model be incorporated into future isotope studies involving a cuvette condition where the transpiration flux directly influences δv . There is an increasing popularity among plant ecophysiologists to use a gas-exchange system coupled to laser-based isotope measurement for investigating non-steady state (NSS) isotopic variability in leaf water (and/or transpiration); however, the current Farquhar & Cernusak (F&C) NSS leaf water model is unsuited for use in a gas-exchange cuvette environment due to its implicit assumption of isotope composition of water vapor (δv ) being constant and independent of that of transpiration (δE ). In the present study, we modified the F&C model to make it compatible with the dynamic relationship between δv and δE as is typically associated with a cuvette setting. Using an experiment conducted on cotton leaves, we show that the modified NSS model performed well in predicting the time constant for the exponential approach of leaf water toward steady state under cuvette conditions. Such a result demonstrates the applicability of this new model to gas-exchange cuvette conditions where the transpiration flux directly influences δv , and therefore suggests the need to incorporate this model into future isotope studies that employ a laser-cuvette coupled system.
Asunto(s)
Modelos Teóricos , Hojas de la Planta/fisiología , Transpiración de Plantas/fisiología , Isótopos de Oxígeno/análisis , Vapor/análisis , Agua/análisisRESUMEN
Leaf hydraulic conductance (k leaf) is a central element in the regulation of leaf water balance but the properties of k leaf remain uncertain. Here, the evidence for the following two models for k leaf in well-hydrated plants is evaluated: (i) k leaf is constant or (ii) k leaf increases as transpiration rate (E) increases. The difference between stem and leaf water potential (ΔΨstem-leaf), stomatal conductance (g s), k leaf, and E over a diurnal cycle for three angiosperm and gymnosperm tree species growing in a common garden, and for Helianthus annuus plants grown under sub-ambient, ambient, and elevated atmospheric CO2 concentration were evaluated. Results show that for well-watered plants k leaf is positively dependent on E. Here, this property is termed the dynamic conductance, k leaf(E), which incorporates the inherent k leaf at zero E, which is distinguished as the static conductance, k leaf(0). Growth under different CO2 concentrations maintained the same relationship between k leaf and E, resulting in similar k leaf(0), while operating along different regions of the curve owing to the influence of CO2 on g s. The positive relationship between k leaf and E minimized variation in ΔΨstem-leaf. This enables leaves to minimize variation in Ψleaf and maximize g s and CO2 assimilation rate over the diurnal course of evaporative demand.
Asunto(s)
Helianthus/fisiología , Hojas de la Planta/fisiología , Tallos de la Planta/fisiología , Transpiración de Plantas , Agua/metabolismo , Transporte Biológico , Helianthus/química , Cinética , Hojas de la Planta/química , Tallos de la Planta/química , Agua/químicaRESUMEN
Certainty regarding the degree to which organic molecules exchange oxygen with local water during plant cellulose synthesis (p(ex)) is necessary for cellulose oxygen isotope (δ(18)O(cell))-based applications in environmental and ecological studies. However, the currently accepted notion that p(ex) is a constant of ca. 0.42 appears inconsistent with biochemical theory, which predicts that marked variation may be present in p(ex), in relation to variation in the turnover time (τ) of the carbohydrate pool available for cellulose synthesis. The above prediction was tested in the present study with the analysis of data collected from leaves of Ricinus communis grown in controlled environmental conditions that varied in light intensity and vapour pressure deficit. The results revealed the existence of considerable variation in both p(ex) and τ across plants in the various growth environments. Moreover, despite uncertainties in estimates of the proportion of source water in the synthesis water (p(x)) and of the biochemical fractionation factor (ε(o)), our experiment yielded strong evidence that p(ex) exhibits a significant, positive relationship with τ, consistent with biochemical theory. The observed variation in p(ex) in association with τ has important implications for the interpretation of δ(18)O(cell) data in environmental/ecological studies.
Asunto(s)
Celulosa/metabolismo , Hojas de la Planta/metabolismo , Ricinus communis/metabolismo , Australia , Eucalyptus/metabolismo , Isótopos de Oxígeno , Lluvia , Factores de Tiempo , Árboles/metabolismo , Agua/metabolismoRESUMEN
We tested an approach to estimate daily canopy net photosynthesis, A, based on estimates of transpiration, E, using measurements of sap flow and water-use efficiency, ω, by measuring δ13C in CO2 respired from shoots in the canopies of two conifers (Podocarpaceae) native to New Zealand. The trees were planted in adjacent 20-year-old stands with the same soil and environmental conditions. Leaf area index was lower for Dacrycarpus dacrydioides D.Don in Lamb (1.34 m2 m-2) than for Podocarpus totara G.Benn. ex D.Don var. totara (2.01 m2 m-2), but mean (± standard error) stem diameters were the same at 152 ± 21 mm for D. dacrydioides and 154 ± 25 mm for P. totara. Over a 28-day period, daily A (per unit ground area) ranged almost five-fold but there were no significant differences between species (mean 2.73 ± 1.02 gC m-2 day-1). This was attributable to higher daily values of E (2.63 ± 0.83 mm day-1) and lower ω (1.35 ± 0.53 gC kg H2O-1) for D. dacrydioides compared with lower E (1.82 ± 0.72 mm day-1) and higher ω (1.90 ± 0.77 gC kg H2O-1) for P. totara. We attributed this to higher nitrogen availability and nitrogen concentration per unit foliage area, Na, and greater exposure to irradiance in the D. dacrydioides canopy compared with P. totara. Our findings support earlier observations that D. dacrydioides is more adapted to sites with poor drainage. In contrast, the high retention of leaf area and maintaining low rates of transpiration by P. totara, resulting in higher water-use efficiency, is an adaptive response to survival in dry conditions. Our findings show that physiological adjustments for two species adapted to different environments led to similar canopy photosynthesis rates when the trees were grown in the same conditions. We demonstrated consistency between whole-tree and more intensive shoot-scale measurements, confirming that integrated approaches are appropriate for comparative estimates of carbon uptake in stands with different species.