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Nucleic Acids Res ; 49(D1): D1094-D1101, 2021 01 08.
Artículo en Inglés | MEDLINE | ID: mdl-33095860

RESUMEN

Most mutations in cancer genomes occur in the non-coding regions with unknown impact on tumor development. Although the increase in the number of cancer whole-genome sequences has revealed numerous putative non-coding cancer drivers, their information is dispersed across multiple studies making it difficult to understand their roles in tumorigenesis of different cancer types. We have developed CNCDatabase, Cornell Non-coding Cancer driver Database (https://cncdatabase.med.cornell.edu/) that contains detailed information about predicted non-coding drivers at gene promoters, 5' and 3' UTRs (untranslated regions), enhancers, CTCF insulators and non-coding RNAs. CNCDatabase documents 1111 protein-coding genes and 90 non-coding RNAs with reported drivers in their non-coding regions from 32 cancer types by computational predictions of positive selection using whole-genome sequences; differential gene expression in samples with and without mutations; or another set of experimental validations including luciferase reporter assays and genome editing. The database can be easily modified and scaled as lists of non-coding drivers are revised in the community with larger whole-genome sequencing studies, CRISPR screens and further experimental validations. Overall, CNCDatabase provides a helpful resource for researchers to explore the pathological role of non-coding alterations in human cancers.


Asunto(s)
Carcinogénesis/genética , Bases de Datos Genéticas , Regulación Neoplásica de la Expresión Génica , Genoma Humano , Neoplasias/genética , Regiones no Traducidas 3' , Regiones no Traducidas 5' , Carcinogénesis/metabolismo , Carcinogénesis/patología , Repeticiones Palindrómicas Cortas Agrupadas y Regularmente Espaciadas , Elementos de Facilitación Genéticos , Genes Reporteros , Humanos , Elementos Aisladores , Luciferasas/genética , Luciferasas/metabolismo , Mutación , Neoplasias/metabolismo , Neoplasias/patología , Sistemas de Lectura Abierta , Regiones Promotoras Genéticas , ARN no Traducido/clasificación , ARN no Traducido/genética , ARN no Traducido/metabolismo , Regiones no Traducidas , Secuenciación Completa del Genoma
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