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1.
Rheumatol Ther ; 11(1): 143-155, 2024 Feb.
Artículo en Inglés | MEDLINE | ID: mdl-38070102

RESUMEN

INTRODUCTION: Fibroblast-like synoviocytes (FLS) play a critical role in inflammation that contributes to disease progression in juvenile idiopathic arthritis (JIA). In rheumatoid arthritis (RA), FLS express tumor necrosis factor alpha (TNFα). TNFα signaling has been shown to be upstream of transforming growth factor beta (TGFß) signaling. Overexpression of TNFα and TGFß, as well as related proteins, can cause increased inflammation in RA. In this study, we examine the effects of inhibiting TNFα signaling with adalimumab on FLS isolated from synovial fluid of patients with JIA. METHODS: Synovial fluid samples were selected from 41 patients in our repository. Of these samples, 23 were diagnosed with persistent oligoarticular JIA and 18 were diagnosed with extended oligoarticular JIA. All samples were taken prior to patients extending to a polyarticular course, or what we termed extended-to-be (ETB), and were on no medications or nonsteroidal anti-inflammatory drugs (NSAIDs) only at the time of arthrocentesis. For cell studies, FLS were isolated from synovial fluid and treated with adalimumab for 24 h. Protein antibody arrays were performed by RayBiotech, Inc. according to their protocols. RESULTS: In persistent FLS, TNFα (fold change [FC] = 1.2 p = 0.001), TGFß (FC = 1.5 p = 0.001), lymphotoxin alpha (LTα) (FC = 4.3 p = 0.015), soluble tumor necrosis factor receptor 1 (sTNFRI) (FC = 5.1 p = 0.008), and soluble tumor necrosis factor receptor 2 (sTNFRII) (FC = 3.8 p = 0.025) were significantly elevated in adalimumab treated cells compared to untreated cells. In ETB FLS, TNFα was significantly elevated (FC = 1.04 p = 0.023) while TGFß (FC = 1.03 p = 0.037) was significantly decreased in adalimumab-treated cells compared to untreated cells. CONCLUSIONS: This data suggests that, after only 24 h, adalimumab is effective at decreasing inflammation that occurs downstream of initial TNFα signaling in extended-to-be fibroblast-like synoviocytes.

2.
Arthritis Res Ther ; 24(1): 225, 2022 09 27.
Artículo en Inglés | MEDLINE | ID: mdl-36167601

RESUMEN

BACKGROUND: Fibroblast-like synoviocytes (FLS) play a crucial role in JIA pathogenesis; however, the mechanisms by which they contribute to disease progression are not well described. Previous studies demonstrated that rheumatoid arthritis FLS are heterogeneous, and subpopulations with transformed, aggressive phenotypes cause invasive and destructive disease activity. We employ single-cell RNA-sequencing (scRNA-seq) to investigate JIA FLS heterogeneity and gene expression that distinguishes JIA subtypes. METHODS: JIA FLS cell lines from three persistent oligoarticular, three pre-extension oligoarticular, and three polyarticular subtypes were cultured. scRNA-seq was performed by Genewiz according to 10 × Genomics Chromium protocols. SeuratR package was used for QC, analysis, and exploration of data. RESULTS: FLS are heterogeneous and have characteristics of fibroblasts, chondrocytes, and smooth muscle cells. The chondrocyte-like subpopulation is the predominant cell type and percentages of this subpopulation increase with disease severity. Despite overlapping subpopulations, the chondrocyte-like cells have unique genetic fingerprints that distinguish between JIA subtypes. LRRC15, GREM1, and GREM2 are overexpressed in chondrocyte-like cells from persistent oligoarticular JIA FLS compared to pre-extension oligoarticular JIA FLS. S100A4, TIMP3, and NBL1 are overexpressed in pre-extension oligoarticular JIA FLS compared to polyarticular JIA FLS. CRLF1, MFAP5, and TNXB are overexpressed in persistent oligoarticular JIA FLS compared to polyarticular JIA FLS. CONCLUSIONS: We found biologically relevant differences in gene expression between JIA subtypes that support a critical role for FLS in pathogenesis. We also demonstrate that gene expression within the chondrocyte-like subpopulation can be used to distinguish between these subtypes.


Asunto(s)
Artritis Juvenil , Sinoviocitos , Artritis Juvenil/patología , Cromo/metabolismo , Fibroblastos/metabolismo , Humanos , Proteínas de la Membrana/metabolismo , ARN/metabolismo , Análisis de la Célula Individual , Sinoviocitos/metabolismo
3.
Pediatr Rheumatol Online J ; 18(1): 89, 2020 Nov 16.
Artículo en Inglés | MEDLINE | ID: mdl-33198759

RESUMEN

BACKGROUND: To examine critical interactions between juvenile idiopathic arthritis synovial fibroblasts (JFLS) and chondrocytes (Ch), and their role in bony overgrowth seen in patients with juvenile idiopathic arthritis (JIA). METHODS: Control (CFLS) and JFLS were cultured in synoviocyte media containing recombinant BMP4. Ch were cultured in either CFLS or JFLS conditioned-media without stimulation. Media supernatants were analyzed by ELISA. RNA from conditioned media experiment was analyzed by ClariomS microarray. RESULTS: As expected, genes expressed in untreated JFLS and CFLS cultured in synoviocyte media were similar to each other and this expression differed from untreated Ch cultured in chondrocyte media. JFLS favor BMP ligand gene expression while downregulating TGFß receptors' expression. Noggin and chordin, antagonists with high affinity for BMP4, are JFLS- but not Ch-preferred regulators of BMP signaling. Compared to Ch, JFLS overexpress collagen X (COLX), a marker of chondrocyte hypertrophy. Exogenous BMP4 causes JFLS to significantly decrease expression of noggin and collagen II (COL2), a marker of chondrocyte proliferation, and causes overexpression of COLX and alkaline-phosphatase (ALP). Chondrocytes cultured in JFLS-conditioned media (Ch-JFLS) express BMP genes and favor chordin protein expression over other antagonists. Ch-JFLS have significantly increased expression of COL2 and significantly decreased expression of COLX. CONCLUSIONS: These data suggest JFLS, in the presence of BMP4, undergo hypertrophy and that JFLS-conditioned media influence chondrocytes to become highly proliferative. To the authors' knowledge, no prior study has shown that JFLS and chondrocytes play a direct role in the bony overgrowth in joints of patients with JIA and that BMPs or regulation of these growth factors influence the interaction between two prominent synovial cell types.


Asunto(s)
Artritis Juvenil , Proteína Morfogenética Ósea 4 , Proteínas Portadoras , Condrocitos , Hiperostosis/metabolismo , Sinoviocitos , Artritis Juvenil/metabolismo , Artritis Juvenil/patología , Proteína Morfogenética Ósea 4/genética , Proteína Morfogenética Ósea 4/metabolismo , Proteínas Portadoras/genética , Proteínas Portadoras/metabolismo , Comunicación Celular , Diferenciación Celular/genética , Células Cultivadas , Condrocitos/metabolismo , Condrocitos/patología , Colágeno/metabolismo , Regulación de la Expresión Génica , Humanos , Sinoviocitos/metabolismo , Sinoviocitos/patología
4.
J Clin Invest ; 130(4): 1669-1682, 2020 04 01.
Artículo en Inglés | MEDLINE | ID: mdl-31874111

RESUMEN

BACKGROUNDUndifferentiated systemic autoinflammatory diseases (USAIDs) present diagnostic and therapeutic challenges. Chronic interferon (IFN) signaling and cytokine dysregulation may identify diseases with available targeted treatments.METHODSSixty-six consecutively referred USAID patients underwent underwent screening for the presence of an interferon signature using a standardized type-I IFN-response-gene score (IRG-S), cytokine profiling, and genetic evaluation by next-generation sequencing.RESULTSThirty-six USAID patients (55%) had elevated IRG-S. Neutrophilic panniculitis (40% vs. 0%), basal ganglia calcifications (46% vs. 0%), interstitial lung disease (47% vs. 5%), and myositis (60% vs. 10%) were more prevalent in patients with elevated IRG-S. Moderate IRG-S elevation and highly elevated serum IL-18 distinguished 8 patients with pulmonary alveolar proteinosis (PAP) and recurrent macrophage activation syndrome (MAS). Among patients with panniculitis and progressive cytopenias, 2 patients were compound heterozygous for potentially novel LRBA mutations, 4 patients harbored potentially novel splice variants in IKBKG (which encodes NF-κB essential modulator [NEMO]), and 6 patients had de novo frameshift mutations in SAMD9L. Of additional 12 patients with elevated IRG-S and CANDLE-, SAVI- or Aicardi-Goutières syndrome-like (AGS-like) phenotypes, 5 patients carried mutations in either SAMHD1, TREX1, PSMB8, or PSMG2. Two patients had anti-MDA5 autoantibody-positive juvenile dermatomyositis, and 7 could not be classified. Patients with LRBA, IKBKG, and SAMD9L mutations showed a pattern of IRG elevation that suggests prominent NF-κB activation different from the canonical interferonopathies CANDLE, SAVI, and AGS.CONCLUSIONSIn patients with elevated IRG-S, we identified characteristic clinical features and 3 additional autoinflammatory diseases: IL-18-mediated PAP and recurrent MAS (IL-18PAP-MAS), NEMO deleted exon 5-autoinflammatory syndrome (NEMO-NDAS), and SAMD9L-associated autoinflammatory disease (SAMD9L-SAAD). The IRG-S expands the diagnostic armamentarium in evaluating USAIDs and points to different pathways regulating IRG expression.TRIAL REGISTRATIONClinicalTrials.gov NCT02974595.FUNDINGThe Intramural Research Program of the NIH, NIAID, NIAMS, and the Clinical Center.


Asunto(s)
Enfermedades Autoinmunes , Interferón Tipo I , Interleucina-18 , Síndrome de Activación Macrofágica , Mutación , Paniculitis , Proteinosis Alveolar Pulmonar , Enfermedades Autoinmunes/genética , Enfermedades Autoinmunes/inmunología , Femenino , Humanos , Interferón Tipo I/genética , Interferón Tipo I/inmunología , Interleucina-18/genética , Interleucina-18/inmunología , Síndrome de Activación Macrofágica/genética , Síndrome de Activación Macrofágica/inmunología , Masculino , Paniculitis/genética , Paniculitis/inmunología , Proteinosis Alveolar Pulmonar/genética , Proteinosis Alveolar Pulmonar/inmunología
5.
Pediatr Rheumatol Online J ; 16(1): 3, 2018 Jan 08.
Artículo en Inglés | MEDLINE | ID: mdl-29310668

RESUMEN

BACKGROUND: Our intent was to identify differences between the transcriptome of fibroblast-like synoviocytes (FLS) in oligoarticular juvenile idiopathic arthritis (JIA) before extension when compared to persistent subtype of JIA, when the two are clinically indistinguishable. Additionally, we sought to determine if differences between the transcriptomes of FLS from extended-to-be and polyarticular course JIA could be detected. Our hypothesis was that intrinsic differences in the transcriptome of the FLS from extended-to-be JIA would distinguish them from persistent oligoarticular JIA, before the course is clinically apparent. METHODS: Global gene expression was defined in cultured FLS from 6 controls, 12 JIA with persistent course, 7 JIA prior to extension (extended-to-be), 4 JIA with extended course and 6 polyarticular onset, using Affymetrix Human GeneChips 133plus2.0. RESULTS: Bioconductor Linear Models for Microarray Analysis revealed 22 probesets with differential expression between persistent and extended-to-be FLS at 15% FDR, however only 2 probesets distinguished extended-to-be from extended and none distinguished extended-to-be and polyarticular at 15% FDR. Differences in extended and polyarticular gene expression profiles were not detected. Confirmation of select genes was done on the RNA level by RT-qPCR and on the protein level in synovial fluid by ELISA. CONCLUSIONS: The transcriptome of FLS from extended-to-be juvenile idiopathic arthritis is distinct from persistent course before a clinical distinction can be made. Additionally, the transcriptome of extended-to-be and polyarticular course, including those who have already extended, are indistinguishable. These gene expression data suggest that FLS already reflect a polyarticular behavior early in disease course, suggesting that extended-to-be may be "latent polyarticular" at onset. These differences can be used to develop early biomarkers of disease course, allowing for better-informed treatment decisions.


Asunto(s)
Artritis Juvenil/metabolismo , Biomarcadores/metabolismo , Sinoviocitos/metabolismo , Adolescente , Artritis Juvenil/diagnóstico , Artritis Juvenil/genética , Técnicas de Cultivo de Célula , Niño , Preescolar , Progresión de la Enfermedad , Ensayo de Inmunoadsorción Enzimática , Femenino , Humanos , Masculino , Análisis por Micromatrices , Reacción en Cadena en Tiempo Real de la Polimerasa , Líquido Sinovial/citología , Transcriptoma/genética
6.
Clin Rheumatol ; 26(12): 2167-2169, 2007 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-17516128

RESUMEN

Lupus anticoagulant hypoprothrombinemia syndrome (LAHPS) is a rare acquired disorder associated with several different conditions but mostly with systemic lupus erythematosus (SLE). LAHPS probably results from the presence of anti-Factor II antibodies, which usually counterbalance the prothrombotic effect of the lupus anticoagulant (LAC). In fact, Factor II deficiency in SLE is invariably associated with the presence of LAC. No consensus exists for the treatment of LAHPS. Corticosteroids, with or without the addition of vitamin K or blood products, have been a successful first-line treatment. Immunoglobulin (IVIG) treatment has been shown to be effective in the setting of acute bleeding. However, in some patients, conservative treatment is not enough to control bleeding, and the addition of immunosuppressive therapy, usually azathioprine, is needed. In our patients, Factor II deficiency reappeared after tapering steroids. Both children achieved normal Factor II levels with cyclophosphamide. This effect was long-lasting, a phenomenon that has not been documented in children prior to this report.


Asunto(s)
Ciclofosfamida/uso terapéutico , Hipoprotrombinemias/sangre , Inmunosupresores/uso terapéutico , Lupus Eritematoso Sistémico/tratamiento farmacológico , Protrombina/metabolismo , Adolescente , Biomarcadores/sangre , Niño , Femenino , Estudios de Seguimiento , Humanos , Hipoprotrombinemias/tratamiento farmacológico , Hipoprotrombinemias/etiología , Lupus Eritematoso Sistémico/sangre , Lupus Eritematoso Sistémico/complicaciones , Masculino , Síndrome
7.
Proteomics Clin Appl ; 11(5-6)2017 05.
Artículo en Inglés | MEDLINE | ID: mdl-28012239

RESUMEN

PURPOSE: The goal is to investigate the specific contribution of fibroblast-like synoviocytes (FLS) to the inflammatory milieu of the synovium in juvenile idiopathic arthritis (JIA) through detection of secreted proteins. EXPERIMENTAL DESIGN: Expression of 89 cytokines and chemokines is determined on unprocessed synovial fluid from controls and JIA patients using antibody arrays. Supernatants from pure cell cultures of FLS grown from synovial fluids or tissues from JIA and controls are also examined for protein expression. Ingenuity Pathway Analysis (IPA) is revealed top pathways and upstream regulators of significant proteins. RESULTS: Protein studies is revealed that JIA FLS release pro-inflammatory cytokines and chemokines, including IL-4, IL-6, IL-17, CXCL1, and CXCL6, and lose expression of important regulator signals, such as IL-10 and TIMP2. Of the 84 proteins differentially expressed between controls and JIA in the synovial fluid, 1/3 (29 proteins) are differentially expressed in the cell culture supernatants of JIA and control FLS. ELISA of cell culture supernatants and synovial fluid confirmed seven key proteins. CONCLUSION AND CLINICAL RELEVANCE: JIA FLS are central to perpetuation of inflammation in JIA, including trafficking of inflammatory cells and effects on the extracellular matrix. These cells express key disease-specific chemokines that, with further refinement, may allow us to tailor therapy appropriately.


Asunto(s)
Artritis Juvenil/metabolismo , Artritis Juvenil/patología , Quimiocinas/metabolismo , Fibroblastos/patología , Transducción de Señal , Sinoviocitos/metabolismo , Sinoviocitos/patología , Adolescente , Quimiocinas/biosíntesis , Niño , Preescolar , Femenino , Humanos , Inflamación/metabolismo , Masculino , FN-kappa B/metabolismo , Líquido Sinovial/metabolismo
9.
Arthritis Rheumatol ; 66(5): 1352-62, 2014 May.
Artículo en Inglés | MEDLINE | ID: mdl-24782191

RESUMEN

OBJECTIVE: This study was designed to investigate the pathogenic contributions of fibroblast-like synoviocytes (FLS) to juvenile idiopathic arthritis (JIA) by identifying pathways with dysregulated gene expression in FLS from patients with oligoarticular JIA. METHODS: FLS were derived from synovial fluid obtained by arthrocentesis from patients with JIA undergoing intraarticular steroid injections and from orthopedic control patients. Gene expression profiles of the JIA and control FLS were obtained using the Affymetrix platform, with application of Ingenuity Pathway Analysis and Gene Set Enrichment Analysis software to define gene sets in dysregulated pathways and networks of potential pathologic relevance in this disease. Biologically relevant differentially expressed genes were confirmed by RNA and protein analysis. RESULTS: Exploration of global gene expression profiles of the JIA FLS revealed important dysregulated pathways, including the transforming growth factor ß (TGFß) signaling, as well as endochondral bone formation, cartilage formation, and ß-catenin networks. Importantly, bone morphogenetic protein 4 (BMP-4) was significantly overexpressed in the JIA FLS. FLS from patients with oligoarticular JIA exhibit a chondrocyte phenotype, as evidenced by expression of type II collagen and aggrecan. CONCLUSION: Dysregulation of the pathways involved in the pathogenesis of oligoarticular JIA were revealed through gene expression profiling. JIA FLS displayed dysregulated TGFß signaling and exhibited a hypertrophic chondrocyte phenotype. These characteristics, along with contributions from the ß-catenin network may have implications for endochondral bone formation and local growth disturbances in oligoarticular JIA. Overexpression of BMP-4 in FLS from patients with oligoarticular JIA in particular may play an important role in disease pathogenesis, with a direct effect on functional outcome and with implications for future treatment.


Asunto(s)
Artritis Juvenil/metabolismo , Proteína Morfogenética Ósea 4/metabolismo , Condrocitos/patología , Hiperostosis/metabolismo , Fenotipo , Transducción de Señal/fisiología , Membrana Sinovial/metabolismo , Factor de Crecimiento Transformador beta/metabolismo , Adolescente , Agrecanos/genética , Agrecanos/metabolismo , Artritis Juvenil/patología , Artritis Juvenil/fisiopatología , Proteína Morfogenética Ósea 4/genética , Estudios de Casos y Controles , Diferenciación Celular/fisiología , Niño , Preescolar , Condrocitos/metabolismo , Colágeno Tipo II/genética , Colágeno Tipo II/metabolismo , Femenino , Perfilación de la Expresión Génica , Humanos , Hiperostosis/patología , Hiperostosis/fisiopatología , Masculino , Osteogénesis/fisiología , Líquido Sinovial/metabolismo , Membrana Sinovial/patología , Factor de Crecimiento Transformador beta/genética , beta Catenina/genética , beta Catenina/metabolismo
10.
Pediatrics ; 131(6): e1977-81, 2013 Jun.
Artículo en Inglés | MEDLINE | ID: mdl-23669522

RESUMEN

Erythema of the ear lobe in the context of Lyme disease is caused by either borrelial lymphocytoma or localized erythema migrans. Here we present a case of chondritis limited to the ear cartilage caused by Lyme disease. The patient was treated with ceftriaxone with complete resolution of symptoms.


Asunto(s)
Borrelia/efectos de los fármacos , Enfermedades de los Cartílagos/diagnóstico , Cartílago Auricular/patología , Eritema Crónico Migrans/diagnóstico , Antibacterianos/uso terapéutico , Enfermedades de los Cartílagos/microbiología , Ceftriaxona/uso terapéutico , Niño , Diagnóstico Diferencial , Cartílago Auricular/microbiología , Eritema Crónico Migrans/tratamiento farmacológico , Eritema Crónico Migrans/etiología , Femenino , Humanos
12.
Clin Rheumatol ; 28(5): 591-3, 2009 May.
Artículo en Inglés | MEDLINE | ID: mdl-19194735

RESUMEN

Ten percent of Lyme arthritis (LA) patients have continued synovitis despite antimicrobial therapy. The current study was designed to (1) investigate predictors of prolonged disease and (2) further define natural history of pediatric LA. Medical records of 94 children fulfilling Centers for Disease Control criteria for Lyme disease were reviewed, classified into groups according to duration of synovitis, and SPSS statistical software was used for analysis. Thirty-nine percent required >6 months and 13% required >12 months to resolve LA. Pearson correlation between duration of symptoms of LA pretreatment and duration of synovitis was not significant. When patients were stratified by group, no differences were found for age, antinuclear antibodies positivity, enzyme-linked immunosorbent assay titer, or reactivity of Western blot using parametric and nonparametric tests. Linear and logistic regression showed no predictors of disease duration. One third of pediatric LA patients require >6 months to resolve synovitis. Duration is not associated with delay in treatment, age, or seroreactivity.


Asunto(s)
Enfermedad de Lyme/complicaciones , Enfermedad de Lyme/diagnóstico , Sinovitis/complicaciones , Sinovitis/diagnóstico , Infecciones por Borrelia/complicaciones , Infecciones por Borrelia/diagnóstico , Niño , Ensayo de Inmunoadsorción Enzimática/métodos , Femenino , Humanos , Masculino , Modelos Estadísticos , Análisis de Regresión , Programas Informáticos , Factores de Tiempo , Resultado del Tratamiento
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