RESUMEN
PURPOSE: The aim of this study was to determine serum and aqueous xanthine oxidase (XO) levels, and mRNA expression in anterior lens epithelial cells in pseudoexfoliation (PEX). METHODS: In this prospective study, serum, aqueous and anterior lens capsules were taken from 21 patients with PEX and 23 normal subjects who had undergone routine cataract surgery. Serum and aqueous XO levels were analyzed using the colorimetric method. mRNA expression of XO in anterior lens epithelial cells was evaluated using reverse transcription polymerase chain reaction analysis. RESULTS: Serum XO levels (means ± standard deviations) were 207.0 ± 86.1 IU/mL and 240.6 ± 114.1 IU/mL in the normal and PEX groups, respectively (p = 0.310). Aqueous XO levels (means ± standard deviations) were 65.5 ± 54.3 IU/mL in the normal group and 130.5 ± 117.4 IU/mL in the PEX group (p = 0.028). There was a 2.9 fold decrease in mRNA expression in anterior lens epithelial cells of PEX, which is significantly lower than the normal group (p = 0.01). CONCLUSIONS: Higher aqueous XO levels lacking associated different serum XO suggests higher oxidative stress in the aqueous. Higher aqueous XO levels in PEX with decreased mRNA expression in anterior lens epithelial cells indicate possible overexpression of XO in other structures related to the aqueous.
Asunto(s)
Humor Acuoso/enzimología , Células Epiteliales/enzimología , Síndrome de Exfoliación/genética , Regulación Enzimológica de la Expresión Génica/fisiología , ARN Mensajero/genética , Xantina Oxidasa/sangre , Xantina Oxidasa/genética , Anciano , Anciano de 80 o más Años , Cápsula Anterior del Cristalino/citología , Síndrome de Exfoliación/enzimología , Femenino , Humanos , Presión Intraocular , Masculino , Estudios Prospectivos , Reacción en Cadena en Tiempo Real de la Polimerasa , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Agudeza Visual/fisiologíaRESUMEN
PURPOSE: Omentin, a member of the adipocytokines family, is derived from adipose tissue and a lower level of serum omentin is considered as a metabolic risk factor. The aim of the present study is to evaluate the serum levels of omentin in patients with pseudoexfoliation syndrome (PES). MATERIALS AND METHODS: Patients without any systemic or ocular disease other than PES were included in the study. Age-matched and sex-matched healthy volunteers without PES were accepted as a control group. After detailed ophthalmologic examination, blood samples were obtained from a forearm vein. Serum levels of omentin were determined by the method of enzyme-linked immunosorbent assay. RESULTS: The mean age of the PES group (12 females, 12 males, n=24) was 75.2 ± 8.4 years, and the control group (10 females, 10 males, n=20) was 75 ± 6.7 years. There was no difference between the groups in terms of age (P=0.93) and sex (P=0.9). The mean serum levels of omentin in the PES group were 801.5 ± 317.1 ng/mL and in the control group were 1150.1 ± 584.1 ng/mL. The mean serum omentin levels were significantly lower in patients with PES (P=0.016). CONCLUSION: Lower levels of serum omentin in patients with PES compared with healthy subjects may support the theory of systemic nature of the disease.
Asunto(s)
Citocinas/sangre , Síndrome de Exfoliación/sangre , Lectinas/sangre , Anciano , Presión Sanguínea , Ensayo de Inmunoadsorción Enzimática , Femenino , Proteínas Ligadas a GPI/sangre , Humanos , Presión Intraocular , Masculino , Estudios Prospectivos , Factores de Riesgo , Tonometría OcularRESUMEN
A 27-year-old man who presented with diplopia and proptosis and was diagnosed with euthyroid Graves disease and restrictive strabismus. It was determined that his symptoms were caused by isolated lateral rectus muscle involvement. Diplopia resolved and ocular motility improved substantially following 6 weeks of oral corticosteroid therapy.