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High pressures may strongly affect the mesoscopic structure of some ionic liquids. In particular, the so called sponge-like structure is gradually destroyed when an increasing pressure is applied. Here we show how a polar solute, an apolar solute or a mixture thereof behave in the ionic liquid trihexyl, tetradecylphosphonium bis (trifluoromethyl-sulfonyl) imide when the pressure is raised up to 10 kbar. Our calculations clearly show an association between molecules that would not interact in ordinary conditions.
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It has been recently shown that pressure may affect the mesoscopic heterogeneity in aprotic ionic liquids, owing to the long alkyl chain folding on itself. Here we explore protic ionic liquids, using classical molecular dynamics. These compounds have shorter and stiffer alkyl chains, harder to fold. We observed that high pressure affects the mesoscopic structure of the studied chemicals and, indeed, the effect may be ascribed to chain folding.
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In this work we report an analysis of the bulk phase of 2-methoxyethylammonium nitrate based on ab initio molecular dynamics. The structural and dynamical features of the ionic liquid have been characterized and the computational findings have been compared with the experimental X-ray diffraction patterns, with infrared spectroscopy data, and with the results obtained from molecular dynamics simulations. The experimental infrared spectrum was interpreted with the support of calculated vibrational density of states as well as harmonic frequency calculations of selected gas phase clusters. Particular attention was addressed to the high frequency region of the cation (ω > 2000 cm(-1)), where the vibrational motions involve the NH3+ group responsible for hydrogen bond formation, and to the frequency range 1200-1400 cm(-1) where the antisymmetric stretching mode (ν3) of nitrate is found. Its multiple absorption lines in the liquid arise from the removal of the degeneracy present in the D3h symmetry of the isolated ion. Our ab initio molecular dynamics leads to a rationalization of the frequency shifts and splittings, which are inextricably related to the structural modifications induced by a hydrogen bonding environment. The DFT calculations lead to an inhomogeneous environment.
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The combination of amino acid anions with the choline cation gives origin to a new and potentially important class of organic ionic liquids that might represent a viable and bio-compatible alternative with respect to the traditional ones. We present here a detailed study of the bulk phase of the prototype system composed of the simplest amino acid (alanine) anion and the choline cation, based on ab initio and classical molecular dynamics. Theoretical findings have been validated by comparing with accurate experimental X-ray diffraction data and infrared spectra. We find that hydrogen bonding (HB) features in these systems are crucial in establishing their local geometric structure. We have also found that these HBs once formed are persistent and that the proton resides exclusively on the choline cation. In addition, we show that a classical force field description for this particular ionic liquid can be accurately performed by using a slightly modified version of the generalized AMBER force field.
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A combined approach of molecular dynamics simulations, wide angle X-ray scattering experiments, and density measurements was employed to study the structural properties of N-methyl-2-pyrrolidone (NMP) + water mixtures over the whole concentration range. Remarkably, a very good agreement between computed and experimental densities and diffraction patterns was achieved, especially if the effect of the mixture composition on NMP charges is taken into account. Analysis of the intermolecular organization, as revealed by the radial and spatial distribution functions of relevant solvent atoms, nicely explained the density maximum observed experimentally.
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We present an analysis of the liquid and of a small isolated cluster of n-ethyl ammonium nitrate based on "first principles" molecular dynamics. We discover that the peculiar properties of ionic liquids make such compounds ideal candidates for such an analysis. We have been able to characterize some important features of the liquid structure and we have validated our simulations by comparing our findings with experimental vibrational spectra of the liquid phase. Theoretical spectra, which present a remarkable agreement with the measurements, besides the assignment of the main spectra features, allow an interpretation of the spectra at high frequencies where the vibrational motions involve the hydrogen-bonded atoms, thus providing a picture of the hydrogen bonding network that exists in such compounds.
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Classical neurophysiology suggests that the motor cortex (MI) has a unique role in action control. In contrast, this review presents evidence for multiple parieto-frontal spinal command modules that can bypass MI. Five observations support this modular perspective: (i) the statistics of cortical connectivity demonstrate functionally-related clusters of cortical areas, defining functional modules in the premotor, cingulate, and parietal cortices; (ii) different corticospinal pathways originate from the above areas, each with a distinct range of conduction velocities; (iii) the activation time of each module varies depending on task, and different modules can be activated simultaneously; (iv) a modular architecture with direct motor output is faster and less metabolically expensive than an architecture that relies on MI, given the slow connections between MI and other cortical areas; (v) lesions of the areas composing parieto-frontal modules have different effects from lesions of MI. Here we provide examples of six cortico-spinal modules and functions they subserve: module 1) arm reaching, tool use and object construction; module 2) spatial navigation and locomotion; module 3) grasping and observation of hand and mouth actions; module 4) action initiation, motor sequences, time encoding; module 5) conditional motor association and learning, action plan switching and action inhibition; module 6) planning defensive actions. These modules can serve as a library of tools to be recombined when faced with novel tasks, and MI might serve as a recombinatory hub. In conclusion, the availability of locally-stored information and multiple outflow paths supports the physiological plausibility of the proposed modular perspective.
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Mano , Lóbulo Parietal , Humanos , Lóbulo Parietal/fisiología , CogniciónRESUMEN
Laparoscopic cholecystectomy is the Gold Standard for the treatment of symptomatic cholelithiasis. There is still an increase in the rate of incidence of biliary and vascular injuries with laparoscopy. Hepatic artery pseudoaneurysm is a rare but serious complication associated with laparoscopic cholecystectomy and bile duct injury. The diagnosis may be difficult. Our experience of a case of iatrogenic lesion of the right hepatic artery with the formation of pseudoaneurysm treated by means of embolization of the artery is presented here.
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Aneurisma Falso/etiología , Colecistectomía Laparoscópica/efectos adversos , Colelitiasis/cirugía , Hemobilia/etiología , Arteria Hepática/lesiones , Enfermedad Iatrogénica , Aneurisma Falso/diagnóstico , Aneurisma Falso/terapia , Embolización Terapéutica , Femenino , Hemobilia/diagnóstico , Hemobilia/terapia , Humanos , Persona de Mediana Edad , Enfermedades Raras , Resultado del TratamientoRESUMEN
In this work we report molecular mechanics and ab initio calculations on the geminal di-imidazolium bis(trifluoromethylsulfonyl)amide ionic liquid in the gas phase. We report the likely energetically preferred geometries of the ionic complex and its main features in terms of charge distribution, electronic density, structure, and energetics. We find that the gas phase structure of the ionic complex is quite compact and that the alkyl chain connecting the two imidazolium charged rings is strongly bent in order to maximize their electrostatic interactions with the two anions.
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The authors want to present five cases (from May 1999 to May 2009) of acute abdomen from perforation of the foreign body introduced with food. They highlight how the accidental ingestion is very common but the perforation is rare. The preoperative diagnosis, in these cases, is always very difficult and the radiological examinations are not always able to resolve the diagnostic doubt with other acute intestinal diseases that are responsible of perforation. Then, the surgery procedure is, necessarily, the only possible diagnostic and therapeutic means. The mortality and the morbidity remain still high first of all for the delay in the diagnosis and the advanced age of patients.
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Ciego/lesiones , Colon Sigmoide/lesiones , Migración de Cuerpo Extraño/complicaciones , Migración de Cuerpo Extraño/cirugía , Íleon/lesiones , Perforación Intestinal/etiología , Perforación Intestinal/cirugía , Abdomen Agudo/etiología , Anciano , Anciano de 80 o más Años , Femenino , Migración de Cuerpo Extraño/diagnóstico , Humanos , Perforación Intestinal/diagnóstico , Masculino , Persona de Mediana Edad , Estudios Retrospectivos , Resultado del TratamientoRESUMEN
BACKGROUND: The prognosis of patients with peritoneal tumors has been improved by the association of cytoreductive surgery with hyperthermic intraperitoneal chemotherapy, though still with an unclear impact on patients' quality of life. The purpose of our study was to evaluate the quality of life in 18 cases submitted to cytoreductive surgery and hyperthermic intraperitoneal chemotherapy and particularly to identify the factors that influence it. PATIENTS AND METHODS: Quality of life was evaluated using the functional assessment of cancer therapy; the results were correlated with 25 parameters. RESULTS: The study demonstrated that the patients'quality of life was not modified by treatment with cytoreductive surgery and hyperthermic intraperitoneal chemotherapy; the dose of mitomycin C, the site of the primary tumor, gastrointestinal, renal and neurological toxicity, adjuvant chemotherapy, the patients' age and leukopenia were factors that influenced the quality of life. CONCLUSION: Cytoreductive surgery with hyperthermic intraperitoneal chemotherapy allows conservation of preoperative quality of life.
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Neoplasias del Colon/terapia , Hipertermia Inducida , Neoplasias Ováricas/terapia , Neoplasias Peritoneales/terapia , Calidad de Vida , Neoplasias Gástricas/terapia , Adulto , Anciano , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Carboplatino/administración & dosificación , Quimioterapia Adyuvante , Neoplasias del Colon/patología , Neoplasias del Colon/psicología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Mitomicina/uso terapéutico , Neoplasias Ováricas/patología , Neoplasias Ováricas/psicología , Paclitaxel/administración & dosificación , Cavidad Peritoneal/patología , Neoplasias Peritoneales/psicología , Neoplasias Peritoneales/secundario , Pronóstico , Neoplasias Gástricas/patología , Neoplasias Gástricas/psicología , Encuestas y CuestionariosRESUMEN
In the present study, we used tumour necrosis factor-alpha receptor 1 knock-out mice (TNF-alphaR1KO) to evaluate an in vivo role of TNF-alphaR1 on the pathogenesis of inflammatory diseases. We used a murine model of carrageenan-induced acute inflammation (pleurisy), a preclinical model of airway inflammation. The data proved that TNF-alphaR1KO were resistant to carrageenan-induced acute inflammation compared with TNF-alpha wild-type mice. TNF-alphaR1KO showed a significant reduction in accumulation of pleural exudate and in the number of inflammatory cells, in lung infiltration of polymorphonuclear leucocytes and lipid peroxidation and showed a decreased production of nitrite/nitrate in pleural exudates. Furthermore, the intensity and degree of the adhesion molecule intercellular adhesion molecule-1 and P-selectin, Fas ligand (FasL), inducible nitric oxide sythase and nitrotyrosine determined by immunohistochemical analysis were reduced markedly in lung tissues from TNF-alphaR1KO at 4 h and 24 h after carrageenan injection. Moreover, TNF-alpha and interleukin-1beta concentrations were reduced in inflamed areas and in pleural exudates from TNF-alphaR1KO. To support the results generated using pleural inflammation, carrageenan-induced paw oedema models were also performed. In order to elucidate whether the observed anti-inflammatory effects were related to the inhibition of TNF-alpha, we also investigated the effect of etanercept, a TNF-alpha soluble receptor construct, on carrageenan-induced pleurisy. The treatment with etanercept (5 mg/kg subcutaneously 2 h before the carrageenan injection) reduces markedly both laboratory and histological signs of carrageenan-induced pleurisy. Our results showed that administration of etanercept resulted in the same outcome as that of deletion of the TNF-alphaR1 receptor, adding a new insight to TNF-alpha as an excellent target by therapeutic applications.
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Pleuresia/inmunología , Receptores Tipo I de Factores de Necrosis Tumoral/genética , Enfermedad Aguda , Animales , Biomarcadores/análisis , Carragenina , Edema/tratamiento farmacológico , Edema/inmunología , Etanercept , Proteína Ligando Fas/análisis , Eliminación de Gen , Miembro Posterior , Inmunoglobulina G/uso terapéutico , Inmunohistoquímica , Molécula 1 de Adhesión Intercelular/análisis , Interleucina-1/análisis , Pulmón/inmunología , Pulmón/patología , Masculino , Ratones , Ratones Noqueados , Modelos Animales , Selectina-P/análisis , Pleuresia/tratamiento farmacológico , Pleuresia/patología , Receptores del Factor de Necrosis Tumoral/uso terapéutico , Sustancias Reactivas al Ácido Tiobarbitúrico/análisis , Factor de Necrosis Tumoral alfa/análisis , Factor de Necrosis Tumoral alfa/antagonistas & inhibidores , Tirosina/análogos & derivados , Tirosina/análisisRESUMEN
BACKGROUND AND PURPOSE: 5-lipoxygenase (5-LO) is the key enzyme in leukotriene (LT) biosynthesis from arachidonic acid (AA). Here, we examined the role of the 5-LO-product, cysteinyl-LT (Cys-LT), with a 5-LO inhibitor (zileuton) and a Cys-LT, receptor antagonist (montelukast), in the inflammatory response and tissue injury associated with spinal cord injury (SCI). EXPERIMENTAL APPROACH: SCI was induced in mice by the application of vascular clips to the dura via a two-level T6 to T7 laminectomy for 1 min. Cord inflammation was assessed histologically and by measuring inflammatory mediators (ELISA) and apoptosis by annexin V, TUNEL, Fas ligand staining and Bax and Bcl-2 expression (immunohistochemistry and western blots). Motor function in hindlimbs was assessed by a locomotor rating scale, for 10 days after cord injury. KEY RESULTS: SCI in mice resulted in tissue damage, oedema, neutrophil infiltration, apoptosis, tumour necrosis-alpha (TNF-alpha) and cyclooxygenase-2 (COX-2) expression, prostaglandin E(2) (PGE(2)) and leukotriene B(4) (LTB(4)) production, and extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation in injured tissue. Treatment of the mice with zileuton or montelukast reduced the spinal cord inflammation and tissue injury, neutrophil infiltration, TNF-alpha, COX-2 and pERK1/2 expression, PGE(2) and LTB(4) production, and apoptosis. In separate experiments, zileuton or montelukast significantly improved the recovery of limb function over 10 days. CONCLUSIONS AND IMPLICATIONS: Zileuton and montelukast produced a substantial reduction of inflammatory events associated with experimental SCI. Our data underline the important role of 5-LO and Cys-LT in neurotrauma.
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Acetatos/farmacología , Hidroxiurea/análogos & derivados , Inhibidores de la Lipooxigenasa/farmacología , Quinolinas/farmacología , Traumatismos de la Médula Espinal/tratamiento farmacológico , Animales , Apoptosis/efectos de los fármacos , Araquidonato 5-Lipooxigenasa/efectos de los fármacos , Araquidonato 5-Lipooxigenasa/metabolismo , Ciclooxigenasa 2/efectos de los fármacos , Ciclooxigenasa 2/metabolismo , Ciclopropanos , Cisteína/efectos de los fármacos , Cisteína/metabolismo , Dinoprostona/biosíntesis , Modelos Animales de Enfermedad , Regulación de la Expresión Génica/efectos de los fármacos , Hidroxiurea/farmacología , Inflamación/tratamiento farmacológico , Inflamación/etiología , Leucotrienos/metabolismo , Masculino , Ratones , Infiltración Neutrófila/efectos de los fármacos , Recuperación de la Función/efectos de los fármacos , Traumatismos de la Médula Espinal/fisiopatología , Sulfuros , Factor de Necrosis Tumoral alfa/efectos de los fármacos , Factor de Necrosis Tumoral alfa/metabolismoRESUMEN
Main purpose of the work is assembling, testing and optimizing new disposable amperometric biosensors to analyze substances in different application fields as agribusiness, clinical chemistry and environment protection. Many kinds of modified electrodes have been prepared and tested to build portable devices to analyze quickly many analytes, in a simple and cost-effective manner. Bare electrodes of the screen-printed type, with silver as reference, have been used for modification. The glassy carbon electrodes with multi-walled carbon nanotubes or graphene or gold nanoparticles depositions were modified with generation IV ionic liquids. Choline as cation and amino acids, such as glycine, serine, phenylalanine and histidine, as anions have been employed for these ionic liquids. The presence of nanostructured materials on the electrode brings an increased contact surface between analytes and receptor and, consequently, an amplification of the amperometric signal and a better sensibility. Moreover the use of new ionic liquids of generation IV, biologically friendly and water soluble, improves the electronic transfer, facilitating and strengthening the redox reaction nearby the electrode. By immobilizing the proper enzymes onto the modified electrode surface, different compounds of analytical interest can be determined by means of sensitive, properly designed amperometric biosensors. Analytes such as antioxidant components in extra-virgin olive oils, alcohols in beverages and glucose in food matrices have been tested, using a suitable enzyme: microbial lipase, alcohol dehydrogenase and glucose oxidase, respectively.
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Técnicas Biosensibles/instrumentación , Enzimas Inmovilizadas/química , Líquidos Iónicos/química , Nanoestructuras/química , Alcohol Deshidrogenasa/química , Animales , Aspergillus niger/enzimología , Candida/enzimología , Electrodos , Etanol/análisis , Glucosa/análisis , Glucosa Oxidasa/química , Oro/química , Lipasa/química , Nanopartículas del Metal/química , Nanopartículas del Metal/ultraestructura , Modelos Moleculares , Nanoestructuras/ultraestructura , Nanotubos de Carbono/química , Nanotubos de Carbono/ultraestructura , Aceite de Oliva/análisis , Porcinos , TemperaturaRESUMEN
The ability to act jointly with others is a hallmark of primate evolution and is fundamental for human development. In recent years, the study of coordination strategies between individuals performing joint actions has received growing attention. However, when, in the course of post-natal development, this cognitive-motor function emerges is still unknown. Here, we studied dyads of peers aged 6-9 years, as well as adult subjects, while they performed a task where the same action, namely, exerting hand force on an isometric joystick to move a visual cursor from a central toward a peripheral target, was performed in a "solo" and in a social "cooperative" context. The results revealed that during joint action planning, an attempt to synchronize one's own action with that of a partner emerges at 7 years of age, together with a reduction in the duration and variability of the reaction times. A critical time is 8 years, when "solo" performance reaches a high level of accuracy. From this age, another coordination strategy, based on the online monitoring of the peer's behavior, seems to be implemented during the execution of joint action. The motor and cognitive development occurring during childhood are discussed as possible mechanisms mediating, respectively, the capability and the propensity to take into account the peer's behavior for implementing a common action plan.
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Atención/fisiología , Desarrollo Infantil/fisiología , Cognición/fisiología , Conducta Cooperativa , Desempeño Psicomotor/fisiología , Factores de Edad , Niño , Femenino , Humanos , Relaciones Interpersonales , Masculino , Actividad Motora , Sistemas en LíneaRESUMEN
Recent studies of visually guided reaching in monkeys support the hypothesis that the visuomotor transformations underlying arm movements to spatial targets involve a parallel mechanism that simultaneously engages functionally related frontal and parietal areas linked by reciprocal cortico-cortical connections. The neurons in these areas possess similar combinations of response properties. The multimodal combinatorial properties of these neurons and the gradient architecture of the parietofrontal network emerge as a potential substrate to link the different sensory and motor signals that arise during reaching behavior into common hybrid reference frames. This convergent combinatorial process is evident at early stages of visual information processing in the occipito-parietal cortex, suggesting the existence of re-entrant motor influences on cortical areas once believed to have only visual functions.
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Corteza Cerebral/fisiología , Desempeño Psicomotor/fisiología , Animales , Lóbulo Frontal/fisiología , Movimiento/fisiología , Red Nerviosa/fisiología , Lóbulo Parietal/fisiología , Factores de TiempoRESUMEN
BACKGROUND: Mini-invasive techniques have revolutionized surgery, but the superiority of laparoscopic access for appendectomy is widely debated. The authors analyze their monocentric experience with 1,347 laparoscopic appendectomies. METHODS: Between October 1991 and December 2002, all the patients with an indication for appendectomy underwent surgery (301 emergency and 1,046 interval appendectomies) using the laparoscopic approach. RESULTS: For 1,248 patients, appendectomy was performed laparoscopically, whereas for 99 patients (7.3%), it was converted to an open procedure because of technical reasons (90 patients, 6.7%) or intraoperative complications (9 patients, 0.6%). For 59 patients (4.4%), the appendectomy was associated with another procedure. Histology showed "acute" alterations in 261 of the 301 emergency surgeries and in 148 of the 1,046 elective operations. Postoperative complications arose in 37 patients (2.7%), with 5 patients (0.3%) requiring invasive treatment. The mean postoperative stay was 30 h. CONCLUSIONS: Laparoscopic appendectomy offers unquestionable advantages, but it is not yet considered the "gold standard" for appendiceal pathology. Many centers reserve it for selected patients (e.g., obese patients and women suspected of having other pathologies). No randomized trials or metaanalyses have definitively proved its superiority.
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Apendicectomía , Laparoscopía , Cirugía Asistida por Video , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Niño , Femenino , Humanos , Masculino , Persona de Mediana Edad , Complicaciones PosoperatoriasRESUMEN
Calcium phosphate bone cements (CPCs) with antibacterial properties are demanded for clinical applications. In this study, we demonstrated the use of a relatively simple processing route based on preparation of silver-doped CPCs (CPCs-Ag) through the preparation of solid dispersed active powder phase. Real-time monitoring of structural transformations and kinetics of several CPCs-Ag formulations (Ag = 0 wt %, 0.6 wt % and 1.0 wt %) was performed by the Energy Dispersive X-ray Diffraction technique. The partial conversion of ß-tricalcium phosphate (TCP) phase into the dicalcium phosphate dihydrate (DCPD) took place in all the investigated cement systems. In the pristine cement powders, Ag in its metallic form was found, whereas for CPC-Ag 0.6 wt % and CPC-Ag 1.0 wt % cements, CaAg(PO3)3 was detected and Ag (met.) was no longer present. The CPC-Ag 0 wt % cement exhibited a compressive strength of 6.5 ± 1.0 MPa, whereas for the doped cements (CPC-Ag 0.6 wt % and CPC-Ag 1.0 wt %) the reduced values of the compressive strength 4.0 ± 1.0 and 1.5 ± 1.0 MPa, respectively, were detected. Silver-ion release from CPC-Ag 0.6 wt % and CPC-Ag 1.0 wt % cements, measured by the Atomic Emission Spectroscopy, corresponds to the average values of 25 µg/L and 43 µg/L, respectively, rising a plateau after 15 days. The results of the antibacterial test proved the inhibitory effect towards pathogenic Escherichia coli for both CPC-Ag 0.6 wt % and CPC-Ag 1.0 wt % cements, better performances being observed for the cement with a higher Ag-content.
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The ability of human and non-human primates to make fast corrections to hand movement trajectories after a sudden shift in the target's location is a key feature of visuo-motor behavior. In healthy individuals, hand movements smoothly adapt to a change in target location without needing to complete the movement to the first target location, as typical of parietal patients. This finding indicates that the nervous system continuously monitors the visual scene and is able to integrate new information in order to produce an efficient motor response. In this paper, we review the kinematics, reaction times and muscle activity observed during the online correction of hand movements as well as the underlying neurophysiological processes studied through single-cell neural recordings in monkeys. Brain stimulation, lesion and imaging studies in humans are also discussed. We demonstrate that while online correction mechanisms strongly depend on the activity of a parieto-frontal network of which the posterior parietal cortex is a crucial node, these mechanisms proceed smoothly and are similar to what is observed during simple point-to-point movements. Online correction of hand movements would rely on feedforward and feedback mechanisms in the parietal cortex, as part of the activity within the fronto-parietal network for the planning and execution of visuo-motor tasks.
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Mano/fisiología , Corteza Motora/fisiología , Movimiento/fisiología , Lóbulo Parietal/fisiología , Percepción Visual/fisiología , Retroalimentación Sensorial/fisiología , Humanos , Intención , Neuronas/fisiología , Desempeño Psicomotor/fisiología , Tiempo de ReacciónRESUMEN
The callosal connections of the superior parietal lobule, area 5 of Brodmann, were studied in macaque monkeys (M. nemestrina and M. fascicularis) using anatomical techniques based on both anterograde and retrograde axoplasmic transport of wheat-germ-agglutinin-conjugated horseradish peroxidase. From sagittal sections, two-dimensional flattened computer reconstructions of the volumes of cortical tissue containing callosal-projecting neurons (callosal efferent zone) and/or callosal terminal axons (callosal terminal territory) were obtained. Callosal zones were found in area 5, including the supplementary sensory area, in a limited part of area 6, i.e., in the supplementary motor area, in area 7b, in the cortex of the dorsal bank of the sylvian fissure, and in a limited part of area 7a, in the cortex of the upper third of the rostral bank of the superior temporal sulcus. Callosal neurons in all cortical areas studied, though with regional variations, predominated in layer IIIb, but were also very numerous in layers VI and V. They were rare in other cortical laminae. In the cortical regions projecting heterotopically to area 5, the tangential distribution of callosal neurons was discontinuous because of the presence of large acallosal regions. These were not observed in area 5, although here the distribution of callosal neurons waxed and waned in the tangential cortical plane. Callosal axons to and/or from area 5 crossed the midline in the posterior, presplenial part of the corpus callosum. In the superior parietal lobule they terminated in radial patches or columns, spanning layers I-IV. These columns of various width (200-2,000 micron) were separated by gaps of similar size, free of such terminals. Callosal neurons were present not only within, but also between, the callosal terminal columns. Callosal neurons located within the callosal terminal columns were, in a statistically significant way, more numerous than those located between them. The callosal efferent zone occupied 71% of the tangential domain of area 5, whereas the callosal terminal territory occupied only 49% of it. This difference is statistically significant. The discontinuous columnar arrangement of callosal terminals and the periodic distribution of callosal neurons in the lateral part of area 5 defined three main bands of callosal connections of irregular shape which were oriented mediolaterally and ran parallel to the main architectonic borders, the border between areas 2 and 5 and that between 5 and 7.