Circ_0000745 strengthens the expression of CCND1 by functioning as miR-488 sponge and interacting with HuR binding protein to facilitate the development of oral squamous cell carcinoma.

BACKGROUND: The implication of circular RNAs (circRNAs) in human cancers has aroused much concern. In this study, we investigated the function of circ_0000745 and its potential functional mechanisms in oral squamous cell carcinoma (OSCC) to further understand OSCC pathogenesis. METHODS: The expression of circ_0000745, miR-488 and cyclin D1 (CCND1) mRNA was measured by quantitative real-time polymerase chain reaction (qPCR). Cell proliferation capacity was assessed by cell counting kit-8 (CCK-8) assay and colony formation assay. Cell cycle progression and cell apoptosis were determined by flow cytometry assay. The protein levels of CCND1, PCNA, Cleaved-caspase 3 and HuR were detected by western blot. Animal study was conducted to identify the role of circ_0000745 in vivo. The targeted relationship was verified by dual-luciferase reporter assay, pull-down assay or RNA immunoprecipitation (RIP) assay. RESULTS: The expression of circ_0000745 was increased in OSCC tissues and cells. Circ_0000745 downregulation inhibited OSCC cell proliferation and induced cell cycle arrest and apoptosis in vitro, as well as blocked tumor growth in vivo. MiR-488 was a target of circ_0000745, and circ_0000745 downregulation suppressed OSCC development by enriching miR-488. Besides, circ_0000745 regulated CCND1 expression by targeting miR-488. In addition, circ_0000745 regulated CCND1 expression by interacting with HuR protein. CCND1 knockdown also inhibited OSCC cell proliferation and induced cell cycle arrest and apoptosis in vitro, and CCND1 overexpression recovered the inhibitory effects on OSCC cell malignant behaviors caused by circ_0000745 downregulation. CONCLUSIONS: Circ_0000745 regulated the expression of CCND1 partly by acting as miR-488 sponge and interacting with HuR protein, thus promoting the progression of OSCC.

[Silencing MSH3 expression enhances cisplatin sensitivity of human tongue cancer cells].

OBJECTIVE: To explore the effect of MSH3 knock-down on sensitivity of tongue cancer cells to cisplatin. METHODS: Three small interfering RNA (siRNA) fragments targeting MSH3 CDS region were synthesized and transfected into CAL27 cells via Lipofectamine. Real-time PCR and Western blotting were used to assess the efficiency of MSH3 silencing. MTS, apoptosis staining and cell immunofluorescence assay were used to examine the cisplatin sensitivity, apoptosis and DNA repair of transfected CAL27 cells. RESULTS: s One of the 3 siRNAs was found to significantly reduce the expression of MSH3 protein in CAL27 cells (P<0.05). MTS assay showed that MSH3 silencing resulted in an significant reduction of IC50 of cisplatin from 21.32 to 13.95 µmol/L (P<0.05) and increased the apoptotic index of the exposed cells from 4.23∓1.27 to 11.32∓1.82 (P<0.05). Immunofluorescence assay demonstrated that silencing MSH3 markedly reduced the number of γ-H2AX foci. CONCLUSION: Silencing MSH3 can significantly increase cisplatin sensitivity of tongue cancer cells, the mechanism of which involves mainly attenuation of repair of DNA double-strand damage in the cells.

[Investigation and analysis of depression occurrence in patients with chronic periodontitis].

PURPOSE: To investigate and analyze the depression of patients with chronic periodontitis. METHODS: The subjects were divided t into two groups-those with chronic periodontitis and those with healthy periodontal (control group). Each subject was evaluated by questionnaire, the value of each questionnaire with Depressive Experiences Questionnaire (DEQ) and Zung Self-rating Depression Scale (SDS) was obtained and the average value of each group's DEQ-A (dependent factor), DEQ-I (self-criticism factor) and SDS was calculated. SPSS13.0 software package was used to analyze the data. RESULTS: The average SDS value in the chronic periodontitis group was significantly higher than that in the control group, the average DEQ -A and DEQ-I value of chronic periodontitis group was also significantly higher. The average DEQ and SDS value in the severe chronic periodontitis group was significantly higher than that in the mild group(P < 0.05). CONCLUSIONS: Depression has some relationship with chronic periodontitis. DEQ-A,DEQ-I and SDS are significantly different between severe chronic periodontitis patients and mild chronic periodontitis patients; Compared with the control group,the scores of both the anaelitic depression and introjective depression factors in the chronic periodontitis group increase significantly. Supported by Research Fund of Department of Science and Technology of Hengyang City of Hunan Province(2009KS34).

[Clinical analysis of peripheral branch evulsion of trigeminal nerves on treatment of trigeminal neuralgia:report of 136 cases].

PURPOSE: To investigate the clinical effect of peripheral branch evulsion of trigeminal nerves on trigeminal neuralgia, especially on the aspect of odynolysis and recurrence. METHODS: 136 patients with trigeminal neuralgia underwent peripheral branch evulsion of trigeminal nerves were retrospectively reviewed with three years follow-up period. The odynolysis and recurrence of pain were observed. RESULTS: Excellent effect was obtained in one patient underwent branch I evulsion. Excellent, good, and no effect were obtained in 57.7% (45/78), 34.6% (27/78), and 7.7% (6/78) of patients underwent branch II evulsion, respectively, with the total effective rate of 92.3%. Excellent and good effect was obtained in 91.1% (82/91) and 8.9% (9/91) of patients underwent branch III evulsion, respectively, with the total effective rate of 100%. During the 3-year follow-up period, no pain recurrence occurred in the patient underwent branch I evulsion, pain recurrence occurred in 24 out of 68 patients underwent branch II evulsion, and in 3 out 72 patients underwent branch III evulsion. Furthermore, pain of branch II occurred in 6 patients underwent branch III evulsion, and pain of branch III occurred in 2 patients underwent branch II evulsion. CONCLUSIONS: The factors of pain recurrence and odynolysis are complicated. Clinically incomplete evulsion may be the main factor. Standard criteria are necessary for identification of pain recurrence and ectopic pain. Electrothermal treatment though infraorbital foramen, buccal nerve evulsion, and ligation of broken nerve are effective to treat odynolysis and pain recurrence.