RESUMEN
Considerable progress has been made toward elucidating the mechanism of Staphylococcus aureus aggregation in synovial fluid. In this study, aggregate morphology was assessed following incubation under several simulated postsurgical joint conditions. Using fluorescently labeled synovial fluid polymers, we show that aggregation occurs through two distinct mechanisms: (i) direct bridging between S. aureus cells and host fibrinogen and (ii) an entropy-driven depletion mechanism facilitated by hyaluronic acid and albumin. By screening surface adhesin-deficient mutants (clfA, clfB, fnbB, and fnbA), we identified the primary genetic determinant of aggregation in synovial fluid to be clumping factor A. To characterize this bridging interaction, we employed an atomic force microscopy-based approach to quantify the binding affinity of either wild-type S. aureus or the adhesin mutant to immobilized fibrinogen. Surprisingly, we found there to be cell-to-cell variability in the binding strength of the bacteria for immobilized fibrinogen. Superhigh-resolution microscopy imaging revealed that fibrinogen binding to the cell wall is heterogeneously distributed at both the single cell and population levels. Finally, we assessed the antibiotic tolerance of various aggregate morphologies arising from newly deciphered mechanisms of polymer-mediated synovial fluid-induced aggregation. The formation of macroscopic aggregates under shear was highly tolerant of gentamicin, while smaller aggregates, formed under static conditions, were susceptible. We hypothesize that aggregate formation in the joint cavity, in combination with shear, is mediated by both polymer-mediated aggregation mechanisms, with depletion forces enhancing the stability of essential bridging interactions. IMPORTANCE The formation of a bacterial biofilm in the postsurgical joint environment significantly complicates the resolution of an infection. To form a resilient biofilm, incoming bacteria must first survive the initial invasion of the joint space. We previously found that synovial fluid induces the formation of Staphylococcus aureus aggregates, which may provide rapid protection during the early stages of infection. The state of the host joint environment, including the presence of fluid flow and fluctuating abundance of synovial fluid polymers, determines the rate and size of aggregate formation. By expanding on our knowledge of the mechanism and pathogenic implications of synovial fluid-induced aggregation, we hope to contribute insights for the development of novel methods of prevention and therapeutic intervention.
Asunto(s)
Infecciones Estafilocócicas , Staphylococcus aureus , Humanos , Staphylococcus aureus/genética , Staphylococcus aureus/metabolismo , Antibacterianos/farmacología , Antibacterianos/metabolismo , Adhesinas Bacterianas/genética , Adhesinas Bacterianas/metabolismo , Biopelículas , Infecciones Estafilocócicas/microbiología , Fibrinógeno/metabolismo , Fibrinógeno/farmacologíaRESUMEN
Internal phosphorus (P) in sediments plays an important role in the nutrient dynamics of lakes, sometimes long after external loads have been reduced. Similarly, internal P sources may drive the nutrient dynamics of small agricultural streams that drain to larger rivers and lakes, despite best management practices intended to reduce external P loads from adjacent fields. Here, internal P concentrations were measured with sequential extraction on cores collected in spring and summer from two small agricultural streams in the drainage basin of Lake Erie, a large, eutrophic lake experiencing increasing SRP loads. Average total extractable P concentrations were similar to within 5% during spring and summer, but mobile P binding fractions nearly doubled in summer, possibly due to accelerated rates of organic matter mineralization or iron reduction beneath suboxic, stagnant surface waters. One site had chronically greater internal P concentrations by 25-75%, despite the implementation of best management practices such as grass buffers. The site also had more aquatic vegetation that restricted the flow, less dissolved oxygen in surface water, and greater organic matter in sediments during both seasons, suggesting that variations in hydrology, sediment composition, and vegetation influence hot spots of P retention throughout small agricultural streams.
Asunto(s)
Lagos , Ríos , Agricultura , Monitoreo del Ambiente , Sedimentos Geológicos , FósforoRESUMEN
The Staphylococcus aureus cell surface contains cell wall-anchored proteins such as fibronectin-binding protein A (FnBPA) that bind to host ligands (e.g. fibronectin; Fn) present in the extracellular matrix of tissue or coatings on cardiac implants. Recent clinical studies have found a correlation between cardiovascular infections caused by S. aureus and nonsynonymous SNPs in FnBPA. Atomic force microscopy (AFM), surface plasmon resonance (SPR), and molecular simulations were used to investigate interactions between Fn and each of eight 20-mer peptide variants containing amino acids Ala, Asn, Gln, His, Ile, and Lys at positions equivalent to 782 and/or 786 in Fn-binding repeat-9 of FnBPA. Experimentally measured bond lifetimes (1/koff) and dissociation constants (Kd = koff/kon), determined by mechanically dissociating the Fn·peptide complex at loading rates relevant to the cardiovascular system, varied from the lowest-affinity H782A/K786A peptide (0.011 s, 747 µm) to the highest-affinity H782Q/K786N peptide (0.192 s, 15.7 µm). These atomic force microscopy results tracked remarkably well to metadynamics simulations in which peptide detachment was defined solely by the free-energy landscape. Simulations and SPR experiments suggested that an Fn conformational change may enhance the stability of the binding complex for peptides with K786I or H782Q/K786I (Kdapp = 0.2-0.5 µm, as determined by SPR) compared with the lowest-affinity double-alanine peptide (Kdapp = 3.8 µm). Together, these findings demonstrate that amino acid substitutions in Fn-binding repeat-9 can significantly affect bond strength and influence the conformation of Fn upon binding. They provide a mechanistic explanation for the observation of nonsynonymous SNPs in fnbA among clinical isolates of S. aureus that cause endovascular infections.
Asunto(s)
Adhesinas Bacterianas/química , Adhesinas Bacterianas/genética , Polimorfismo de Nucleótido Simple , Staphylococcus aureus/química , Staphylococcus aureus/genética , Adhesinas Bacterianas/metabolismo , Sustitución de Aminoácidos , Microscopía de Fuerza Atómica , Mutación Missense , Secuencias Repetitivas de Aminoácido , Staphylococcus aureus/metabolismo , Resonancia por Plasmón de SuperficieRESUMEN
Endovascular infections caused by Staphylococcus aureus involve interactions with fibronectin present as extracellular matrix or surface ligand on host cells. We examined the expression, structure, and binding activity of the two major S. aureus fibronectin-binding proteins (FnBPA, FnBPB) in 10 distinct, methicillin-resistant clinical isolates from patients with either persistent or resolving bacteremia. The persistent bacteremia isolates (n = 5) formed significantly stronger bonds with immobilized fibronectin as determined by dynamic binding measurements performed with atomic force microscopy. Several notable differences were also observed when the results were grouped by clonal complex 5 (CC5) strains (n = 5) versus CC45 strains (n = 5). Fibronectin-binding receptors on CC5 formed stronger bonds with immobilized fibronectin (P < 0.001). The fnbA gene was expressed at higher levels in CC45, whereas fnbB was found in only CC5 isolates. The fnbB gene was not sequenced because all CC45 isolates lacked this gene. Instead, comparisons were made for fnbA, which was present in all 10 isolates. Sequencing of fnbA revealed discrete differences within high-affinity, fibronectin-binding repeats (FnBRs) of FnBPA that included (i) 5-amino-acid polymorphisms in FnBR-9, FnBR-10, and FnBR-11 involving charged or polar side chains, (ii) an extra, 38-amino-acid repeat inserted between FnBR-9 and FnBR-10 exclusively seen in CC45 isolates, and (iii) CC5 isolates had the SVDFEED epitope in FnBR-11 (a sequence shown to be essential for fibronectin binding), while this sequence was replaced in all CC45 isolates with GIDFVED (a motif known to favor host cell invasion at the cost of reduced fibronectin binding). These complementary sequence and binding data suggest that differences in fnbA and fnbB, particularly polymorphisms and duplications in FnBPA, give S. aureus two distinct advantages in human endovascular infections: (i) FnBPs similar to that of CC5 enhance ligand binding and foster initiation of disease, and (ii) CC45-like FnBPs promote cell invasion, a key attribute in persistent endovascular infections.
Asunto(s)
Adhesinas Bacterianas/genética , Bacteriemia/microbiología , Fibronectinas/metabolismo , Staphylococcus aureus Resistente a Meticilina/genética , Isoformas de Proteínas/genética , Infecciones Estafilocócicas/microbiología , Adhesinas Bacterianas/química , Adhesinas Bacterianas/metabolismo , Secuencia de Aminoácidos , Bacteriemia/patología , Sitios de Unión , Vasos Sanguíneos/microbiología , Vasos Sanguíneos/patología , Células Clonales , Fibronectinas/química , Expresión Génica , Interacciones Huésped-Patógeno , Humanos , Proteínas Inmovilizadas/química , Proteínas Inmovilizadas/metabolismo , Staphylococcus aureus Resistente a Meticilina/clasificación , Staphylococcus aureus Resistente a Meticilina/metabolismo , Staphylococcus aureus Resistente a Meticilina/patogenicidad , Datos de Secuencia Molecular , Polimorfismo Genético , Unión Proteica , Isoformas de Proteínas/química , Isoformas de Proteínas/metabolismo , Estructura Terciaria de Proteína , Infecciones Estafilocócicas/patologíaRESUMEN
Medical implants, like cardiovascular devices, improve the quality of life for countless individuals but may become infected with bacteria like Staphylococcus aureus. Such infections take the form of a biofilm, a structured community of bacterial cells adherent to the surface of a solid substrate. Every biofilm begins with an attractive force or bond between bacterium and substratum. We used atomic force microscopy to probe experimentally forces between a fibronectin-coated surface (i.e., proxy for an implanted cardiac device) and fibronectin-binding receptors on the surface of individual living bacteria from each of 80 clinical isolates of S. aureus. These isolates originated from humans with infected cardiac devices (CDI; n = 26), uninfected cardiac devices (n = 20), and the anterior nares of asymptomatic subjects (n = 34). CDI isolates exhibited a distinct binding-force signature and had specific single amino acid polymorphisms in fibronectin-binding protein A corresponding to E652D, H782Q, and K786N. In silico molecular dynamics simulations demonstrate that residues D652, Q782, and N786 in fibronectin-binding protein A form extra hydrogen bonds with fibronectin, complementing the higher binding force and energy measured by atomic force microscopy for the CDI isolates. This study is significant, because it links pathogenic bacteria biofilms from the length scale of bonds acting across a nanometer-scale space to the clinical presentation of disease at the human dimension.
Asunto(s)
Adhesinas Bacterianas/genética , Marcapaso Artificial/microbiología , Polimorfismo Genético , Staphylococcus aureus/metabolismo , Adhesinas Bacterianas/química , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Biopelículas , Humanos , Microscopía de Fuerza Atómica , Simulación de Dinámica Molecular , Datos de Secuencia Molecular , Homología de Secuencia de AminoácidoRESUMEN
Staphylococcus aureus is part of the indigenous microbiota of humans. Sometimes, S. aureus bacteria enter the bloodstream, where they form infections on implanted cardiovascular devices. A critical, first step in such infections is a bond that forms between fibronectin-binding protein (FnBP) on S. aureus and host proteins, such as fibronectin (Fn), that coat the surface of implants in vivo. In this study, native FnBPs on living S. aureus were shown to form a mechanically strong conformational structure with Fn by atomic force microscopy. The tensile acuity of this bond was probed for 46 bloodstream isolates, each from a patient with a cardiovascular implant. By analyzing the force spectra with the worm-like chain model, we determined that the binding events were consistent with a multivalent, cluster bond consisting of ~10 or ~80 proteins in parallel. The dissociation rate constant (k(off), s(-1)) of each multibond complex was determined by measuring strength as a function of the loading rate, normalized by the number of bonds. The bond lifetime (1/k(off)) was two times longer for bloodstream isolates from patients with an infected device (1.79 or 69.47 s for the 10- or 80-bond clusters, respectively; n = 26 isolates) relative to those from patients with an uninfected device (0.96 or 34.02 s; n = 20 isolates). This distinction could not be explained by different amounts of FnBP, as confirmed by Western blots. Rather, amino acid polymorphisms within the Fn-binding repeats of FnBPA explain, at least partially, the statistically (p < 0.05) longer bond lifetime for isolates associated with an infected cardiovascular device.
Asunto(s)
Adhesinas Bacterianas/metabolismo , Fibronectinas/metabolismo , Infecciones Relacionadas con Prótesis/metabolismo , Infecciones Estafilocócicas/metabolismo , Staphylococcus aureus/metabolismo , Adhesinas Bacterianas/química , Anciano , Anciano de 80 o más Años , Bacteriemia/metabolismo , Bacteriemia/microbiología , Biopelículas/crecimiento & desarrollo , Desfibriladores Implantables/microbiología , Femenino , Humanos , Masculino , Microscopía de Fuerza Atómica , Persona de Mediana Edad , Modelos Químicos , Marcapaso Artificial/microbiología , Infecciones Relacionadas con Prótesis/microbiología , Unión Proteica/fisiología , Infecciones Estafilocócicas/microbiología , Staphylococcus aureus/crecimiento & desarrollo , Resistencia a la TracciónRESUMEN
Inorganic salts in marine aerosols play an active role in atmospheric chemistry, particularly in coastal urban regions. The study of the interactions of these ions with water molecules at the aqueous surface helps to elucidate the role of inorganic cations and anions in atmospheric processes. We present surface vibrational sum frequency generation (SFG) spectroscopic and molecular dynamics (MD) studies of aqueous MgCl(2) surfaces as models of marine aerosol. Spectroscopy results reveal that the disturbance of the hydrogen bonding environment of the air/aqueous interface is dependent on the MgCl(2) concentration. At low concentrations (< 1 M) minor changes are observed. At concentrations above 1 M the hydrogen bonding environment is highly perturbed. The 2.1 M intermediate concentration solution shows the largest SFG response relative to the other solutions including concentrations as high as 4.7 M. The enhancement of SFG signal observed for the 2.1 M solution is attributed to a larger SFG-active interfacial region and more strongly oriented water molecules relative to other concentrations. MD simulations reveal concentration dependent compression of stratified layers of ions and water orientation differences at higher concentrations. SFG and MD studies of the dangling OH of the surface water reveal that the topmost water layer is affected structurally at high concentrations (> 3.1 M). Finally, the MgCl(2) concentration effect on a fatty acid coated aqueous surface was investigated and SFG spectra reveal that deprotonation of the carboxylic acid of atmospherically relevant palmitic acid (PA) is accompanied by binding of the Mg(2+) to the PA headgroup.
Asunto(s)
Aerosoles , Iones , Cloruro de Magnesio/química , Aniones , Atmósfera , Ácidos Carboxílicos/química , Cationes , Simulación por Computador , Enlace de Hidrógeno , Magnesio/química , Modelos Estadísticos , Ácido Palmítico/química , Sales (Química)/química , Espectrofotometría/métodos , Agua/químicaRESUMEN
Fibronectin (Fn) and fibrinogen (Fg) are major host proteins present in the extracellular matrix, blood, and coatings on indwelling medical devices. The ability of Staphylococcus aureus to cause infections in humans depends on favorable interactions with these host ligands. Closely related bacterial adhesins, fibronectin-binding proteins A and B (FnBPA, FnBPB) were evaluated for two key steps in pathogenesis: clumping and adhesion. Experiments utilized optical spectrophotometry, flow cytometry, and atomic force microscopy to probe FnBPA/B alone or in combination in seven different strains of S. aureus and Lactococcus lactis, a Gram-positive surrogate that naturally lacks adhesins to mammalian ligands. In the absence of soluble ligands, both FnBPA and FnBPB were capable of interacting with adjacent FnBPs from neighboring bacteria to mediate clumping. In the presence of soluble host ligands, clumping was enhanced particularly under shear stress and with Fn present in the media. FnBPB exhibited greater ability to clump compared to FnBPA. The strength of adhesion was similar for immobilized Fn to FnBPA and FnBPB. These findings suggest that these two distinct but closely related bacterial adhesins, have different functional capabilities to interact with host ligands in different settings (e.g., soluble vs. immobilized). Survival and persistence of S. aureus in a human host may depend on complementary roles of FnBPA and FnBPB as they interact with different conformations of Fn or Fg (compact in solution vs. extended on a surface) present in different physiological spaces.
Asunto(s)
Infecciones Estafilocócicas , Staphylococcus aureus , Adhesinas Bacterianas , Animales , Proteínas Bacterianas , Fibrinógeno , Fibronectinas , Humanos , LigandosRESUMEN
It is well established that bacteria are able to respond to temporal gradients (e.g., by chemotaxis). However, it is widely held that prokaryotes are too small to sense spatial gradients. This contradicts the common observation that the vast majority of bacteria live on the surface of a solid substrate (e.g., as a biofilm). Herein we report direct experimental evidence that the nonmotile bacterium Staphylococcus aureus possesses a tactile response, or primitive sense of touch, that allows it to respond to spatial gradients. Attached cells recognize their substrate interface and localize adhesins toward that region. Braille-like avidity maps reflect a cell's biochemical sensory response and reveal ultrastructural regions defined by the actual binding activity of specific proteins.
Asunto(s)
Staphylococcus aureus/fisiología , Adhesinas Bacterianas/química , Adhesinas Bacterianas/genética , Adhesinas Bacterianas/fisiología , Adhesión Bacteriana/fisiología , Biopelículas/crecimiento & desarrollo , Fenómenos Biofísicos , Fibronectinas/química , Fibronectinas/genética , Fibronectinas/fisiología , Microscopía de Fuerza Atómica , Modelos Biológicos , Dominios y Motivos de Interacción de Proteínas , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Staphylococcus aureus/genética , Propiedades de SuperficieRESUMEN
Sodium chloride solutions have been used extensively as a model of seawater in both theoretical and experimental studies of the chemistry of sea salt aerosol. Many groups have found that chloride anions are present at the air-solution interface. This observation has been important for the development of a mechanism for the heterogeneous production of molecular chlorine from chloride in sea salt aerosol. However, while sodium chloride is a major constituent of seawater, it is by no means the only salt present. Seawater contains one Mg(2+) for every eight Na(+). Mg(2+) is naturally occurring in ocean waters from mineral deposits in the Earth's crust and biological sources. Mg(2+) forms a hexahydrate structure, rather than contact ion pairs with chloride anion, and this impacts the ordering of water in solution. In this study, we use molecular dynamics simulations, ab initio calculations, and vibrational sum frequency generation (SFG) spectroscopy to explore the effect of the Mg(2+) cation and its tightly bound solvation shell on the surface propensity of chloride, ion-ion interactions, and water structure of the air-solution interface of concentrated chloride salt solutions. In addition, we provide molecular level details that may be relevant to the heterogeneous reactions of chloride in deliquesced sea salt aerosols. In particular, we show that the presence of the divalent Mg(2+) cation does not modify the surface propensity of chloride compared to Na(+) and hence, its availability to interfacial reaction, although some differences in the behavior of chloride may occur due to specific ion interactions. In this work, we also discuss the SFG free OH band at the surface of salt solutions and conclude that it is often not straightforward to interpret.
Asunto(s)
Cloruro de Magnesio/química , Agua de Mar/química , Cloruro de Sodio/química , Aire , Modelos Moleculares , Conformación Molecular , Teoría Cuántica , Soluciones , Análisis Espectral , VibraciónRESUMEN
Magnesium dication plays many significant roles in biochemistry. While it is available to the environment from both ocean waters and mineral salts on land, its roles in environmental and atmospheric chemistry are still relatively unknown. Several pieces of experimental evidence suggest that contact ion pairing may not exist at ambient conditions in solutions of magnesium chloride up to saturation concentrations. This is not typical of most ions. There has been disagreement in the molecular dynamics literature concerning the existence of ion pairing in magnesium chloride solutions. Using a force field developed during this study, we show that contact ion pairing is not energetically favorable. Additionally, we present a concentration-dependent Raman spectroscopic study of the Mg-O(water) hexaaquo stretch that clearly supports the absence of ion pairing in MgCl(2) solutions, although a transition occurring in the spectrum between 0.06x and 0.09x suggests a change in solution structure. Finally, we compare experimental and calculated observables to validate our force field as well as two other commonly used magnesium force fields, and in the process show that ion pairing of magnesium clearly is not observed at higher concentrations in aqueous solutions of magnesium chloride, independent of the choice of magnesium force field, although some force fields give better agreement to experimental results than others.
Asunto(s)
Cloruro de Magnesio/química , Simulación de Dinámica Molecular , Vibración , Agua/química , Reproducibilidad de los Resultados , Soluciones , Espectrometría Raman , Difracción de Rayos XRESUMEN
Vibrational sum frequency generation (VSFG) spectroscopy and molecular dynamics (MD) simulations are used to study the surface residence and organization of gas-phase methyl halide and methyl alcohol molecules adsorbed to the air/water interface, while Raman spectroscopy is used to detect the uptake of the gas-phase species into the bulk aqueous phase. Spectroscopy results reveal the presence of methyl alcohol in the bulk and at the surface. Methyl chloride is detected in the bulk, but not at the surface. This indicates that methyl alcohol adsorbs to the aqueous surface in a layer that is ordered, in agreement with previous studies, and is also readily taken up into the bulk aqueous phase, whereas methyl chloride adsorbs, but, while being taken up into the bulk liquid, has lower surface number density and/or forms a more disordered surface layer than methyl alcohol. MD simulations show that methyl halide molecules transition readily between the gas phase and interface, resulting in significantly shorter residence times at the surface for the methyl halides relative to methyl alcohol. Both the geometries that the methyl species adopt at the interface and the interactions between the methyl species and the interfacial water molecules differ for the halides and the alcohol. Complementary studies of butyl species show similar results: butyl alcohol adsorbs to the aqueous surface in a layer that exhibits a certain degree of order corresponding to the chains aligned along the surface normal, while a markedly more disordered surface layer and shorter residence times are observed in MD simulations for the butyl halides as compared to the alcohol. Desorption from the interface was found to be less frequent for the butyl halides than for the methyl halides by MD simulations. Although Raman studies show uptake of the butyl alcohol into the bulk phase, neither Raman studies nor MD simulations provide any evidence for uptake of the butyl halides into the bulk phase. The profound difference in preferred orientations between alkyl halides and alcohols at the aqueous surface, with the halogen atom of the alkyl halides being to a large degree exposed to the vapor phase, is likely to have consequences for chemistry of alkyl halides adsorbed on the surface of atmospheric aerosol particles.
Asunto(s)
Aire/análisis , Metanol/análisis , Cloruro de Metilo/análisis , Agua/análisis , Adsorción , Metanol/química , Cloruro de Metilo/química , Modelos Químicos , Espectrometría Raman , Propiedades de Superficie , VibraciónRESUMEN
Fibronectin-binding protein A (FnBPA), a protein displayed on the outer surface of Staphylococcus aureus, has a structured A-domain that binds fibrinogen (Fg) and a disordered repeat-region that binds fibronectin (Fn). Amino acid substitutions in Fn-binding repeats (FnBRs) have previously been linked to cardiovascular infection in humans. Here we used microtiter and atomic force microscopy (AFM) to investigate adhesion by variants of full-length FnBPA covalently anchored in the outer cell wall of Lactococcus lactis, a Gram-positive surrogate that otherwise lacks adhesins to mammalian ligands. Fn adhesion increased in five of seven FnBPA variants under static conditions. The bond targeting Fn increased its strength with load under mechanical dissociation. Substitutions extended bond lifetime (1/koff) up to 2.1 times for FnBPA-Fn. Weaker adhesion was observed for Fg in all FnBPA variants tested with microtiter. However, mechanical dissociation with AFM showed significantly increased tensile strength for Fg interacting with the E652D/H782Q variant. This is consistent with a force-induced mechanism and suggests that the dock, lock, and latch (DLL) mechanism is favored for Fg-binding under mechanical stress. Collectively, these experiments reveal that FnBPA exhibits bimodal, ligand-dependent adhesive behavior. Amino acid substitutions in the repeat-region of FnBPA impact binding to both ligands. This was unexpected for Fg since all variants have the same A-domain sequence, and the Fg-binding site is distant from the repeat region. This indicates that FnBRs may fold back on the A-domain in a way that impacts the DLL binding mechanism for Fg.
Asunto(s)
Adhesinas Bacterianas/metabolismo , Sustitución de Aminoácidos , Fibrinógeno/metabolismo , Staphylococcus aureus/metabolismo , Secuencias Repetidas Terminales , Adhesinas Bacterianas/química , Lactococcus lactis/metabolismo , Unión ProteicaRESUMEN
Atomic force microscopy (AFM) was used in concert with transmission electron microscopy (TEM) to image magnetotactic bacteria (Magnetospirillum gryphiswaldense MSR-1 and Magnetospirillum magneticum AMB-1), magnetosomes, and purified Mms6 proteins. Mms6 is a protein that is associated with magnetosomes in M. magneticum AMB-1 and is believed to control the synthesis of magnetite (Fe(3)O(4)) within the magnetosome. We demonstrated how AFM can be used to capture high-resolution images of live bacteria and achieved nanometer resolution when imaging Mms6 protein molecules on magnetite. We used AFM to acquire simultaneous topography and amplitude images of cells that were combined to provide a three-dimensional reconstructed image of M. gryphiswaldense MSR-1. TEM was used in combination with AFM to image M. gryphiswaldense MSR-1 and magnetite-containing magnetosomes that were isolated from the bacteria. AFM provided information, such as size, location and morphology, which was complementary to the TEM images.
Asunto(s)
Óxido Ferrosoférrico , Magnetosomas/ultraestructura , Magnetospirillum/ultraestructura , Microscopía de Fuerza Atómica , Microscopía Electrónica de TransmisiónRESUMEN
Hydration and orientation of the phosphate group of dipalmitoylphosphatidylcholine (DPPC) monolayers in the liquid-expanded (LE) phase and the liquid-condensed (LC) phase in the presence of sodium ions and calcium ions was investigated with vibrational sum frequency generation (SFG) spectroscopy at the air-aqueous interface in conjunction with surface pressure measurements. In the LE phase, both sodium and calcium affect the phosphate group hydration. In the LC phase, however, sodium ions affect the phosphate hydration subtly, while calcium ions cause a marked dehydration. Silica-supported DPPC monolayers prepared by the Langmuir-Blodgett method reveal similar hydration behavior relative to that observed in the corresponding aqueous subphase for the case of water and in the presence of sodium ions. However, in the presence of calcium ions the phosphate group dehydration is greater than that from the corresponding purely aqueous CaCl(2) subphase. The average tilt angles from the surface normal of the PO(2)(-) group of DPPC monolayers on the water surface and on the silica substrate calculated from SFG data are found to be 59 degrees +/- 3 degrees and 72 degrees +/- 5 degrees , respectively. Orientation of the phosphate group is additionally affected by the presence of ions. These findings show that extrapolation of results obtained from model membranes from liquid surfaces to solid supports may not be warranted since there are differences in headgroup organization on the two subphases.
Asunto(s)
1,2-Dipalmitoilfosfatidilcolina/química , Aire , Calcio/química , Dióxido de Silicio/química , Sodio/química , Agua/química , Espectrofotometría InfrarrojaRESUMEN
An account is given of the current state of understanding of aqueous salt, acid, and lipid/water surfaces, interfacial depth, and molecular organization within the air-solution interfacial region. Water structure, hydration, surface propensity of solutes, and surface organization are discussed. In this perspective, vibrational sum frequency generation spectroscopic studies of aqueous surfaces are interpreted. Comment on future directions within the field of aqueous surface structure is provided.