RESUMEN
A new coumestan named 7,5'-dihydroxy-4'-(3''-hydroxy-3''-methyl-trans-isobut-1''-enyl) coumestan (1), together with five known compounds (2-6), was isolated from the EtOAc-soluble extract of the stems of Acanthopanax senticosus. Their structures were elucidated based on extensive spectroscopic analyses. All the isolates were evaluated for in vitro cytotoxic activities against four human cancer cells including HepG2, A549, HeLa and MCF-7. Among them, the new compound 1 was found to exhibit significant cytotoxic activity on HeLa cells with IC50 value of 6.5 µM.
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Antineoplásicos , Eleutherococcus , Eleutherococcus/química , Células HeLa , Humanos , Estructura Molecular , Extractos Vegetales/químicaRESUMEN
By combining the conformal polishing method with short stroke vibration, a novel, to the best of our knowledge, conformal vibration polishing (CVP) method is proposed. The CVP method is expected to be an efficient means of optical processing by its high material removal rate and smoothing characteristics of mid-spatial frequency (MSF) errors. A quantitative time-domain smoothing model and a convergence factor (${\rm CF}_C$) are presented based on the research of smoothing characteristics. The motion mechanism, material removal ability, solution, and expansion of the smoothing model are demonstrated theoretically and experimentally. The experimental results exhibited good agreement with the theoretical predictions for the proposed method. The research provides a certain theoretical foundation for parameter selection and process optimization of the CVP method.
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A new flavonoid named (2S)-7,4'-dimethoxyl-6-(2â³,3â³-epoxy-3â³-methylbutyl)flavanone (1), along with five known compounds (2-6), were isolated from the EtOAc-soluble extract of the stem bark of Maackia amurensis. Their structures were elucidated on the basis of spectroscopic methods. All compounds were evaluated for anti-inflammatory and antioxidant activities in vitro. Among them, compound 5 showed the highest inhibitory activity on NO production in RAW264.7 cells stimulated by LPS with IC50 value of 59.0 ± 1.5 µM. Meanwhile, compounds 1-6 exhibited varying antioxidant activities through DPPH, ABTS free radical-scavenging and FRAP assays.
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Antioxidantes , Maackia , Antiinflamatorios/farmacología , Antioxidantes/farmacología , Estructura Molecular , Extractos VegetalesRESUMEN
Sleep deprivation (SD) has detrimental effects on the physiological function of the brain. However, the underlying mechanism remains elusive. In the present study, we investigated the expression of candidate plasticity-related gene 15 (cpg15), a neurotrophic gene, and its potential role in SD using a REM-SD mouse model. Immunofluorescent and Western blot analysis revealed that the expression of cpg15 protein decreased in the hippocampus, ventral group of the dorsal thalamus (VENT), and somatosensory area of cerebral cortex (SSP) after 24-72 h of REM-SD, and the oxidative stress in these brain regions was increased in parallel, as indicated by the ratio of glutathione (GSH) to its oxidative product (GSSG). Over-expression of cpg15 in thalamus, hippocampus, and cerebral cortex mediated by AAV reduced the oxidative stress in these regions, indicating that the decrease of cpg15 might be a cause that augments oxidative stress in the sleep deprived mouse brain. Collectively, the results imply that cpg15 may play a protective function in the SD-subjected mouse brain via an anti-oxidative function. To our knowledge, this is the first time to provide evidences in the role of cpg15 against SD-induced oxidative stress in the brain.
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Encéfalo/metabolismo , Proteínas del Tejido Nervioso/metabolismo , Estrés Oxidativo , Privación de Sueño/metabolismo , Animales , Encéfalo/patología , Células COS , Chlorocebus aethiops , Proteínas Ligadas a GPI/metabolismo , Glutatión/metabolismo , Masculino , Ratones , Ratones Endogámicos C57BL , Privación de Sueño/patologíaRESUMEN
The present study focuses on the function of cpg15, a neurotrophic factor, in ischemic neuronal recovery using transient global cerebral ischemic (TGI) mouse model and oxygen-glucose deprivation (OGD)-treated primary cultured cells. The results showed that expression of cpg15 proteins in astrocytes, predominantly the soluble form, was significantly increased in mouse hippocampus after TGI and in the cultured astrocytes after OGD. Addition of the medium from the cpg15-overexpressed astrocytic culture into the OGD-treated hippocampal neuronal cultures reduces the neuronal injury, whereas the recovery of neurite outgrowths of OGD-injured neurons was prevented when cpg15 in the OGD-treated astrocytes was knocked down, or the OGD-treated-astrocytic medium was immunoadsorbed by cpg15 antibody. Furthermore, lentivirus-delivered knockdown of cpg15 expression in mouse hippocampal astrocytes diminishes the dendritic branches and exacerbates injury of neurons in CA1 region after TGI. In addition, treatment with inhibitors of MEK1/2, PI3K, and TrkA decreases, whereas overexpression of p-CREB, but not dp-CREB, increases the expression of cpg15 in U118 or primary cultured astrocytes. Also, it is observed that the Flag-tagged soluble cpg15 from the astrocytes transfected with Flag-tagged cpg15-expressing plasmids adheres to the surface of neuronal bodies and the neurites. In conclusion, our results suggest that the soluble cpg15 from astrocytes induced by ischemia could ameliorate the recovery of the ischemic-injured hippocampal neurons via adhering to the surface of neurons. The upregulated expression of cpg15 in astrocytes may be activated via MAPK and PI3K signal pathways, and regulation of CREB phosphorylation.SIGNIFICANCE STATEMENT Neuronal plasticity plays a crucial role in the amelioration of neurological recovery of ischemic injured brain, which remains a challenge for clinic treatment of cerebral ischemia. cpg15 as a synaptic plasticity-related factor may participate in promoting the recovery process; however, the underlying mechanisms are still largely unknown. The objective of this study is to reveal the function and mechanism of neuronal-specific cpg15 expressed in astrocytes after ischemia induction, in promoting the recovery of injured neurons. Our findings provided new mechanistic insight into the neurological recovery, which might help develop novel therapeutic options for cerebral ischemia via astrocytic-targeting interference of gene expression.
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Astrocitos/metabolismo , Isquemia Encefálica/metabolismo , Hipocampo/metabolismo , Proteínas del Tejido Nervioso/biosíntesis , Proyección Neuronal/fisiología , Neuronas/metabolismo , Animales , Astrocitos/patología , Isquemia Encefálica/patología , Línea Celular Tumoral , Células Cultivadas , Proteínas Ligadas a GPI/biosíntesis , Hipocampo/patología , Humanos , Masculino , Ratones , Ratones Endogámicos C57BL , Neuritas/metabolismo , Neuritas/patología , Neuronas/patología , Distribución Aleatoria , Recuperación de la Función/fisiología , SolubilidadRESUMEN
Reactive astrocytes induced by ischemia can transdifferentiate into mature neurons. This neurogenic potential of astrocytes may have therapeutic value for brain injury. Epigenetic modifications are widely known to involve in developmental and adult neurogenesis. PAX6, a neurogenic fate determinant, contributes to the astrocyte-to-neuron conversion. However, it is unclear whether microRNAs (miRs) modulate PAX6-mediated astrocyte-to-neuron conversion. In the present study we used bioinformatic approaches to predict miRs potentially targeting Pax6, and transient middle cerebral artery occlusion (MCAO) to model cerebral ischemic injury in adult rats. These rats were given striatal injection of glial fibrillary acidic protein targeted enhanced green fluorescence protein lentiviral vectors (Lv-GFAP-EGFP) to permit cell fate mapping for tracing astrocytes-derived neurons. We verified that miR-365 directly targets to the 3'-UTR of Pax6 by luciferase assay. We found that miR-365 expression was significantly increased in the ischemic brain. Intraventricular injection of miR-365 antagomir effectively increased astrocytic PAX6 expression and the number of new mature neurons derived from astrocytes in the ischemic striatum, and reduced neurological deficits as well as cerebral infarct volume. Conversely, miR-365 agomir reduced PAX6 expression and neurogenesis, and worsened brain injury. Moreover, exogenous overexpression of PAX6 enhanced the astrocyte-to-neuron conversion and abolished the effects of miR-365. Our results demonstrate that increase of miR-365 in the ischemic brain inhibits astrocyte-to-neuron conversion by targeting Pax6, whereas knockdown of miR-365 enhances PAX6-mediated neurogenesis from astrocytes and attenuates neuronal injury in the brain after ischemic stroke. Our findings provide a foundation for developing novel therapeutic strategies for brain injury.
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Astrocitos/metabolismo , MicroARNs/metabolismo , Neurogénesis/fisiología , Neuronas/metabolismo , Factor de Transcripción PAX6/metabolismo , Accidente Cerebrovascular/metabolismo , Animales , Antagomirs/administración & dosificación , Astrocitos/patología , Encéfalo/metabolismo , Encéfalo/patología , Isquemia Encefálica/metabolismo , Isquemia Encefálica/patología , Hipoxia de la Célula/fisiología , Células Cultivadas , Modelos Animales de Enfermedad , Glucosa/deficiencia , Masculino , MicroARNs/antagonistas & inhibidores , Neuronas/patología , Ratas Sprague-Dawley , Accidente Cerebrovascular/patologíaRESUMEN
To determine whether reactive astrocytes stimulated by brain injury can transdifferentiate into functional new neurons, we labeled these cells by injecting a glial fibrillary acidic protein (GFAP) targeted enhanced green fluorescence protein plasmid (pGfa2-eGFP plasmid) into the striatum of adult rats immediately following a transient middle cerebral artery occlusion (MCAO) and performed immunolabeling with specific neuronal markers to trace the neural fates of eGFP-expressing (GFP(+)) reactive astrocytes. The results showed that a portion of striatal GFP(+) astrocytes could transdifferentiate into immature neurons at 1 week after MCAO and mature neurons at 2 weeks as determined by double staining GFP-expressing cells with ßIII-tubulin (GFP(+)-Tuj-1(+)) and microtubule associated protein-2 (GFP(+)-MAP-2(+)), respectively. GFP(+) neurons further expressed choline acetyltransferase, glutamic acid decarboxylase, dopamine receptor D2-like family proteins, and the N-methyl-D-aspartate receptor subunit R2, indicating that astrocyte-derived neurons could develop into cholinergic or GABAergic neurons and express dopamine and glutamate receptors on their membranes. Electron microscopy analysis indicated that GFP(+) neurons could form synapses with other neurons at 13 weeks after MCAO. Electrophysiological recordings revealed that action potentials and active postsynaptic currents could be recorded in the neuron-like GFP(+) cells but not in the astrocyte-like GFP(+) cells, demonstrating that new GFP(+) neurons possessed the capacity to fire action potentials and receive synaptic inputs. These results demonstrated that striatal astrocyte-derived new neurons participate in the rebuilding of functional neural networks, a fundamental basis for brain repair after injury. These results may lead to new therapeutic strategies for enhancing brain repair after ischemic stroke.
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Astrocitos/fisiología , Isquemia Encefálica/fisiopatología , Cuerpo Estriado/fisiopatología , Neurogénesis/fisiología , Neuronas/fisiología , Accidente Cerebrovascular/fisiopatología , Animales , Astrocitos/patología , Isquemia Encefálica/patología , Colina O-Acetiltransferasa/metabolismo , Cuerpo Estriado/patología , Modelos Animales de Enfermedad , Proteína Ácida Fibrilar de la Glía/genética , Proteína Ácida Fibrilar de la Glía/metabolismo , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Infarto de la Arteria Cerebral Media , Masculino , Proteínas Asociadas a Microtúbulos/metabolismo , Neuronas/patología , Ratas Sprague-Dawley , Receptores de Dopamina D2/metabolismo , Receptores de N-Metil-D-Aspartato/metabolismo , Accidente Cerebrovascular/patología , Sinapsis/patología , Sinapsis/fisiología , Técnicas de Cultivo de Tejidos , Tubulina (Proteína)/metabolismo , Ácido gamma-Aminobutírico/metabolismoRESUMEN
This study focuses on the function of NSSR1, a splicing factor, in neuronal injury in the ischemic mouse brain using the transient global cerebral ischemic mouse model and the cultured cells treated with oxygen-glucose deprivation (OGD). The results showed that the cerebral ischemia triggers the expression of NSSR1 in hippocampal astrocytes, predominantly the dephosphorylated NSSR1 proteins, and the Exon3 inclusive NCAM-L1 variant and the Exon4 inclusive CREB variant. While in the hippocampus of astrocyte-specific NSSR1 conditional knockdown (cKD) mice, where cerebral ischemia no longer triggers NSSR1 expression in astrocytes, the expression of Exon3 inclusive NCAM-L1 variant and Exon4 inclusive CREB variant were no longer triggered as well. In addition, the injury of hippocampal neurons was more severe in astrocyte-specific NSSR1 cKD mice compared with in wild-type mice after brain ischemia. Of note, the culture media harvested from the astrocytes with overexpression of NSSR1 or the Exon3 inclusive NCAM-L1 variant, or Exon4 inclusive CREB variant were all able to reduce the neuronal injury induced by OGD. The results provide the evidence demonstrating that: (1) Splicing factor NSSR1 is a new factor involved in reducing ischemic injury. (2) Ischemia induces NSSR1 expression in astrocytes, not in neurons. (3) NSSR1-mediated pathway in astrocytes is required for reducing ischemic neuronal injury. (4) NCAM-L1 and CREB are probably mediators in NSSR1-mediated pathway. In conclusion, our results suggest for the first time that NSSR1 may provide a novel mechanism for reducing neuronal injury after ischemia, probably through regulation on alternative splicing of NCAM-L1 and CREB in astrocytes.
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Proteínas de Ciclo Celular/metabolismo , Regulación de la Expresión Génica/fisiología , Hipocampo/patología , Ataque Isquémico Transitorio/patología , Proteínas de Neoplasias/metabolismo , Neuronas/metabolismo , Proteínas de Unión al ARN/metabolismo , Proteínas Represoras/metabolismo , Animales , Antígeno CD56/genética , Antígeno CD56/metabolismo , Proteína de Unión a CREB/metabolismo , Proteínas de Ciclo Celular/genética , Modelos Animales de Enfermedad , Regulación de la Expresión Génica/genética , Proteína Ácida Fibrilar de la Glía/metabolismo , Glucosa/deficiencia , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Hipoxia/patología , Inmunoprecipitación , Masculino , Ratones , Ratones Noqueados , Proteínas de Neoplasias/genética , Neuroblastoma/patología , Fosforilación/genética , Proteínas de Unión al ARN/genética , Proteínas Represoras/genéticaRESUMEN
The receptor for advanced glycation end products (RAGE) gene expresses two major alternative splicing isoforms, full-length membrane-bound RAGE (mRAGE) and secretory RAGE (esRAGE). Both isoforms play important roles in Alzheimer's disease (AD) pathogenesis, either via interaction of mRAGE with ß-amyloid peptide (Aß) or inhibition of the mRAGE-activated signaling pathway. In the present study, we showed that heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) and Transformer2ß-1 (Tra2ß-1) were involved in the alternative splicing of mRAGE and esRAGE. Functionally, two factors had an antagonistic effect on the regulation. Glucose deprivation induced an increased ratio of mRAGE/esRAGE via up-regulation of hnRNP A1 and down-regulation of Tra2ß-1. Moreover, the ratios of mRAGE/esRAGE and hnRNP A1/Tra2ß-1 were increased in peripheral blood mononuclear cells from AD patients. The results provide a molecular basis for altered splicing of mRAGE and esRAGE in AD pathogenesis. The receptor for advanced glycation end products (RAGE) gene expresses two major alternative splicing isoforms, membrane-bound RAGE (mRAGE) and secretory RAGE (esRAGE). Both isoforms play important roles in Alzheimer's disease (AD) pathogenesis. Mechanism for imbalanced expression of these two isoforms in AD brain remains elusive. We proposed here a hypothetic model to illustrate that impaired glucose metabolism in AD brain may increase the expression of splicing protein hnRNP A1 and reduce Tra2ß-1, which cause the imbalanced expression of mRAGE and esRAGE.
Asunto(s)
Enfermedad de Alzheimer/patología , Encéfalo/metabolismo , Ribonucleoproteína Heterogénea-Nuclear Grupo A-B/metabolismo , Proteínas del Tejido Nervioso/metabolismo , Proteínas de Unión al ARN/metabolismo , Receptores Inmunológicos/genética , Empalmosomas/metabolismo , Anciano , Línea Celular Tumoral , Células Cultivadas , Femenino , Regulación de la Expresión Génica/genética , Glucosa/deficiencia , Ribonucleoproteína Nuclear Heterogénea A1 , Ribonucleoproteína Heterogénea-Nuclear Grupo A-B/genética , Ribonucleoproteínas Nucleares Heterogéneas , Humanos , Leucocitos Mononucleares , Masculino , Modelos Biológicos , Proteínas del Tejido Nervioso/genética , Neuroblastoma/patología , ARN Mensajero/metabolismo , Proteínas de Unión al ARN/genética , Receptor para Productos Finales de Glicación Avanzada , Receptores Inmunológicos/metabolismo , Factores de Empalme Serina-Arginina , Empalmosomas/genética , TransfecciónRESUMEN
The serine/arginine-rich (SR) proteins are one type of major actors in regulation of pre-mRNA splicing. Their functions are closely related to the intracellular spatial organization. The RS domain and phosphorylation status of SR proteins are two critical factors in determining the subcellular distribution. Mammalian Transformer-2ß (Tra2ß) protein, a member of SR proteins, is known to play multiple important roles in development and diseases. In the present study, we characterized the subcellular and subnuclear localization of Tra2ß protein and its related mechanisms. The results demonstrated that in the brain the nuclear and cytoplasmic localization of Tra2ß were correlated with its phosphorylation status. Using deletional mutation analysis, we showed that the nuclear localization of Tra2ß was determined by multiple nuclear localization signals (NLSs) in the RS domains. The point-mutation analysis disclosed that phosphorylation of serine residues in the NLSs inhibited the function of NLS in directing Tra2ß to the nucleus. In addition, we identified at least two nuclear speckle localization signals within the RS1 domain, but not in the RS2 domain. The nuclear speckle localization signals determined the localization of RS1 domain-contained proteins to the nuclear speckle. The function of the signals did not depend on the presence of serine residues. The results provide new insight into the mechanisms by which the subcellular and subnuclear localization of Tra2ß proteins are regulated.
Asunto(s)
Proteínas del Tejido Nervioso/metabolismo , Señales de Localización Nuclear/metabolismo , Proteínas de Unión al ARN/metabolismo , Serina/química , Animales , Encéfalo/metabolismo , Línea Celular Tumoral , Núcleo Celular/metabolismo , Citoplasma/metabolismo , Regulación de la Expresión Génica , Humanos , Microscopía Confocal/métodos , Microscopía Fluorescente/métodos , Fosforilación , Plásmidos/metabolismo , Mutación Puntual , Estructura Terciaria de Proteína , Empalme del ARN , ARN Mensajero/metabolismo , Factores de Empalme Serina-Arginina , Fracciones Subcelulares/metabolismoRESUMEN
Oxidative stress is becoming increasingly implicated in the development of a variety of neurological disorders. However, the underlying mechanism remains elusive. In the present study, we investigated the function and related signal pathway which Cpg15, a neuronal-specific expressed neurotrophic factor, plays in the oxidative stress of neurons using a H2O2-treated N2a cell model. The results showed that the Cpg15 expression was decreased under oxidative stress, and overexpression of Cpg15 increased the activity of antioxidative SOD enzymes and decreased the expression level of prooxidative COX2 enzyme, and the level of oxidative products malondialdehyde (MDA), indicating its function and potential mechanism in alleviating the oxidative stress of cells. The results also indicated that the Nrf2/HO-1 antioxidative pathway was involved in the Cpg15-mediated alleviation of oxidative stress. Also, overexpression of Cpg15 activated the Nrf2 antioxidative pathway in the thalamus of the REM sleep-deprived mice. In conclusion, our results implied that supplemental expression of Cpg15 may alleviate oxidative stress in neuronal cells via regulating the redox enzymes or activating the Nrf2 antioxidant pathway.
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Antioxidantes , Factor 2 Relacionado con NF-E2 , Animales , Antioxidantes/metabolismo , Proteínas Ligadas a GPI , Peróxido de Hidrógeno , Ratones , Factor 2 Relacionado con NF-E2/metabolismo , Proteínas del Tejido Nervioso , Neuronas/metabolismo , Oxidación-Reducción , Estrés OxidativoRESUMEN
Extracellular vesicles (EVs) have found diverse applications in clinical theranostics. However, the current techniques to isolate plasma EVs suffer from burdensome procedures and limited yield. Herein, we report a rapid and efficient EV isolation platform, namely, EV-FISHER, constructed from the metal-organic framework featuring cleavable lipid probes (PO4 3- -spacer-DNA-cholesterol, PSDC). The EV-FISHER baits EVs from plasma by cholesterol and separates them with an ordinary centrifuge. The captured EVs could be released and collected upon subsequent cleavage of PSDC by deoxyribonuclease I. We conclude that EV-FISHER dramatically outperforms the ultracentrifugation (UC) in terms of time (â¼40 min vs. 240 min), isolation efficiency (74.2% vs. 18.1%), and isolation requirement (12,800 g vs. 135,000 g). In addition to the stable performance in plasma, EV-FISHER also exhibited excellent compatibility with downstream single-EV flow cytometry, enabling the identification of glypican-1 (GPC-1) EVs for early diagnosis, clinical stages differentiation, and therapeutic efficacy evaluation in breast cancer cohorts. This work portrays an efficient strategy to isolate EVs from complicated biological fluids with promising potential to facilitate EVs-based theranostics.
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Vesículas Extracelulares , Ultracentrifugación/métodos , Plasma , Citometría de FlujoRESUMEN
Neural salient serine/arginine-rich protein 1 (NSSR1) has been found to play important roles in inhibiting alternative splicing during heat shock and mitosis and is predominantly expressed in neural tissues such as cerebral neurons, cerebellar Purkinje cells and bipolar cells of the retina. Recently, NSSR1 has also been shown to be highly expressed in the testes, suggesting its potential roles in reproductive system. In this report, the expression of NSSR1 in the columnar epithelium of the endometrium and gland epithelium during the development of the mouse uterus, the regulation of NSSR1 level by testosterone in the adult mouse uterus, and expression level of NSSR1 in both human endometrial carcinomas and ovarian cancers were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, and immunohistochemistry. We demonstrated that the expression of NSSR1 was developmentally regulated in the columnar epithelium of the endometrium and gland epithelium in the mouse uterus. Additionally, the NSSR1 level in the mouse uterus was maintained and regulated by testosterone. Interestingly, an enhanced level of NSSR1 was observed in both human endometrial carcinomas and ovarian cancers. Our results suggest that expression and distribution of NSSR1 is developmentally and hormonally regulated and up-regulated in endometrial carcinomas as well as ovarian cancers, indicating its potential involvement in uterine development and tumorgenesis.
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Biomarcadores de Tumor/metabolismo , Proteínas de Ciclo Celular/metabolismo , Neoplasias Endometriales/metabolismo , Proteínas de Neoplasias/metabolismo , Proteínas de Unión al ARN/metabolismo , Proteínas Represoras/metabolismo , Testosterona/farmacología , Útero/metabolismo , Animales , Estudios de Casos y Controles , Proteínas de Ciclo Celular/efectos de los fármacos , Neoplasias Endometriales/patología , Femenino , Regulación Neoplásica de la Expresión Génica , Humanos , Ratones , Ratones Endogámicos , Modelos Animales , Proteínas de Neoplasias/efectos de los fármacos , Neoplasias Ováricas/metabolismo , Neoplasias Ováricas/patología , Proteínas de Unión al ARN/efectos de los fármacos , Proteínas Represoras/efectos de los fármacos , Factores de Empalme Serina-Arginina , Regulación hacia ArribaRESUMEN
The candidate plasticity related gene 15 (cpg15) plays important roles in neural development and plasticity. In the present study, we studied the role of the cpg15 3'-untranslated region (UTR) in regulating the expression of the gene. The results showed that the presence of the 3'-UTR significantly decreases, while loss of a putative AU-rich element (ARE) in the 3'-UTR increases the cpg15 expression, indicating that the 3'-UTR and ARE may be essential for regulation of cpg15 expression. In addition, HuD, a neural-specific RNA binding protein, increased the cpg15 expression, which depends on the presence of the 3'-UTR and ARE. RNA-binding protein immunoprecipitation (RIP) assay demonstrated that HuD forms a complex with cpg15 mRNA in the cells of rat hippocampus. Deletion of HuD domains RRM1 plus RRM2 or Hinge region plus RRM3 attenuates the function of HuD in enhancing the cpg15 expression. The results suggest that HuD regulates the cpg15 expression via the 3'-UTR-mediated mechanism, which requires the presence of the ARE.
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Regiones no Traducidas 3' , Proteínas de la Membrana/genética , Proteínas del Tejido Nervioso/genética , Animales , Secuencia de Bases , Western Blotting , Línea Celular Tumoral , Cartilla de ADN , Humanos , Inmunoprecipitación , Ratas , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
Aiming to improve the comprehensive road performance of asphalt binders, especially the high-temperature performance, a novel asphalt binder was prepared by compounding high-quality and low-cost polyethylene (PE) with graphene (GNPs) using a high-speed shearing machine. The rheological properties and interaction mechanism of PE/GNPs composite modified asphalt were investigated using temperature sweep (TeS), multiple stress creep recovery (MSCR), linear amplitude sweep (LAS) and Fourier transform infrared spectroscopy (FT-IR) and field emission scanning electron microscopy (FESEM). The experimental results demonstrated that GNPs and PE can synergistically improve the high-temperature performance of asphalt binders and enhance the rutting resistance of pavements; the pre-blended PE/GNPs masterbatch has good medium-temperature fatigue and low-temperature cracking resistance. Meanwhile, PE/GNPs dispersed uniformly in the asphalt matrix, and the microstructure and dispersion of premixed PE/GNPs masterbatch facilitated the asphalt modification. No new absorption peaks appeared in the FT-IR spectra of the composite modified asphalt, indicating that asphalt binders were physically modified with GNPs and PE. These findings may cast light on the feasibility of polyethylene/graphene composite for asphalt modification.
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The heterogeneous nuclear ribonucleoproteins (hnRNPs) play important roles in DNA repairing, cell signaling, telomere biogenesis, and in regulating gene expression at both transcriptional and translational levels. In the present study, we demonstrated that the expression of hnRNP-R1 and hnRNP-R2 is developmentally regulated in rat retina. The neural specific isoform hnRNP-R2 is expressed in 7-day postnatal rat retina, but not in the adult retina. The positive immunohistochemistry signal of hnRNP-R1 is extensively distributed in the outer plexiform layer, inner nuclear layer and ganglion cell layer of rat retina. Double staining experiments showed that the positive signal of hnRNP-R1 is distributed in ON-type bipolar cells and localized in the cytoplasm, dendrites and axon terminals. In addition, the hnRNP-R1 distribution is regulated in rat retina during circadian. The present investigation suggests that hnRNP-R may play roles in retinal development and light-elicited cellular activities.
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Ribonucleoproteínas Nucleares Heterogéneas/biosíntesis , Retina/metabolismo , Animales , Animales Recién Nacidos , Ritmo Circadiano , Regulación de la Expresión Génica , Masculino , Isoformas de Proteínas/biosíntesis , Ratas , Ratas Sprague-Dawley , Retina/crecimiento & desarrolloRESUMEN
Interleukin-17 receptor B (IL-17RB), a member of the IL-17 receptor family activated by IL-17B/IL-17E, has been shown to be involved in inflammatory diseases. However, the regulation and function of IL-17RB in Helicobacter pylori (H. pylori) infection, especially in the early-phase is still unknown. Here, we found that gastric IL-17RB mRNA and protein were decreased in gastric mucosa of both patients and mice infected with H. pylori. In vitro experiments show that IL-17RB expression was down regulated via PI3K/AKT pathway on gastric epithelial cells (GECs) stimulated with H. pylori in a cagA-involved manner, while in vivo studies showed that the effect was partially dependent on cagA expression. IL-17E was also decreased during the early-phase of H. pylori infection, and provision of exogenous IL-17E resulted in increased CD11b+CD11c- myeloid cells accumulation and decreased bacteria colonization within the gastric mucosa. In the early-phase of H. pylori infection, IL-17E-IL-17RB promoted gastric epithelial cell-derived CXCL1/2/5/6 to attract CD11b+CD11c- myeloid cells, and also contributed to host defense by promoting the production of antibacterial protein Reg3a. This study defines a negative regulatory network involving IL-17E, GECs, IL-17RB, CD11b+CD11c- myeloid cells, and Reg3a in the early-phase of H. pylori infection, which results in an impaired host defense within the gastric microenvironment, suggesting IL-17RB as a potential early intervening target in H. pylori infection.
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Antígeno CD11b/inmunología , Antígeno CD11c/inmunología , Mucosa Gástrica/inmunología , Infecciones por Helicobacter/inmunología , Helicobacter pylori/aislamiento & purificación , Células Mieloides/inmunología , Receptores de Interleucina-17/inmunología , Animales , Antígenos CD11/biosíntesis , Antígenos CD11/inmunología , Antígeno CD11b/biosíntesis , Antígeno CD11b/sangre , Antígeno CD11c/biosíntesis , Infecciones por Helicobacter/sangre , Infecciones por Helicobacter/genética , Helicobacter pylori/genética , Humanos , Ratones , Ratones Endogámicos C57BL , ARN Mensajero/genética , ARN Mensajero/inmunología , Receptores de Interleucina-17/biosíntesis , Receptores de Interleucina-17/genéticaRESUMEN
This study focused on the function of hnRNP-R in the regulation of c-fos expression. We demonstrated that hnRNP-R accelerated the rise and decline phases of c-fos mRNAs and Fos proteins, allowing PMA to induce an augmented pulse response of c-fos expression. Then, we examined the role of the c-fos-derived AU-rich element (ARE) in hnRNP-R-regulated mRNA degradation. Studies with the ARE-GFP reporter gene showed that hnRNP-R significantly reduced the expression of GFP with an inserted ARE. Moreover, immunoprecipitation-RT-PCR analysis demonstrated that in R28 cells and rat retinal tissues, the c-fos mRNA was co-immunoprecipitated with hnRNP-R. These findings indicate that hnRNP-R regulates the c-fos expression in retinal cells, and that the ARE of c-fos mRNAs contributes to this regulation.
Asunto(s)
Ribonucleoproteínas Nucleares Heterogéneas/metabolismo , Proteínas Proto-Oncogénicas c-fos/metabolismo , Retina/citología , Retina/metabolismo , Acetato de Tetradecanoilforbol/farmacología , Animales , Línea Celular , Regulación de la Expresión Génica/efectos de los fármacos , Genes Reporteros , Proteínas Fluorescentes Verdes/metabolismo , Inmunoprecipitación , Proteínas Proto-Oncogénicas c-fos/genética , ARN Mensajero/genética , ARN Mensajero/metabolismo , Ratas , Secuencias Reguladoras de Ácido RibonucleicoRESUMEN
Neural salient serine/arginine-rich protein 1 (NSSR1) expression has been found in mouse cerebral neurons, cerebellar Purkinje cells, pyramidal neurons and granule cells of dentate gyrus and regulates the pre-mRNA splicing of genes important for neural functions. In this study, we demonstrated that NSSR1 is expressed in rat retina and extensively distributed in the outer and inner plexiform layers. Double staining experiments showed that NSSR1 distributed mainly in ON-type bipolar cells and localized in the dendrites, somata and axon terminals. The result suggests that NSSR1 may play important roles in retinal function, possibly via regulating the neural-specific alternative splicing of genes.
Asunto(s)
Proteínas del Ojo/metabolismo , Neuronas/metabolismo , Proteínas de Unión al ARN/metabolismo , Retina/metabolismo , Animales , Especificidad de Anticuerpos , Proteínas de Ciclo Celular/genética , Proteínas de Ciclo Celular/metabolismo , Dendritas/metabolismo , Dendritas/ultraestructura , Proteínas del Ojo/genética , Técnica del Anticuerpo Fluorescente , Inmunohistoquímica , Masculino , Proteínas de Neoplasias/genética , Proteínas de Neoplasias/metabolismo , Neuronas/citología , Terminales Presinápticos/metabolismo , Terminales Presinápticos/ultraestructura , Empalme del ARN/fisiología , Proteínas de Unión al ARN/genética , Ratas , Ratas Sprague-Dawley , Proteínas Represoras/genética , Proteínas Represoras/metabolismo , Retina/citología , Células Bipolares de la Retina/citología , Células Bipolares de la Retina/metabolismo , Células Ganglionares de la RetinaRESUMEN
During the early developmental period, long-term potentiation (LTP) can be induced in both vertical and horizontal connections in the rat visual cortex. However, the temporal difference in LTP change between the two pathways during animal development remains unclear. In this study, LTP in vertical (from layer IV to layer II/III) and horizontal (from layer II/III to layer II/III) synaptic connections were recorded in brain slices from the same rats, and the developmental changes of LTP in both directions were compared within the animals' eye-opening period. The results showed that the LTP amplitudes declined to unobservable levels on P16 in the horizontal connections and on P20 in the vertical synaptic connections. Meanwhile, V-LTP (LTP induced in the vertical direction) was always stronger than H-LTP (LTP induced in the horizontal direction) under the same conditions of pairing stimulus (PS). Next, H-LTP and V-LTP were induced from the same neuron in layer II/III to determine the spatiotemporal interactions between layer II/III horizontal inputs and ascending synaptic inputs during the maturation of rat visual cortex. The data show that the weak PS, which failed to induce H-LTP alone, was able to induce H-LTP effectively while V-LTP was performed on P10. Our results suggest that V-LTP can strengthen H-LTP induction in the visual cortex during the early developmental period. In contrast, the regulatory effect of H-LTP on V-LTP was much weaker.