RESUMEN
Cellular delivery is an important concern for the efficiency of medicines and sensors for disease diagnoses and therapy. However, this task is quite challenging. Self-assembly virus capsid proteins might be developed as building blocks for multifunctional cellular delivery vehicles. In this work, we found that SV40 VP1 (Simian virus 40 major capsid protein) could function as a new cell-penetrating protein. The VP1 protein could carry foreign proteins into cells in a pentameric structure. A double color structure, with red QDs (Quantum dots) encapsulated by viral capsids fused with EGFP, was created for imaging cargo delivery and release from viral capsids. The viral capsids encapsulating QDs were further used for cellular delivery of micron-sized iron oxide particles (MPIOs). MPIOs were efficiently delivered into live cells and controlled by a magnetic field. Therefore, our study built virus-based cellular delivery systems for different sizes of cargos: protein molecules, nanoparticles, and micron-sized particles. FROM THE CLINICAL EDITOR: Much research is being done to investigate methods for efficient and specific cellular delivery of drugs, proteins or genetic material. In this article, the authors describe their approach in using self-assembly virus capsid proteins SV40 VP1 (Simian virus 40 major capsid protein). The cell-penetrating behavior provided excellent cellular delivery and should give a new method for biomedical applications.
Asunto(s)
Proteínas de la Cápside/metabolismo , Preparaciones de Acción Retardada/metabolismo , Compuestos Férricos/administración & dosificación , Colorantes Fluorescentes/administración & dosificación , Proteínas Fluorescentes Verdes/administración & dosificación , Puntos Cuánticos/administración & dosificación , Virus 40 de los Simios/metabolismo , Animales , Chlorocebus aethiops , Sistemas de Liberación de Medicamentos/métodos , Compuestos Férricos/análisis , Colorantes Fluorescentes/análisis , Proteínas Fluorescentes Verdes/análisis , Campos Magnéticos , Microscopía Fluorescente , Imagen Óptica , Tamaño de la Partícula , Puntos Cuánticos/análisis , Células VeroRESUMEN
The mechanistic basis for the resistance of Mycobacterium tuberculosis to para-aminosalicylic acid (PAS), an important agent in the treatment of multidrug-resistant tuberculosis, has yet to be fully defined. As a substrate analog of the folate precursor para-aminobenzoic acid, PAS is ultimately bioactivated to hydroxy dihydrofolate, which inhibits dihydrofolate reductase and disrupts the operation of folate-dependent metabolic pathways. As a result, the mutation of dihydrofolate synthase, an enzyme needed for the bioactivation of PAS, causes PAS resistance in M. tuberculosis strain H37Rv. Here, we demonstrate that various missense mutations within the coding sequence of the dihydropteroate (H2Pte) binding pocket of dihydrofolate synthase (FolC) confer PAS resistance in laboratory isolates of M. tuberculosis and Mycobacterium bovis. From a panel of 85 multidrug-resistant M. tuberculosis clinical isolates, 5 were found to harbor mutations in the folC gene within the H2Pte binding pocket, resulting in PAS resistance. While these alterations in the H2Pte binding pocket resulted in reduced dihydrofolate synthase activity, they also abolished the bioactivation of hydroxy dihydropteroate to hydroxy dihydrofolate. Consistent with this model for abolished bioactivation, the introduction of a wild-type copy of folC fully restored PAS susceptibility in folC mutant strains. Confirmation of this novel PAS resistance mechanism will be beneficial for the development of molecular method-based diagnostics for M. tuberculosis clinical isolates and for further defining the mode of action of this important tuberculosis drug.
Asunto(s)
Ácido Aminosalicílico/farmacología , Antibacterianos/farmacología , Mycobacterium tuberculosis/efectos de los fármacos , Péptido Sintasas/fisiología , Alelos , Sitios de Unión/genética , Sitios de Unión/fisiología , Farmacorresistencia Bacteriana/genética , Pruebas de Sensibilidad Microbiana , Mutación Missense/genética , Mutación Missense/fisiología , Mycobacterium bovis/efectos de los fármacos , Mycobacterium bovis/enzimología , Mycobacterium tuberculosis/enzimología , Mycobacterium tuberculosis/genética , Péptido Sintasas/genética , Péptido Sintasas/metabolismoRESUMEN
For living deep-tissue imaging, the optical window favorable for light penetration is in near-infrared wavelengths, which requires fluorescent proteins with emission spectra in the near-infrared region. Here, we report that a single mutant Ser28His of mNeptune with a near-infrared (≥650 nm) emission maxima of 652 nm is found to improve the brightness, photostability, and pH stability when compared with its parental protein mNeptune, while it remains as a monomer, demonstrating that there is still plenty of room to improve the performance of the existing near infrared fluorescence proteins by directed evolution.
Asunto(s)
Concentración de Iones de Hidrógeno , Proteínas Luminiscentes/química , Mutación , Teoría Cuántica , Espectroscopía Infrarroja Corta/métodos , Secuencia de Bases , Cromatografía en Gel , Cartilla de ADN , Datos de Secuencia MolecularRESUMEN
NADH pyrophosphatase (NudC) catalyses the hydrolysis of NAD(H) to AMP and NMN(H) [nicotinamide mononucleotide (reduced form)]. NudC multiple sequence alignment reveals that homologues from most Mycobacterium tuberculosis isolates, but not other mycobacterial species, have a polymorphism at the highly conserved residue 237. To elucidate the functional significance of this polymorphism, comparative analyses were performed using representative NudC isoforms from M. tuberculosis H37Rv (NudC(Rv)) and M. bovis BCG (NudC(BCG)). Biochemical analysis showed that the P237Q polymorphism prevents dimer formation, and results in a loss of enzymatic activity. Importantly, NudC(BCG) was found to degrade the active forms of isoniazid (INH), INH-NAD and ethionamide (ETH), ETH-NAD. Consequently, overexpression of NudC(BCG) in Mycobacterium smegmatis mc(2)155 and M. bovis BCG resulted in a high level of resistance to both INH and ETH. Further genetic studies showed that deletion of the nudC gene in M. smegmatis mc(2)155 and M. bovis BCG resulted in increased susceptibility to INH and ETH. Moreover, inactivation of NudC in both strains caused a defect in drug tolerance phenotype for both drugs in exposure assays. Taken together, these data suggest that mycobacterial NudC plays an important role in the inactivation of INH and ETH.
Asunto(s)
Antituberculosos/metabolismo , Proteínas Bacterianas/metabolismo , Etionamida/metabolismo , Isoniazida/metabolismo , Mycobacterium bovis/enzimología , Mycobacterium smegmatis/enzimología , Mycobacterium tuberculosis/enzimología , Pirofosfatasas/metabolismo , Secuencia de Aminoácidos , Antituberculosos/farmacología , Proteínas Bacterianas/química , Proteínas Bacterianas/genética , Etionamida/farmacología , Isoenzimas/química , Isoenzimas/genética , Isoenzimas/metabolismo , Isoniazida/farmacología , Datos de Secuencia Molecular , Mycobacterium bovis/efectos de los fármacos , Mycobacterium bovis/genética , Mycobacterium bovis/metabolismo , Mycobacterium smegmatis/efectos de los fármacos , Mycobacterium smegmatis/genética , Mycobacterium smegmatis/metabolismo , Mycobacterium tuberculosis/efectos de los fármacos , Mycobacterium tuberculosis/genética , Mycobacterium tuberculosis/metabolismo , Pirofosfatasas/química , Pirofosfatasas/genética , Alineación de SecuenciaRESUMEN
During influenza A virus infection, the NS1 protein is engaged in different functions in different intracellular compartments. In this study, we showed that the NS1 of A/PR/8/34 localized in different positions from that of A/Sydney/5/97 when transiently expressed in Madin-Darby canine kidney cells. Residue 221 of NS1 was identified to be a new key residue involved in the C-terminal nuclear localization signal (NLS) and nucleolar localization signal (NoLS) of NS1 from A/Sydney/5/97. Analysis of chimeric NS1 and further mutants showed that residues responsible for the binding between NS1 and the cleavage and polyadenylation specificity factor (CPSF) are correlated with the intracellular localization of transiently expressed NS1 proteins. Fluorescence loss in photobleaching imaging revealed that the NS1 protein with both functional NLSs and nuclear export signal (NES) was able to shuttle between the nucleus and cytoplasm. Drug inhibition experiments and fluorescence resonance energy transfer analysis suggested that NS1 was exported out of the cell nuclei via a Crm1-independent pathway. Moreover, it is likely that another cytoplasmic localization-related sequence exists in the NS1 protein other than the leucine-rich NES. These findings provide new insights into the mechanism of intracellular localization and trafficking of influenza A virus NS1 protein, which is important for understanding its function.
Asunto(s)
Nucléolo Celular/química , Núcleo Celular/química , Virus de la Influenza A/fisiología , Proteínas no Estructurales Virales/metabolismo , Animales , Línea Celular , Citoplasma/química , Perros , Carioferinas/metabolismo , Proteínas Mutantes/genética , Proteínas Mutantes/metabolismo , Señales de Clasificación de Proteína , Transporte de Proteínas , Receptores Citoplasmáticos y Nucleares/metabolismo , Recombinación Genética , Proteínas no Estructurales Virales/genética , Proteína Exportina 1RESUMEN
Viral capsid-nanoparticle hybrid structures offer new opportunities for nanobiotechnology. We previously generated virus-based nanoparticles (VNPs) of simian virus 40 (SV40) containing quantum dots (QDs) for cellular imaging. However, as an interesting issue of nano-bio interfaces, the mechanism of nanoparticle (NP) encapsulation by viral coat proteins remains unclear. Here, four kinds of QDs with the same core/shell but different surface coatings are tested for encapsulation. All the QDs can be encapsulated efficiently and there is no correlation between the encapsulation efficiency and the surface charge of the QDs. All the SV40 VNPs encapsulating differently modified QDs show similar structures, fluorescence properties, and activity in entering living cells. These results demonstrate the flexibility of SV40 major capsid protein VP1 in NP encapsulation and provide new clues to the mechanism of NP packaging by viral shells.
Asunto(s)
Proteínas de la Cápside/química , Nanopartículas/química , Nanotecnología/métodos , Puntos Cuánticos , Virus 40 de los Simios/metabolismoRESUMEN
The mechanisms of influenza A virus mRNA intracellular transport are still not clearly understood. Here, we visualized the distribution and transport of influenza A virus mRNA in living cells using molecular beacon (MB) technology. Confocal-FRAP measurements determined that the transport of influenza A virus intronless mRNA, in both nucleus and cytoplasm, was energy dependent, being similar to that of Poly(A)(+) RNA. Drug inhibition studies in living cells revealed that the export of influenza A virus mRNA is independent of the CRM1 pathway, while the function of RNA polymerase II (RNAP-II) may be needed. In addition, viral NS1 protein and cellular TAP protein were found associated with influenza A virus mRNA in the cell nucleus. These findings characterize influenza A virus mRNA transport in living cells and suggest that influenza A virus mRNA may be exported from the nucleus by the cellular TAP/p15 pathway with NS1 protein and RNAP-II participation.
Asunto(s)
Virus de la Influenza A/metabolismo , ARN Mensajero/metabolismo , ARN Viral/metabolismo , Animales , Línea Celular , Núcleo Celular/química , Núcleo Celular/virología , Citoplasma/virología , Dactinomicina/farmacología , Perros , Recuperación de Fluorescencia tras Fotoblanqueo , Inmunoprecipitación , Virus de la Influenza A/genética , Carioferinas/metabolismo , Microscopía Confocal , Proteínas de Transporte Nucleocitoplasmático/análisis , Transporte de ARN/efectos de los fármacos , ARN Mensajero/análisis , ARN Viral/análisis , Receptores Citoplasmáticos y Nucleares/metabolismo , Proteínas no Estructurales Virales/análisis , Proteína Exportina 1RESUMEN
Aiming to build a supersensitive and easily operable immunoassay, bifunctional protein nanowires were generated by seeding-induced self-assembling of the yeast amyloid protein Sup35p that genetically fused with protein G and an enzyme (methyl-parathion hydrolase, MPH), respectively. The protein nanowires possessed a high ratio of enzyme molecules to protein G, allowing a dramatic increase of the enzymatic signal when protein G was bound to an antibody target. As a result, a 100-fold enhancement of the sensitivity was obtained when applied in the detection of the Yersinia pestis F1 antigen.
Asunto(s)
Ensayo de Inmunoadsorción Enzimática , Nanocables/química , Proteínas Bacterianas/análisis , Proteínas del Tejido Nervioso/química , Proteínas del Tejido Nervioso/genética , Factores de Terminación de Péptidos/química , Factores de Terminación de Péptidos/genética , Monoéster Fosfórico Hidrolasas/química , Monoéster Fosfórico Hidrolasas/genética , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas de Saccharomyces cerevisiae/química , Proteínas de Saccharomyces cerevisiae/genética , Sensibilidad y EspecificidadRESUMEN
Unique spectral properties of quantum dots (QDs) enable ultrasensitive and long-term biolabeling. Aiming to trace the infection, movement, and localization of viruses in living cells, QD-containing virus-like particles (VLPs) of simian virus 40 (SV40), termed SVLP-QDs, are constructed by in vitro self-assembly of the major capsid protein of SV40. SVLP-QDs show homogeneity in size ( approximately 24 nm), similarity in spectral properties to unencapsidated QDs, and considerable stability. When incubated with living cells, SVLP-QDs are shown to enter the cells by caveolar endocytosis, travel along the microtubules, and accumulate in the endoplasmic reticulum. This process mimics the early infection steps of SV40. This is the first paradigm of imaging viral behaviors with encapsidated QDs in living cells. The method may provide a new alternative for various purposes, such as tracing viruses or viral components, targeted nanoparticle delivery, and probing of drug delivery.
Asunto(s)
Cápside/ultraestructura , Aumento de la Imagen/métodos , Microscopía Fluorescente/métodos , Puntos Cuánticos , Virus 40 de los Simios/fisiología , Virus 40 de los Simios/ultraestructura , Células Vero/citología , Animales , Cápside/química , Chlorocebus aethiopsRESUMEN
Bimolecular fluorescence complementation (BiFC) is a recently developed technique for detection of protein-protein interactions in living cells. In this study, a new red BiFC system was developed by splitting mCherry, a mutant monomeric red fluorescent protein, into two fragments between amino acids 159-160 and was verified using a pair of interacting proteins, SV40 large T antigen (LTag), and human p53 protein. By combined use of the mCherry-based red BiFC system with a Venus-based yellow BiFC system, the interaction between LTag and p53 as well as the interaction between sp100 and promyelocytic leukemia protein (PML), were detected simultaneously in Vero cells. The brilliant redness, short maturation time, and the long excitation and emission wavelengths (587/610 nm) of mCherry make the new BiFC system an excellent candidate for analyzing protein-protein interactions in living cells and for studying multiple protein-protein interactions when coupled with other BiFC systems.
Asunto(s)
Técnicas Biosensibles/métodos , Transferencia Resonante de Energía de Fluorescencia/métodos , Colorantes Fluorescentes/química , Proteínas Luminiscentes/química , Secuencia de Aminoácidos , Animales , Antígenos Transformadores de Poliomavirus/química , Antígenos Transformadores de Poliomavirus/metabolismo , Secuencia de Bases , Células Cultivadas/metabolismo , Células Cultivadas/patología , Chlorocebus aethiops , Humanos , Leucemia Promielocítica Aguda/metabolismo , Leucemia Promielocítica Aguda/patología , Datos de Secuencia Molecular , Proteína p53 Supresora de Tumor/química , Proteína p53 Supresora de Tumor/metabolismo , Células Vero/metabolismo , Células Vero/patología , Proteína Fluorescente RojaRESUMEN
Poliovirus RNA replication is directed by a replication complex on the rosette-like arrangement of membranous vesicles. Proteins derived from the p3 region of the polioviral genome, such as 3D, 3AB, and 3B (VPg), play key roles in the formation and function of the replication complex. In the present study, by using an acceptor photobleaching protocol for fluorescence resonance energy transfer (FRET) imaging, we visualized the interactions of 3D, 3AB, and VPg in living cells. The interaction of 3AB-VPg was determined by live cell FRET analysis. Quantitative analyses showed that the FRET efficiencies of 3AB-3D, VPg-3D, and 3AB-VPg were 3.9+/-0.4% (n=36), 4.5+/-0.4% (n=39), and 8.3+/-0.6% (n=44), respectively, in the cell cytoplasm where viral replication complexes are formed and function. Poliovirus infection enhanced the protein interactions of VPg-3D and 3AB-3D, with FRET efficiencies in the virus-infected cells of 10.7+/-1.1% (n=39) and 9.0+/-0.9% (n=37), respectively. This method of live cell analysis of protein interactions in the poliovirus RNA replication complex lays the foundation for further understanding of the real-time process of poliovirus RNA replication.
Asunto(s)
Transferencia Resonante de Energía de Fluorescencia/métodos , Poliovirus/fisiología , Mapeo de Interacción de Proteínas/métodos , ARN Viral/metabolismo , Proteínas de Unión al ARN/metabolismo , Proteínas Virales/metabolismo , Replicación Viral/fisiología , Animales , Chlorocebus aethiops , Poliovirus/ultraestructura , Células VeroRESUMEN
Dynamic analysis of viral nucleic acids in host cells is important for understanding virus-host interaction. By labeling endogenous RNA with molecular beacon, we have realized the direct visualization of viral nucleic acids in living host cells and have studied the dynamic behavior of poliovirus plus-strand RNA. Poliovirus plus-strand RNA was observed to display different distribution patterns in living Vero cells at different post-infection time points. Real-time imaging suggested that the translocation of poliovirus plus-strand RNA is a characteristic rearrangement process requiring intact microtubule network of host cells. Confocal-FRAP measurements showed that 49.4 +/- 3.2% of the poliovirus plus-strand RNA molecules diffused freely (with a D-value of 9.6 +/- 1.6 x 10(-10) cm2/s) within their distribution region, while the remaining (50.5 +/- 2.9%) were almost immobile and moved very slowly only with change of the RNA distribution region. Under the electron microscope, it was found that virus-induced membrane rearrangement is microtubule-associated in poliovirus-infected Vero cells. These results reveal an entrapment and diffusion mechanism for the movement of poliovirus plus-strand RNA in living mammalian cells, and demonstrate that the mechanism is mainly associated with microtubules and virus-induced membrane structures.
Asunto(s)
Poliovirus/genética , ARN Viral/análisis , Animales , Transporte Biológico , Chlorocebus aethiops , Citoplasma/virología , Vesículas Citoplasmáticas/ultraestructura , Difusión , Recuperación de Fluorescencia tras Fotoblanqueo , Hibridación Fluorescente in Situ , Microscopía Confocal , Microtúbulos/fisiología , Microtúbulos/ultraestructura , Sondas de Oligonucleótidos , ARN Viral/metabolismo , Células VeroRESUMEN
The self-assembly of nanoparticles into larger superstructures is a powerful strategy to develop novel functional nanomaterials, as these superstructures display collective properties that are different to those displayed by individual nanoparticles or bulk samples. However, there are increasing bottlenecks in terms of size control and multifunctionalization of nanoparticle assemblies. In this study, we developed a self-assembly strategy for construction of multifunctional nanoparticle assemblies of tunable size, through rational regulation of the number of self-assembling interaction sites on each nanoparticle. As proof-of-principle, a size-controlled enzyme nanocomposite (ENC) was constructed by self-assembly of streptavidin-labeled horseradish peroxidase (SA-HRP) and autobiotinylated ferritin nanoparticles (bFNP). Our ENC integrates a large number of enzyme molecules, together with a streptavidin-coated surface, allowing for a drastic increase in enzymatic signal when the SA is bound to a biotinylated target molecule. As result, a 10â¯000-fold increase in sensitivity over conventional enzyme-linked immunosorbent assays (ELISA) methods was achieved in a cardiac troponin immunoassay. Our method presented here should provide a feasible approach for constructing elaborate multifunctional superstructures of tunable size useful for a broad range of biomedical applications.
Asunto(s)
Ferritinas/química , Peroxidasa de Rábano Silvestre/metabolismo , Inmunoensayo/métodos , Nanocompuestos/química , Nanopartículas/química , Nanotecnología/métodos , Tamaño de la Partícula , Biotinilación , Ensayo de Inmunoadsorción Enzimática , Humanos , Nanocompuestos/ultraestructura , Nanopartículas/ultraestructura , Proteínas Recombinantes/química , Coloración y Etiquetado , Estreptavidina/metabolismo , Troponina I/metabolismoRESUMEN
Viruses encapsulating inorganic nanoparticles are a novel type of nanostructure with applications in biomedicine and biosensors. However, the encapsulation and assembly mechanisms of these hybridized virus-based nanoparticles (VNPs) are still unknown. In this article, it was found that quantum dots (QDs) can induce simian virus 40 (SV40) capsid assembly in dissociation buffer, where viral capsids should be disassembled. The analysis of the transmission electron microscope, dynamic light scattering, sucrose density gradient centrifugation, and cryo-electron microscopy single particle reconstruction experimental results showed that the SV40 major capsid protein 1 (VP1) can be assembled into ≈25 nm capsids in the dissociation buffer when QDs are present and that the QDs are encapsulated in the SV40 capsids. Moreover, it was determined that there is a strong affinity between QDs and the SV40 VP1 proteins (KD=2.19E-10 M), which should play an important role in QD encapsulation in the SV40 viral capsids. This study provides a new understanding of the assembly mechanism of SV40 virus-based nanoparticles with QDs, which may help in the design and construction of other similar virus-based nanoparticles.
Asunto(s)
Proteínas de la Cápside , Cápside , Nanopartículas , Puntos Cuánticos , Tampones (Química) , Cápside/química , Cápside/metabolismo , Cápside/ultraestructura , Proteínas de la Cápside/química , Proteínas de la Cápside/metabolismo , Proteínas de la Cápside/ultraestructura , Nanopartículas/química , Nanopartículas/ultraestructura , Tamaño de la Partícula , Virus 40 de los SimiosRESUMEN
There is an urgent need for convenient, sensitive, and specific methods to detect the spores of Bacillus anthracis, the causative agent of anthrax, because of the bioterrorism threat posed by this bacterium. In this study, we firstly develop a super-paramagnetic lateral-flow immunological detection system for B. anthracis spores. This system involves the use of a portable magnetic assay reader, super-paramagnetic iron oxide particles, lateral-flow strips and two different monoclonal antibodies directed against B. anthracis spores. This detection system specifically recognises as few as 400 pure B. anthracis spores in 30 min. This system has a linear range of 4×10³-106 CFU ml⻹ and reproducible detection limits of 200 spores mg⻹ milk powder and 130 spores mg⻹ soil for simulated samples. In addition, this approach shows no obvious cross-reaction with other related Bacillus spores, even at high concentrations, and has no significant dependence on the duration of the storage of the immunological strips. Therefore, this super-paramagnetic lateral-flow immunological detection system is a promising tool for the rapid and sensitive detection of Bacillus anthracis spores under field conditions.
Asunto(s)
Carbunco/microbiología , Bacillus anthracis/aislamiento & purificación , Bioterrorismo , Inmunoensayo/métodos , Esporas Bacterianas/aislamiento & purificación , Animales , Anticuerpos Monoclonales/química , Bacillus anthracis/inmunología , Bacillus anthracis/patogenicidad , Reacciones Cruzadas/inmunología , Leche/microbiología , Sensibilidad y Especificidad , Microbiología del Suelo , Esporas Bacterianas/inmunología , Esporas Bacterianas/patogenicidadRESUMEN
CobB is a bacterial NAD(+)-dependent protein deacetylase. Although progress has been made in functional studies of this protein in recent years, its substrates and biological functions are still largely unclear. Using proteome microarray technology, potential substrates of Escherichia coli CobB were screened and nine proteins were identified, including N-hydroxyarylamine O-acetyltransferase (NhoA). In vitro acetylation/deacetylation of NhoA was verified by western blotting and mass spectrometry, and two acetylated lysine residues were identified. Site-specific mutagenesis experiments showed that mutation of each acetylated lysine decreased the acetylation level of NhoA in vitro. Further analysis showed that variant NhoA proteins carrying substitutions at the two acetylated lysine residues are involved in both the O-acetyltransferase and N-acetyltransferase activity of NhoA. Structural analyses were also performed to explore the effects of the acetylated lysine residues on the activity of NhoA. These results suggest that reversible acetylation may play a role in the activity of Escherichia coli NhoA.
Asunto(s)
Acetiltransferasas/metabolismo , Proteínas de Escherichia coli/metabolismo , Escherichia coli/enzimología , Procesamiento Proteico-Postraduccional , Sirtuinas/metabolismo , Acetilación , Acetiltransferasas/química , Acetiltransferasas/genética , Secuencia de Aminoácidos , Sustitución de Aminoácidos , Proteínas de Escherichia coli/química , Cinética , Lisina/análogos & derivados , Datos de Secuencia Molecular , Mutagénesis Sitio-Dirigida , Fragmentos de Péptidos/química , Análisis por Matrices de Proteínas , Proteoma/metabolismo , Sirtuinas/químicaRESUMEN
Bionanoparticles and nanostructures have attracted increasing interest as versatile and promising tools in many applications including biosensing and bioimaging. In this study, to image and detect tumor cells, ferritin cage-based multifunctional hybrid nanostructures were constructed that: (i) displayed both the green fluorescent protein and an Arg-Gly-Asp peptide on the exterior surface of the ferritin cages; and (ii) incorporated ferrimagnetic iron oxide nanoparticles into the ferritin interior cavity. The overall architecture of ferritin cages did not change after being integrated with fusion proteins and ferrimagnetic iron oxide nanoparticles. These multifunctional nanostructures were successfully used as a fluorescent imaging probe and an MRI contrast agent for specifically probing and imaging α(v)ß(3) integrin upregulated tumor cells. The work provides a promising strategy for tumor cell detection by simultaneous fluorescence and MR imaging.
Asunto(s)
Medios de Contraste/química , Ferritinas/química , Nanoestructuras/química , Línea Celular Tumoral , Ferritinas/genética , Ferritinas/metabolismo , Humanos , Imagen por Resonancia Magnética , Microscopía Fluorescente , Oligopéptidos/química , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismoRESUMEN
The fluorescence labeling of viruses is a useful technology for virus detection and imaging. By combining the excellent fluorescence properties of quantum dots (QDs) with the high affinity and specificity of aptamers, we constructed a QD-aptamer probe. The aptamer A22, against the hemagglutinin of influenza A virus, was linked to QDs, producing the QD-A22 probe. Fluorescence imaging and transmission electron microscopy showed that the QD-A22 probe could specifically recognize and label influenza A virus particles. This QD labeling technique provides a new strategy for labeling virus particles for virus detection and imaging.
Asunto(s)
Aptámeros de Nucleótidos/química , Virus de la Influenza A/química , Puntos Cuánticos , Aptámeros de Nucleótidos/metabolismo , Electroforesis en Gel de Poliacrilamida , Virus de la Influenza A/aislamiento & purificación , Virus de la Influenza A/metabolismo , Microscopía Electrónica de Transmisión , Microscopía FluorescenteRESUMEN
Carrageenan polysaccharide has been reported to be able to inhibit the infection and replication of many different kinds of viruses. Here, we demonstrated that a 2 kDa κ-carrageenan oligosaccharide (CO-1) derived from the carrageenan polysaccharide, effectively inhibited influenza A (H1N1) virus replication in MDCK cells (selectivity index >25.0). Moreover, the 2 kDa CO-1 inhibited influenza A virus (IAV) replication better than that of 3 kDa and 5 kDa κ-carrageenan oligosaccharides (CO-2 and CO-3). IAV multiplication was suppressed by carrageenan oligosaccharide treatment in a dose-dependent manner. Carrageenan oligosaccharide CO-1 did not bind to the cell surface of MDCK cells but inactivated virus particles after pretreatment. Different to the actions of carrageenan polysaccharide, CO-1 could enter into MDCK cells and did not interfere with IAV adsorption. CO-1 also inhibited IAV mRNA and protein expression after its internalization into cells. Moreover, carrageenan oligosaccharide CO-1 had an antiviral effect on IAV replication subsequent to viral internalization but prior to virus release in one replication cycle. Therefore, inhibition of IAV intracellular replication by carrageenan oligosaccharide might be an alternative approach for anti-influenza A virus therapy.